{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T15:28:24Z","timestamp":1786980504488,"version":"build-2736575974"},"reference-count":41,"publisher":"Springer Science and Business Media LLC","issue":"8","license":[{"start":{"date-parts":[[2024,6,4]],"date-time":"2024-06-04T00:00:00Z","timestamp":1717459200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2024,6,4]],"date-time":"2024-06-04T00:00:00Z","timestamp":1717459200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key R&D Program of China","doi-asserted-by":"crossref","award":["2022YFC2603800"],"award-info":[{"award-number":["2022YFC2603800"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100012166","name":"National Key R&D Program of China","doi-asserted-by":"crossref","award":["2022YFC2603800"],"award-info":[{"award-number":["2022YFC2603800"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100012166","name":"National Key R&D Program of China","doi-asserted-by":"crossref","award":["2022YFC2603800"],"award-info":[{"award-number":["2022YFC2603800"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100012166","name":"National Key R&D Program of China","doi-asserted-by":"crossref","award":["2022YFC2603800"],"award-info":[{"award-number":["2022YFC2603800"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100012166","name":"National Key R&D Program of China","doi-asserted-by":"crossref","award":["2022YFC2603800"],"award-info":[{"award-number":["2022YFC2603800"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100012166","name":"National Key R&D Program of China","doi-asserted-by":"crossref","award":["2022YFC2603800"],"award-info":[{"award-number":["2022YFC2603800"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100012166","name":"National Key R&D Program of China","doi-asserted-by":"crossref","award":["2022YFC2603800"],"award-info":[{"award-number":["2022YFC2603800"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100012166","name":"National Key R&D Program of China","doi-asserted-by":"crossref","award":["2022YFC2603800"],"award-info":[{"award-number":["2022YFC2603800"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100012166","name":"National Key R&D Program of China","doi-asserted-by":"crossref","award":["2022YFC2603800"],"award-info":[{"award-number":["2022YFC2603800"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100012166","name":"National Key R&D Program of China","doi-asserted-by":"crossref","award":["2022YFC2603800"],"award-info":[{"award-number":["2022YFC2603800"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100012166","name":"National Key R&D Program of China","doi-asserted-by":"crossref","award":["2022YFC2603800"],"award-info":[{"award-number":["2022YFC2603800"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100012166","name":"National Key R&D Program of China","doi-asserted-by":"crossref","award":["2022YFC2603800"],"award-info":[{"award-number":["2022YFC2603800"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100012166","name":"National Key R&D Program of China","doi-asserted-by":"crossref","award":["2022YFC2603800"],"award-info":[{"award-number":["2022YFC2603800"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100012166","name":"National Key R&D Program of China","doi-asserted-by":"crossref","award":["2022YFC2603800"],"award-info":[{"award-number":["2022YFC2603800"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100012166","name":"National Key R&D Program of China","doi-asserted-by":"crossref","award":["2022YFC2603800"],"award-info":[{"award-number":["2022YFC2603800"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Eur J Clin Microbiol Infect Dis"],"published-print":{"date-parts":[[2024,8]]},"DOI":"10.1007\/s10096-024-04865-0","type":"journal-article","created":{"date-parts":[[2024,6,4]],"date-time":"2024-06-04T06:29:28Z","timestamp":1717482568000},"page":"1597-1607","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Diagnostic performance of mpox virus (MPXV) real-time PCR assays: multicenter assessment and extended sensitivity analysis"],"prefix":"10.1007","volume":"43","author":[{"given":"Ziqiang","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuqing","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanxi","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenli","family":"Diao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cong","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Tian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanyu","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zihong","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinming","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,6,4]]},"reference":[{"issue":"5","key":"4865_CR1","first-page":"593","volume":"46","author":"ID Ladnyj","year":"1972","unstructured":"Ladnyj ID, Ziegler P, Kima E (1972) A human infection caused by monkeypox virus in Basankusu Territory, Democratic Republic of the Congo. Bull World Health Organ 46(5):593\u2013597","journal-title":"Bull World Health Organ"},{"issue":"10370","key":"4865_CR2","doi-asserted-by":"publisher","first-page":"60","DOI":"10.1016\/s0140-6736(22)02075-x","volume":"401","author":"O Mitj\u00e0","year":"2023","unstructured":"Mitj\u00e0 O, Ogoina D, Titanji BK, Galvan C, Muyembe JJ, Marks M, Orkin CM, Monkeypox (2023) Lancet (London England) 401(10370):60\u201374. https:\/\/doi.org\/10.1016\/s0140-6736(22)02075-x","journal-title":"Lancet (London England)"},{"key":"4865_CR3","unstructured":"2022\u20132023 Mpox Outbreak Global Map. https:\/\/www.cdc.gov\/poxvirus\/mpox\/response\/2022\/world-map.html.\u00a0Accessed 28 Apr 2024"},{"issue":"4","key":"4865_CR4","doi-asserted-by":"publisher","first-page":"e28701","DOI":"10.1002\/jmv.28701","volume":"95","author":"D Aden","year":"2023","unstructured":"Aden D, Zaheer S, Kumar R, Ranga S (2023) Monkeypox (Mpox) outbreak during COVID-19 pandemic-past and the future. J Med Virol 95(4):e28701. https:\/\/doi.org\/10.1002\/jmv.28701","journal-title":"J Med Virol"},{"key":"4865_CR5","unstructured":"WHO Director-General declares the ongoing monkeypox outbreak a Public Health Emergency of International Concern.\u00a0https:\/\/www.who.int\/europe\/news\/item\/23-07-2022-who-director-general-declares-the-ongoing-monkeypox-outbreak-a-public-health-event-of-international-concern.\u00a0Accessed 28 Apr 2024"},{"key":"4865_CR6","doi-asserted-by":"publisher","unstructured":"Gigante CM, Korber B, Seabolt MH, Wilkins K, Davidson W, Rao AK, Zhao H, Smith TG, Hughes CM, Minhaj F et al (2022) Multiple lineages of monkeypox virus detected in the United States, 2021\u20132022. Science 378(6619):560\u2013565. https:\/\/doi.org\/10.1126\/science.add4153","DOI":"10.1126\/science.add4153"},{"key":"4865_CR7","doi-asserted-by":"publisher","first-page":"1174223","DOI":"10.3389\/fimmu.2023.1174223","volume":"14","author":"Y Wang","year":"2023","unstructured":"Wang Y, Leng P, Zhou H (2023) Global transmission of monkeypox virus-a potential threat under the COVID-19 pandemic. Front Immunol 14:1174223. https:\/\/doi.org\/10.3389\/fimmu.2023.1174223","journal-title":"Front Immunol"},{"issue":"10353","key":"4865_CR8","doi-asserted-by":"publisher","first-page":"661","DOI":"10.1016\/s0140-6736(22)01436-2","volume":"400","author":"EJ Tar\u00edn-Vicente","year":"2022","unstructured":"Tar\u00edn-Vicente EJ, Alemany A, Agud-Dios M, Ubals M, Su\u00f1er C, Ant\u00f3n A, Arando M, Arroyo-Andr\u00e9s J, Calder\u00f3n-Lozano L, Casa\u00f1 C et al (2022) Clinical presentation and virological assessment of confirmed human monkeypox virus cases in Spain: a prospective observational cohort study. Lancet (London England) 400(10353):661\u2013669. https:\/\/doi.org\/10.1016\/s0140-6736(22)01436-2","journal-title":"Lancet (London England)"},{"key":"4865_CR9","doi-asserted-by":"publisher","unstructured":"Liu BM, Rakhmanina NY, Yang Z, Bukrinsky MI (2024) Mpox (Monkeypox) virus and its co-infection with HIV, sexually transmitted infections, or bacterial superinfections: double whammy or a new prime culprit?\u00a0Viruses 16(5):784. https:\/\/doi.org\/10.3390\/v16050784","DOI":"10.3390\/v16050784"},{"key":"4865_CR10","doi-asserted-by":"publisher","unstructured":"Sun H, Miao Y, Yang X, Guo L, Li Q, Wang J, Long J, Zhang Z, Shi J, Li J et al (2024) Rapid identification of A29L antibodies based on mRNA immunization and high-throughput single B cell sequencing to detect Monkeypox virus. Emerg Microbes Infect 13(1):2332665. https:\/\/doi.org\/10.1080\/22221751.2024.2332665","DOI":"10.1080\/22221751.2024.2332665"},{"key":"4865_CR11","doi-asserted-by":"publisher","unstructured":"Li Y, Olson VA, Laue T, Laker MT, Damon IK (2006) Detection of monkeypox virus with real-time PCR assays. J Clin Virol: Off Publ Pan Amer Soc Clin Virol 36(3):194\u2013203. https:\/\/doi.org\/10.1016\/j.jcv.2006.03.012","DOI":"10.1016\/j.jcv.2006.03.012"},{"key":"4865_CR12","doi-asserted-by":"publisher","unstructured":"Hartwell LH, Hopfield JJ, Leibler S, Murray AW (1999) From molecular to modular cell biology. Nature 402(6761 Suppl):C47\u201352. https:\/\/doi.org\/10.1038\/35011540","DOI":"10.1038\/35011540"},{"key":"4865_CR13","doi-asserted-by":"publisher","first-page":"113541","DOI":"10.1016\/j.bios.2021.113541","volume":"193","author":"L Yin","year":"2021","unstructured":"Yin L, Man S, Ye S, Liu G, Ma L (2021) CRISPR-Cas based virus detection: recent advances and perspectives. Biosens Bioelectron 193:113541. https:\/\/doi.org\/10.1016\/j.bios.2021.113541","journal-title":"Biosens Bioelectron"},{"key":"4865_CR14","doi-asserted-by":"publisher","unstructured":"Guarner J, Johnson BJ, Paddock CD, Shieh WJ, Goldsmith CS, Reynolds MG, Damon IK, Regnery RL, Zaki SR (2004) Monkeypox transmission and pathogenesis in prairie dogs. Emerg Infect Dis 10(3):426\u2013431. https:\/\/doi.org\/10.3201\/eid1003.030878","DOI":"10.3201\/eid1003.030878"},{"key":"4865_CR15","unstructured":"Laboratory testing for the monkeypox virus: interim guidance. https:\/\/www.who.int\/publications\/i\/item\/who-mpx-laboratory-2023-1.\u00a0Accessed 28 Apr 2024"},{"issue":"1","key":"4865_CR16","doi-asserted-by":"publisher","first-page":"e28429","DOI":"10.1002\/jmv.28429","volume":"95","author":"CK Lim","year":"2023","unstructured":"Lim CK, Roberts J, Moso M, Liew KC, Taouk ML, Williams E, Tran T, Steinig E, Caly L, Williamson DA (2023) Mpox diagnostics: review of current and emerging technologies. J Med Virol 95(1):e28429. https:\/\/doi.org\/10.1002\/jmv.28429","journal-title":"J Med Virol"},{"key":"4865_CR17","doi-asserted-by":"publisher","unstructured":"Antinori A, Mazzotta V, Vita S, Carletti F, Tacconi D, Lapini LE, D\u2019Abramo A, Cicalini S, Lapa D, Pittalis S et al (2022) Epidemiological, clinical and virological characteristics of four cases of monkeypox support transmission through sexual contact, Italy, May 2022. Euro Surveill 27(22):2200421.\u00a0https:\/\/doi.org\/10.2807\/1560-7917.Es.2022.27.22.2200421","DOI":"10.2807\/1560-7917.Es.2022.27.22.2200421"},{"key":"4865_CR18","doi-asserted-by":"publisher","unstructured":"Thornhill JP, Barkati S, Walmsley S, Rockstroh J, Antinori A, Harrison LB, Palich R, Nori A, Reeves I, Habibi MS et al (2022) Monkeypox Virus infection in humans across 16 countries - April-June 2022. N Engl J Med 387(8):679\u2013691. https:\/\/doi.org\/10.1056\/NEJMoa2207323","DOI":"10.1056\/NEJMoa2207323"},{"issue":"8","key":"4865_CR19","doi-asserted-by":"publisher","first-page":"1153","DOI":"10.1016\/s1473-3099(22)00228-6","volume":"22","author":"H Adler","year":"2022","unstructured":"Adler H, Gould S, Hine P, Snell LB, Wong W, Houlihan CF, Osborne JC, Rampling T, Beadsworth MB, Duncan CJ et al (2022) Clinical features and management of human monkeypox: a retrospective observational study in the UK. Lancet Infect Dis 22(8):1153\u20131162. https:\/\/doi.org\/10.1016\/s1473-3099(22)00228-6","journal-title":"Lancet Infect Dis"},{"key":"4865_CR20","doi-asserted-by":"publisher","unstructured":"Moschese D, Pozza G, Mileto D, Giacomelli A, Cutrera M, Cossu MV, Matone M, Beltrami M, Salari F, Antinori S et al (2022) Isolation of viable monkeypox virus from anal and urethral swabs, Italy, May to July 2022.\u00a0Euro Surveill 27(36):2200675.\u00a0https:\/\/doi.org\/10.2807\/1560-7917.Es.2022.27.36.2200675","DOI":"10.2807\/1560-7917.Es.2022.27.36.2200675"},{"issue":"3","key":"4865_CR21","doi-asserted-by":"publisher","first-page":"e168","DOI":"10.1016\/s2666-5247(22)00003-9","volume":"3","author":"C Buchta","year":"2022","unstructured":"Buchta C, M\u00fcller MM, Griesmacher A (2022) The importance of external quality assessment data in evaluating SARS-CoV-2 virus genome detection assays. Lancet Microbe 3(3):e168. https:\/\/doi.org\/10.1016\/s2666-5247(22)00003-9","journal-title":"Lancet Microbe"},{"issue":"17","key":"4865_CR22","doi-asserted-by":"publisher","first-page":"7047","DOI":"10.1007\/s00253-015-6664-4","volume":"99","author":"L Zhang","year":"2015","unstructured":"Zhang L, Sun Y, Chang L, Jia T, Wang G, Zhang R, Zhang K, Li J (2015) A novel method to produce armored double-stranded DNA by encapsulation of MS2 viral capsids. Appl Microbiol Biotechnol 99(17):7047\u20137057. https:\/\/doi.org\/10.1007\/s00253-015-6664-4","journal-title":"Appl Microbiol Biotechnol"},{"key":"4865_CR23","doi-asserted-by":"publisher","unstructured":"Mills MG, Juergens KB, Gov JP, McCormick CJ, Sampoleo R, Kachikis A, Amory JK, Fang FC, P\u00e9rez-Osorio AC, Lieberman NAP et al (2023) Evaluation and clinical validation of monkeypox (mpox) virus real-time PCR assays. J Clin Virol\u00a0159:105373. https:\/\/doi.org\/10.1016\/j.jcv.2022.105373","DOI":"10.1016\/j.jcv.2022.105373"},{"issue":"Supplement2","key":"4865_CR24","doi-asserted-by":"publisher","first-page":"S137","DOI":"10.1093\/infdis\/jiad406","volume":"229","author":"M Anderson","year":"2024","unstructured":"Anderson M, Hodges A, Luk KC, Olivo A, Forberg K, Meyer TV, Strobel C, Kim M, Toolsie D, Moore NM et al (2024) Development and validation of three automated high-throughput molecular tests to detect Monkeypox Virus infections. J Infect Dis 229(Supplement2):S137\u2013s143. https:\/\/doi.org\/10.1093\/infdis\/jiad406","journal-title":"J Infect Dis"},{"key":"4865_CR25","doi-asserted-by":"publisher","unstructured":"Nuzzo JB, Borio LL, Gostin LO (2022) The WHO Declaration of Monkeypox as a Global Public Health Emergency. JAMA 328(7):615\u2013617. https:\/\/doi.org\/10.1001\/jama.2022.12513","DOI":"10.1001\/jama.2022.12513"},{"issue":"26","key":"4865_CR26","doi-asserted-by":"publisher","first-page":"2569","DOI":"10.1056\/NEJMsr1813907","volume":"381","author":"GL Armstrong","year":"2019","unstructured":"Armstrong GL, MacCannell DR, Taylor J, Carleton HA, Neuhaus EB, Bradbury RS, Posey JE, Gwinn M (2019) Pathogen Genomics in Public Health. N Engl J Med 381(26):2569\u20132580. https:\/\/doi.org\/10.1056\/NEJMsr1813907","journal-title":"N Engl J Med"},{"issue":"10326","key":"4865_CR27","doi-asserted-by":"publisher","first-page":"757","DOI":"10.1016\/s0140-6736(21)02346-1","volume":"399","author":"RW Peeling","year":"2022","unstructured":"Peeling RW, Heymann DL, Teo YY, Garcia PJ (2022) Diagnostics for COVID-19: moving from pandemic response to control. Lancet (London England) 399(10326):757\u2013768. https:\/\/doi.org\/10.1016\/s0140-6736(21)02346-1","journal-title":"Lancet (London England)"},{"key":"4865_CR28","doi-asserted-by":"publisher","unstructured":"Dieffenbach CW, Lowe TM, Dveksler GS (1993) General concepts for PCR primer design. PCR Methods Appl 3(3):S30-37 https:\/\/doi.org\/10.1101\/gr.3.3.s30","DOI":"10.1101\/gr.3.3.s30"},{"key":"4865_CR29","doi-asserted-by":"publisher","first-page":"339338","DOI":"10.1016\/j.aca.2021.339338","volume":"1209","author":"MM Islam","year":"2022","unstructured":"Islam MM, Koirala D (2022) Toward a next-generation diagnostic tool: a review on emerging isothermal nucleic acid amplification techniques for the detection of SARS-CoV-2 and other infectious viruses. Anal Chim Acta 1209:339338. https:\/\/doi.org\/10.1016\/j.aca.2021.339338","journal-title":"Anal Chim Acta"},{"key":"4865_CR30","doi-asserted-by":"publisher","unstructured":"Jang M, Kim S, Song J, Kim S (2022) Rapid and simple detection of influenza virus via isothermal amplification lateral flow assay. Anal Bioanal Chem 414(16):4685\u20134696. https:\/\/doi.org\/10.1007\/s00216-022-04090-8","DOI":"10.1007\/s00216-022-04090-8"},{"issue":"3","key":"4865_CR31","doi-asserted-by":"publisher","first-page":"415","DOI":"10.1080\/07388551.2022.2030295","volume":"43","author":"Z Wei","year":"2023","unstructured":"Wei Z, Wang X, Feng H, Ji F, Bai D, Dong X, Huang W (2023) Isothermal nucleic acid amplification technology for rapid detection of virus. Crit Rev Biotechnol 43(3):415\u2013432. https:\/\/doi.org\/10.1080\/07388551.2022.2030295","journal-title":"Crit Rev Biotechnol"},{"key":"4865_CR32","doi-asserted-by":"publisher","unstructured":"Michel J, Targosz A, Rinner T, Bourquain D, Brinkmann A, Sacks JA, Schaade L, Nitsche A (2022) Evaluation of 11 commercially available PCR kits for the detection of monkeypox virus DNA, Berlin, July to September 2022. Euro Surveill\u00a027(45):2200816.\u00a0https:\/\/doi.org\/10.2807\/1560-7917.Es.2022.27.45.2200816","DOI":"10.2807\/1560-7917.Es.2022.27.45.2200816"},{"key":"4865_CR33","unstructured":"Impact of false-positives and false-negatives in the UK\u2019s COVID-19 RT-PCR testing programme. https:\/\/assets.publishing.service.gov.uk\/media\/5ef5de1486650c1295cb5ed2\/S0519_Impact_of_false_positives_and_negatives.pdf.\u00a0Accessed 28 Apr 2024"},{"issue":"2","key":"4865_CR34","doi-asserted-by":"publisher","first-page":"e0127523","DOI":"10.1128\/jcm.01275-23","volume":"62","author":"RC Shean","year":"2024","unstructured":"Shean RC, Hymas WC, Klein J, Pyne M, Hillyard DR, Bradley BT (2024) Repeat testing of mpox specimens with late CTs improves detection of potential false positive cases. J Clin Microbiol 62(2):e0127523. https:\/\/doi.org\/10.1128\/jcm.01275-23","journal-title":"J Clin Microbiol"},{"issue":"4","key":"4865_CR35","doi-asserted-by":"publisher","first-page":"559","DOI":"10.1128\/cmr.13.4.559","volume":"13","author":"EM Elnifro","year":"2000","unstructured":"Elnifro EM, Ashshi AM, Cooper RJ, Klapper PE (2000) Multiplex PCR: optimization and application in diagnostic virology. Clin Microbiol Rev 13(4):559\u2013570. https:\/\/doi.org\/10.1128\/cmr.13.4.559","journal-title":"Clin Microbiol Rev"},{"key":"4865_CR36","doi-asserted-by":"publisher","unstructured":"Xu J, Wang J, Su X, Qiu G, Zhong Q, Li T, Zhang D, Zhang S, He S, Ge S et al (2021) Transferable, easy-to-use and room-temperature-storable PCR mixes for microfluidic molecular diagnostics. Talanta 235:122797. https:\/\/doi.org\/10.1016\/j.talanta.2021.122797","DOI":"10.1016\/j.talanta.2021.122797"},{"issue":"38","key":"4865_CR37","doi-asserted-by":"publisher","first-page":"11870","DOI":"10.1073\/pnas.1515692112","volume":"112","author":"W Deng","year":"2015","unstructured":"Deng W, Shi X, Tjian R, Lionnet T, Singer RH (2015) CASFISH: CRISPR\/Cas9-mediated in situ labeling of genomic loci in fixed cells. Proc Natl Acad Sci USA 112(38):11870\u201311875. https:\/\/doi.org\/10.1073\/pnas.1515692112","journal-title":"Proc Natl Acad Sci USA"},{"issue":"6","key":"4865_CR38","doi-asserted-by":"publisher","first-page":"e45","DOI":"10.1093\/nar\/gkp045","volume":"37","author":"JM Ruijter","year":"2009","unstructured":"Ruijter JM, Ramakers C, Hoogaars WM, Karlen Y, Bakker O, van den Hoff MJ, Moorman AF (2009) Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR data. Nucleic Acids Res 37(6):e45. https:\/\/doi.org\/10.1093\/nar\/gkp045","journal-title":"Nucleic Acids Res"},{"key":"4865_CR39","doi-asserted-by":"publisher","unstructured":"Su\u00f1er C, Ubals M, Tar\u00edn-Vicente EJ, Mendoza A, Alemany A, Hern\u00e1ndez-Rodr\u00edguez \u00c1, Casa\u00f1 C, Descalzo V, Ouchi D, Marc A et al (2023) Viral dynamics in patients with monkeypox infection: a prospective cohort study in Spain. Lancet Infect Dis 23(4):445\u2013453. https:\/\/doi.org\/10.1016\/s1473-3099(22)00794-0","DOI":"10.1016\/s1473-3099(22)00794-0"},{"key":"4865_CR40","doi-asserted-by":"publisher","unstructured":"N\u00f6rz D, Brehm TT, Tang HT, Grewe I, Hermanussen L, Matthews H, Pestel J, Degen O, G\u00fcnther T, Grundhoff A et al (2022) Clinical characteristics and comparison of longitudinal qPCR results from different specimen types in a cohort of ambulatory and hospitalized patients infected with monkeypox virus. J Clin Virol: Off Pub Pan Amer Soc Clin Virol 155:105254. https:\/\/doi.org\/10.1016\/j.jcv.2022.105254","DOI":"10.1016\/j.jcv.2022.105254"},{"key":"4865_CR41","doi-asserted-by":"publisher","unstructured":"Liu B, Panda D, Mendez-Rios JD, Ganesan S, Wyatt LS, Moss B (2018) Identification of poxvirus genome uncoating and DNA replication factors with mutually redundant roles. J Virol 92(7):e02152-17.\u00a0https:\/\/doi.org\/10.1128\/jvi.02152-17","DOI":"10.1128\/jvi.02152-17"}],"container-title":["European Journal of Clinical Microbiology & Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10096-024-04865-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10096-024-04865-0\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10096-024-04865-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,20]],"date-time":"2024-07-20T15:02:49Z","timestamp":1721487769000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10096-024-04865-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6,4]]},"references-count":41,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2024,8]]}},"alternative-id":["4865"],"URL":"https:\/\/doi.org\/10.1007\/s10096-024-04865-0","relation":{},"ISSN":["0934-9723","1435-4373"],"issn-type":[{"value":"0934-9723","type":"print"},{"value":"1435-4373","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,6,4]]},"assertion":[{"value":"9 May 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"27 May 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"4 June 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}