{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T13:33:03Z","timestamp":1758807183851,"version":"3.37.3"},"reference-count":33,"publisher":"Springer Science and Business Media LLC","issue":"10","license":[{"start":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T00:00:00Z","timestamp":1722988800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T00:00:00Z","timestamp":1722988800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81861138055"],"award-info":[{"award-number":["81861138055"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"West China Hospital of Sichuan University","award":["312190022"],"award-info":[{"award-number":["312190022"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Eur J Clin Microbiol Infect Dis"],"published-print":{"date-parts":[[2024,10]]},"DOI":"10.1007\/s10096-024-04910-y","type":"journal-article","created":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T11:07:15Z","timestamp":1723028835000},"page":"2029-2035","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Characterization of a KPC-84 harboring Klebsiella pneumoniae ST11 clinical isolate with ceftazidime-avibactam resistance"],"prefix":"10.1007","volume":"43","author":[{"given":"Yanqiao","family":"Gong","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Feng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhiyong","family":"Zong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoju","family":"Lv","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,8,7]]},"reference":[{"key":"4910_CR1","doi-asserted-by":"publisher","unstructured":"Ma J, Song X, Li M, Yu Z, Cheng W, Yu Z et al Global spread of carbapenem-resistant Enterobacteriaceae: epidemiological features, resistance mechanisms, detection and therapy. Microbiol Res 2023 1;266:127249. https:\/\/doi.org\/10.1016\/j.micres.2022.127249","DOI":"10.1016\/j.micres.2022.127249"},{"key":"4910_CR2","doi-asserted-by":"publisher","first-page":"229","DOI":"10.1016\/S1473-3099(17)30748-X","volume":"18","author":"AC Kalil","year":"2018","unstructured":"Kalil AC, Klompas M (2018) Ceftazidime-Avibactam versus meropenem for the treatment of nosocomial pneumonia. LANCET Infect Dis 18:229\u2013231. https:\/\/doi.org\/10.1016\/S1473-3099(17)30748-X","journal-title":"LANCET Infect Dis"},{"key":"4910_CR3","doi-asserted-by":"publisher","first-page":"6605","DOI":"10.1128\/AAC.01165-15","volume":"59","author":"M Humphries Romney","year":"2015","unstructured":"Humphries Romney M, Yang S, Hemarajata P, Ward Kevin W, Hindler Janet A, Miller Shelley A et al (2015) First Report of Ceftazidime-Avibactam Resistance in a KPC-3-Expressing Klebsiella pneumoniae isolate. Antimicrob. Agents Chemother 59:6605\u20136607. https:\/\/doi.org\/10.1128\/AAC.01165-15","journal-title":"Agents Chemother"},{"key":"4910_CR4","doi-asserted-by":"publisher","first-page":"18","DOI":"10.1016\/j.jgar.2019.12.009","volume":"22","author":"Y Wang","year":"2020","unstructured":"Wang Y, Wang J, Wang R, Cai Y (2020) Resistance to ceftazidime-avibactam and underlying mechanisms. J Glob Antimicrob Resist 22:18\u201327. https:\/\/doi.org\/10.1016\/j.jgar.2019.12.009","journal-title":"J Glob Antimicrob Resist"},{"key":"4910_CR5","doi-asserted-by":"publisher","unstructured":"Hobson CA, Pierrat G, Tenaillon O, Bonacorsi S, Bercot B, Jaouen E et al Klebsiella pneumoniae carbapenemase variants resistant to Ceftazidime-Avibactam: an evolutionary overview. Antimicrob Agents Chemother 2022 20;66:e0044722. https:\/\/doi.org\/10.1128\/aac.00447-22","DOI":"10.1128\/aac.00447-22"},{"key":"4910_CR6","doi-asserted-by":"publisher","unstructured":"Shields RK, Chen L, Cheng S, Chavda KD, Press EG, Snyder A et al (2017) Emergence of Ceftazidime-Avibactam Resistance due to plasmid-borne blaKPC-3 mutations during treatment of Carbapenem-resistant Klebsiella pneumoniae infections. Antimicrob Agents Chemother 61. https:\/\/doi.org\/10.1128\/AAC.02097-16","DOI":"10.1128\/AAC.02097-16"},{"key":"4910_CR7","doi-asserted-by":"publisher","unstructured":"Garcia J, Nastro M, Cejas D, Santana G, Mancino MB, Hidalgo M et al (2020) 23;doi Emergence of ceftazidime\/avibactam resistance in KPC-8-producing Klebsiella pneumoniae in South America. CLINICAL MICROBIOLOGY AND INFECTION: https:\/\/doi.org\/10.1016\/j.cmi.2020.03.013","DOI":"10.1016\/j.cmi.2020.03.013"},{"key":"4910_CR8","doi-asserted-by":"publisher","unstructured":"Galani I, Antoniadou A, Karaiskos I, Kontopoulou K, Giamarellou H, Souli M Genomic characterization of a KPC-23-producing Klebsiella pneumoniae ST258 clinical isolate resistant to ceftazidime-avibactam. CLINICAL MICROBIOLOGY AND INFECTION 2019;25:763 e5-763 e8. https:\/\/doi.org\/10.1016\/j.cmi.2019.03.011","DOI":"10.1016\/j.cmi.2019.03.011"},{"key":"4910_CR9","doi-asserted-by":"publisher","first-page":"268","DOI":"10.1016\/j.jgar.2021.04.001","volume":"25","author":"S Di Bella","year":"2021","unstructured":"Di Bella S, Giacobbe DR, Maraolo AE, Viaggi V, Luzzati R, Bassetti M et al (2021) Resistance to ceftazidime\/avibactam in infections and colonisations by KPC-producing enterobacterales: a systematic review of observational clinical studies. J Global Antimicrob Resist 25:268\u2013281. https:\/\/doi.org\/10.1016\/j.jgar.2021.04.001","journal-title":"J Global Antimicrob Resist"},{"key":"4910_CR10","doi-asserted-by":"publisher","first-page":"e00955","DOI":"10.1128\/AAC.00955-19","volume":"63","author":"LS Munoz-Price","year":"2019","unstructured":"Munoz-Price LS, Reeme AE, Buchan BW, Mettus RT, Mustapha MM, Van Tyne D et al (2019) Patient-to-patient transmission of Klebsiella pneumoniae carbapenemase variants with reduced ceftazidime-Avibactam susceptibility. Antimicrob Agents Chemother 63:e00955\u2013e00919. https:\/\/doi.org\/10.1128\/AAC.00955-19","journal-title":"Antimicrob Agents Chemother"},{"key":"4910_CR11","doi-asserted-by":"publisher","unstructured":"Huang X, Shen S, Chang F, Liu X, Yue J, Xie N et al Emergence of KPC-134, a KPC-2 variant associated with ceftazidime-avibactam resistance in a ST11 Klebsiella pneumoniae clinical strain. Microbiol Spectr 2023 29;11:e0072523. https:\/\/doi.org\/10.1128\/spectrum.00725-23","DOI":"10.1128\/spectrum.00725-23"},{"key":"4910_CR12","doi-asserted-by":"publisher","unstructured":"Wu Y, Yang X, Liu C, Zhang Y, Cheung YC, Wai Chi Chan E et al Identification of a KPC variant conferring resistance to Ceftazidime-Avibactam from ST11 Carbapenem-resistant Klebsiella pneumoniae strains. Microbiol Spectr 2022 27;10:e0265521. https:\/\/doi.org\/10.1128\/spectrum.02655-21","DOI":"10.1128\/spectrum.02655-21"},{"key":"4910_CR13","doi-asserted-by":"publisher","first-page":"724272","DOI":"10.3389\/fmicb.2021.724272","volume":"12","author":"X Li","year":"2021","unstructured":"Li X, Quan J, Ke H, Wu W, Feng Y, Yu Y et al (2021) Emergence of a KPC variant conferring resistance to Ceftazidime-Avibactam in a widespread ST11 carbapenem-resistant Klebsiella pneumoniae clone in China. Front Microbiol 12:724272. https:\/\/doi.org\/10.3389\/fmicb.2021.724272","journal-title":"Front Microbiol"},{"key":"4910_CR14","doi-asserted-by":"publisher","unstructured":"Li X, Ke H, Wu W, Tu Y, Zhou H, Yu Y Molecular mechanisms driving the in vivo development of KPC-71-Mediated resistance to Ceftazidime-Avibactam during treatment of Carbapenem-resistant Klebsiella pneumoniae infections. mSphere 2021 22;6:e0085921. https:\/\/doi.org\/10.1128\/mSphere.00859-21","DOI":"10.1128\/mSphere.00859-21"},{"key":"4910_CR15","doi-asserted-by":"publisher","unstructured":"Hobson CA, Bonacorsi S, Jacquier H, Choudhury A, Magnan M, Cointe A et al (2020) 5;doi KPC beta-lactamases are permissive to insertions and deletions conferring substrate spectrum modifications and resistance to ceftazidime-avibactam. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY: https:\/\/doi.org\/10.1128\/AAC.01175-20","DOI":"10.1128\/AAC.01175-20"},{"key":"4910_CR16","unstructured":"The European Committee on Antimicrobial Susceptibility Testing (2024) Breakpoint tables for interpretation of MICs and zone diameters, version 14.0"},{"key":"4910_CR17","doi-asserted-by":"publisher","first-page":"1365","DOI":"10.3389\/fmicb.2020.01365","volume":"11","author":"X Cui","year":"2020","unstructured":"Cui X, Shan B, Zhang X, Qu F, Jia W, Huang B et al (2020) Reduced ceftazidime-Avibactam susceptibility in KPC-Producing Klebsiella pneumoniae from patients without Ceftazidime-Avibactam Use History - A Multicenter Study in China. Front Microbiol 11:1365. https:\/\/doi.org\/10.3389\/fmicb.2020.01365","journal-title":"Front Microbiol"},{"key":"4910_CR18","unstructured":"Using, SPAdes De Novo Assembler - Prjibelski \u2013\u20092020 - Current Protocols in Bioinformatics - Wiley Online Library [Internet]. [cited 2024 14];doi: https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/abs\/10.1002\/cpbi.102"},{"key":"4910_CR19","doi-asserted-by":"publisher","unstructured":"Jain C, Rodriguez-R LM, Phillippy AM, Konstantinidis KT, Aluru S High throughput ANI analysis of 90K prokaryotic genomes reveals clear species boundaries. Nat Commun 2018 30;9:5114. https:\/\/doi.org\/10.1038\/s41467-018-07641-9","DOI":"10.1038\/s41467-018-07641-9"},{"key":"4910_CR20","unstructured":"A genomic surveillance framework and genotyping tool for Klebsiella\u00a0pneumoniae and its related species complex - PubMed [Internet] [cited 2024 22]. doi: https:\/\/pubmed.ncbi.nlm.nih.gov\/34234121\/"},{"key":"4910_CR21","doi-asserted-by":"publisher","first-page":"2640","DOI":"10.1093\/jac\/dks261","volume":"67","author":"E Zankari","year":"2012","unstructured":"Zankari E, Hasman H, Cosentino S, Vestergaard M, Rasmussen S, Lund O et al (2012) Identification of acquired antimicrobial resistance genes. J Antimicrob Chemother 67:2640\u20132644. https:\/\/doi.org\/10.1093\/jac\/dks261","journal-title":"J Antimicrob Chemother"},{"key":"4910_CR22","doi-asserted-by":"publisher","first-page":"740","DOI":"10.1017\/ice.2020.68","volume":"41","author":"Y Liu","year":"2020","unstructured":"Liu Y, Zhang X, Cai L, Zong Z (2020) Enhanced survival of ST-11 carbapenem-resistant Klebsiella pneumoniae in the intensive care unit. Infect Control Hosp Epidemiol 41:740\u2013742. https:\/\/doi.org\/10.1017\/ice.2020.68","journal-title":"Infect Control Hosp Epidemiol"},{"key":"4910_CR23","volume-title":"Molecular cloning: a Laboratory Manual","author":"J Sambrook","year":"2001","unstructured":"Sambrook J, Russell D (2001) Molecular cloning: a Laboratory Manual, 3rd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, (NY)","edition":"3"},{"key":"4910_CR24","doi-asserted-by":"publisher","unstructured":"Shields RK, Nguyen MH, Press EG, Chen L, Kreiswirth BN, Clancy CJ (2017) In Vitro Selection of Meropenem Resistance among Ceftazidime-Avibactam-Resistant, Meropenem-Susceptible Klebsiella pneumoniae isolates with variant KPC-3 carbapenemases. Antimicrob Agents Chemother 61. https:\/\/doi.org\/10.1128\/AAC.00079-17","DOI":"10.1128\/AAC.00079-17"},{"key":"4910_CR25","doi-asserted-by":"publisher","unstructured":"Liu S, Jing L, Yu Z-J, Wu C, Zheng Y, Zhang E et al ((S)-3-Mercapto-2-methylpropanamido)acetic acid derivatives as metallo-\u03b2-lactamase inhibitors: synthesis, kinetic and crystallographic studies. Eur J Med Chem 2018 10;145:649\u2013660. https:\/\/doi.org\/10.1016\/j.ejmech.2018.01.032","DOI":"10.1016\/j.ejmech.2018.01.032"},{"key":"4910_CR26","doi-asserted-by":"publisher","unstructured":"Wu S, Feng Y, Yang Y, Zhu K, Wang S, Wang X et al (2023) Characterization of a novel carbapenem-hydrolysing \u03b2-lactamase OXA-1041 in Escherichia coli. J Antimicrob Chemother 78:1288\u20131294. https:\/\/doi.org\/10.1093\/jac\/dkad091","DOI":"10.1093\/jac\/dkad091"},{"key":"4910_CR27","doi-asserted-by":"publisher","first-page":"3895","DOI":"10.1128\/aac.02412-14","volume":"58","author":"A Carattoli","year":"2014","unstructured":"Carattoli A, Zankari E, Garc\u00eda-Fern\u00e1ndez A, Voldby Larsen M, Lund O, Villa L et al (2014) In silico detection and typing of plasmids using PlasmidFinder and plasmid multilocus sequence typing. Antimicrob Agents Chemother 58:3895\u20133903. https:\/\/doi.org\/10.1128\/aac.02412-14","journal-title":"Antimicrob Agents Chemother"},{"key":"4910_CR28","doi-asserted-by":"publisher","first-page":"1239","DOI":"10.3390\/genes11111239","volume":"22","author":"C Virolle","year":"2020","unstructured":"Virolle C, Goldlust K, Djermoun S, Bigot S, Lesterlin C (2020) Plasmid transfer by conjugation in Gram-negative Bacteria: from the Cellular to the Community Level. Genes (Basel) 22:11:1239. https:\/\/doi.org\/10.3390\/genes11111239","journal-title":"Genes (Basel)"},{"key":"4910_CR29","doi-asserted-by":"publisher","first-page":"500","DOI":"10.1128\/AAC.46.2.500-510.2002","volume":"46","author":"TM Coque","year":"2002","unstructured":"Coque TM, Oliver A, P\u00e9rez-D\u00edaz JC, Baquero F, Cant\u00f3n R (2002) Genes encoding TEM-4, SHV-2, and CTX-M-10 extended-spectrum beta-lactamases are carried by multiple Klebsiella pneumoniae clones in a single hospital (Madrid, 1989 to 2000). Antimicrob Agents Chemother 46:500\u2013510. https:\/\/doi.org\/10.1128\/AAC.46.2.500-510.2002","journal-title":"Antimicrob Agents Chemother"},{"key":"4910_CR30","doi-asserted-by":"publisher","unstructured":"Wyres KL, Wick RR, Gorrie C, Jenney A, Follador R, Thomson NR et al (2016) Identification of Klebsiella capsule synthesis loci from whole genome data. Microbial Genomics [Internet]. [cited 2023 11]:2. doi: https:\/\/www.microbiologyresearch.org\/content\/journal\/mgen\/https:\/\/doi.org\/10.1099\/mgen.0.000102","DOI":"10.1099\/mgen.0.000102"},{"key":"4910_CR31","doi-asserted-by":"publisher","first-page":"e1007218","DOI":"10.1371\/journal.ppat.1007218","volume":"15","author":"A Fajardo-Lubi\u00e1n","year":"2019","unstructured":"Fajardo-Lubi\u00e1n A, Ben Zakour NL, Agyekum A, Qi Q, Iredell JR (2019) Host adaptation and convergent evolution increases antibiotic resistance without loss of virulence in a major human pathogen. PLoS Pathog 15:e1007218. https:\/\/doi.org\/10.1371\/journal.ppat.1007218","journal-title":"PLoS Pathog"},{"key":"4910_CR32","doi-asserted-by":"publisher","first-page":"W320","DOI":"10.1093\/nar\/gku316","volume":"42","author":"X Robert","year":"2014","unstructured":"Robert X, Gouet P (2014) Deciphering key features in protein structures with the new ENDscript server. Nucleic Acids Res 42:W320\u2013324. https:\/\/doi.org\/10.1093\/nar\/gku316","journal-title":"Nucleic Acids Res"},{"key":"4910_CR33","doi-asserted-by":"publisher","unstructured":"Alikhan N-F, Petty NK, Ben Zakour NL, Beatson SA BLAST Ring Image Generator (BRIG): simple prokaryote genome comparisons. BMC Genomics 2011 8;12:402. https:\/\/doi.org\/10.1186\/1471-2164-12-402","DOI":"10.1186\/1471-2164-12-402"}],"container-title":["European Journal of Clinical Microbiology &amp; Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10096-024-04910-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10096-024-04910-y\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10096-024-04910-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,16]],"date-time":"2024-09-16T16:23:01Z","timestamp":1726503781000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10096-024-04910-y"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,7]]},"references-count":33,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2024,10]]}},"alternative-id":["4910"],"URL":"https:\/\/doi.org\/10.1007\/s10096-024-04910-y","relation":{},"ISSN":["0934-9723","1435-4373"],"issn-type":[{"type":"print","value":"0934-9723"},{"type":"electronic","value":"1435-4373"}],"subject":[],"published":{"date-parts":[[2024,8,7]]},"assertion":[{"value":"14 March 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"24 July 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"7 August 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"This work was approved by the West China Hospital Ethics Committee(2020\u2009\u2212\u2009739).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}},{"value":"Xiaoju Lv and Zhiyong Zong designed the study. Yanqiao Gong performed the experiments. Yanqiao Gong and Yu Feng analyzed the data. Yanqiao Gong and Xiaoju Lv wrote the manuscript. All authors reviewed the manuscript.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to participate"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}