{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T02:10:42Z","timestamp":1778119842304,"version":"3.51.4"},"reference-count":30,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,11,9]],"date-time":"2024-11-09T00:00:00Z","timestamp":1731110400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,11,9]],"date-time":"2024-11-09T00:00:00Z","timestamp":1731110400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100006389","name":"University of Geneva","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100006389","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Eur J Clin Microbiol Infect Dis"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:p>There is a constant need to reduce turn-around times and keep costs as low as possible for the carriage screening of GBS in pregnant patients. Laboratory automation might provide an edge in this field. The objectives of the present study were: i) to compare the performance of the direct chromID\u2122 Strepto B agar (CA) plating against LIM-broth enriched plating on CA for the detection of GBS from vagino-rectal screening-swabs; and ii) to assess the usage of PhenoMATRIX\u2122 for the automated screening of GBS. Between January 2021 and December 2023, 9\u2032107 vagino-rectal specimens were collected from pregnant women at Geneva University Hospitals and were used to address the first objective. There was a small difference in the GBS detection rates between direct CA plating (13.2%; 1\u2032202\/9\u2032107) and LIM-broth enriched plating on CA (13.2%; 1\u2032198\/9\u2032107). Based on the LIM-broth enrichment results, the sensitivity and specificity of the direct CA plating were 98.3% (95% CI, 97.3%\u201498.9%) and 99.7% (95% CI, 99.5%\u201499.8%), respectively. Importantly, for 25 specimens, GBS growth was only detected by direct CA plating. We used a random set of 8\u2032768 CA\u00a0plate pictures for the machine learning of PhenoMATRIX\u2122. The validation was carried out on an additional set of 830 CA\u00a0plate pictures. The sensitivity and specificity of PhenoMATRIX\u2122 were 100% (95% CI, 96.6%\u2014100.0%) and 90.2% (95% CI, 87.8%\u201492.1%), respectively. We established that for GBS screening, the performance of direct CA plating is not inferior to the LIM-broth enriched approach. By relying on PhenoMATRIX\u2122, the negative predictive value for GBS screening reaches 100% (95% CI, 99.4%\u2014100.0%), enabling the automatic release of GBS-negative cases within 24\u00a0h.<\/jats:p>","DOI":"10.1007\/s10096-024-04985-7","type":"journal-article","created":{"date-parts":[[2024,11,9]],"date-time":"2024-11-09T08:08:30Z","timestamp":1731139710000},"page":"63-69","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["PhenoMATRIX\u2122 for the screening of Group B Streptococcus (GBS) carriage in pregnant women: ready to get rid of the LIM broth?"],"prefix":"10.1007","volume":"44","author":[{"given":"Abdessalam","family":"Cherkaoui","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gesuele","family":"Renzi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacques","family":"Schrenzel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,11,9]]},"reference":[{"key":"4985_CR1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3310\/hta11290","volume":"11","author":"T Colbourn","year":"2007","unstructured":"Colbourn T, Asseburg C, Bojke L et al (2007) Prenatal screening and treatment strategies to prevent group B streptococcal and other bacterial infections in early infancy: Cost-effectiveness and expected value of information analyses. 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Thus, non informed consent is required. The official name of the ethics committee is \u201cCommission cantonale d\u2019\u00e9thique de la recherche (CCER)\u201d All experimental protocols were approved\u00a0by the ethics committee.All methods were carried out in accordance with relevant guidelines and regulations.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Informed consent"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"This research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest or competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflicts of interest\/Competing interests"}}]}}