{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T20:25:45Z","timestamp":1785356745910,"version":"3.55.0"},"reference-count":33,"publisher":"Springer Science and Business Media LLC","issue":"3","license":[{"start":{"date-parts":[[2025,1,14]],"date-time":"2025-01-14T00:00:00Z","timestamp":1736812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,1,14]],"date-time":"2025-01-14T00:00:00Z","timestamp":1736812800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Eur J Clin Microbiol Infect Dis"],"published-print":{"date-parts":[[2025,3]]},"DOI":"10.1007\/s10096-025-05037-4","type":"journal-article","created":{"date-parts":[[2025,1,14]],"date-time":"2025-01-14T16:06:48Z","timestamp":1736870808000},"page":"717-728","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["High detection rate of parasitic load by qPCR targeting 18S rDNA in blood of patients with active leishmaniasis lesions"],"prefix":"10.1007","volume":"44","author":[{"given":"Gabriel Victor","family":"Castanheira","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valdir Sabbaga","family":"Amato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felipe Francisco","family":"Tuon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Let\u00edcia Ramos","family":"Dantas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvio Fernando Guimar\u00e3es","family":"de Carvalho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thallyta Maria","family":"Vieira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Paula Venuto","family":"Moura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jackeline Maria","family":"de Sousa Lima Lopes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo Henrique","family":"Johanson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christini Takemi","family":"Emori","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Regina Maia","family":"de Souza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,1,14]]},"reference":[{"issue":"9","key":"5037_CR1","doi-asserted-by":"publisher","first-page":"581","DOI":"10.1016\/S1473-3099(07)70209-8","volume":"7","author":"R Reithinger","year":"2007","unstructured":"Reithinger R, Dujardin JC, Louzir H, Pirmez C, Alexander B, Brooker S (2007) Cutaneous leishmaniasis. Lancet Infect Dis 7(9):581\u2013596","journal-title":"Lancet Infect Dis"},{"issue":"1","key":"5037_CR2","doi-asserted-by":"publisher","first-page":"81","DOI":"10.4269\/ajtmh.13-0578","volume":"91","author":"FF Tuon","year":"2014","unstructured":"Tuon FF, Bombonatto GM, Battaglin ER, Sakumoto MH, Amato VS, de Camargo RA, Nicodemo AC (2014) Reactivation of mucosal and cutaneous leishmaniasis in a renal transplanted patient. Am J Trop Med Hyg 91(1):81\u201383","journal-title":"Am J Trop Med Hyg"},{"issue":"11","key":"5037_CR3","doi-asserted-by":"publisher","first-page":"981","DOI":"10.1093\/trstmh\/trac047","volume":"116","author":"FF Tuon","year":"2022","unstructured":"Tuon FF, Amato VS, Zequinao T, Cruz JAW (2022) Emerging computational technologies in human leishmaniasis: where are we? Trans R Soc Trop Med Hyg 116(11):981\u2013985","journal-title":"Trans R Soc Trop Med Hyg"},{"issue":"10","key":"5037_CR4","doi-asserted-by":"publisher","first-page":"258","DOI":"10.3390\/tropicalmed7100258","volume":"7","author":"E Favi","year":"2022","unstructured":"Favi E, Santolamazza G, Botticelli F, Alfieri C, Delbue S, Cacciola R, Guarneri A, Ferraresso M (2022) Epidemiology, Clinical Characteristics, Diagnostic Work Up, and Treatment Options of Leishmania Infection in Kidney Transplant Recipients: A Systematic Review. Trop Med Infect Dis 7(10):258","journal-title":"Trop Med Infect Dis"},{"issue":"3","key":"5037_CR5","doi-asserted-by":"publisher","first-page":"234","DOI":"10.1016\/j.exppara.2011.08.010","volume":"129","author":"DR Bezerra-Vasconcelos","year":"2011","unstructured":"Bezerra-Vasconcelos DR, Melo LM, Albuquerque ES, Luciano MC, Bevilaqua CM (2011) Real-time PCR to assess the Leishmania load in Lutzomyia longipalpis sand flies: screening of target genes and assessment of quantitative methods. Exp Parasitol 129(3):234\u2013239","journal-title":"Exp Parasitol"},{"issue":"14","key":"5037_CR6","doi-asserted-by":"publisher","first-page":"1891","DOI":"10.1017\/S0031182014001280","volume":"141","author":"ER Adams","year":"2014","unstructured":"Adams ER, Gomez MA, Scheske L, Rios R, Marquez R, Cossio A, Albertini A, Schallig H, Saravia NG (2014) Sensitive diagnosis of cutaneous leishmaniasis by lesion swab sampling coupled to qPCR. Parasitology 141(14):1891\u20131897","journal-title":"Parasitology"},{"key":"5037_CR7","first-page":"8","volume":"2017","author":"CM Leon","year":"1907","unstructured":"Leon CM, Munoz M, Hernandez C, Ayala MS, Florez C, Teheran A, Cubides JR, Ramirez JD (1907) Analytical performance of four polymerase chain reaction (PCR) and real time PCR (qPCR) assays for the detection of six leishmania species DNA in Colombia. Front Microbiol 2017:8","journal-title":"Front Microbiol"},{"issue":"1","key":"5037_CR8","doi-asserted-by":"publisher","first-page":"273","DOI":"10.1186\/s13071-018-2859-8","volume":"11","author":"L Galluzzi","year":"2018","unstructured":"Galluzzi L, Ceccarelli M, Diotallevi A, Menotta M, Magnani M (2018) Real-time PCR applications for diagnosis of leishmaniasis. Parasit Vectors 11(1):273","journal-title":"Parasit Vectors"},{"issue":"5","key":"5037_CR9","doi-asserted-by":"publisher","first-page":"701","DOI":"10.1590\/abd1806-4841.20142389","volume":"89","author":"CM Gomes","year":"2014","unstructured":"Gomes CM, Paula NA, Morais OO, Soares KA, Roselino AM, Sampaio RN (2014) Complementary exams in the diagnosis of American tegumentary leishmaniasis. An Bras Dermatol 89(5):701\u2013709","journal-title":"An Bras Dermatol"},{"issue":"1","key":"5037_CR10","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1016\/j.actatropica.2003.10.008","volume":"90","author":"AC Volpini","year":"2004","unstructured":"Volpini AC, Passos VM, Oliveira GC, Romanha AJ (2004) PCR-RFLP to identify Leishmania (Viannia) braziliensis and L. (Leishmania) amazonensis causing American cutaneous leishmaniasis. Acta Trop 90(1):31\u201337","journal-title":"Acta Trop"},{"issue":"10","key":"5037_CR11","doi-asserted-by":"publisher","first-page":"e0008750","DOI":"10.1371\/journal.pntd.0008750","volume":"14","author":"CPB Filgueira","year":"2020","unstructured":"Filgueira CPB, Moreira OC, Cantanhede LM, de Farias HMT, Porrozzi R, Britto C, Boite MC, Cupolillo E (2020) Comparison and clinical validation of qPCR assays targeting Leishmania 18S rDNA and HSP70 genes in patients with American Tegumentary Leishmaniasis. PLoS Negl Trop Dis 14(10):e0008750","journal-title":"PLoS Negl Trop Dis"},{"issue":"11","key":"5037_CR12","doi-asserted-by":"publisher","first-page":"3892","DOI":"10.1128\/JCM.r00764-11","volume":"49","author":"JL Weirather","year":"2011","unstructured":"Weirather JL, Jeronimo SM, Gautam S, Sundar S, Kang M, Kurtz MA, Haque R, Schriefer A, Talhari S, Carvalho EM et al (2011) Serial quantitative PCR assay for detection, species discrimination, and quantification of Leishmania spp. in human samples. J Clin Microbiol 49(11):3892\u20133904","journal-title":"J Clin Microbiol"},{"key":"5037_CR13","doi-asserted-by":"publisher","first-page":"101791","DOI":"10.1016\/j.mcp.2022.101791","volume":"61","author":"RM de Souza","year":"2022","unstructured":"de Souza RM, Martins RC, Franco LA, Tuon FF, de Oliveira Junior IG, da Silva CA, Imamura R, Amato VS (2022) Identification of Leishmania species by next generation sequencing of hsp70 gene. Mol Cell Probes. 61:101791","journal-title":"Mol Cell Probes."},{"issue":"2","key":"5037_CR14","doi-asserted-by":"publisher","first-page":"e0004422","DOI":"10.1371\/journal.pntd.0004422","volume":"10","author":"P Bhattacharya","year":"2016","unstructured":"Bhattacharya P, Ghosh S, Ejazi SA, Rahaman M, Pandey K, Ravi Das VN, Das P, Goswami RP, Saha B, Ali N (2016) Induction of IL-10 and TGFbeta from CD4+CD25+FoxP3+ T cells correlates with parasite load in Indian Kala-azar patients infected with leishmania donovani. PLoS Negl Trop Dis 10(2):e0004422","journal-title":"PLoS Negl Trop Dis"},{"issue":"4","key":"5037_CR15","doi-asserted-by":"publisher","first-page":"611","DOI":"10.1373\/clinchem.2008.112797","volume":"55","author":"SA Bustin","year":"2009","unstructured":"Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, Kubista M, Mueller R, Nolan T, Pfaffl MW, Shipley GL et al (2009) The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem 55(4):611\u2013622","journal-title":"Clin Chem"},{"issue":"22","key":"5037_CR16","doi-asserted-by":"publisher","first-page":"5259","DOI":"10.3390\/molecules29225259","volume":"29","author":"NT Capistrano Costa","year":"2024","unstructured":"Capistrano Costa NT, de Souza Pereira AM, Silva CC, Souza ED, de Oliveira BC, Ferreira LF, Hernandes MZ, Pereira VR (2024) Exploring bioinformatics solutions for improved leishmaniasis diagnostic tools: a review. Molecules. 29(22):5259","journal-title":"Molecules."},{"issue":"4","key":"5037_CR17","doi-asserted-by":"publisher","first-page":"352","DOI":"10.1177\/00494755241273086","volume":"54","author":"A Sarfraz","year":"2024","unstructured":"Sarfraz A, Sarfraz Z, Liaqat M, Khan MH, Abdul Jabbar HA, Abdullah M (2024) Leishmaniasis - Still a diagnostic challenge: An individual participant data systematic review. Trop Doct 54(4):352\u2013358","journal-title":"Trop Doct"},{"issue":"1","key":"5037_CR18","doi-asserted-by":"publisher","first-page":"349","DOI":"10.1016\/S0732-8893(03)00093-2","volume":"47","author":"G Schonian","year":"2003","unstructured":"Schonian G, Nasereddin A, Dinse N, Schweynoch C, Schallig HD, Presber W, Jaffe CL (2003) PCR diagnosis and characterization of Leishmania in local and imported clinical samples. Diagn Microbiol Infect Dis 47(1):349\u2013358","journal-title":"Diagn Microbiol Infect Dis"},{"issue":"4","key":"5037_CR19","doi-asserted-by":"publisher","first-page":"372","DOI":"10.4103\/0972-9062.374236","volume":"60","author":"M Sarmadi","year":"2023","unstructured":"Sarmadi M, Bagherian Z, Ahmadi-Soleimani SM, Rezaiemanesh MR, Khodamoradi F, Rahimi S, Azizi H (2023) Environmental health risk factors and cutaneous leishmaniasis (CL): A case-control study in northeastern Iran. J Vector Borne Dis 60(4):372\u2013381","journal-title":"J Vector Borne Dis"},{"issue":"4","key":"5037_CR20","doi-asserted-by":"publisher","first-page":"523","DOI":"10.7162\/S1809-97772012000400015","volume":"16","author":"BT Moraes","year":"2012","unstructured":"Moraes BT, FilhoFde S, Neto JC, Neto PS, Melo JEJ (2012) Laryngeal leishmaniasis. Int Arch Otorhinolaryngol 16(4):523\u2013526","journal-title":"Int Arch Otorhinolaryngol"},{"issue":"4","key":"5037_CR21","doi-asserted-by":"publisher","first-page":"785","DOI":"10.4269\/ajtmh.22-0180","volume":"107","author":"VS Amato","year":"2022","unstructured":"Amato VS, de Souza RM, Franco LA, Martins RC, da Silva CA, Emori CT, Celeste BJ, Castanheira GV, Tuon FF (2022) Case Report: Cutaneous Leishmaniasis in a Rheumatoid Arthritis Patient Receiving Methotrexate. Am J Trop Med Hyg 107(4):785","journal-title":"Am J Trop Med Hyg"},{"key":"5037_CR22","doi-asserted-by":"publisher","first-page":"e38","DOI":"10.1590\/s1678-9946201759038","volume":"59","author":"SM Boni","year":"2017","unstructured":"Boni SM, Oyafuso LK, Soler RC, Lindoso JAL (2017) Efficiency of noninvasive sampling methods (swab) together with polymerase chain reaction (PCR) for diagnosing American tegumentary leishmaniasis. Rev Inst Med Trop Sao Paulo 59:e38","journal-title":"Rev Inst Med Trop Sao Paulo"},{"key":"5037_CR23","doi-asserted-by":"publisher","first-page":"106787","DOI":"10.1016\/j.actatropica.2022.106787","volume":"238","author":"RL Ursine","year":"2023","unstructured":"Ursine RL, Rocha MF, Neto FC, Leite ME, Dolabela Falcao L, Gorla DE, de Carvalho SFG, Vieira TM (2023) Influence of anthropic changes and environmental characteristics on the occurrence of Tegumentary Leishmaniasis in Montes Claros, Minas Gerais, Brazil, between 2012 and 2019. Acta Trop 238:106787","journal-title":"Acta Trop"},{"issue":"5","key":"5037_CR24","doi-asserted-by":"publisher","first-page":"996","DOI":"10.4269\/ajtmh.21-1120","volume":"107","author":"M Solomon","year":"2022","unstructured":"Solomon M, Gimple A, Fuchs I, Cicurel A, Meninger T, Avni D, Nasereddin A, Jaffe CL, Schwartz E (2022) Failure to detect leishmania in the blood of patients with old-world cutaneous leishmaniasis: Implications for blood donation. Am J Trop Med Hyg 107(5):996\u2013998","journal-title":"Am J Trop Med Hyg"},{"issue":"4","key":"5037_CR25","doi-asserted-by":"publisher","first-page":"399","DOI":"10.1016\/j.exppara.2006.10.002","volume":"115","author":"EA Venazzi","year":"2007","unstructured":"Venazzi EA, Roberto AC, Barbosa-Tessmann IP, Zanzarini PD, Lonardoni MV, Silveira TG (2007) Detection of Leishmania (Viannia) DNA in blood from patients with American cutaneous leishmaniasis. Exp Parasitol 115(4):399\u2013402","journal-title":"Exp Parasitol"},{"issue":"12","key":"5037_CR26","doi-asserted-by":"publisher","first-page":"1112","DOI":"10.1016\/j.trstmh.2006.02.014","volume":"100","author":"PDO Camera","year":"2006","unstructured":"Camera PDO, Junger J, Pires FDESS, Mattos M, Oliveira-Neto MP, Fernandes O, Pirmez C (2006) Haematogenous dissemination of Leishmania (Viannia) braziliensis in human American tegumentary leishmaniasis. Trans R Soc Trop Med Hyg 100(12):1112\u20131117","journal-title":"Trans R Soc Trop Med Hyg"},{"issue":"7","key":"5037_CR27","doi-asserted-by":"publisher","first-page":"e491","DOI":"10.1016\/j.ijid.2011.03.022","volume":"15","author":"H Nakkash-Chmaisse","year":"2011","unstructured":"Nakkash-Chmaisse H, Makki R, Nahhas G, Knio K, Nuwayri-Salti N (2011) Detection of Leishmania parasites in the blood of patients with isolated cutaneous leishmaniasis. Int J Infect Dis 15(7):e491-494","journal-title":"Int J Infect Dis"},{"issue":"3","key":"5037_CR28","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1590\/S0037-86821989000300006","volume":"22","author":"FT Silveira","year":"1989","unstructured":"Silveira FT, Lainson R, Shaw JJ, Ishikawa EA, Souza AA, Braga RR (1989) Sensitivity of the culture of circulating leukocytes in the detection of Leishmania in the peripheral blood of patients with tegumentary leishmaniasis. Rev Soc Bras Med Trop 22(3):143\u2013146","journal-title":"Rev Soc Bras Med Trop"},{"key":"5037_CR29","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1016\/j.actatropica.2017.09.020","volume":"184","author":"OC Moreira","year":"2018","unstructured":"Moreira OC, Yadon ZE, Cupolillo E (2018) The applicability of real-time PCR in the diagnostic of cutaneous leishmaniasis and parasite quantification for clinical management: Current status and perspectives. Acta Trop 184:29\u201337","journal-title":"Acta Trop"},{"key":"5037_CR30","doi-asserted-by":"publisher","first-page":"e6","DOI":"10.1590\/s1678-9946201759006","volume":"59","author":"RM Souza","year":"2017","unstructured":"Souza RM, Andrade HFJ, Duarte MI, Braz LM, Schubach AO, Silva FC, Amato VS (2017) Reactivation of cutaneous and mucocutaneous tegumentary leishmaniasis in rheumatoid arthritis patients: an emerging problem? Rev Inst Med Trop Sao Paulo 59:e6","journal-title":"Rev Inst Med Trop Sao Paulo"},{"issue":"2","key":"5037_CR31","doi-asserted-by":"publisher","first-page":"228","DOI":"10.4269\/ajtmh.23-0287","volume":"110","author":"VS Amato","year":"2024","unstructured":"Amato VS, Franco LA, de Souza RM, da Silva CA, Ramundo MS, C\u00f4rtes MF, Corr\u00eaa-Castro G, Santos Oliveira JR, Da Cruz AM, Pagliari C, Galo LK (2024) Case Report: In Situ and Systemic Immune Response to Mucosal Leishmaniasis in an HIV-Infected Patient. Am J Trop Med Hyg 110(2):228\u201333","journal-title":"Am J Trop Med Hyg"},{"issue":"Suppl 1","key":"5037_CR32","first-page":"S45","volume":"56","author":"T Jimenez-Marco","year":"2016","unstructured":"Jimenez-Marco T, Fisa R, Girona-Llobera E, Cancino-Faure B, Tomas-Perez M, Berenguer D, Guillen C, Pujol A, Iniesta L, Serra T et al (2016) Transfusion-transmitted leishmaniasis: a practical review. Transfusion 56(Suppl 1):S45-51","journal-title":"Transfusion"},{"issue":"4","key":"5037_CR33","first-page":"804","volume":"111","author":"P Piyaraj","year":"2024","unstructured":"Piyaraj P, Bualert L, Kalrat A, Leelayoova S, Ruang-Areerate T, Theprin N, Naaglor T, Mungthin M (2024) Asymptomatic Leishmania Infection among Blood Donors in a Southern Province of Thailand. Am J Trop Med Hyg 111(4):804\u2013813","journal-title":"Am J Trop Med Hyg"}],"container-title":["European Journal of Clinical Microbiology & Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10096-025-05037-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10096-025-05037-4\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10096-025-05037-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,4]],"date-time":"2025-03-04T14:22:50Z","timestamp":1741098170000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10096-025-05037-4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,14]]},"references-count":33,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,3]]}},"alternative-id":["5037"],"URL":"https:\/\/doi.org\/10.1007\/s10096-025-05037-4","relation":{},"ISSN":["0934-9723","1435-4373"],"issn-type":[{"value":"0934-9723","type":"print"},{"value":"1435-4373","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,14]]},"assertion":[{"value":"15 October 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"8 January 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 January 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"This project was approved by the Research Ethics Committee (CAPPesq) of the Faculty of Medicine of the University of S\u00e3o Paulo, identified by CAAE n\u00ba 56014121.0.0000.0068. The Free and Informed Consent Terms were applied and obtained during outpatient care at the coordinating center of the University of S\u00e3o Paulo and at the co-participating center of the State University of Montes Claros.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"The authors declare that they have no known competing financial interests or personal relationships that could have influenced the work reported in this paper.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest disclosure"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"Not applicable.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Clinical trial number"}}]}}