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Eazyplex<jats:sup>\u00ae<\/jats:sup> MRSA Plus loop\u2010mediated isothermal amplification (LAMP) assay directly from BC fluid; b. MALDI-TOF MS subtyping on BC pellet extracted with Rapid Sepsityper<jats:sup>\u00ae<\/jats:sup> protocol and on 4-h short-term subculture; c. Clearview\u2122 Culture Colony\u00a0PBP2a SA immunochromatography assay on BC pellet and on 4-h short-term subculture; d. EUCAST RAST cefoxitin screen test performed directly from BC and including reading times at 4-h, 6-h and 16\u201320-h.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Eazyplex<jats:sup>\u00ae<\/jats:sup> MRSA plus exhibited the best performance, showing 100% sensitivity, specificity, positive predictive value, and negative predictive value, followed by PBP2a SA Culture Colony Clearview assay and EUCAST RAST cefoxitin screen. MALDI-TOF MS subtyping showed the lowest diagnostic accuracy (59.8 and 65.7% directly from BC and from 4-h subculture, respectively). In detail, sensitivity and specificity ranged from 24.3% to 20.4% and from 88.9% to 98.3% for protocols performed from BC pellet and 4-h subculture, respectively.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>The Eazyplex<jats:sup>\u00ae<\/jats:sup> MRSA Plus and the immunochromatographic Clearview\u2122 PBP2a SA Culture Colony methods can provide reliable results within 1\u00a0h from the start of positive BC processing. MALDI TOF MS subtyping showed unacceptable specificity by performing analysis from BC pellets, while its sensitivity depends on the prevalence of PSM-positive MRSA strains. The EUCAST RAST, based on disc diffusion, showed excellent performance with a time-to-result of at least 4\u00a0h.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1007\/s10096-025-05039-2","type":"journal-article","created":{"date-parts":[[2025,1,22]],"date-time":"2025-01-22T02:51:33Z","timestamp":1737514293000},"page":"827-837","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Diagnostic methods and protocols for rapid determination of methicillin resistance in Staphylococcus aureus bloodstream infections: a comparative analysis"],"prefix":"10.1007","volume":"44","author":[{"given":"Matteo","family":"Boattini","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luisa","family":"Guarrasi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Comini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guido","family":"Ricciardelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto","family":"Casale","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rossana","family":"Cavallo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Costa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriele","family":"Bianco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,1,22]]},"reference":[{"issue":"4","key":"5039_CR1","doi-asserted-by":"publisher","first-page":"e00020","DOI":"10.1128\/CMR.00020-18","volume":"31","author":"S Lakhundi","year":"2018","unstructured":"Lakhundi S, Zhang K (2018) Methicillin-Resistant Staphylococcus aureus: Molecular Characterization, Evolution, and Epidemiology. 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