{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:19:17Z","timestamp":1788221957686,"version":"build-2803163510"},"reference-count":30,"publisher":"Springer Science and Business Media LLC","issue":"6","license":[{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001734","name":"Copenhagen University","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001734","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Eur J Clin Microbiol Infect Dis"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>We aimed to describe the genomic epidemiology of vancomycin-resistant <jats:italic>Enterococcus faecium<\/jats:italic> (VREfm) in Eastern Denmark from 2020 to 2022, identify and characterise the <jats:italic>vanB<\/jats:italic> Transposon <jats:italic>1549<\/jats:italic> (Tn<jats:italic>1549<\/jats:italic>) insertion sites among <jats:italic>vanB<\/jats:italic> VREfm clones and identify emerging VREfm clones.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>We analysed all VREfm from our routine diagnostic sequencing during the study period. Using the Seqsphere\u2009+\u2009v.8.2.0 software (Ridom GmbH, M\u00fcnster, Germany, (<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.ridom.de\/seqsphere\" ext-link-type=\"uri\">http:\/\/www.ridom.de\/seqsphere<\/jats:ext-link>), minimum spanning trees were created to visualise clusters. Tn<jats:italic>1549<\/jats:italic> insertion sites were determined by in silico PCR. Nanopore sequencing was performed to assemble the regions surrounding Tn<jats:italic>1549<\/jats:italic>, which helped determine the insertion site locations.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>We included 2,437 isolates in the study. A total of 463 isolates carried <jats:italic>vanA<\/jats:italic>, 1,963 isolates carried <jats:italic>vanB<\/jats:italic>, and 11 isolates carried both genes. Of all isolates carrying <jats:italic>vanB<\/jats:italic>, 254 isolates had the Tn<jats:italic>1549<\/jats:italic> inserted in the <jats:italic>araA2<\/jats:italic> gene, 1,604 in the <jats:italic>sir2<\/jats:italic> gene, and 116 in neither the <jats:italic>araA2<\/jats:italic> nor <jats:italic>sir2<\/jats:italic> genes. We identified eight chromosomal insertion sites other than in the <jats:italic>araA2<\/jats:italic> and <jats:italic>sir2<\/jats:italic> genes. Three isolates carried the Tn<jats:italic>1549<\/jats:italic> on plasmids. No emerging clones were found.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>We have described the genomic epidemiology during the study period and identified ten chromosomal Tn<jats:italic>1549<\/jats:italic> insertion sites.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1007\/s10096-025-05091-y","type":"journal-article","created":{"date-parts":[[2025,4,3]],"date-time":"2025-04-03T19:26:32Z","timestamp":1743708392000},"page":"1425-1432","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Genomic epidemiology of vancomycin-resistant Enterococcus faecium in Eastern Denmark from 2020 to 2022, and identification of vanB Tn1549 insertion sites"],"prefix":"10.1007","volume":"44","author":[{"given":"Maja Johanne S\u00f8ndergaard","family":"Knudsen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose Alfredo Samaniego","family":"Castruita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingrid Maria Cecilia","family":"Rubin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Mollerup","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helle Krogh","family":"Johansen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rasmus L.","family":"Marvig","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen Leth","family":"Nielsen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara Juliane","family":"Holzknecht","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Morten","family":"Hoppe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Kemp","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henrik","family":"Westh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mette","family":"Pinholt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,4,1]]},"reference":[{"key":"5091_CR1","doi-asserted-by":"publisher","unstructured":"Bager P et al (Dec. 2024) Comparison of morbidity and mortality after bloodstream infection with vancomycin-resistant versus -susceptible Enterococcus faecium: a nationwide cohort study in Denmark, 2010\u20132019. 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The study was performed in accordance with the Declaration of Helsinki.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to participate"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}