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This study compared the diagnostic performance of two multiplex PCR panels\u2014 the <jats:bold>Seegene Allplex\u2122 Gastrointestinal Panels<\/jats:bold> (Seegene, Seoul, Korea) and the <jats:bold>Luminex NxTAG<\/jats:bold>\n              <jats:sup>\n                <jats:bold>\u00ae<\/jats:bold>\n              <\/jats:sup>\n              <jats:bold>Gastrointestinal Pathogen Panel<\/jats:bold> (Luminex Corporation, Austin, Texas, a Diasorin Company)\u2014in detecting gastrointestinal pathogens from clinical stool samples.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>A total of 196 stool samples, collected from patients at a Spanish hospital during 2023, were analyzed using both assays through prospective and retrospective approaches. The performance of each test was assessed based on Positive Percentage Agreement (PPA), Negative Percentage Agreement (NPA), and overall agreement. Discrepancies between methods were resolved with a third confirmatory technique when available.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Both assays demonstrated high overall concordance, with NPA values consistently above 95% and overall Kappa values exceeding 0.8 for most pathogens. The average PPA was greater than 89% for nearly all targets; however, lower agreement was observed for <jats:italic>Cryptosporidium<\/jats:italic> spp. (86.6%). Notably, discrepancies were primarily observed for certain pathogens, such as <jats:italic>Salmonella spp.<\/jats:italic> and <jats:italic>Cryptosporidium spp.<\/jats:italic>, highlighting the diagnostic challenges associated with these targets.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>To our knowledge, this is the first study to compare the novel Luminex NxTAG<jats:sup>\u00ae<\/jats:sup> panels from Diasorin with the Seegene Allplex\u2122. Both multiplex assays provide rapid and reliable detection of gastrointestinal pathogens, making them valuable tools in clinical diagnostics. Future research should focus on improving detection accuracy for challenging pathogens and expanding target panels to further enhance patient management and reduce disease burden.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1007\/s10096-025-05098-5","type":"journal-article","created":{"date-parts":[[2025,3,17]],"date-time":"2025-03-17T09:39:51Z","timestamp":1742204391000},"page":"1341-1348","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["A novel comparative evaluation of multiplex PCR panels for gastrointestinal pathogen detection: Seegene Allplex\u2122 vs. Luminex NxTAG\u00ae in clinical stool samples"],"prefix":"10.1007","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9360-7159","authenticated-orcid":false,"given":"Laura","family":"Seijas-Pereda","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana","family":"Mart\u00edn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raquel","family":"Menchero","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3745-6661","authenticated-orcid":false,"given":"Carlos","family":"Rescalvo-Casas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2838-7270","authenticated-orcid":false,"given":"Marcos","family":"Hernando-Gozalo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4098-4377","authenticated-orcid":false,"given":"Juan","family":"Cuadros-Gonz\u00e1lez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9816-3208","authenticated-orcid":false,"given":"Ram\u00f3n","family":"P\u00e9rez-Tanoira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,3,17]]},"reference":[{"key":"5098_CR1","unstructured":"Diarrhoeal disease https:\/\/www.who.int\/en\/news-room\/fact-sheets\/detail\/diarrhoeal-disease. 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However, the company had no influence on the study design, data analysis, interpretation of results, or the preparation of the manuscript.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}