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The evaluation of the limit of detection (LOD) of the APTIMA Combo 2\u2122 kit (Panther, Hologic) was performed on cryopreserved semen samples spiked with CT and NG at concentrations ranging from 1 to 106 IFU\/mL or CFU\/mL, respectively. The LOD was 102 IFU\/mL for CT and 10 CFU\/mL for NG in single infection or coinfection. An inhibitory effect of semen on amplification was highlighted. This study confirmed the performance of the APTIMA Combo 2\u2122 kit for screening cryopreserved sperm samples before donation.<\/jats:p>","DOI":"10.1007\/s10096-025-05138-0","type":"journal-article","created":{"date-parts":[[2025,4,30]],"date-time":"2025-04-30T06:31:09Z","timestamp":1745994669000},"page":"2007-2011","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Evaluation of the APTIMA Combo 2\u2122 kit for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae in frozen semen"],"prefix":"10.1007","volume":"44","author":[{"given":"Thibaut","family":"Lutz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robin","family":"Chautard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maxime","family":"Lafontaine","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Merve","family":"Genc","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arabella","family":"Touati","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sabine","family":"Pereyre","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olivia","family":"Peuchant","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C\u00e9cile","family":"B\u00e9b\u00e9ar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabien","family":"Garnier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S\u00e9bastien","family":"Hantz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,4,30]]},"reference":[{"key":"5138_CR1","unstructured":"Chlamydia [Internet] [cited 2024 7];doi: https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/chlamydia"},{"key":"5138_CR2","doi-asserted-by":"publisher","unstructured":"O\u2019Connell CM, Ferone ME Chlamydia trachomatis genital infections. 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