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Rapid screening and detection of these bacteria are essential to reduce sequelae and prevent transmission. This study evaluated the efficacy of the EasyNAT CT\/NG assay, which utilizes cross-priming amplification (CPA) technique for the rapid and simultaneous detection of CT and NG in diverse reproductive tract specimens, achieving diagnosis within 30\u00a0min.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>The clinical performance of the EasyNAT CT\/NG assay in detecting CT and NG was assessed using 198 clinical samples, with results compared to those of conventional in-house Real-Time PCR to determine concordance. Sensitivity was measured using serial dilutions of quantified plasmids and specificity was evaluated by incorporating DNA from 18 common STI pathogens. The assay\u2019s suitability as a point-of-care testing (POCT) tool was evaluated with the criteria outlined in Target Product Profiles (TPPs).<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>The EasyNAT CT\/NG assay demonstrated high concordance with Real-Time PCR, with rates of 98.5% for CT and 99.0% for NG. Concordance in urine samples reached 98.6% for CT and 100% for NG, while cervical swabs showed both 97.7% for CT and NG; vaginal and urethral swabs achieved 100% for both pathogens. Among the 198 samples, one urine specimen tested negative for CT by Real-Time PCR but positive by the EasyNAT CT\/NG assay, a positive result confirmed by the Cepheid Xpert CT\/NG assay. Two cervical swabs, negative for CT and NG by Real-Time PCR, yielded invalid results with the EasyNAT CT\/NG assay but were confirmed negative or CT and NG by the Cepheid Xpert CT\/NG assay. The EasyNAT CT\/NG assay reliably detected CT and NG in turbid specimens, though it may fail with severely hemolytic samples. Its detection limit was 400 copies\/mL, with no cross-reactivity observed across 18 other pathogens.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>The EasyNAT CT\/NG assay offers rapid, sensitive, and specific detection of CT and NG, proving valuable for infection screening and early diagnosis. It shows promise as a rapid POCT method.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1007\/s10096-025-05218-1","type":"journal-article","created":{"date-parts":[[2025,7,22]],"date-time":"2025-07-22T11:35:18Z","timestamp":1753184118000},"page":"2515-2525","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Rapid and simple detection of Chlamydia trachomatis and Neisseria gonorrhoeae using the EasyNAT CT\/NG assay based on cross-priming amplification"],"prefix":"10.1007","volume":"44","author":[{"given":"Qing","family":"Wu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongxiang","family":"Tu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingjie","family":"Dai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yumin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijuan","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,7,22]]},"reference":[{"key":"5218_CR1","doi-asserted-by":"publisher","first-page":"541","DOI":"10.1016\/S1473-3099(21)00448-5","volume":"22","author":"Y Zheng","year":"2022","unstructured":"Zheng Y, Yu Q, Lin Y, Zhou Y, Lan L, Yang S, Wu J (2022) Global burden and trends of sexually transmitted infections from 1990 to 2019: an observational trend study. 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