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Comparing direct plating to enrichment-based methods significantly increased detection sensitivity, especially for CPOs (from 17 to 27 cases, a 58.8% increase). Detection rates for ESBL producers and VRE also improved significantly (by 20%). Pre-enrichment is therefore a valuable addition to routine MDR bacteria screening protocols, which is particularly useful when high accuracy is needed for prevention and infection control as well as for outbreak management.<\/jats:p>","DOI":"10.1007\/s10096-025-05288-1","type":"journal-article","created":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T12:41:00Z","timestamp":1758890460000},"page":"3135-3138","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Optimized detection of ESBL producers, carbapenemase producers, and VRE from rectal swabs using enrichment broth for superior screening sensitivity"],"prefix":"10.1007","volume":"44","author":[{"given":"Dominique S","family":"Blanc","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Massiha","family":"Kamyar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrice","family":"Nordmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Poirel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gilbert","family":"Greub","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,9,26]]},"reference":[{"issue":"5","key":"5288_CR1","doi-asserted-by":"publisher","first-page":"412","DOI":"10.1089\/mdr.2015.0220","volume":"22","author":"E Cerceo","year":"2016","unstructured":"Cerceo E, Deitelzweig SB, Sherman BM, Amin AN (2016) Multidrug-resistant Gram-negative bacterial infections in the hospital setting: overview, implications for clinical practice, and emerging treatment options. 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