{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,5]],"date-time":"2026-11-05T22:15:02Z","timestamp":1793916902524,"version":"4.8.0"},"reference-count":20,"publisher":"Springer Science and Business Media LLC","issue":"2","license":[{"start":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T00:00:00Z","timestamp":1761609600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T00:00:00Z","timestamp":1761609600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100004047","name":"Karolinska Institute","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004047","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Eur J Clin Microbiol Infect Dis"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>The identification of filamentous fungi in clinical microbiology laboratories remains a challenging task. Although matrix-assisted laser desorption\/ionization\u2013time of flight mass spectrometry (MALDI-TOF MS) has revolutionized microbial diagnostics by enabling rapid and accurate species-level identification, its application to molds is still evolving. This study aims to evaluate the performance of two Bruker MALDI-TOF MS systems, Sirius One and Microflex 3.1, for the identification of filamentous fungi using different extraction protocols and database configurations.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method<\/jats:title>\n                    <jats:p>A total of 68 filamentous fungal isolates, including clinically significant species, were analyzed. Fungal cultures were processed under standardized conditions using two protein extraction methods: a detailed in-tube extraction with ethanol, formic acid, and acetonitrile, and a direct on-plate extraction. Spectra were acquired using both Sirius One and Microflex 3.1 systems, and identifications were performed using manufacturer-provided databases and the MSI-2.0 database.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The Sirius One system outperformed Microflex 3.1, achieving a 92.6% correct identification rate with the MSI-2 database compared to 70.6% for Microflex (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.01). When using manufacturer-provided databases, identification rates were lower: 51.5% for Sirius One and 41.2% for Microflex. Notably, the on-plate extraction method performed comparably to the in-tube method, achieving 94.1% accuracy with Sirius One and the MSI-2 database.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The combination of the Sirius One system, MSI-2.0 database, and on-plate extraction method provides a highly effective and time-efficient workflow for the identification of filamentous fungi in routine clinical diagnostics, reaching 94.1% accuracy. This approach is recommended for implementation in clinical mycology laboratories, though further optimization of manufacturer-supplied databases remains necessary.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s10096-025-05316-0","type":"journal-article","created":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T23:24:23Z","timestamp":1761607463000},"page":"395-402","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Optimizing filamentous fungi identification by MALDI-TOF MS: A comparative analysis of key factors"],"prefix":"10.1007","volume":"45","author":[{"given":"\u00d6zlem","family":"Dogan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Busra Betul","family":"Ozmen-Capin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"White","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiara","family":"Serra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sultan","family":"Ahmed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Botero-Kleiven","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Volkan","family":"\u00d6zenci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,10,28]]},"reference":[{"key":"5316_CR1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-07556-5","volume":"9","author":"BL Wickes","year":"2018","unstructured":"Wickes BL, Wiederhold NP (2018) Molecular diagnostics in medical mycology. 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No ethical approval was required as this is a retrospective laboratory study performed by archived isolates.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics statement"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}