{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T08:06:12Z","timestamp":1789200372239,"version":"build-2803163510"},"reference-count":26,"publisher":"Springer Science and Business Media LLC","license":[{"start":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T00:00:00Z","timestamp":1789171200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T00:00:00Z","timestamp":1789171200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"name":"Baoan District Science and Technology Innovation Bureau Fund","award":["2023JD234"],"award-info":[{"award-number":["2023JD234"]}]},{"DOI":"10.13039\/501100021171","name":"Basic and Applied Basic Research Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2023A1515220253"],"award-info":[{"award-number":["2023A1515220253"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Eur J Clin Microbiol Infect Dis"],"DOI":"10.1007\/s10096-026-05666-3","type":"journal-article","created":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T07:21:08Z","timestamp":1789197668000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Rapid Molecular Detection Method for Streptococcus agalactiae Based on Bacteriophage Lysin Lysis and Real-Time PCR"],"prefix":"10.1007","author":[{"given":"Huijun","family":"Zhu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoying","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuanmei","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yixuan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangxian","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helu","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhong","family":"Dou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,9,12]]},"reference":[{"key":"5666_CR1","doi-asserted-by":"publisher","first-page":"e0015422","DOI":"10.1128\/cmr.00154-22","volume":"38","author":"G Manuel","year":"2025","unstructured":"Manuel G, Twentyman J, Noble K et al (2025) Group B streptococcal infections in pregnancy and early life. Clin Microbiol Rev 38:e0015422. https:\/\/doi.org\/10.1128\/cmr.00154-22","journal-title":"Clin Microbiol Rev"},{"key":"5666_CR2","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.GPP3-0007-2018","author":"VN Raabe","year":"2019","unstructured":"Raabe VN, Shane AL (2019) Group B Streptococcus (Streptococcus agalactiae). Microbiol Spectr. https:\/\/doi.org\/10.1128\/microbiolspec.GPP3-0007-2018. 7","journal-title":"Microbiol Spectr"},{"key":"5666_CR3","doi-asserted-by":"publisher","first-page":"e807","DOI":"10.1016\/s2214-109x(22)00093-6","volume":"10","author":"BP Gon\u00e7alves","year":"2022","unstructured":"Gon\u00e7alves BP, Procter SR, Paul P et al (2022) Group B streptococcus infection during pregnancy and infancy: estimates of regional and global burden. Lancet Glob Health 10:e807\u2013e819. https:\/\/doi.org\/10.1016\/s2214-109x(22)00093-6","journal-title":"Lancet Glob Health"},{"key":"5666_CR4","doi-asserted-by":"publisher","first-page":"896","DOI":"10.1056\/NEJMra2313146","volume":"394","author":"KM Puopolo","year":"2026","unstructured":"Puopolo KM (2026) Group B Streptococcal Disease. N Engl J Med 394:896\u2013905. https:\/\/doi.org\/10.1056\/NEJMra2313146","journal-title":"N Engl J Med"},{"key":"5666_CR5","doi-asserted-by":"publisher","unstructured":"(2019) Prevention of group B streptococcal early-onset disease in newborns. Pediatrics https:\/\/doi.org\/10.1542\/peds.2019-1882","DOI":"10.1542\/peds.2019-1882"},{"key":"5666_CR6","doi-asserted-by":"publisher","first-page":"285","DOI":"10.1186\/1471-2334-10-285","volume":"10","author":"NA El Aila","year":"2010","unstructured":"El Aila NA, Tency I, Claeys G et al (2010) Comparison of different sampling techniques and of different culture methods for detection of group B streptococcus carriage in pregnant women. BMC Infect Dis 10:285. https:\/\/doi.org\/10.1186\/1471-2334-10-285","journal-title":"BMC Infect Dis"},{"key":"5666_CR7","doi-asserted-by":"publisher","first-page":"928","DOI":"10.1128\/JCM.43.2.928-930.2005","volume":"43","author":"M Rosa-Fraile","year":"2005","unstructured":"Rosa-Fraile M, Camacho-Munoz E, Rodriguez-Granger J, Liebana-Martos C (2005) Specimen storage in transport medium and detection of group B streptococci by culture. J Clin Microbiol 43:928\u2013930. https:\/\/doi.org\/10.1128\/JCM.43.2.928-930.2005","journal-title":"J Clin Microbiol"},{"key":"5666_CR8","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3310\/hta13420","volume":"13","author":"J Daniels","year":"2009","unstructured":"Daniels J, Gray J, Pattison H et al (2009) Rapid testing for group B streptococcus during labour: a test accuracy study with evaluation of acceptability and cost-effectiveness. Health Technol Assess 13:1\u2013154iii. https:\/\/doi.org\/10.3310\/hta13420","journal-title":"Health Technol Assess"},{"key":"5666_CR9","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1186\/s12941-024-00726-y","volume":"23","author":"L Lu","year":"2024","unstructured":"Lu L, Chen Y, Wang Q, Gao J, Ying C (2024) To evaluate the performance of simultaneous amplification and testing assay for group B Streptococcus detection: comparison with real-time PCR and ddPCR assays. Ann Clin Microbiol Antimicrob 23:65. https:\/\/doi.org\/10.1186\/s12941-024-00726-y","journal-title":"Ann Clin Microbiol Antimicrob"},{"key":"5666_CR10","doi-asserted-by":"publisher","first-page":"276","DOI":"10.1097\/AOG.0000000000003057","volume":"133","author":"N El Helali","year":"2019","unstructured":"El Helali N, Habibi F, Azria E et al (2019) Point-of-Care Intrapartum Group B Streptococcus Molecular Screening: Effectiveness and Costs. Obstet Gynecol 133:276\u2013281. https:\/\/doi.org\/10.1097\/AOG.0000000000003057","journal-title":"Obstet Gynecol"},{"key":"5666_CR11","doi-asserted-by":"publisher","first-page":"3212","DOI":"10.1128\/jb.181.10.3212-3219.1999","volume":"181","author":"B Spellerberg","year":"1999","unstructured":"Spellerberg B, Pohl B, Haase G, Martin S, Weber-Heynemann J, L\u00fctticken R (1999) Identification of genetic determinants for the hemolytic activity of Streptococcus agalactiae by ISS1 transposition. J Bacteriol 181:3212\u20133219. https:\/\/doi.org\/10.1128\/jb.181.10.3212-3219.1999","journal-title":"J Bacteriol"},{"key":"5666_CR12","doi-asserted-by":"publisher","first-page":"5984","DOI":"10.1128\/jb.00768-05","volume":"188","author":"B Gottschalk","year":"2006","unstructured":"Gottschalk B, Br\u00f6ker G, Kuhn M, Aymanns S, Gleich-Theurer U, Spellerberg B (2006) Transport of multidrug resistance substrates by the Streptococcus agalactiae hemolysin transporter. J Bacteriol 188:5984\u20135992. https:\/\/doi.org\/10.1128\/jb.00768-05","journal-title":"J Bacteriol"},{"key":"5666_CR13","doi-asserted-by":"publisher","first-page":"1150","DOI":"10.1128\/aem.61.3.1150-1152.1995","volume":"61","author":"MJ Loessner","year":"1995","unstructured":"Loessner MJ, Schneider A, Scherer S (1995) A new procedure for efficient recovery of DNA, RNA, and proteins from Listeria cells by rapid lysis with a recombinant bacteriophage endolysin. Appl Environ Microbiol 61:1150\u20131152. https:\/\/doi.org\/10.1128\/aem.61.3.1150-1152.1995","journal-title":"Appl Environ Microbiol"},{"key":"5666_CR14","doi-asserted-by":"publisher","first-page":"357","DOI":"10.1016\/j.ijmm.2010.04.002","volume":"300","author":"VA Fischetti","year":"2010","unstructured":"Fischetti VA (2010) Bacteriophage endolysins: a novel anti-infective to control Gram-positive pathogens. Int J Med Microbiol 300:357\u2013362. https:\/\/doi.org\/10.1016\/j.ijmm.2010.04.002","journal-title":"Int J Med Microbiol"},{"key":"5666_CR15","doi-asserted-by":"publisher","first-page":"4808","DOI":"10.1128\/jb.186.14.4808-4812.2004","volume":"186","author":"P Yoong","year":"2004","unstructured":"Yoong P, Schuch R, Nelson D, Fischetti VA (2004) Identification of a broadly active phage lytic enzyme with lethal activity against antibiotic-resistant Enterococcus faecalis and Enterococcus faecium. J Bacteriol 186:4808\u20134812. https:\/\/doi.org\/10.1128\/jb.186.14.4808-4812.2004","journal-title":"J Bacteriol"},{"key":"5666_CR16","doi-asserted-by":"publisher","first-page":"210","DOI":"10.1111\/1751-7915.12166","volume":"8","author":"Q Dong","year":"2015","unstructured":"Dong Q, Wang J, Yang H et al (2015) Construction of a chimeric lysin Ply187N-V12C with extended lytic activity against staphylococci and streptococci. Microb Biotechnol 8:210\u2013220. https:\/\/doi.org\/10.1111\/1751-7915.12166","journal-title":"Microb Biotechnol"},{"key":"5666_CR17","doi-asserted-by":"publisher","first-page":"975449","DOI":"10.3389\/fcimb.2022.975449","volume":"12","author":"H Gou","year":"2022","unstructured":"Gou H, Zhang J, Liao M (2022) Editorial: Point-of-care testing for infectious and foodborne pathogens. Front Cell Infect Microbiol 12:975449. https:\/\/doi.org\/10.3389\/fcimb.2022.975449","journal-title":"Front Cell Infect Microbiol"},{"key":"5666_CR18","doi-asserted-by":"publisher","DOI":"10.1002\/ijgo.70977","author":"E P\u00e1ez-Castellanos","year":"2026","unstructured":"P\u00e1ez-Castellanos E, Burgos-C\u00e1rdenas AJ, Gait\u00e1n-Duarte H, Rubio-Romero JA, Vargas-Rodriguez DD, Sanabria-Mora F (2026) Rapid tests for detecting group B streptococcus colonization in pregnant women: A systematic review and meta-analysis of diagnostic accuracy. Int J Gynaecol Obstet. https:\/\/doi.org\/10.1002\/ijgo.70977","journal-title":"Int J Gynaecol Obstet"},{"key":"5666_CR19","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms122483","author":"Y Liu","year":"2022","unstructured":"Liu Y, Liu J (2022) Group B Streptococcus: Virulence Factors and Pathogenic Mechanism. Microorganisms. https:\/\/doi.org\/10.3390\/microorganisms10122483","journal-title":"Microorganisms"},{"key":"5666_CR20","doi-asserted-by":"publisher","first-page":"1066","DOI":"10.1016\/j.cmi.2018.01.008","volume":"24","author":"A Curry","year":"2018","unstructured":"Curry A, Bookless G, Donaldson K, Knowles SJ (2018) Evaluation of hibergene loop-mediated isothermal amplification assay for detection of group B streptococcus in recto-vaginal swabs: a prospective diagnostic accuracy study. Clin Microbiol Infect 24:1066\u20131069. https:\/\/doi.org\/10.1016\/j.cmi.2018.01.008","journal-title":"Clin Microbiol Infect"},{"key":"5666_CR21","doi-asserted-by":"publisher","first-page":"20251197","DOI":"10.1515\/med-2025-1197","volume":"20","author":"VA Arsenijevic","year":"2025","unstructured":"Arsenijevic VA, Gerginic V, Milicic B, Jurisic A, Petricevic L (2025) Screening of Group B Streptococcus in pregnancy: A systematic review for the laboratory detection. Open Med (Wars) 20:20251197. https:\/\/doi.org\/10.1515\/med-2025-1197","journal-title":"Open Med (Wars)"},{"key":"5666_CR22","doi-asserted-by":"publisher","first-page":"660","DOI":"10.1373\/clinchem.2013.213504","volume":"60","author":"RK Daher","year":"2014","unstructured":"Daher RK, Stewart G, Boissinot M, Bergeron MG (2014) Isothermal recombinase polymerase amplification assay applied to the detection of group B streptococci in vaginal\/anal samples. Clin Chem 60:660\u2013666. https:\/\/doi.org\/10.1373\/clinchem.2013.213504","journal-title":"Clin Chem"},{"key":"5666_CR23","doi-asserted-by":"publisher","unstructured":"Guo XG, Zhuang YR, Wen JZ et al (2019) Evaluation of the real-time fluorescence loop-mediated isothermal amplification assay for the detection of Streptococcus agalactiae. Biosci Rep 39. https:\/\/doi.org\/10.1042\/BSR20190383","DOI":"10.1042\/BSR20190383"},{"key":"5666_CR24","doi-asserted-by":"publisher","unstructured":"Gomes AFN, de Castro FFA, Silva MR et al (2024) Interference of streptococcus agalactiae blitz therapy in staphylococcus aureus microbiological diagnosis in subclinical bovine mastitis. Vet Sci 11. https:\/\/doi.org\/10.3390\/vetsci11060233","DOI":"10.3390\/vetsci11060233"},{"key":"5666_CR25","doi-asserted-by":"publisher","first-page":"2141","DOI":"10.1002\/jor.24935","volume":"39","author":"A Sulovari","year":"2021","unstructured":"Sulovari A, Ninomiya MJ, Beck CA et al (2021) Clinical utilization of species-specific immunoassays for identification of Staphylococcus aureus and Streptococcus agalactiae in orthopedic infections. J Orthop Res 39:2141\u20132150. https:\/\/doi.org\/10.1002\/jor.24935","journal-title":"J Orthop Res"},{"key":"5666_CR26","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms020216","author":"Y Shan","year":"2020","unstructured":"Shan Y, Yang N, Teng D et al (2020) Recombinant of the Staphylococcal Bacteriophage Lysin CHAP(k) and Its Elimination against Streptococcus agalactiae Biofilms. Microorganisms. https:\/\/doi.org\/10.3390\/microorganisms8020216","journal-title":"Microorganisms"}],"container-title":["European Journal of Clinical Microbiology &amp; Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10096-026-05666-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10096-026-05666-3","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10096-026-05666-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T07:21:09Z","timestamp":1789197669000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10096-026-05666-3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,12]]},"references-count":26,"alternative-id":["5666"],"URL":"https:\/\/doi.org\/10.1007\/s10096-026-05666-3","relation":{},"ISSN":["0934-9723","1435-4373"],"issn-type":[{"value":"0934-9723","type":"print"},{"value":"1435-4373","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,12]]},"assertion":[{"value":"4 July 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"3 September 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"12 September 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The study was approved by the Institutional Review Board of Shenzhen Hospital of Integrated Traditional Chinese and Western Medicine (KY-2023-054-02). All participants were informed about the study protocol and provided written informed consent to participate in the study. I confirm that all methods were performed in accordance with the relevant guidelines. All procedures were performed in accordance with the ethical standards laid down in the 1964 Declaration of Helsinki and its later amendments.","order":1,"name":"Ethics","label":"Ethics approval and consent to participate","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Not applicable.","order":2,"name":"Ethics","label":"Consent for publication","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","label":"Competing interests","group":{"name":"EthicsHeading","label":"Declarations"}}]}}