{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T01:28:21Z","timestamp":1778722101922,"version":"3.51.4"},"reference-count":44,"publisher":"Springer Science and Business Media LLC","issue":"3","license":[{"start":{"date-parts":[[2014,12,4]],"date-time":"2014-12-04T00:00:00Z","timestamp":1417651200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Lasers Med Sci"],"published-print":{"date-parts":[[2015,4]]},"DOI":"10.1007\/s10103-014-1689-y","type":"journal-article","created":{"date-parts":[[2014,12,3]],"date-time":"2014-12-03T03:07:06Z","timestamp":1417576026000},"page":"1031-1039","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["A new model of in vitro fungal biofilms formed on human nail fragments allows reliable testing of laser and light therapies against onychomycosis"],"prefix":"10.1007","volume":"30","author":[{"given":"Taissa Vieira Machado","family":"Vila","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sonia","family":"Rozental","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia Maria Duarte","family":"de S\u00e1 Guimar\u00e3es","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2014,12,4]]},"reference":[{"key":"1689_CR1","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1046\/j.1440-0960.2002.t01-1-00570.x","volume":"43","author":"S Murray","year":"2002","unstructured":"Murray S, Dawber R (2002) Onychomycosis of toenails: orthopaedic and podiatric considerations. Australas J Dermatol 43:105\u2013112","journal-title":"Australas J Dermatol"},{"key":"1689_CR2","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1067\/mjd.2000.107754","volume":"43","author":"MA Ghannoum","year":"2000","unstructured":"Ghannoum MA, Hajjeh RA, Scher R et al (2000) A large-scale North American study of fungal isolates from nails: the frequency of onychomycosis, fungal distribution, and antifungal susceptibility patterns. J Am Acad Dermatol 43:641\u2013648. doi: 10.1067\/mjd.2000.107754","journal-title":"J Am Acad Dermatol"},{"key":"1689_CR3","first-page":"188","volume":"12","author":"P Nenoff","year":"2014","unstructured":"Nenoff P, Kr\u00fcger C, Ginter-Hanselmayer G, Tietz H-J (2014) Mycology\u2014an update. Part 1: dermatomycoses: causative agents, epidemiology and pathogenesis. J Dtsch Dermatol Ges 12:188\u2013212. doi: 10.1111\/ddg.12245","journal-title":"J Dtsch Dermatol Ges"},{"key":"1689_CR4","doi-asserted-by":"crossref","first-page":"748","DOI":"10.1016\/S0190-9622(03)02117-0","volume":"50","author":"K Foster","year":"2004","unstructured":"Foster K, Ghannoum M, Elewski B (2004) Epidemiologic surveillance of cutaneous fungal infection in the United States from 1999 to 2002. J Am Acad Dermatol 50:748\u2013752","journal-title":"J Am Acad Dermatol"},{"key":"1689_CR5","doi-asserted-by":"crossref","first-page":"108","DOI":"10.4103\/0255-0857.40522","volume":"26","author":"R Kaur","year":"2008","unstructured":"Kaur R, Kashyap B, Bhalla P (2008) Onychomycosis\u2014epidemiology, diagnosis and management. Indian J Med Microbiol 26:108\u2013116","journal-title":"Indian J Med Microbiol"},{"key":"1689_CR6","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1590\/S0365-05962003000300006","volume":"78","author":"AJ Ara\u00fajo De","year":"2003","unstructured":"De Ara\u00fajo AJ, Souza MAJ, Bastos OM, de Oliveira JC (2003) Ocorr\u00eancia de onicomicose em pacientes atendidos em consult\u00f3rios dermatol\u00f3gicos da cidade do Rio de Janeiro. An Bras Dermatol 78:299\u2013308","journal-title":"An Bras Dermatol"},{"key":"1689_CR7","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1007\/s11046-009-9212-x","volume":"168","author":"J Jayatilake","year":"2009","unstructured":"Jayatilake J, Tilakaratne W, Panagoda G (2009) Candidal onychomycosis: a mini-review. Mycopathologia 168:165\u2013173. doi: 10.1007\/s11046-009-9212-x","journal-title":"Mycopathologia"},{"key":"1689_CR8","doi-asserted-by":"crossref","first-page":"1610","DOI":"10.1128\/AAC.02056-12","volume":"57","author":"W Jo Siu","year":"2013","unstructured":"Jo Siu W, Tatsumi Y, Senda H et al (2013) Comparison of in vitro antifungal activities of efinaconazole and currently available antifungal agents against a variety of pathogenic fungi associated with onychomycosis. Antimicrob Agents Chemother 57:1610\u20131616","journal-title":"Antimicrob Agents Chemother"},{"key":"1689_CR9","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1128\/AAC.50.3.917-921.2006","volume":"50","author":"M Cuenca-Estrella","year":"2006","unstructured":"Cuenca-Estrella M, Gomez-Lopez A, Mellado E et al (2006) Head-to-head comparison of the activities of currently available antifungal agents against 3,378 Spanish clinical isolates of yeasts and filamentous fungi. Antimicrob Agents Chemother 50:917\u2013921. doi: 10.1128\/AAC.50.3.917","journal-title":"Antimicrob Agents Chemother"},{"key":"1689_CR10","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1093\/jac\/dkn022","volume":"61","author":"A Alastruey-Izquierdo","year":"2008","unstructured":"Alastruey-Izquierdo A, Cuenca-Estrella M, Monz\u00f3n A et al (2008) Antifungal susceptibility profile of clinical Fusarium spp. isolates identified by molecular methods. J Antimicrob Chemother 61:805\u2013809. doi: 10.1093\/jac\/dkn022","journal-title":"J Antimicrob Chemother"},{"key":"1689_CR11","first-page":"191","volume":"85","author":"GP Bourgeois","year":"2010","unstructured":"Bourgeois GP, Cafardi JA, Sellheyer K, Andea AA (2010) Disseminated Fusarium originating from toenail paronychia in a neutropenic patient. Cutis 85:191\u2013194","journal-title":"Cutis"},{"key":"1689_CR12","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1067\/mjd.2002.124699","volume":"47","author":"CN Burkharta","year":"2002","unstructured":"Burkharta CN, Burkhart CG, Gupta AK (2002) Dermatophytoma: recalcitrance to treatment because of existence of fungal biofilm. J Am Acad Dermatol 47:629\u2013631. doi: 10.1067\/mjd.2002.124699","journal-title":"J Am Acad Dermatol"},{"key":"1689_CR13","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1016\/j.jaad.2006.12.019","volume":"56","author":"RK Scher","year":"2007","unstructured":"Scher RK, Tavakkol A, Sigurgeirsson B et al (2007) Onychomycosis: diagnosis and definition of cure. J Am Acad Dermatol 56:939\u2013944. doi: 10.1016\/j.jaad.2006.12.019","journal-title":"J Am Acad Dermatol"},{"key":"1689_CR14","first-page":"496","volume":"11","author":"U Kimura","year":"2012","unstructured":"Kimura U, Takeuchi K, Kinoshita A et al (2012) Treating onychomycosis of the toenail: clinical efficacy of the sub-millisecond 1,064\u00a0nm Nd:YAG laser using a 5\u00a0mm spot diameter. J Drugs Dermatol 11:496\u2013504","journal-title":"J Drugs Dermatol"},{"key":"1689_CR15","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1002\/lsm.22211","volume":"46","author":"AE Ortiz","year":"2013","unstructured":"Ortiz AE, Avram MM, Wanner MA (2013) A review of lasers and light for the treatment of onychomycosis. Lasers Surg Med 46:117\u2013124. doi: 10.1002\/lsm.22211","journal-title":"Lasers Surg Med"},{"key":"1689_CR16","first-page":"4","volume":"17","author":"AK Gupta","year":"2012","unstructured":"Gupta AK, Simpson F (2012) Device-based therapies for onychomycosis treatment. Skin Ther Lett 17:4\u20139","journal-title":"Skin Ther Lett"},{"key":"1689_CR17","doi-asserted-by":"crossref","first-page":"1364","DOI":"10.1111\/j.1751-1097.2009.00615.x","volume":"85","author":"E Bornstein","year":"2009","unstructured":"Bornstein E, Hermans W, Gridley S, Manni J (2009) Near-infrared photoinactivation of bacteria and fungi at physiologic temperatures. Photochem Photobiol 85:1364\u20131374","journal-title":"Photochem Photobiol"},{"key":"1689_CR18","doi-asserted-by":"crossref","first-page":"166","DOI":"10.7547\/1000166","volume":"100","author":"A Landsman","year":"2010","unstructured":"Landsman A, Robbins A, Angelini P et al (2010) Treatment of mild, moderate, and severe onychomycosis using 870- and 930-nm light exposure. J Am Podiatr Med Assoc 100:166\u2013177","journal-title":"J Am Podiatr Med Assoc"},{"key":"1689_CR19","doi-asserted-by":"crossref","first-page":"2","DOI":"10.3109\/14764172.2011.552616","volume":"13","author":"L Hochman","year":"2011","unstructured":"Hochman L (2011) Laser treatment of onychomycosis using a novel .65 millisecond pulsed ND:YAG 1064\u00a0nm laser. J Cosmet Laser Ther 13:2\u20135","journal-title":"J Cosmet Laser Ther"},{"key":"1689_CR20","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1007\/s10103-007-0492-4","volume":"23","author":"E Vural","year":"2007","unstructured":"Vural E, Winfield HL, Shingleton AW et al (2007) The effects of laser irradiation on Trichophyton rubrum growth. Lasers Med Sci 23:349\u2013353. doi: 10.1007\/s10103-007-0492-4","journal-title":"Lasers Med Sci"},{"key":"1689_CR21","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1002\/lsm.10128","volume":"32","author":"G Meral","year":"2003","unstructured":"Meral G, Tasar F, Kocag\u00f6z S, Sener C (2003) Factors affecting the antibacterial effects of Nd:YAG laser in vivo. Lasers Surg Med 32:197\u2013202. doi: 10.1002\/lsm.10128","journal-title":"Lasers Surg Med"},{"key":"1689_CR22","doi-asserted-by":"crossref","first-page":"105","DOI":"10.5978\/islsm.12-OR-06","volume":"21","author":"G Lee","year":"2012","unstructured":"Lee G (2012) Inflammatory acne in the Asian skin type III treated with a square pulse, time resolved spectral distribution IPL system: a preliminary study. Laser Ther 21:105\u2013111","journal-title":"Laser Ther"},{"key":"1689_CR23","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/S0923-1811(02)00068-3","volume":"30","author":"A Kawada","year":"2002","unstructured":"Kawada A, Aragane Y, Kameyama H, Sangen Y (2002) Acne phototherapy with a high-intensity, enhanced, narrow-band, blue light source: an open study and in vitro investigation. J Dermatol Sci 30:129\u2013135","journal-title":"J Dermatol Sci"},{"key":"1689_CR24","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1080\/14764170410023785","volume":"6","author":"T Omi","year":"2004","unstructured":"Omi T, Bjerring P, Sato S et al (2004) 420\u00a0nm intense continuous light therapy for acne. J Cosmet Laser Ther 6:156\u2013162. doi: 10.1080\/14764170410023785","journal-title":"J Cosmet Laser Ther"},{"key":"1689_CR25","doi-asserted-by":"crossref","first-page":"308","DOI":"10.3109\/14764172.2011.630081","volume":"13","author":"MH Gold","year":"2011","unstructured":"Gold MH, Sensing W, Biron JA (2011) Clinical efficacy of home-use blue-light therapy for mild-to moderate acne. J Cosmet Laser Ther 13:308\u2013314. doi: 10.3109\/14764172.2011.630081","journal-title":"J Cosmet Laser Ther"},{"key":"1689_CR26","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1111\/j.1529-8019.2012.01519.x","volume":"25","author":"AK Gupta","year":"2012","unstructured":"Gupta AK, Simpson F (2012) Medical devices for the treatment of onychomycosis. Dermatol Ther (Heidelb) 25:574\u2013581","journal-title":"Dermatol Ther (Heidelb)"},{"key":"1689_CR27","first-page":"33","volume":"12","author":"P Nenoff","year":"2013","unstructured":"Nenoff P, Grunewald S, Paasch U (2013) Laser therapy of onychomycosis. J Dtsch Dermatol Ges 12:33\u201338. doi: 10.1111\/ddg.12251","journal-title":"J Dtsch Dermatol Ges"},{"key":"1689_CR28","doi-asserted-by":"crossref","first-page":"428","DOI":"10.7547\/1020428","volume":"102","author":"AK Gupta","year":"2012","unstructured":"Gupta AK, Simpson F (2012) Newly approved laser systems for onychomycosis. J Am Podiatr Med Assoc 102:428\u2013430","journal-title":"J Am Podiatr Med Assoc"},{"key":"1689_CR29","first-page":"3697","volume":"125","author":"Z Xu","year":"2012","unstructured":"Xu Z, Xu J, Zhuo F et al (2012) Effects of laser irradiation on Trichophyton rubrum growth and ultrastructure. Chin Med J (Engl) 125:3697\u20133700","journal-title":"Chin Med J (Engl)"},{"key":"1689_CR30","first-page":"913","volume":"10","author":"H Hees","year":"2012","unstructured":"Hees H, Raulin C, Baumler W (2012) Laser treatment of onychomycosis: an in vitro pilot study. J Dtsch Dermatol Ges 10:913\u2013918","journal-title":"J Dtsch Dermatol Ges"},{"key":"1689_CR31","doi-asserted-by":"crossref","first-page":"544","DOI":"10.3109\/02656736.2013.823672","volume":"29","author":"U Paasch","year":"2013","unstructured":"Paasch U, Mock A, Grunewald S et al (2013) Antifungal efficacy of lasers against dermatophytes and yeasts in vitro. Int J Hyperth 29:544\u2013550","journal-title":"Int J Hyperth"},{"key":"1689_CR32","first-page":"1","volume":"17","author":"AG Nusbaum","year":"2012","unstructured":"Nusbaum AG, Kirsner RS, Charles CA (2012) Biofilms in dermatology. Skin Ther Lett 17:1\u20135","journal-title":"Skin Ther Lett"},{"key":"1689_CR33","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1093\/jac\/dks353","volume":"68","author":"TVM Vila","year":"2012","unstructured":"Vila TVM, Ishida K, de Souza W et al (2012) Effect of alkylphospholipids on Candida albicans biofilm formation and maturation. J Antimicrob Chemother 68:113\u2013125. doi: 10.1093\/jac\/dks353","journal-title":"J Antimicrob Chemother"},{"key":"1689_CR34","doi-asserted-by":"crossref","first-page":"5385","DOI":"10.1128\/JB.183.18.5385-5394.2001","volume":"183","author":"J Chandra","year":"2001","unstructured":"Chandra J, Kuhn DM, Mukherjee PK et al (2001) Biofilm formation by the fungal pathogen Candida albicans: development, architecture, and drug resistance. J Bacteriol 183:5385\u20135394. doi: 10.1128\/JB.183.18.5385-5394.2001","journal-title":"J Bacteriol"},{"key":"1689_CR35","first-page":"163","volume":"18","author":"G Ramage","year":"2001","unstructured":"Ramage G, VandeWalle K, Wickes BL, Lopez-Ribot JL (2001) Characteristics of biofilm formation by Candida albicans. Rev Iberoam Micol 18:163\u2013170","journal-title":"Rev Iberoam Micol"},{"key":"1689_CR36","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1046\/j.1365-2133.149.s65.4.x","volume":"149","author":"J Faergemann","year":"2003","unstructured":"Faergemann J, Baran R (2003) Epidemiology, clinical presentation and diagnosis of onychomycosis. Br J Dermatol 149:1\u20134","journal-title":"Br J Dermatol"},{"key":"1689_CR37","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1111\/j.1365-4362.1995.tb02942.x","volume":"34","author":"DL Greer","year":"1995","unstructured":"Greer DL (1995) Evolving role of nondermatophytes in onychomycosis. Int J Dermatol 34:521\u2013524","journal-title":"Int J Dermatol"},{"key":"1689_CR38","doi-asserted-by":"crossref","first-page":"8795","DOI":"10.1128\/AEM.71.12.8795-8801.2005","volume":"71","author":"J Chandra","year":"2005","unstructured":"Chandra J, Patel JD, Li J et al (2005) Modification of surface properties of biomaterials influences the ability of Candida albicans to form biofilms. Appl Environ Microbiol 71:8795\u20138801. doi: 10.1128\/AEM.71.12.8795\u20138801.2005","journal-title":"Appl Environ Microbiol"},{"key":"1689_CR39","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/S1130-1406(08)70009-3","volume":"25","author":"G Ramage","year":"2008","unstructured":"Ramage G, Wickes BL, Lopez-Ribot JL (2008) A seed and feed model for the formation of Candida albicans biofilms under flow conditions using an improved modified Robbins device. Rev Iberoam Micol 25:37\u201340","journal-title":"Rev Iberoam Micol"},{"key":"1689_CR40","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1128\/AAC.00387-07","volume":"52","author":"Y Imamura","year":"2008","unstructured":"Imamura Y, Chandra J, Mukherjee PK et al (2008) Fusarium and Candida albicans biofilms on soft contact lenses: model development, influence of lens type, and susceptibility to lens care solutions. Antimicrob Agents Chemother 52:171\u2013182. doi: 10.1128\/AAC. 00387-07","journal-title":"Antimicrob Agents Chemother"},{"key":"1689_CR41","doi-asserted-by":"crossref","first-page":"4450","DOI":"10.1167\/iovs.12-9848","volume":"53","author":"PK Mukherjee","year":"2012","unstructured":"Mukherjee PK, Chandra J, Yu C et al (2012) Characterization of Fusarium keratitis outbreak isolates: contribution of biofilms to antimicrobial resistance and pathogenesis. Invest Ophthalmol Vis Sci 53:4450\u20134457. doi: 10.1167\/iovs. 12-9848","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"1689_CR42","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1111\/j.1751-1097.2009.00672.x","volume":"86","author":"Z Manevitch","year":"2010","unstructured":"Manevitch Z, Lev D, Hochberg M et al (2010) Direct antifungal effect of femtosecond laser on Trichophyton rubrum onychomycosis. Photochem Photobiol 86:476\u2013479","journal-title":"Photochem Photobiol"},{"key":"1689_CR43","doi-asserted-by":"crossref","first-page":"1168","DOI":"10.1111\/j.1468-3083.2012.04487.x","volume":"27","author":"X Fan","year":"2013","unstructured":"Fan X, Xing Y-Z, Liu L-H et al (2013) Effects of 420-nm intense pulsed light in an acne animal model. J Eur Acad Dermatol Venereol 27:1168\u20131171. doi: 10.1111\/j.1468-3083.2012.04487.x","journal-title":"J Eur Acad Dermatol Venereol"},{"key":"1689_CR44","first-page":"1","volume":"1","author":"J Kozarev","year":"2010","unstructured":"Kozarev J, Vizintin Z (2010) Novel laser therapy in treatment of onychomycosis. J Laser Health Acad 1:1\u20138","journal-title":"J Laser Health Acad"}],"container-title":["Lasers in Medical Science"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s10103-014-1689-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s10103-014-1689-y\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s10103-014-1689-y","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,8,17]],"date-time":"2019-08-17T23:10:01Z","timestamp":1566083401000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s10103-014-1689-y"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,12,4]]},"references-count":44,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2015,4]]}},"alternative-id":["1689"],"URL":"https:\/\/doi.org\/10.1007\/s10103-014-1689-y","relation":{},"ISSN":["0268-8921","1435-604X"],"issn-type":[{"value":"0268-8921","type":"print"},{"value":"1435-604X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,12,4]]}}}