{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T12:29:26Z","timestamp":1789907366841,"version":"4.0.1"},"reference-count":39,"publisher":"Springer Science and Business Media LLC","issue":"6","license":[{"start":{"date-parts":[[2018,2,15]],"date-time":"2018-02-15T00:00:00Z","timestamp":1518652800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Lasers Med Sci"],"published-print":{"date-parts":[[2018,8]]},"DOI":"10.1007\/s10103-018-2453-5","type":"journal-article","created":{"date-parts":[[2018,2,15]],"date-time":"2018-02-15T08:21:12Z","timestamp":1518682872000},"page":"1189-1195","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Effects of 915\u00a0nm laser irradiation on human osteoblasts: a preliminary in vitro study"],"prefix":"10.1007","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0620-9222","authenticated-orcid":false,"given":"Giovanni","family":"Mergoni","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paolo","family":"Vescovi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvana","family":"Belletti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacopo","family":"Uggeri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samir","family":"Nammour","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rita","family":"Gatti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2018,2,15]]},"reference":[{"key":"2453_CR1","first-page":"1012","volume":"107","author":"E Mester","year":"1966","unstructured":"Mester E (1966) The use of the laser beam in therapy. Orv Hetil 107:1012\u20131016","journal-title":"Orv Hetil"},{"key":"2453_CR2","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/S1011-1344(98)00219-X","volume":"49","author":"T Karu","year":"1999","unstructured":"Karu T (1999) Primary and secondary mechanisms of action of visible to near-IR radiation on cells. J Photochem Photobiol B Biol 49:1\u201317","journal-title":"J Photochem Photobiol B Biol"},{"key":"2453_CR3","doi-asserted-by":"publisher","first-page":"3503","DOI":"10.1016\/j.biomaterials.2004.09.033","volume":"26","author":"M Khadra","year":"2005","unstructured":"Khadra M, Lyngstadaas SP, Haanaes HR, Mustafa K (2005) Effect of laser therapy on attachment, proliferation and differentiation of human osteoblast-like cells cultured on titanium implant material. Biomaterials 26:3503\u20133509","journal-title":"Biomaterials"},{"key":"2453_CR4","doi-asserted-by":"publisher","first-page":"247","DOI":"10.1111\/j.1834-7819.1997.tb00129.x","volume":"42","author":"LJ Walsh","year":"1997","unstructured":"Walsh LJ (1997) The current status of low level laser therapy in dentistry. Part 1. Soft tissue applications. Aust Dent J 42:247\u2013254","journal-title":"Aust Dent J"},{"key":"2453_CR5","doi-asserted-by":"publisher","first-page":"302","DOI":"10.1111\/j.1834-7819.1997.tb00134.x","volume":"42","author":"LJ Walsh","year":"1997","unstructured":"Walsh LJ (1997) The current status of low level laser therapy in dentistry. Part 2. Hard tissue applications. Aust Dent J 42:302\u2013306","journal-title":"Aust Dent J"},{"key":"2453_CR6","doi-asserted-by":"publisher","first-page":"602","DOI":"10.2203\/dose-response.11-009.Hamblin","volume":"9","author":"YY Huang","year":"2011","unstructured":"Huang YY, Sharma SK, Carroll J, Hamblin MR (2011) Biphasic dose response in low level light therapy\u2014an update. Dose Response 9:602\u2013618","journal-title":"Dose Response"},{"key":"2453_CR7","doi-asserted-by":"publisher","first-page":"803","DOI":"10.1111\/jop.12442","volume":"45","author":"PGB Heymann","year":"2016","unstructured":"Heymann PGB, Ziebart T, Kammerer PW et al (2016) The enhancing effect of a laser photochemotherapy with cisplatin or zolendronic acid in primary human osteoblasts and osteosarcoma cells in vitro. J Oral Pathol Med 45:803\u2013809","journal-title":"J Oral Pathol Med"},{"key":"2453_CR8","doi-asserted-by":"publisher","first-page":"785","DOI":"10.1111\/clr.12882","volume":"28","author":"IK Karoussis","year":"2017","unstructured":"Karoussis IK, Kyriakidou K, Psarros C, Lang NP, Vrotsos IA (2017) Nd:YAG laser radiation (1.064 nm) accelerates differentiation of osteoblasts to osteocytes on smooth and rough titanium surfaces in vitro. Clin Oral Implants Res 28:785\u2013790","journal-title":"Clin Oral Implants Res"},{"key":"2453_CR9","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1016\/j.jphotobiol.2016.08.006","volume":"163","author":"FA Oliveira","year":"2016","unstructured":"Oliveira FA, Matos AA, Santesso MR et al (2016) Low intensity lasers differently induce primary human osteoblast proliferation and differentiation. J Photochem Photobiol B Biol 163:14\u201321","journal-title":"J Photochem Photobiol B Biol"},{"key":"2453_CR10","doi-asserted-by":"publisher","first-page":"224","DOI":"10.15386\/cjmed-433","volume":"88","author":"L Crisan","year":"2015","unstructured":"Crisan L, Soritau O, Baciut M, Baciut G, Crisan BV (2015) The influence of laser radiation on human osteoblasts cultured on nanostructured composite substrates. Clujul Med 88:224\u2013232","journal-title":"Clujul Med"},{"key":"2453_CR11","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1089\/pho.2005.23.161","volume":"23","author":"A Stein","year":"2005","unstructured":"Stein A, Benayahu D, Maltz L, Oron U (2005) Low-level laser irradiation promotes proliferation and differentiation of human osteoblasts in vitro. Photomed Laser Surg 23:161\u2013166","journal-title":"Photomed Laser Surg"},{"key":"2453_CR12","doi-asserted-by":"publisher","first-page":"271","DOI":"10.1089\/104454703322564479","volume":"21","author":"Y Ueda","year":"2003","unstructured":"Ueda Y, Shimizu N (2003) Effects of pulse frequency of low-level laser therapy (LLLT) on bone nodule formation in rat calvarial cells. J Clin Laser Med Surg 21:271\u2013277","journal-title":"J Clin Laser Med Surg"},{"key":"2453_CR13","doi-asserted-by":"publisher","first-page":"078002","DOI":"10.1117\/1.JBO.20.7.078002","volume":"20","author":"M Tschon","year":"2015","unstructured":"Tschon M, Incerti Parenti S, Cepollaro S, Checchi L, Fini M (2015) Photobiomodulation with low-level diode laser promotes osteoblast migration in an in vitro micro wound model. J Biomed Opt 20:078002\u2013078010","journal-title":"J Biomed Opt"},{"key":"2453_CR14","first-page":"E12","volume":"60","author":"T Asai","year":"2014","unstructured":"Asai T, Suzuki H, Kitayama M et al (2014) The long-term effects of red light-emitting diode irradiation on the proliferation and differentiation of osteoblast-like MC3T3-E1 cells. Kobe J Med Sci 60:E12\u2013E18","journal-title":"Kobe J Med Sci"},{"key":"2453_CR15","doi-asserted-by":"publisher","first-page":"1463","DOI":"10.1007\/s10103-014-1556-x","volume":"29","author":"M Migliario","year":"2014","unstructured":"Migliario M, Pittarella P, Fanuli M, Rizzi M, Ren\u00f2 F (2014) Laser-induced osteoblast proliferation is mediated by ROS production. Lasers Med Sci 29:1463\u20131467","journal-title":"Lasers Med Sci"},{"key":"2453_CR16","doi-asserted-by":"publisher","first-page":"108002","DOI":"10.1117\/1.JBO.19.10.108002","volume":"19","author":"S Incerti Parenti","year":"2014","unstructured":"Incerti Parenti S, Checchi L, Fini M, Tschon M (2014) Different doses of low-level laser irradiation modulate the in vitro response of osteoblast-like cells. J Biomed Opt 19:108002\u2013108007","journal-title":"J Biomed Opt"},{"key":"2453_CR17","doi-asserted-by":"publisher","first-page":"47","DOI":"10.5978\/islsm.20.47","volume":"20","author":"M Kanenari","year":"2011","unstructured":"Kanenari M, Zhao J, Abiko Y (2011) Enhancement of microtubule-associated protein-1 alpha gene expression in osteoblasts by low level laser irradiation. Laser Ther 20:47\u201351","journal-title":"Laser Ther"},{"issue":"Suppl 1","key":"2453_CR18","doi-asserted-by":"publisher","first-page":"S167","DOI":"10.1089\/pho.2009.2693","volume":"28","author":"T Kiyosaki","year":"2010","unstructured":"Kiyosaki T, Mitsui N, Suzuki N, Shimizu N (2010) Low-level laser therapy stimulates mineralization via increased Runx2 expression and ERK phosphorylation in osteoblasts. Photomed Laser Surg 28(Suppl 1):S167\u2013S172","journal-title":"Photomed Laser Surg"},{"key":"2453_CR19","doi-asserted-by":"publisher","first-page":"1445","DOI":"10.1002\/jcb.22872","volume":"111","author":"S Hirata","year":"2010","unstructured":"Hirata S, Kitamura C, Kukushima H et al (2010) Low-level laser irradiation enhances BMP-induced osteoblast differentiation by stimulating the BMP\/Smad signaling pathway. J Cell Biochem 111:1445\u20131452","journal-title":"J Cell Biochem"},{"key":"2453_CR20","doi-asserted-by":"publisher","first-page":"551","DOI":"10.1002\/lsm.20521","volume":"39","author":"N Shimizu","year":"2007","unstructured":"Shimizu N, Mayahara K, Kiyosaki T et al (2007) Low-intensity laser irradiation stimulates bone nodule formation via insulin-like growth factor-I expression in rat calvarial cells. Lasers Surg Med 39:551\u2013559","journal-title":"Lasers Surg Med"},{"key":"2453_CR21","doi-asserted-by":"publisher","first-page":"78","DOI":"10.1007\/s10103-003-0255-9","volume":"18","author":"S Hamajima","year":"2003","unstructured":"Hamajima S, Hiratsuka K, Kiyama-Kishikawa M et al (2003) Effect of low-level laser irradiation on osteoglycin gene expression in osteoblasts. Lasers Med Sci 18:78\u201382","journal-title":"Lasers Med Sci"},{"key":"2453_CR22","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1007\/PL00011357","volume":"16","author":"M Yamamoto","year":"2001","unstructured":"Yamamoto M, Tamura K, Hiratsuka K, Abiko Y (2001) Stimulation of MCM3 gene expression in osteoblast by low level laser irradiation. Lasers Med Sci 16:213\u2013217","journal-title":"Lasers Med Sci"},{"key":"2453_CR23","doi-asserted-by":"publisher","first-page":"621","DOI":"10.1007\/s10103-011-1006-y","volume":"27","author":"TH Huang","year":"2012","unstructured":"Huang TH, Lu YC, Kao CT (2012) Low-level diode laser therapy reduces lipopolysaccharide (LPS)-induced bone cell inflammation. Lasers Med Sci 27:621\u2013627","journal-title":"Lasers Med Sci"},{"key":"2453_CR24","unstructured":"Bayat M, Virdi A, Rezaei F, Chien S (2017) Comparison of the in vitro effects of low-level laser therapy and low-intensity pulsed ultrasound therapy on bony cells and stem cells. Progress Biophys Molec Biol 1\u201313"},{"key":"2453_CR25","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1016\/0304-4165(84)90212-5","volume":"801","author":"JN Beresford","year":"1984","unstructured":"Beresford JN, Gallagher JA, Gowen M et al (1984) The effects of monocyte-conditioned medium and interleukin 1 on the synthesis of collagenous and non-collagenous proteins by mouse bone and human bone cells in vitro. Biochim Biophys Acta 801:58\u201365","journal-title":"Biochim Biophys Acta"},{"key":"2453_CR26","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1016\/j.ab.2004.02.002","volume":"329","author":"CA Gregory","year":"2004","unstructured":"Gregory CA, Gunn WG, Peister A, Prockop DJ (2004) An alizarin red-based assay of mineralization by adherent cells in culture: comparison with cetylpyridinium chloride extraction. Anal Biochem 329:77\u201384","journal-title":"Anal Biochem"},{"key":"2453_CR27","doi-asserted-by":"publisher","first-page":"1","DOI":"10.4161\/spmg.19885","volume":"2","author":"G Kaur","year":"2012","unstructured":"Kaur G, Dufour JM (2012) Cell lines: valuable tools or useless artifacts. Spermatogenesis 2:1\u20135","journal-title":"Spermatogenesis"},{"key":"2453_CR28","first-page":"255","volume":"49","author":"S Incerti Parenti","year":"2013","unstructured":"Incerti Parenti S, Panseri S, Gracco A et al (2013) Effect of low-level laser irradiation on osteoblast-like cells cultured on porous hydroxyapatite scaffolds. Ann Ist Super Sanita 49:255\u2013260","journal-title":"Ann Ist Super Sanita"},{"key":"2453_CR29","doi-asserted-by":"publisher","first-page":"112","DOI":"10.1007\/s00508-008-0932-6","volume":"120","author":"E Stein","year":"2008","unstructured":"Stein E, Koehn J, Sutter W et al (2008) Initial effects of low-level laser therapy on growth and differentiation of human osteoblast-like cells. Wien Klin Wochenschr 120:112\u2013117","journal-title":"Wien Klin Wochenschr"},{"key":"2453_CR30","doi-asserted-by":"publisher","first-page":"275","DOI":"10.1089\/pho.2007.2055","volume":"25","author":"ACM Renno","year":"2007","unstructured":"Renno ACM, McDonnell PA, Parizotto NA, Laakso E-L (2007) The effects of laser irradiation on osteoblast and osteosarcoma cell proliferation and differentiation in vitro. Photomed Laser Surg 25:275\u2013280","journal-title":"Photomed Laser Surg"},{"key":"2453_CR31","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1007\/s10103-012-1238-5","volume":"29","author":"MT Pagin","year":"2014","unstructured":"Pagin MT, de Oliveira FA, Oliveira RC et al (2014) Laser and light-emitting diode effects on pre-osteoblast growth and differentiation. Lasers Med Sci 29:55\u201359","journal-title":"Lasers Med Sci"},{"key":"2453_CR32","doi-asserted-by":"publisher","first-page":"332","DOI":"10.1002\/lsm.20298","volume":"38","author":"NA Fujihara","year":"2006","unstructured":"Fujihara NA, Hiraki KRN, Marques MM (2006) Irradiation at 780 nm increases proliferation rate of osteoblasts independently of dexamethasone presence. Lasers Surg Med 38:332\u2013336","journal-title":"Lasers Surg Med"},{"key":"2453_CR33","doi-asserted-by":"publisher","first-page":"347","DOI":"10.1016\/S8756-3282(97)00294-9","volume":"22","author":"Y Ozawa","year":"1998","unstructured":"Ozawa Y, Shimizu N, Kariya G, Abiko Y (1998) Low-energy laser irradiation stimulates bone nodule formation at early stages of cell culture in rat calvarial cells. Bone 22:347\u2013354","journal-title":"Bone"},{"key":"2453_CR34","doi-asserted-by":"publisher","first-page":"128006","DOI":"10.1117\/1.JBO.18.12.128006","volume":"18","author":"N Bloise","year":"2013","unstructured":"Bloise N, Ceccarelli G, Minzioni P et al (2013) Investigation of low-level laser therapy potentiality on proliferation and differentiation of human osteoblast-like cells in the absence\/presence of osteogenic factors. J Biomed Opt 18:128006","journal-title":"J Biomed Opt"},{"key":"2453_CR35","doi-asserted-by":"publisher","first-page":"7867","DOI":"10.1021\/acsnano.5b03567","volume":"9","author":"W Tang","year":"2015","unstructured":"Tang W, Tian J, Zheng Q et al (2015) Implantable self-powered low-level laser cure system for mouse embryonic osteoblasts\u2019 proliferation and differentiation. ACS Nano 9:7867\u20137873","journal-title":"ACS Nano"},{"key":"2453_CR36","doi-asserted-by":"publisher","first-page":"298","DOI":"10.1002\/lsm.20762","volume":"41","author":"S Saracino","year":"2009","unstructured":"Saracino S, Mozzati M, Martinasso G et al (2009) Superpulsed laser irradiation increases osteoblast activity via modulation of bone morphogenetic factors. Lasers Surg Med 41:298\u2013304","journal-title":"Lasers Surg Med"},{"key":"2453_CR37","doi-asserted-by":"publisher","first-page":"510","DOI":"10.1002\/lsm.20870","volume":"42","author":"IS Kim","year":"2010","unstructured":"Kim IS, Cho TH, Kim K, Weber FE, Hwang SJ (2010) High power-pulsed Nd:YAG laser as a new stimulus to induce BMP-2 expression in MC3T3-E1 osteoblasts. Lasers Surg Med 42:510\u2013518","journal-title":"Lasers Surg Med"},{"key":"2453_CR38","doi-asserted-by":"publisher","first-page":"375","DOI":"10.1007\/s10103-014-1651-z","volume":"30","author":"S Belletti","year":"2015","unstructured":"Belletti S, Uggeri J, Mergoni G et al (2015) Effects of 915 nm GaAs diode laser on mitochondria of human dermal fibroblasts: analysis with confocal microscopy. Lasers Med Sci 30:375\u2013381","journal-title":"Lasers Med Sci"},{"key":"2453_CR39","volume-title":"The laser therapy handbook","author":"J Tun\u00e9r","year":"2004","unstructured":"Tun\u00e9r J, Hode L (2004) The laser therapy handbook. Prima Books, Gr\u00e4ngesberg"}],"container-title":["Lasers in Medical Science"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s10103-018-2453-5\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s10103-018-2453-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s10103-018-2453-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,2,14]],"date-time":"2019-02-14T19:48:04Z","timestamp":1550173684000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s10103-018-2453-5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,2,15]]},"references-count":39,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2018,8]]}},"alternative-id":["2453"],"URL":"https:\/\/doi.org\/10.1007\/s10103-018-2453-5","relation":{},"ISSN":["0268-8921","1435-604X"],"issn-type":[{"value":"0268-8921","type":"print"},{"value":"1435-604X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,2,15]]},"assertion":[{"value":"21 October 2017","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 January 2018","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"15 February 2018","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with ethical standards"}},{"value":"The authors declare that they have no conflict of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and\/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}}]}}