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A total of 579 cycles with LGCEs from 2019 to 2022 was retrospectively reviewed. In 323 cycles, single LAH producing small holes (10 \u03bcm) was performed on LGCEs on day 4 (D4-LAH). In 256 cycles with persistent LGCEs despite D4-LAH, a repeat LAH procedure was performed on day 6 (Dual-LAH) with a bigger hole (30 \u03bcm). We compared day 7 blastocyst formation rate, usable blastocyst rate, and good grade blastocyst rate from these day 6 LGCEs between the two groups. Compared to the D4-LAH group, the Dual-LAH group had both higher day 7 blastocyst formation rate (9.4% vs. 3.0%, <jats:italic>p<\/jats:italic> &lt; 0.001) and higher day 7 usable blastocyst rates (7.4% vs. 2.1%, <jats:italic>p<\/jats:italic> &lt; 0.001). For persistent LGCEs despite single LAH, performing a repeat LAH on day 6 increased day 7 blastocyst formation rate.<\/jats:p>","DOI":"10.1007\/s10103-023-03898-9","type":"journal-article","created":{"date-parts":[[2023,9,30]],"date-time":"2023-09-30T04:02:06Z","timestamp":1696046526000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Dual laser-assisted hatching: an effective technique to salvage low-grade cleavage-stage embryos and harvest day 7 blastocysts"],"prefix":"10.1007","volume":"38","author":[{"given":"Weihai","family":"Xu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiqi","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4763-0419","authenticated-orcid":false,"given":"Shishi","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,9,30]]},"reference":[{"issue":"3","key":"3898_CR1","doi-asserted-by":"publisher","first-page":"CD001894","DOI":"10.1002\/14651858.CD001894","volume":"3","author":"L Lacey","year":"2021","unstructured":"Lacey L, Hassan S, Franik S, Seif MW, Akhtar MA (2021) Assisted hatching on assisted conception (in vitro fertilisation (IVF) and intracytoplasmic sperm injection (ICSI)). 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