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The incidence of drug-resistant strains of <jats:italic>C<\/jats:italic>. <jats:italic>albicans<\/jats:italic> is rising, necessitating the development of new antifungal drugs. Antimicrobial peptides (AMPs) have recently attracted attention due to their unique ability to evade the drug resistance of microorganisms. However, the mechanism of their activity has not yet been identified. The current study analyzed the mode of action of MAF-1A by confocal microscopy, scanning electron microscopy, fluorescent staining, flow cytometry, and qRT-PCR. The results indicate that MAF-1A disrupts the cell membrane of <jats:italic>C<\/jats:italic>. <jats:italic>albicans<\/jats:italic> and enters the cell where it binds and interacts with nucleic acids. qRT-PCR demonstrated that the expression of several sterol biosynthesis\u2013related genes in <jats:italic>C<\/jats:italic>. <jats:italic>albicans<\/jats:italic> was increased after MAF-1A treatment. 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The antifungal mechanism of MAF-1A is unique, and its identification has great research and clinical significance.<\/jats:p>","DOI":"10.1007\/s10123-021-00159-z","type":"journal-article","created":{"date-parts":[[2021,1,16]],"date-time":"2021-01-16T09:03:48Z","timestamp":1610787828000},"page":"233-242","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Antifungal activity of MAF-1A peptide against Candida albicans"],"prefix":"10.1007","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0307-9506","authenticated-orcid":false,"given":"Rong","family":"Cheng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fangfang","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongling","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zonggang","family":"Duan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianwei","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenhua","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,1,16]]},"reference":[{"key":"159_CR1","doi-asserted-by":"publisher","DOI":"10.1186\/s12905-018-0607-z","volume":"18","author":"A Bitew","year":"2018","unstructured":"Bitew A, Abebaw Y (2018) Vulvovaginal candidiasis: species distribution of Candida and their antifungal susceptibility pattern. 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