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We evaluated the possible correlation between <jats:italic>spd<\/jats:italic>C gene expression level and virulence as well as antibiotic resistance phenotypes in <jats:italic>S. aureus<\/jats:italic> clinical isolates. The antimicrobial susceptibility of <jats:italic>S. aureus<\/jats:italic> clinical isolates (<jats:italic>n<\/jats:italic>\u2009=\u2009100) was determined by the disk diffusion method. Vancomycin susceptibility was determined by the broth microdilution method. The level of the extracellular proteases and delta-hemolysin was evaluated by measuring the proteolysis and hemolysis zone diameters in skim milk and blood agar plates, respectively. Biofilm formation was assayed using the 96-well microtiter plate method. Most of the isolates (81%) were multidrug-resistant and about half of the isolates (49%) were methicillin-resistant <jats:italic>S. aureus<\/jats:italic>. Hemolysin, protease, and biofilm production were detectable in 79%, 71%, and 96% of the isolates. No significant correlation was detectable between the level of <jats:italic>spd<\/jats:italic>C gene expression and the activity of tested virulence factors or the antimicrobial resistance phenotype. Therefore, the role of SpdC protein as a virulence regulator in <jats:italic>S. aureus<\/jats:italic> needs further evaluation together with the determination of the predominant regulators for each virulence factor.<\/jats:p>","DOI":"10.1007\/s10123-022-00249-6","type":"journal-article","created":{"date-parts":[[2022,5,24]],"date-time":"2022-05-24T06:04:56Z","timestamp":1653372296000},"page":"649-659","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Effect of spdC gene expression on virulence and antibiotic resistance in clinical Staphylococcus aureus isolates"],"prefix":"10.1007","volume":"25","author":[{"given":"Mayada E.","family":"Bakr","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2505-4959","authenticated-orcid":false,"given":"Mona T.","family":"Kashef","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alaa El-Dien M. 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