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The presence of glycopeptide resistance genes and five virulence genes (<jats:italic>esp<\/jats:italic>, <jats:italic>gelE<\/jats:italic>, <jats:italic>asa1<\/jats:italic>, <jats:italic>hyl<\/jats:italic>, and <jats:italic>cylA<\/jats:italic>) was screened using two multiplex PCR. MLST was used to assess the clonality. Crystal violet staining was used to detect biofilms. Vancomycin resistance was detected in 30.1% of <jats:italic>Efm<\/jats:italic> and 2.8% of <jats:italic>Efs<\/jats:italic> isolates, respectively. All VRE strains carried the <jats:italic>vanA<\/jats:italic> gene. The <jats:italic>esp<\/jats:italic>, <jats:italic>gelE<\/jats:italic>, <jats:italic>asa1<\/jats:italic>, and <jats:italic>cylA<\/jats:italic> genes in 72 <jats:italic>Efs<\/jats:italic> strains were detected at 62.5%, 84.7%, 84.7%, and 69.4%, respectively. Among the 116 <jats:italic>Efm<\/jats:italic> isolates, 74.1% and 25.8% carried <jats:italic>esp<\/jats:italic> and <jats:italic>hyl<\/jats:italic>, respectively. The <jats:italic>esp<\/jats:italic> gene was significantly associated with vancomycin-resistant <jats:italic>Efm<\/jats:italic> (VREfm) compared to vancomycin-susceptible <jats:italic>Efm<\/jats:italic> (VSEfm). In total, 91.7% of <jats:italic>Efs<\/jats:italic> and 20.0% of <jats:italic>Efm<\/jats:italic> produced biofilms. Twenty-six STs were identified among the 72 <jats:italic>Efs<\/jats:italic> isolates, with ST4 (29.2%) being the predominant. In total, 116 <jats:italic>Efm<\/jats:italic> strains were grouped into 26 STs, with ST78 (46.6%) being the predominant. Both VREfm (41.7%) and VSEfm (48.8%) were dominant in ST78. There is no clear evidence suggesting that some STs are associated with vancomycin resistance or biofilm formation. Both <jats:italic>Efm<\/jats:italic> and <jats:italic>Efs<\/jats:italic> BSI isolates showed a polyclonal pattern with a dominant clone and many unique types, implying the coexistence of clonal dissemination and an influx of new clones. The horizontal transmission of resistance genes may play a more important role in VREfm prevalence than clonal expansion.<\/jats:p>","DOI":"10.1007\/s10123-023-00441-2","type":"journal-article","created":{"date-parts":[[2023,11,6]],"date-time":"2023-11-06T22:01:42Z","timestamp":1699308102000},"page":"929-939","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Molecular characterization and biofilm formation ability of Enterococcus faecium and Enterococcus faecalis bloodstream isolates from a Chinese tertiary hospital in Beijing"],"prefix":"10.1007","volume":"27","author":[{"given":"Jing-xian","family":"Yang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cun-wei","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fu-wei","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guo-wei","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,11,6]]},"reference":[{"key":"441_CR1","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1016\/j.jhin.2021.11.021","volume":"121","author":"O Abu-Lybdeh","year":"2022","unstructured":"Abu-Lybdeh O, Murik O, Oster Y et al (2022) Vancomycin-resistant enterococcus bacteraemia in an endemic region: clinical features and genomic analysis: a 12-year cohort. 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