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Therefore, this study aimed to analyze the volatile profile of three Gram-positive bacteria of clinical relevance to identify potential volatile biomarkers that allow their differentiation.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods and results<\/jats:title>\n                <jats:p><jats:italic>L. monocytogenes<\/jats:italic>, <jats:italic>S. aureus<\/jats:italic>, and <jats:italic>E. faecalis<\/jats:italic> clinical isolates were inoculated in a thioglycollate medium until grown. Then, VOCs were extracted by solid-phase microextraction, and the data obtained were subjected to multivariate analysis. According to our results, there was a high production of aldehydes in <jats:italic>E. faecalis<\/jats:italic>. In the case of alcohols, they only increased in <jats:italic>L. monocytogenes<\/jats:italic>, while ketones were produced significantly in all three bacteria, mainly due to acetoin. Acids were produced significantly in <jats:italic>E. faecalis<\/jats:italic> and <jats:italic>L. monocytogenes<\/jats:italic>.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>Potential biomarkers of <jats:italic>L. monocytogenes<\/jats:italic> could be 1-butanol and 2-methylbutanoic acid. In the case of <jats:italic>E. faecalis<\/jats:italic>, the VOC most related to its presence was nonanal. Lastly, potential biomarkers of <jats:italic>S. aureus<\/jats:italic> could be isoamyl butanoate and methionol, although some pyrazines have also been associated with this bacterium.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Significance and impact of the study<\/jats:title>\n                <jats:p>The identification of potential biomarkers of these clinically relevant bacteria could open the way for the diagnosis of these infections through the analysis of volatile compounds.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1007\/s10123-024-00511-z","type":"journal-article","created":{"date-parts":[[2024,3,21]],"date-time":"2024-03-21T12:02:07Z","timestamp":1711022527000},"page":"1737-1745","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Volatile biomarkers of Gram-positive bacteria of clinical relevance as a tool for infection diagnosis"],"prefix":"10.1007","volume":"27","author":[{"given":"Ricardo","family":"Rubio-S\u00e1nchez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esperanza","family":"Lepe-Balsalobre","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Ubeda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Antonio","family":"Lepe-Jim\u00e9nez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,3,21]]},"reference":[{"key":"511_CR1","doi-asserted-by":"crossref","unstructured":"Vila J, \u00c1lvarez-Mart\u00ednez MJ, Buesa J, Castillo J (2009) Diagn\u00f3stico microbiol\u00f3gico de las infecciones gastrointestinales. 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