{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T18:58:46Z","timestamp":1781636326096,"version":"3.54.5"},"reference-count":22,"publisher":"Springer Science and Business Media LLC","issue":"3","license":[{"start":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T00:00:00Z","timestamp":1771977600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T00:00:00Z","timestamp":1771977600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Erasmus+ Mobility Studentship","award":["DK KOBENHA 57"],"award-info":[{"award-number":["DK KOBENHA 57"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Int Microbiol"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Infections with Gram-negative bacteria that are resistant to multiple antibiotics are increasingly prevalent and challenging to treat. We sought to identify compounds with potential to reverse resistance to tetracycline and tobramycin in a multi-drug resistant isolate of\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    (NCTC 13400). A screen of 800 extracts of traditional herbs and medicines revealed that polar extracts of cloves (\n                    <jats:italic>Syzygium aromaticum<\/jats:italic>\n                    ) and Peony flowers (\n                    <jats:italic>Paeonia lactiflora<\/jats:italic>\n                    ) significantly increased sensitivity to both antibiotics in this strain. Fractionation of clove and peony extracts by high performance liquid chromatography revealed activity within the fractions comprising mainly phenolic acids and flavonoids, respectively. Sampling candidate compounds from these fractions revealed that while no tested compound enhanced the activity of tobramycin, myricetin significantly increased the sensitivity of this strain to tetracycline. Myricetin alone inhibited growth, but was not bactericidal and did not induce membrane permeability or inhibit Nile red efflux. In vitro MTT assays using mammalian HEK-293 cells revealed no significant toxicity of myricetin, or most of the other tested compounds, at up to 100 \u00b5M. The results suggest that the screening of plant secondary metabolites has potential for the identification of antibiotic resistance reversers with potentially favourable in vitro toxicity profiles.\n                  <\/jats:p>","DOI":"10.1007\/s10123-026-00798-0","type":"journal-article","created":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T08:36:37Z","timestamp":1772008597000},"page":"359-367","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Reversal of tetracycline resistance by clove and peony extracts in a multi-drug resistant Escherichia coli"],"prefix":"10.1007","volume":"29","author":[{"given":"Darko","family":"Jenic","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2978-346X","authenticated-orcid":false,"given":"Clett","family":"Erridge","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,2,25]]},"reference":[{"key":"798_CR1","first-page":"66","volume":"141","author":"M Abidov","year":"2006","unstructured":"Abidov M, Ramazanov A, Del Jimenez M, Chkhikvishvili I (2006) Effect of Blueberin on fasting glucose, C-reactive protein and plasma aminotransferases, in female volunteers with diabetes type 2: double-blind, placebo controlled clinical study. Georgian Med News 141:66\u201372","journal-title":"Georgian Med News"},{"key":"798_CR2","doi-asserted-by":"publisher","first-page":"15387","DOI":"10.3390\/ijms131115387","volume":"13","author":"J Akbar","year":"2012","unstructured":"Akbar J, Iqbal S, Batool F, Karim A, Chan KW (2012) Predicting retention times of naturally occurring phenolic compounds in reversed-phase liquid chromatography: a Quantitative Structure-Retention Relationship (QSRR) approach. Int J Mol Sci 13:15387\u201315400. https:\/\/doi.org\/10.3390\/ijms131115387","journal-title":"Int J Mol Sci"},{"key":"798_CR3","doi-asserted-by":"publisher","first-page":"1537","DOI":"10.3390\/ph15121537","volume":"15","author":"N \u010cutovi\u0107","year":"2022","unstructured":"\u010cutovi\u0107 N, Markovi\u0107 T, Kosti\u0107 M, Ga\u0161i\u0107 U, Priji\u0107 \u017d, Ren X, Luki\u0107 M, Bugarski B (2022) Chemical profile and skin-beneficial activities of the petal extracts of Paeonia tenuifolia L. from Serbia. 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