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As these particles enter the food chain, they have the potential to pose significant risks to human health. However, their effects on vital fish tissues, such as skeletal muscle, are not yet fully understood. In this study, we examined Nile tilapia (<jats:italic>Oreochromis niloticus<\/jats:italic>) from two distinct sites on the Nile River in Egypt: the Nile branch (Damietta branch) and Riah El-Towfiqi. Using Fourier Transform Infrared Spectroscopy (FTIR) and histological study, we confirmed the presence of MPs in both gastrointestinal and muscle tissues. We focused on understanding how MPs might affect fish muscle by investigating the expression of genes involved in muscle atrophy and hypertrophy using Real Time-PCR and histological alterations in muscle tissues of tilapia collected from the two studied sites in the four seasons. Our results revealed histological alterations in muscle tissues collected from the two sites studied in the four seasons. The expression levels of atrophy-related genes, <jats:italic>Atrogin-1<\/jats:italic> (<jats:italic>Fbxo32<\/jats:italic>), <jats:italic>Capn-1<\/jats:italic>, and the apoptosis marker <jats:italic>Caspase3a<\/jats:italic> (<jats:italic>Casp3a<\/jats:italic>), showed increased expression, especially during the summer at both sites. On the other hand, the hypertrophy-related gene <jats:italic>Igf-1<\/jats:italic> exhibited a significant decrease while, muscle stem cell genes (<jats:italic>Pax3<\/jats:italic>, <jats:italic>Pax7<\/jats:italic>) and muscle differentiation gene markers (<jats:italic>Myf5<\/jats:italic>, <jats:italic>Mrf6<\/jats:italic>) displayed seasonal upregulation, with heightened activity during winter and summer, depending on the location. Additionally, immune-related genes (<jats:italic>Ccr9<\/jats:italic>, <jats:italic>Irak4<\/jats:italic>, <jats:italic>Igl-1<\/jats:italic>, <jats:italic>Tlr1<\/jats:italic>) demonstrated notable seasonal changes, with a peak during summer at the Nile branch. These findings demonstrate that MPs can disrupt muscle integrity and immune function in fish, with implications for ecosystem health and potential risks to human food security.<\/jats:p>","DOI":"10.1007\/s10126-025-10481-6","type":"journal-article","created":{"date-parts":[[2025,6,27]],"date-time":"2025-06-27T00:04:47Z","timestamp":1750982687000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Effects of Microplastics on Gene Expression, Muscular Performance, and Immunological Responses in Nile Tilapia (Oreochromis niloticus): Seasonal and Habitat Variations"],"prefix":"10.1007","volume":"27","author":[{"given":"Noura M.","family":"Nabawy","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seham A.","family":"Ibrahim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nassr Allah","family":"Abd El-Hameid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omar I.","family":"Ghonemy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Walaa M.","family":"Shaalan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,6,27]]},"reference":[{"key":"10481_CR1","doi-asserted-by":"publisher","first-page":"80","DOI":"10.1016\/j.chemosphere.2018.04.076","volume":"205","author":"S Abbasi","year":"2018","unstructured":"Abbasi S, Soltani N, Keshavarzi B, Moore F, Turner A, Hassanaghaei M (2018) Microplastics in different tissues of fish and prawn from the Musa Estuary, Persian Gulf. 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