{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T07:51:18Z","timestamp":1782460278521,"version":"3.54.5"},"reference-count":27,"publisher":"Springer Science and Business Media LLC","issue":"3","license":[{"start":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T00:00:00Z","timestamp":1778716800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T00:00:00Z","timestamp":1778716800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2022MC171"],"award-info":[{"award-number":["ZR2022MC171"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Mar Biotechnol"],"published-print":{"date-parts":[[2026,6]]},"DOI":"10.1007\/s10126-026-10641-2","type":"journal-article","created":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T08:02:02Z","timestamp":1780128122000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Bath-Immersion Delivery of nanos-MO-Vivo During Embryogenesis Perturbs Gonadal Development in the Pacific Oyster (Crassostrea gigas)"],"prefix":"10.1007","volume":"28","author":[{"given":"Huihui","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongli","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Tao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,5,14]]},"reference":[{"key":"10641_CR1","doi-asserted-by":"publisher","first-page":"537","DOI":"10.1093\/genetics\/160.2.537","volume":"160","author":"J Andrews","year":"2002","unstructured":"Andrews J, Oliver B (2002) Sex Determination Signals Control ovo-B Transcription in Drosophila melanogaster Germ Cells. Genetics 160:537\u2013545. https:\/\/doi.org\/10.1093\/genetics\/160.2.537","journal-title":"Genetics"},{"key":"10641_CR2","doi-asserted-by":"publisher","first-page":"215","DOI":"10.1083\/jcb.201511061","volume":"214","author":"AV Cant\u00fa","year":"2016","unstructured":"Cant\u00fa AV, Altshuler-Keylin S, Laird DJ (2016) Discrete somatic niches coordinate proliferation and migration of primordial germ cells via Wnt signaling. J Cell Biol 214:215\u2013229. https:\/\/doi.org\/10.1083\/jcb.201511061","journal-title":"J Cell Biol"},{"key":"10641_CR3","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1016\/j.aquaculture.2010.02.030","volume":"303","author":"E Cotter","year":"2010","unstructured":"Cotter E, Malham SK, O\u2019Keeffe S et al (2010) Summer mortality of the Pacific oyster, Crassostrea gigas, in the Irish Sea: The influence of growth, biochemistry and gametogenesis. Aquaculture 303:8\u201321. https:\/\/doi.org\/10.1016\/j.aquaculture.2010.02.030","journal-title":"Aquaculture"},{"key":"10641_CR4","doi-asserted-by":"publisher","first-page":"1929","DOI":"10.1007\/s00018-018-2766-3","volume":"75","author":"E De Keuckelaere","year":"2018","unstructured":"De Keuckelaere E, Hulpiau P, Saeys Y et al (2018) Nanos genes and their role in development and beyond. Cell Mol Life Sci 75:1929\u20131946. https:\/\/doi.org\/10.1007\/s00018-018-2766-3","journal-title":"Cell Mol Life Sci"},{"key":"10641_CR5","doi-asserted-by":"publisher","first-page":"939","DOI":"10.1016\/j.fsi.2012.12.007","volume":"34","author":"Y Du","year":"2013","unstructured":"Du Y, Zhang L, Xu F et al (2013) Validation of housekeeping genes as internal controls for studying gene expression during Pacific oyster (Crassostrea gigas) development by quantitative real-time PCR. Fish Shellfish Immunol 34:939\u2013945. https:\/\/doi.org\/10.1016\/j.fsi.2012.12.007","journal-title":"Fish Shellfish Immunol"},{"key":"10641_CR6","doi-asserted-by":"publisher","first-page":"592","DOI":"10.1016\/j.bbrc.2004.06.009","volume":"320","author":"C Fabioux","year":"2004","unstructured":"Fabioux C, Huvet A, Lelong C et al (2004) Oyster vasa-like gene as a marker of the germline cell development in Crassostrea gigas. Biochem Biophys Res Commun 320:592\u2013598. https:\/\/doi.org\/10.1016\/j.bbrc.2004.06.009","journal-title":"Biochem Biophys Res Commun"},{"key":"10641_CR7","doi-asserted-by":"publisher","first-page":"329","DOI":"10.2983\/035.028.0215","volume":"28","author":"E G\u00f3mez-Robles","year":"2009","unstructured":"G\u00f3mez-Robles E, Saucedo PE (2009) Evaluation of Quality Indices of the Gonad and Somatic Tissues Involved in Reproduction of the Pearl Oyster Pinctada mazatlanica with Histochemistry and Digital Image Analysis. shre 28:329\u2013335. https:\/\/doi.org\/10.2983\/035.028.0215","journal-title":"shre"},{"key":"10641_CR9","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1007\/s10126-011-9421-4","volume":"14","author":"A Huvet","year":"2012","unstructured":"Huvet A, Fleury E, Corporeau C et al (2012) In Vivo RNA Interference of a Gonad-Specific Transforming Growth Factor-\u03b2 in the Pacific Oyster Crassostrea gigas. Mar Biotechnol 14:402\u2013410. https:\/\/doi.org\/10.1007\/s10126-011-9421-4","journal-title":"Mar Biotechnol"},{"key":"10641_CR8","doi-asserted-by":"publisher","first-page":"38","DOI":"10.16535\/j.cnki.dlhyxb.2023-120","volume":"39","author":"Y Hu","year":"2024","unstructured":"Hu Y, Leng J, Sun J et al (2024) Expression characteristics of CgLOX4 gene in larval development of Pacific oyster (Crassostrea gigas). Dalian Fish Univ 39:38\u201347. https:\/\/doi.org\/10.16535\/j.cnki.dlhyxb.2023-120","journal-title":"Dalian Fish Univ"},{"key":"10641_CR10","doi-asserted-by":"publisher","first-page":"796","DOI":"10.1111\/raq.12868","volume":"16","author":"K Jiang","year":"2024","unstructured":"Jiang K, Chen C, Jiang G et al (2024) Genetic improvement of oysters: Current status, challenges, and prospects. Reviews Aquaculture 16:796\u2013817. https:\/\/doi.org\/10.1111\/raq.12868","journal-title":"Reviews Aquaculture"},{"key":"10641_CR11","doi-asserted-by":"publisher","first-page":"781","DOI":"10.1038\/nrg3294","volume":"13","author":"BJ Lesch","year":"2012","unstructured":"Lesch BJ, Page DC (2012) Genetics of germ cell development. Nat Rev Genet 13:781\u2013794. https:\/\/doi.org\/10.1038\/nrg3294","journal-title":"Nat Rev Genet"},{"key":"10641_CR12","doi-asserted-by":"publisher","first-page":"591","DOI":"10.1534\/genetics.114.163667","volume":"197","author":"M Li","year":"2014","unstructured":"Li M, Yang H, Zhao J et al (2014) Efficient and Heritable Gene Targeting in Tilapia by CRISPR\/Cas9. Genetics 197:591\u2013599. https:\/\/doi.org\/10.1534\/genetics.114.163667","journal-title":"Genetics"},{"key":"10641_CR13","doi-asserted-by":"publisher","first-page":"643","DOI":"10.1007\/s12562-011-0369-0","volume":"77","author":"Q Li","year":"2011","unstructured":"Li Q, Wang Q, Liu S, Kong L (2011) Selection response and realized heritability for growth in three stocks of the Pacific oyster Crassostrea gigas. Fish Sci 77:643\u2013648. https:\/\/doi.org\/10.1007\/s12562-011-0369-0","journal-title":"Fish Sci"},{"key":"10641_CR14","doi-asserted-by":"publisher","first-page":"D1075","DOI":"10.1093\/nar\/gkae1026","volume":"53","author":"F Liu","year":"2025","unstructured":"Liu F, Cai B, Lian S et al (2025) MolluscDB 2.0: a comprehensive functional and evolutionary genomics database for over 1400 molluscan species. Nucleic Acids Res 53:D1075\u2013D1086. https:\/\/doi.org\/10.1093\/nar\/gkae1026","journal-title":"Nucleic Acids Res"},{"key":"10641_CR15","doi-asserted-by":"publisher","first-page":"253","DOI":"10.1007\/s00427-015-0511-6","volume":"225","author":"G Liu","year":"2015","unstructured":"Liu G, Huan P, Liu B (2015) A GATA2\/3 gene potentially involved in larval shell formation of the Pacific oyster Crassostrea gigas. Dev Genes Evol 225:253\u2013257. https:\/\/doi.org\/10.1007\/s00427-015-0511-6","journal-title":"Dev Genes Evol"},{"key":"10641_CR16","doi-asserted-by":"publisher","first-page":"826","DOI":"10.2108\/zsj.30.826","volume":"30","author":"T Matsumoto","year":"2013","unstructured":"Matsumoto T, Masaoka T, Fujiwara A et al (2013) Reproduction-Related Genes in the Pearl Oyster Genome. Zool Sci 30:826. https:\/\/doi.org\/10.2108\/zsj.30.826","journal-title":"Zool Sci"},{"key":"10641_CR17","doi-asserted-by":"publisher","first-page":"286","DOI":"10.2108\/zs210123","volume":"39","author":"T Nishimura","year":"2022","unstructured":"Nishimura T, Tanaka M (2022) Zygotic nanos3 Mutant Medaka (Oryzias latipes) Displays Gradual Loss of Germ Cells and Precocious Spermatogenesis During Gonadal Development. jzoo 39:286\u2013292. https:\/\/doi.org\/10.2108\/zs210123","journal-title":"jzoo"},{"key":"10641_CR18","doi-asserted-by":"publisher","unstructured":"Oikawa M, Kojima H, Kobayashi H et al (2025) Transcription factor-mediated germ cell induction in rats reveals ETV4 cooperates with germline specifiers. Stem Cell Rep 20. https:\/\/doi.org\/10.1016\/j.stemcr.2025.102599","DOI":"10.1016\/j.stemcr.2025.102599"},{"key":"10641_CR19","doi-asserted-by":"publisher","first-page":"457","DOI":"10.2983\/0730-8000","volume":"24","author":"A Parra","year":"2005","unstructured":"Parra A, Garc\u00cda O, Isla F (2005) Seasonal variations on the biochemical composition and lipid classed of the gonadal and storage tissue of Crassostrea gigas (Thunberg, 1794). Shellfish Res 24:457\u2013467. https:\/\/doi.org\/10.2983\/0730-8000","journal-title":"Shellfish Res"},{"key":"10641_CR20","doi-asserted-by":"publisher","first-page":"e0134280","DOI":"10.1371\/journal.pone.0134280","volume":"10","author":"Y Tong","year":"2015","unstructured":"Tong Y, Zhang Y, Huang J et al (2015) Transcriptomics Analysis of Crassostrea hongkongensis for the Discovery of Reproduction-Related Genes. PLoS ONE 10:e0134280. https:\/\/doi.org\/10.1371\/journal.pone.0134280","journal-title":"PLoS ONE"},{"key":"10641_CR21","doi-asserted-by":"publisher","first-page":"16582","DOI":"10.1073\/pnas.1112561108","volume":"108","author":"D Wesolowski","year":"2011","unstructured":"Wesolowski D, Tae HS, Gandotra N et al (2011) Basic peptide-morpholino oligomer conjugate that is very effective in killing bacteria by gene-specific and nonspecific modes. Proc Natl Acad Sci USA 108:16582\u201316587. https:\/\/doi.org\/10.1073\/pnas.1112561108","journal-title":"Proc Natl Acad Sci USA"},{"key":"10641_CR22","doi-asserted-by":"publisher","first-page":"15822","DOI":"10.1038\/srep15822","volume":"5","author":"TT Wong","year":"2015","unstructured":"Wong TT, Zohar Y (2015) Production of reproductively sterile fish by a non-transgenic gene silencing technology. Sci Rep 5:15822. https:\/\/doi.org\/10.1038\/srep15822","journal-title":"Sci Rep"},{"key":"10641_CR23","doi-asserted-by":"publisher","first-page":"742261","DOI":"10.1016\/j.aquaculture.2025.742261","volume":"600","author":"L Xu","year":"2025","unstructured":"Xu L, Small JM, Hood SM et al (2025) Morpholino oligomer delivery via bath immersion for use in reverse genetic studies on the early development of eastern oysters (Crassostrea virginica). Aquaculture 600:742261. https:\/\/doi.org\/10.1016\/j.aquaculture.2025.742261","journal-title":"Aquaculture"},{"key":"10641_CR24","doi-asserted-by":"publisher","first-page":"220","DOI":"10.1111\/raq.12712","volume":"15","author":"L Xu","year":"2023","unstructured":"Xu L, Zhao M, Ryu JH et al (2023) Reproductive sterility in aquaculture: A review of induction methods and an emerging approach with application to Pacific Northwest finfish species. Reviews Aquaculture 15:220\u2013241. https:\/\/doi.org\/10.1111\/raq.12712","journal-title":"Reviews Aquaculture"},{"key":"10641_CR26","first-page":"100486","volume":"18","author":"R Xu","year":"2020","unstructured":"Xu R, Li Q, Yu H (2020) Expression pattern of Piwi-like gene implies the potential role in germline development in the Pacific oyster Crossosrea gigas. Aquacult Rep 18:100486. https:\/\/www.sciencedirect.com\/science\/article\/pii\/S2352513420305767","journal-title":"Aquacult Rep"},{"key":"10641_CR25","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1016\/j.gene.2018.04.021","volume":"663","author":"R Xu","year":"2018","unstructured":"Xu R, Li Q, Yu H, Kong L (2018) Oocyte maturation and origin of the germline as revealed by the expression of Nanos-like in the Pacific oyster Crassostrea gigas. Gene 663:41\u201350. https:\/\/doi.org\/10.1016\/j.gene.2018.04.021","journal-title":"Gene"},{"issue":"3","key":"10641_CR27","doi-asserted-by":"publisher","first-page":"301","DOI":"10.1007\/s10126-019-09885-y","volume":"21","author":"H Yu","year":"2019","unstructured":"Yu H, Li H, Li Q (2019) Targeted gene disruption in Pacific oyster based on CRISPR\/Cas9 ribonucleoprotein complexes. Mar Biotechnol 21(3):301\u2013309. https:\/\/doi.org\/10.1007\/s10126-019-09885-y","journal-title":"Mar Biotechnol"}],"container-title":["Marine Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10126-026-10641-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10126-026-10641-2","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10126-026-10641-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T07:41:34Z","timestamp":1782459694000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10126-026-10641-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,14]]},"references-count":27,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["10641"],"URL":"https:\/\/doi.org\/10.1007\/s10126-026-10641-2","relation":{},"ISSN":["1436-2228","1436-2236"],"issn-type":[{"value":"1436-2228","type":"print"},{"value":"1436-2236","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,14]]},"assertion":[{"value":"16 January 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 May 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 May 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing Interests"}}],"article-number":"91"}}