{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T18:36:05Z","timestamp":1787337365872,"version":"build-2736575974"},"reference-count":39,"publisher":"Springer Science and Business Media LLC","issue":"4","license":[{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100012428","name":"Central Public-interest Scientific Institution Basal Research Fund, CAFS","doi-asserted-by":"crossref","award":["2023TD20"],"award-info":[{"award-number":["2023TD20"]}],"id":[{"id":"10.13039\/501100012428","id-type":"DOI","asserted-by":"crossref"}]},{"name":"STI 2030-Major projects","award":["2023ZD0405503"],"award-info":[{"award-number":["2023ZD0405503"]}]},{"name":"the National Key R&D Program of China","award":["2022YFD2400101"],"award-info":[{"award-number":["2022YFD2400101"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32230107"],"award-info":[{"award-number":["32230107"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"the Key Research and Development Project of Shandong Province","award":["2023ZLYS02"],"award-info":[{"award-number":["2023ZLYS02"]}]},{"name":"Shandong Taishan Scholar Climbing Project"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Mar Biotechnol"],"published-print":{"date-parts":[[2026,8]]},"DOI":"10.1007\/s10126-026-10682-7","type":"journal-article","created":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T08:23:51Z","timestamp":1785745431000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Reproducible Electroporation Strategy for CRISPR-Cas9 RNP and mRNA Delivery in Fish Embryos"],"prefix":"10.1007","volume":"28","author":[{"given":"Yuanri","family":"Hu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Fang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongkai","family":"Cui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Songlin","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,8,3]]},"reference":[{"key":"10682_CR1","doi-asserted-by":"publisher","first-page":"362","DOI":"10.1242\/bio.20148177","volume":"3","author":"S Ansai","year":"2014","unstructured":"Ansai S, Kinoshita M (2014) Targeted mutagenesis using CRISPR\/Cas system in medaka. Biol Open 3:362\u2013371. https:\/\/doi.org\/10.1242\/bio.20148177","journal-title":"Biol Open"},{"key":"10682_CR2","doi-asserted-by":"publisher","first-page":"142","DOI":"10.1101\/gr.161638.113","volume":"24","author":"TO Auer","year":"2014","unstructured":"Auer TO, Duroure K, De Cian A, Concordet JP, Del Bene F (2014) Highly efficient CRISPR\/Cas9-mediated knock-in in zebrafish by homology-independent DNA repair. Genome Res 24:142\u2013153. https:\/\/doi.org\/10.1101\/gr.161638.113","journal-title":"Genome Res"},{"key":"10682_CR3","doi-asserted-by":"publisher","first-page":"D18","DOI":"10.1093\/nar\/gkad1078","volume":"52","author":"YM Bao","year":"2024","unstructured":"Bao YM et al (2024) Database Resources of the National Genomics Data Center, China National Center for Bioinformation in 2024. Nucleic Acids Res 52:D18\u2013D32. https:\/\/doi.org\/10.1093\/nar\/gkad1078","journal-title":"Nucleic Acids Res"},{"key":"10682_CR4","doi-asserted-by":"publisher","first-page":"qzae047","DOI":"10.1093\/gpbjnl\/qzae047","volume":"22","author":"CF Bu","year":"2024","unstructured":"Bu CF, Zheng XC, Zhao XT, Xu TY, Bai X, Jia YK, Chen ML, Hao LL, Xiao JF, Zhang Z, Zhao WM, Tang BX, Bao YM (2024) GenBase: A Nucleotide Sequence Database. Genom Proteom Bioinf 22:qzae047. https:\/\/doi.org\/10.1093\/gpbjnl\/qzae047","journal-title":"Genom Proteom Bioinf"},{"key":"10682_CR5","doi-asserted-by":"publisher","first-page":"2025","DOI":"10.1242\/dev.134809","volume":"143","author":"A Burger","year":"2016","unstructured":"Burger A, Lindsay H, Felker A, Hess C, Anders C, Chiavacci E, Zaugg J, Weber LM, Catena R, Jinek M, Robinson MD, Mosimann C (2016) Maximizing mutagenesis with solubilized CRISPR-Cas9 ribonucleoprotein complexes. Development 143:2025\u20132037. https:\/\/doi.org\/10.1242\/dev.134809","journal-title":"Development"},{"key":"10682_CR6","doi-asserted-by":"publisher","first-page":"1432","DOI":"10.1021\/acs.analchem.1c04795","volume":"94","author":"J Camperi","year":"2022","unstructured":"Camperi J, Moshref M, Dai L, Lee HY (2022) Physicochemical and Functional Characterization of Differential CRISPR-Cas9 Ribonucleoprotein Complexes. Anal Chem 94:1432\u20131440. https:\/\/doi.org\/10.1021\/acs.analchem.1c04795","journal-title":"Anal Chem"},{"key":"10682_CR7","doi-asserted-by":"publisher","first-page":"7899","DOI":"10.1073\/pnas.1818553116","volume":"116","author":"YH Cao","year":"2019","unstructured":"Cao YH, Ma EB, Cestellos-Blanco S, Zhang B, Qiu RY, Su YD, Doudna JA, Yang PD (2019) Nontoxic nanopore electroporation for effective intracellular delivery of biological macromolecules. PNAS 116:7899\u20137904. https:\/\/doi.org\/10.1073\/pnas.1818553116","journal-title":"PNAS"},{"key":"10682_CR8","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1016\/j.ymeth.2005.12.009","volume":"39","author":"GA Cerda","year":"2006","unstructured":"Cerda GA, Thomas JE, Allende ML, Karlstrom RO, Palma V (2006) Electroporation of DNA, RNA, and morpholinos into zebrafish embryos. Methods 39:207\u2013211. https:\/\/doi.org\/10.1016\/j.ymeth.2005.12.009","journal-title":"Methods"},{"key":"10682_CR9","doi-asserted-by":"publisher","first-page":"e2012229118","DOI":"10.1073\/pnas.2012229118","volume":"118","author":"ZW Chen","year":"2021","unstructured":"Chen ZW, Cao YH, Lin CW, Alvarez S, Oh D, Yang PD, Groves JT (2021) Nanopore-mediated protein delivery enabling three-color single-molecule tracking in living cells. PNAS 118:e2012229118. https:\/\/doi.org\/10.1073\/pnas.2012229118","journal-title":"PNAS"},{"key":"10682_CR10","doi-asserted-by":"publisher","first-page":"543","DOI":"10.1007\/s10616-013-9608-9","volume":"66","author":"G Covello","year":"2014","unstructured":"Covello G, Siva K, Adami V, Denti MA (2014) An electroporation protocol for efficient DNA transfection in PC12 cells. Cytotechnology 66:543\u2013553. https:\/\/doi.org\/10.1007\/s10616-013-9608-9","journal-title":"Cytotechnology"},{"key":"10682_CR11","doi-asserted-by":"publisher","first-page":"42213","DOI":"10.1038\/srep42213","volume":"70","author":"ZK Cui","year":"2013","unstructured":"Cui ZK, Liu Y, Wang WW, Wang Q, Zhang N, Lin F, Wang N, Shao CW, Dong ZD, Li YZ, Yang YM, Hu MZ, Li HL, Gao FT, Wei ZF, Meng L, Liu Y, Wei M, Zhu Y, Guo H, Cheng CHK, Schartl M, Chen SL (2013) Genome editing reveals dmrt1 as an essential male sex-determining gene in Chinese tongue sole (Cynoglossus semilaevis). Sci Rep 7:42213. https:\/\/doi.org\/10.1038\/srep42213","journal-title":"Sci Rep"},{"key":"10682_CR12","doi-asserted-by":"publisher","first-page":"106853","DOI":"10.1016\/j.mtcomm.2023.106853","volume":"36","author":"F Guo","year":"2023","unstructured":"Guo F, Nie XW, Hong J, Zhang YP, Sun JG, Zhang Y (2023) Influence of Joule heating during single-cell electroporation simulation under IRE and H-FIRE pulses. Mater Today Commun 36:106853. https:\/\/doi.org\/10.1016\/j.mtcomm.2023.106853","journal-title":"Mater Today Commun"},{"key":"10682_CR13","doi-asserted-by":"publisher","first-page":"e0134755","DOI":"10.1371\/journal.pone.0134755","volume":"10","author":"N Hachicho","year":"2015","unstructured":"Hachicho N, Reithel S, Miltner A, Heipieper HJ, K\u00fcster E, Luckenbach T (2015) Body Mass Parameters, Lipid Profiles and Protein Contents of Zebrafish Embryos and Effects of 2,4-Dinitrophenol Exposure. PLoS ONE 10:e0134755. https:\/\/doi.org\/10.1371\/journal.pone.0134755","journal-title":"PLoS ONE"},{"key":"10682_CR14","doi-asserted-by":"publisher","first-page":"761","DOI":"10.1007\/s10544-007-9087-x","volume":"9","author":"KS Huang","year":"2007","unstructured":"Huang KS, Lin YC, Su KC, Chen HY (2007) An electroporation microchip system for the transfection of zebrafish embryos using quantum dots and GFP genes for evaluation. Biomed Microdevices 9:761\u2013768. https:\/\/doi.org\/10.1007\/s10544-007-9087-x","journal-title":"Biomed Microdevices"},{"key":"10682_CR15","doi-asserted-by":"publisher","first-page":"13904","DOI":"10.1073\/pnas.1308335110","volume":"110","author":"LE Jao","year":"2013","unstructured":"Jao LE, Wente SR, Chen WB (2013) Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system. PNAS 110:13904\u201313909. https:\/\/doi.org\/10.1073\/pnas.1308335110","journal-title":"PNAS"},{"key":"10682_CR16","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1186\/1472-6750-13-53","volume":"13","author":"G Jung","year":"2013","unstructured":"Jung G, Hug M, Halter C, Friesenhengst A, Walzer J, Czerny T (2013) Diffusion of small molecules into medaka embryos improved by electroporation. BMC Biotechnol 13:53. https:\/\/doi.org\/10.1186\/1472-6750-13-53","journal-title":"BMC Biotechnol"},{"key":"10682_CR17","doi-asserted-by":"publisher","first-page":"253","DOI":"10.1002\/aja.1002030302","volume":"203","author":"CB Kimmel","year":"1955","unstructured":"Kimmel CB, Ballard WW, Kimmel SR, Ullmann B, Schilling TF (1995) Stages of embryonic-development of the zebrafish. Dev Dynam 203:253\u2013310. https:\/\/doi.org\/10.1002\/aja.1002030302","journal-title":"Dev Dynam"},{"key":"10682_CR18","doi-asserted-by":"publisher","first-page":"481","DOI":"10.1038\/nature14592","volume":"523","author":"BP Kleinstiver","year":"2015","unstructured":"Kleinstiver BP, Prew MS, Tsai SQ, Topkar VV, Nguyen NT, Zheng Z, Gonzales APW, Li Z, Peterson RT, Yeh JJ, Aryee MJ, Joung JK (2015) Engineered CRISPR-Cas9 nucleases with altered PAM specificities. Nature 523:481\u2013249. https:\/\/doi.org\/10.1038\/nature14592","journal-title":"Nature"},{"key":"10682_CR19","doi-asserted-by":"publisher","first-page":"e0333369","DOI":"10.1371\/journal.pone.0333369","volume":"20","author":"HJ Lee","year":"2025","unstructured":"Lee HJ, Lee HK, Lee SW, Son YW, Shin JN, Kim S (2025) Automation in microinjection for zebrafish pericardial space with image-based motion control and batch agarose microplate. PLoS ONE 20:e0333369. https:\/\/doi.org\/10.1371\/journal.pone.0333369","journal-title":"PLoS ONE"},{"key":"10682_CR20","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1021\/nn700048y","volume":"1","author":"KJ Lee","year":"2007","unstructured":"Lee KJ, Nallathamby PD, Browning LM, Osgood CJ, Xu XHN (2007) In vivo imaging of transport and biocompatibility of single silver nanoparticles in early development of zebrafish embryos. ACS Nano 1:133\u2013143. https:\/\/doi.org\/10.1021\/nn700048y","journal-title":"ACS Nano"},{"key":"10682_CR21","doi-asserted-by":"publisher","first-page":"353","DOI":"10.1038\/nrg2091","volume":"8","author":"GJ Lieschke","year":"2007","unstructured":"Lieschke GJ, Currie PD (2007) Animal models of human disease: zebrafish swim into view. Nat Rev Genet 8:353\u2013367. https:\/\/doi.org\/10.1038\/nrg2091","journal-title":"Nat Rev Genet"},{"key":"10682_CR22","doi-asserted-by":"publisher","first-page":"50015","DOI":"10.1021\/acsami.3c10939","volume":"15","author":"J Liu","year":"2023","unstructured":"Liu J, Jiang J, He MY, Chen JY, Huang S, Liu ZJ, Yao CJ, Chen HJ, Xie X, Wang J (2023) Nanopore Electroporation Device for DNA Transfection into Various Spreading and Nonadherent Cell Types. ACS Appl Mater Interfaces 15:50015\u201350033. https:\/\/doi.org\/10.1021\/acsami.3c10939","journal-title":"ACS Appl Mater Interfaces"},{"key":"10682_CR23","doi-asserted-by":"publisher","first-page":"66","DOI":"10.1177\/2211068211432197","volume":"17","author":"D Mandrell","year":"2012","unstructured":"Mandrell D, Truong L, Jephson C, Sarker MR, Moore A, Lang C, Simonich MT, Tanguay RL (2012) Automated Zebrafish Chorion Removal and Single Embryo Placement: Optimizing Throughput of Zebrafish Developmental Toxicity Screens. Jala 17:66\u201374. https:\/\/doi.org\/10.1177\/2211068211432197","journal-title":"Jala"},{"key":"10682_CR24","doi-asserted-by":"publisher","first-page":"447","DOI":"10.1017\/S0967199414000045","volume":"23","author":"M Martinez-Sales","year":"2015","unstructured":"Martinez-Sales M, Garcia-Ximenez F, Espinos FJ (2015) Zebrafish (Danio rerio) as a possible bioindicator of epigenetic factors present in drinking water that may affect reproductive function: is chorion an issue? Zygote 23:447\u2013452. https:\/\/doi.org\/10.1017\/S0967199414000045","journal-title":"Zygote"},{"key":"10682_CR25","doi-asserted-by":"publisher","first-page":"740279","DOI":"10.1016\/j.aquaculture.2023.740279","volume":"579","author":"A Mokrani","year":"2024","unstructured":"Mokrani A, Liu SK (2024) Harnessing CRISPR\/Cas9 system to improve economic traits in aquaculture species. Aquaculture 579:740279. https:\/\/doi.org\/10.1016\/j.aquaculture.2023.740279","journal-title":"Aquaculture"},{"key":"10682_CR26","doi-asserted-by":"publisher","first-page":"118","DOI":"10.1002\/mrd.22142","volume":"80","author":"K Nagasawa","year":"2013","unstructured":"Nagasawa K, Fernandes JMO, Yoshizaki G, Miwa M, Babiak I (2013) Identification and migration of primordial germ cells in Atlantic salmon, Salmo salar: Characterization of Vasa, Dead End, and Lymphocyte antigen 75 genes. Mol Reprod Dev 80:118\u2013131. https:\/\/doi.org\/10.1002\/mrd.22142","journal-title":"Mol Reprod Dev"},{"key":"10682_CR27","doi-asserted-by":"publisher","first-page":"44","DOI":"10.1038\/s41587-019-0325-6","volume":"38","author":"DN Nguyen","year":"2020","unstructured":"Nguyen DN, Roth TL, Li PJ, Chen PA, Apathy R, Mamedov MR, Vo LT, Tobin VR, Goodman D, Shifrut E, Bluestone JA, Puck JM, Szoka FC, Marson A (2020) Polymer-stabilized Cas9 nanoparticles and modified repair templates increase genome editing efficiency. Nat Biotechnol 38:44. https:\/\/doi.org\/10.1038\/s41587-019-0325-6","journal-title":"Nat Biotechnol"},{"key":"10682_CR28","doi-asserted-by":"publisher","first-page":"114453","DOI":"10.1016\/j.ygcen.2024.114453","volume":"349","author":"M Rezanujjaman","year":"2024","unstructured":"Rezanujjaman M, Pachoensuk T, Hossain MF, Jyoti MMS, Rana MR, Tsutsumi E, Mouri T, Susilo MB, Wanlada K, Yamamoto C, Ali MH, Tokumoto T (2024) Zebrafish prss59.1 is involved in chorion development. Gen Comp Endocr 349:114453. https:\/\/doi.org\/10.1016\/j.ygcen.2024.114453","journal-title":"Gen Comp Endocr"},{"key":"10682_CR29","doi-asserted-by":"publisher","first-page":"103976","DOI":"10.1016\/j.compbiomed.2020.103976","volume":"125","author":"F Sadak","year":"2020","unstructured":"Sadak F, Saadat M, Hajiyavand AM (2020) Real-time deep learning-based image recognition for applications in automated positioning and injection of biological cells. Comput Biol Med 125:103976. https:\/\/doi.org\/10.1016\/j.compbiomed.2020.103976","journal-title":"Comput Biol Med"},{"key":"10682_CR30","doi-asserted-by":"publisher","first-page":"255","DOI":"10.3390\/ijms25074104","volume":"30","author":"DA Seli","year":"2024","unstructured":"Seli DA, Prendergast A, Ergun Y, Tyagi A, Taylor HS (2024) High NaCl Concentrations in Water Are Associated with Developmental Abnormalities and Altered Gene Expression in Zebrafish. Int J Mol Sci 30:255. https:\/\/doi.org\/10.3390\/ijms25074104","journal-title":"Int J Mol Sci"},{"key":"10682_CR31","doi-asserted-by":"publisher","first-page":"7409","DOI":"10.1021\/acs.chemrev.7b00678","volume":"118","author":"MP Stewart","year":"2018","unstructured":"Stewart MP, Langer R, Jensen KF (2018) Intracellular Delivery by Membrane Disruption: Mechanisms, Strategies, and Concepts. Chem Rev 118:7409\u20137531. https:\/\/doi.org\/10.1021\/acs.chemrev.7b00678","journal-title":"Chem Rev"},{"key":"10682_CR32","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1007\/s00542-020-04891-w","volume":"27","author":"LY Su","year":"2021","unstructured":"Su LY, Zhang HP, Wei HM, Zhang Z, Yu YQ, Si GN, Zhang XP (2021) Macro-to-micro positioning and auto focusing for fully automated single cell microinjection. Microsyst Technol 27:11\u201321. https:\/\/doi.org\/10.1007\/s00542-020-04891-w","journal-title":"Microsyst Technol"},{"key":"10682_CR33","doi-asserted-by":"publisher","first-page":"2778","DOI":"10.1016\/j.bbrc.2018.08.039","volume":"503","author":"K Takahashi","year":"2018","unstructured":"Takahashi K, Ishii K, Yamashita M (2018) Staufen1, Kinesin1 and microtubule function in cyclin B1 mRNA transport to the animal polar cytoplasm of zebrafish oocytes. Biochem Bioph Res Co 503:2778\u20132783. https:\/\/doi.org\/10.1016\/j.bbrc.2018.08.039","journal-title":"Biochem Bioph Res Co"},{"key":"10682_CR34","doi-asserted-by":"publisher","first-page":"40","DOI":"10.1016\/j.aquatox.2017.06.017","volume":"190","author":"M Van Pomeren","year":"2017","unstructured":"Van Pomeren M, Brun NR, Peijnenburg WJGM, Vijver MG (2017) Exploring uptake and biodistribution of polystyrene (nano)particles in zebrafish embryos at different developmental stages. Aquat Toxicol 190:40\u201345. https:\/\/doi.org\/10.1016\/j.aquatox.2017.06.017","journal-title":"Aquat Toxicol"},{"key":"10682_CR35","doi-asserted-by":"publisher","first-page":"2357","DOI":"10.1038\/nprot.2016.141","volume":"11","author":"GK Varshney","year":"2016","unstructured":"Varshney GK, Carrington B, Pei W, Bishop K, Chen Z, Fan C, Xu L, Jones M, LaFave MC, Ledin J, Sood R, Burgess SM (2016) A high-throughput functional genomics workflow based on CRISPR\/Cas9-mediated targeted mutagenesis in zebrafish. Nat Protoc 11:2357\u20132375. https:\/\/doi.org\/10.1038\/nprot.2016.141","journal-title":"Nat Protoc"},{"key":"10682_CR36","doi-asserted-by":"publisher","first-page":"1297","DOI":"10.2478\/s11756-020-00677-7","volume":"76","author":"L Wang","year":"2021","unstructured":"Wang L, Tan XG, Wu ZH, Wang LJ, Jiao S, Zou YX, Ji GL, You F (2021) Targeted mutagenesis in the olive flounder (Paralichthys olivaceus) using the CRISPR\/Cas9 system with electroporation. Biologia 76:1297\u20131304. https:\/\/doi.org\/10.2478\/s11756-020-00677-7","journal-title":"Biologia"},{"key":"10682_CR37","doi-asserted-by":"publisher","first-page":"644","DOI":"10.1007\/s10126-020-09985-0","volume":"22","author":"CQ Zhang","year":"2020","unstructured":"Zhang CQ, Ren ZH, Gong ZY (2020) Transgenic Expression and Genome Editing by Electroporation of Zebrafish Embryos. Mar Biotechnol 22:644\u2013650. https:\/\/doi.org\/10.1007\/s10126-020-09985-0","journal-title":"Mar Biotechnol"},{"key":"10682_CR38","doi-asserted-by":"publisher","first-page":"846","DOI":"10.1016\/j.theriogenology.2014.12.021","volume":"84","author":"H Zhang","year":"2015","unstructured":"Zhang H, Wang LJ, Li WZ, Mao QF, Wang YS, Li Q, Hua S, Zhang Y (2015) A simple and efficient method to transfect small interference RNA into bovine SCNT embryos. Theriogenology 84:846\u2013852. https:\/\/doi.org\/10.1016\/j.theriogenology.2014.12.021","journal-title":"Theriogenology"},{"key":"10682_CR39","doi-asserted-by":"publisher","first-page":"7","DOI":"10.3390\/mi10010007","volume":"10","author":"YL Zhao","year":"2019","unstructured":"Zhao YL, Sun H, Sha XP, Gu LJ, Zhan ZK, Li WJ (2019) A Review of Automated Microinjection of Zebrafish Embryos. Micromachines 10:7. https:\/\/doi.org\/10.3390\/mi10010007","journal-title":"Micromachines"}],"container-title":["Marine Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10126-026-10682-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10126-026-10682-7","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10126-026-10682-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T18:02:45Z","timestamp":1787335365000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10126-026-10682-7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8]]},"references-count":39,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10682"],"URL":"https:\/\/doi.org\/10.1007\/s10126-026-10682-7","relation":{},"ISSN":["1436-2228","1436-2236"],"issn-type":[{"value":"1436-2228","type":"print"},{"value":"1436-2236","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8]]},"assertion":[{"value":"3 April 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"25 July 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"3 August 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors confirm that there are no known conflicts of interest, financial or otherwise, that could have affected the work presented herein.","order":1,"name":"Ethics","label":"Competing interest","group":{"name":"EthicsHeading","label":"Declarations"}}],"article-number":"130"}}