{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T18:36:44Z","timestamp":1793299004076,"version":"4.5.0"},"reference-count":75,"publisher":"Springer Science and Business Media LLC","issue":"5","license":[{"start":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T00:00:00Z","timestamp":1789603200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T00:00:00Z","timestamp":1789603200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["RS-2026-25479595"],"award-info":[{"award-number":["RS-2026-25479595"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Mar Biotechnol"],"published-print":{"date-parts":[[2026,10]]},"DOI":"10.1007\/s10126-026-10707-1","type":"journal-article","created":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T11:01:05Z","timestamp":1789642865000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparative Genomics-Guided Epitope Prioritization and in Silico Design of a Multi-Epitope DNA Vaccine Candidate Against Megalocytivirus pagrus 1"],"prefix":"10.1007","volume":"28","author":[{"given":"Sung-Bin","family":"Moon","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min-Young","family":"Sohn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gyoungsik","family":"Kang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"HyeongJin","family":"Roh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoonhang","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min Jae","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwang Il","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seong Don","family":"Hwang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chan-Il","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyung-Ho","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,9,17]]},"reference":[{"issue":"3","key":"10707_CR1","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1007\/s10126-026-10621-6","volume":"28","author":"N Abdellaoui","year":"2026","unstructured":"Abdellaoui N, Lim JW, Han HJ, Cho MY, Kim MS (2026) Immunoinformatics-based design of a multiple-epitope vaccine against IHNV. Mar Biotechnol (NY) 28(3):79. https:\/\/doi.org\/10.1007\/s10126-026-10621-6","journal-title":"Mar Biotechnol (NY)"},{"key":"10707_CR2","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1016\/j.softx.2015.06.001","volume":"1\u20132","author":"MJ Abraham","year":"2015","unstructured":"Abraham MJ, Murtola T, Schulz R, P\u00e1ll S, Smith JC, Hess B, Lindahl E (2015) GROMACS: high performance molecular simulations through multi-level parallelism from laptops to supercomputers. SoftwareX 1\u20132:19\u201325. https:\/\/doi.org\/10.1016\/j.softx.2015.06.001","journal-title":"SoftwareX"},{"issue":"8016","key":"10707_CR3","doi-asserted-by":"publisher","first-page":"493","DOI":"10.1038\/s41586-024-07487-w","volume":"630","author":"J Abramson","year":"2024","unstructured":"Abramson J, Adler J, Dunger J et al (2024) Accurate structure prediction of biomolecular interactions with AlphaFold 3. Nature 630(8016):493\u2013500. https:\/\/doi.org\/10.1038\/s41586-024-07487-w","journal-title":"Nature"},{"issue":"7","key":"10707_CR4","doi-asserted-by":"publisher","first-page":"2963","DOI":"10.1080\/07391102.2020.1850357","volume":"40","author":"VS Ayyagari","year":"2022","unstructured":"Ayyagari VS, T C V, K AP, Srirama K (2022) Design of a multi-epitope-based vaccine targeting M-protein of SARS-CoV2: an immunoinformatics approach. J Biomol Struct Dyn 40(7):2963\u20132977. https:\/\/doi.org\/10.1080\/07391102.2020.1850357","journal-title":"J Biomol Struct Dyn"},{"issue":"11","key":"10707_CR5","doi-asserted-by":"publisher","first-page":"114107","DOI":"10.1063\/5.0020514","volume":"153","author":"M Bernetti","year":"2020","unstructured":"Bernetti M, Bussi G (2020) Pressure control using stochastic cell rescaling. J Chem Phys 153(11):114107. https:\/\/doi.org\/10.1063\/5.0020514","journal-title":"J Chem Phys"},{"issue":"W1","key":"10707_CR6","doi-asserted-by":"publisher","first-page":"W402","DOI":"10.1093\/nar\/gkz297","volume":"47","author":"DWA Buchan","year":"2019","unstructured":"Buchan DWA, Jones DT (2019) The PSIPRED Protein Analysis Workbench: 20 years on. Nucleic Acids Res 47(W1):W402\u2013W407. https:\/\/doi.org\/10.1093\/nar\/gkz297","journal-title":"Nucleic Acids Res"},{"issue":"1","key":"10707_CR7","doi-asserted-by":"publisher","first-page":"014101","DOI":"10.1063\/1.2408420","volume":"126","author":"G Bussi","year":"2007","unstructured":"Bussi G, Donadio D, Parrinello M (2007) Canonical sampling through velocity rescaling. J Chem Phys 126(1):014101. https:\/\/doi.org\/10.1063\/1.2408420","journal-title":"J Chem Phys"},{"issue":"10","key":"10707_CR8","doi-asserted-by":"publisher","first-page":"3668","DOI":"10.4049\/jimmunol.1600229","volume":"199","author":"Z Chen","year":"2017","unstructured":"Chen Z, Zhang N, Qi J, Chen R, Dijkstra JM, Li X, Wang Z, Wang J, Wu Y, Xia C (2017) The structure of the MHC class I molecule of bony fishes provides insights into the conserved nature of the antigen-presenting system. J Immunol 199(10):3668\u20133678. https:\/\/doi.org\/10.4049\/jimmunol.1600229","journal-title":"J Immunol"},{"key":"10707_CR9","doi-asserted-by":"publisher","first-page":"109502","DOI":"10.1016\/j.fsi.2024.109502","volume":"148","author":"H Chi","year":"2024","unstructured":"Chi H, Qin Q, Hao X, Dalmo RA, Tang X, Xing J, Sheng X, Zhan W (2024) Adjuvant effects of \u03b2-defensin on DNA vaccine OmpC against edwardsiellosis in flounder (Paralichthys olivaceus). Fish Shellfish Immunol 148:109502. https:\/\/doi.org\/10.1016\/j.fsi.2024.109502","journal-title":"Fish Shellfish Immunol"},{"issue":"12","key":"10707_CR10","doi-asserted-by":"publisher","first-page":"e4497","DOI":"10.1002\/pro.4497","volume":"31","author":"JN Clifford","year":"2022","unstructured":"Clifford JN, H\u00f8ie MH, Deleuran S, Peters B, Nielsen M, Marcatili P (2022) BepiPred-3.0: improved B-cell epitope prediction using protein language models. Protein Sci 31(12):e4497. https:\/\/doi.org\/10.1002\/pro.4497","journal-title":"Protein Sci"},{"issue":"9","key":"10707_CR11","doi-asserted-by":"publisher","first-page":"1511","DOI":"10.1002\/pro.5560020916","volume":"2","author":"C Colovos","year":"1993","unstructured":"Colovos C, Yeates TO (1993) Verification of protein structures: patterns of nonbonded atomic interactions. Protein Sci 2(9):1511\u20131519. https:\/\/doi.org\/10.1002\/pro.5560020916","journal-title":"Protein Sci"},{"issue":"6","key":"10707_CR12","doi-asserted-by":"publisher","first-page":"2278","DOI":"10.1007\/s00894-014-2278-5","volume":"20","author":"I Dimitrov","year":"2014","unstructured":"Dimitrov I, Bangov I, Flower DR, Doytchinova I (2014) AllerTOP v.2\u2014a server for in silico prediction of allergens. J Mol Model 20(6):2278. https:\/\/doi.org\/10.1007\/s00894-014-2278-5","journal-title":"J Mol Model"},{"issue":"6","key":"10707_CR13","doi-asserted-by":"publisher","first-page":"2869","DOI":"10.1128\/JVI.01444-10","volume":"85","author":"CF Dong","year":"2011","unstructured":"Dong CF, Xiong XP, Shuang F, Weng SP, Zhang J, Zhang Y, Luo YW, He JG (2011) Global landscape of structural proteins of infectious spleen and kidney necrosis virus. J Virol 85(6):2869\u20132877. https:\/\/doi.org\/10.1128\/JVI.01444-10","journal-title":"J Virol"},{"key":"10707_CR14","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1186\/1471-2105-8-4","volume":"8","author":"IA Doytchinova","year":"2007","unstructured":"Doytchinova IA, Flower DR (2007) VaxiJen: a server for prediction of protective antigens, tumour antigens and subunit vaccines. BMC Bioinform 8:4. https:\/\/doi.org\/10.1186\/1471-2105-8-4","journal-title":"BMC Bioinform"},{"key":"10707_CR15","doi-asserted-by":"publisher","first-page":"28","DOI":"10.3389\/fimmu.2013.00028","volume":"4","author":"S Fillatreau","year":"2013","unstructured":"Fillatreau S, Six A, Magadan S, Castro R, Sunyer JO, Boudinot P (2013) The astonishing diversity of Ig classes and B cell repertoires in teleost fish. Front Immunol 4:28. https:\/\/doi.org\/10.3389\/fimmu.2013.00028","journal-title":"Front Immunol"},{"issue":"3","key":"10707_CR16","doi-asserted-by":"publisher","first-page":"e0449522","DOI":"10.1128\/spectrum.04495-22","volume":"11","author":"W Fu","year":"2023","unstructured":"Fu W, Li Y, Fu Y, Zhang W, Luo P, Sun Q, Yu F, Weng S, Li W, He J, Dong C (2023) The inactivated ISKNV-I vaccine confers highly effective cross-protection against epidemic RSIV-I and RSIV-II from cultured spotted sea bass Lateolabrax maculatus. Microbiol Spectr 11(3):e0449522. https:\/\/doi.org\/10.1128\/spectrum.04495-22","journal-title":"Microbiol Spectr"},{"issue":"22","key":"10707_CR17","doi-asserted-by":"publisher","first-page":"3718","DOI":"10.1093\/bioinformatics\/btv428","volume":"31","author":"T Galili","year":"2015","unstructured":"Galili T (2015) dendextend: an R package for visualizing, adjusting and comparing trees of hierarchical clustering. Bioinformatics 31(22):3718\u20133720. https:\/\/doi.org\/10.1093\/bioinformatics\/btv428","journal-title":"Bioinformatics"},{"key":"10707_CR18","doi-asserted-by":"publisher","first-page":"136","DOI":"10.1016\/j.antiviral.2013.11.009","volume":"101","author":"P Garc\u00eda-Valtanen","year":"2014","unstructured":"Garc\u00eda-Valtanen P, Martinez-Lopez A, Ortega-Villaizan M, Perez L, Coll JM, Estepa A (2014) In addition to its antiviral and immunomodulatory properties, the zebrafish \u03b2-defensin 2 (zfBD2) is a potent viral DNA vaccine molecular adjuvant. Antiviral Res 101:136\u2013147. https:\/\/doi.org\/10.1016\/j.antiviral.2013.11.009","journal-title":"Antiviral Res"},{"key":"10707_CR19","doi-asserted-by":"crossref","unstructured":"Gasteiger E, Hoogland C, Gattiker A, Duvaud S, Wilkins MR, Appel RD, Bairoch A (2005) Protein identification and analysis tools on the Expasy server. In: Walker JM (ed) The proteomics protocols handbook. Humana, pp 571\u2013607","DOI":"10.1385\/1-59259-890-0:571"},{"issue":"4","key":"10707_CR20","doi-asserted-by":"publisher","first-page":"e0005023","DOI":"10.1128\/jvi.00050-23","volume":"97","author":"YM Gong","year":"2023","unstructured":"Gong YM, Wei XF, Zheng YY, Li Y, Yu Q, Li PF, Zhu B (2023) Combining phage display technology with in silico-designed epitope vaccine to elicit robust antibody responses against emerging pathogen tilapia lake virus. J Virol 97(4):e0005023. https:\/\/doi.org\/10.1128\/jvi.00050-23","journal-title":"J Virol"},{"issue":"19","key":"10707_CR22","doi-asserted-by":"publisher","first-page":"2811","DOI":"10.1093\/bioinformatics\/btu393","volume":"30","author":"Z Gu","year":"2014","unstructured":"Gu Z, Gu L, Eils R, Schlesner M, Brors B (2014) circlize implements and enhances circular visualization in R. Bioinformatics 30(19):2811\u20132812. https:\/\/doi.org\/10.1093\/bioinformatics\/btu393","journal-title":"Bioinformatics"},{"issue":"18","key":"10707_CR21","doi-asserted-by":"publisher","first-page":"2847","DOI":"10.1093\/bioinformatics\/btw313","volume":"32","author":"Z Gu","year":"2016","unstructured":"Gu Z, Eils R, Schlesner M (2016) Complex heatmaps reveal patterns and correlations in multidimensional genomic data. Bioinformatics 32(18):2847\u20132849. https:\/\/doi.org\/10.1093\/bioinformatics\/btw313","journal-title":"Bioinformatics"},{"issue":"3","key":"10707_CR23","doi-asserted-by":"publisher","first-page":"e70025","DOI":"10.1111\/raq.70025","volume":"17","author":"C Guo","year":"2025","unstructured":"Guo C, He J, Xu X, Weng S, He J (2025) Megalocytivirus: a review of epidemiology, pathogenicity, immune evasion, and prevention strategies. Rev Aquacult 17(3):e70025. https:\/\/doi.org\/10.1111\/raq.70025","journal-title":"Rev Aquacult"},{"key":"10707_CR24","doi-asserted-by":"crossref","unstructured":"Hallgren J, Tsirigos KD, Pedersen MD, Almagro Armenteros JJ, Marcatili P, Nielsen H, Krogh A, Winther O (2022) DeepTMHMM predicts alpha and beta transmembrane proteins using deep neural networks. bioRxiv, 2022.04.08.487609","DOI":"10.1101\/2022.04.08.487609"},{"issue":"19","key":"10707_CR25","doi-asserted-by":"publisher","first-page":"3098","DOI":"10.1093\/bioinformatics\/btx345","volume":"33","author":"M Hebditch","year":"2017","unstructured":"Hebditch M, Carballo-Amador MA, Charonis S, Curtis R, Warwicker J (2017) Protein-Sol: a web tool for predicting protein solubility from sequence. Bioinformatics 33(19):3098\u20133100. https:\/\/doi.org\/10.1093\/bioinformatics\/btx345","journal-title":"Bioinformatics"},{"issue":"11","key":"10707_CR26","doi-asserted-by":"publisher","first-page":"3219","DOI":"10.1038\/s41596-024-01011-0","volume":"19","author":"RV Honorato","year":"2024","unstructured":"Honorato RV, Trellet ME, Jim\u00e9nez-Garc\u00eda B, Schaarschmidt JJ, Giulini M, Reys V, Koukos PI, Rodrigues JPGLM, Karaca E, van Zundert GCP, Roel-Touris J, van Noort CW, Jandov\u00e1 Z, Melquiond ASJ, Bonvin AMJJ (2024) The HADDOCK2.4 web server for integrative modeling of biomolecular complexes. Nat Protoc 19(11):3219\u20133241. https:\/\/doi.org\/10.1038\/s41596-024-01011-0","journal-title":"Nat Protoc"},{"issue":"1","key":"10707_CR27","doi-asserted-by":"publisher","first-page":"5114","DOI":"10.1038\/s41467-018-07641-9","volume":"9","author":"C Jain","year":"2018","unstructured":"Jain C, Rodriguez-R LM, Phillippy AM, Konstantinidis KT, Aluru S (2018) High throughput ANI analysis of 90K prokaryotic genomes reveals clear species boundaries. Nat Commun 9(1):5114. https:\/\/doi.org\/10.1038\/s41467-018-07641-9","journal-title":"Nat Commun"},{"issue":"2","key":"10707_CR28","doi-asserted-by":"publisher","first-page":"926","DOI":"10.1063\/1.445869","volume":"79","author":"WL Jorgensen","year":"1983","unstructured":"Jorgensen WL, Chandrasekhar J, Madura JD, Impey RW, Klein ML (1983) Comparison of simple potential functions for simulating liquid water. J Chem Phys 79(2):926\u2013935. https:\/\/doi.org\/10.1063\/1.445869","journal-title":"J Chem Phys"},{"issue":"1","key":"10707_CR29","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1007\/s13721-021-00315-5","volume":"10","author":"A Joshi","year":"2021","unstructured":"Joshi A, Pathak DC, Mannan MAU, Kaushik V (2021) In-silico designing of epitope-based vaccine against the seven banded grouper nervous necrosis virus affecting fish species. Netw Model Anal Health Inf Bioinform 10(1):37. https:\/\/doi.org\/10.1007\/s13721-021-00315-5","journal-title":"Netw Model Anal Health Inf Bioinform"},{"issue":"8","key":"10707_CR30","doi-asserted-by":"publisher","first-page":"729","DOI":"10.1111\/jfd.12286","volume":"38","author":"MH Jung","year":"2015","unstructured":"Jung MH, Jung SJ, Vinay TN, Nikapitiya C, Kim JO, Lee JH, Lee J, Oh MJ (2015) Effects of water temperature on mortality in Megalocytivirus-infected rock bream Oplegnathus fasciatus (Temminck et Schlegel) and development of protective immunity. J Fish Dis 38(8):729\u2013737. https:\/\/doi.org\/10.1111\/jfd.12286","journal-title":"J Fish Dis"},{"issue":"6","key":"10707_CR31","doi-asserted-by":"publisher","first-page":"802","DOI":"10.1016\/j.vaccine.2017.12.077","volume":"36","author":"MH Jung","year":"2018","unstructured":"Jung MH, Nikapitiya C, Jung SJ (2018) DNA vaccine encoding myristoylated membrane protein (MMP) of rock bream iridovirus (RBIV) induces protective immunity in rock bream (Oplegnathus fasciatus). Vaccine 36(6):802\u2013810. https:\/\/doi.org\/10.1016\/j.vaccine.2017.12.077","journal-title":"Vaccine"},{"issue":"7","key":"10707_CR32","doi-asserted-by":"publisher","first-page":"1210","DOI":"10.3390\/ani13071210","volume":"13","author":"KH Kim","year":"2023","unstructured":"Kim KH, Kang G, Woo WS, Sohn MY, Son HJ, Kwon MG, Kim JO, Park CI (2023) Impact of red sea bream iridovirus infection on rock bream (Oplegnathus fasciatus) and other fish species: a study of horizontal transmission. Anim (Basel) 13(7):1210. https:\/\/doi.org\/10.3390\/ani13071210","journal-title":"Anim (Basel)"},{"issue":"4","key":"10707_CR33","doi-asserted-by":"publisher","first-page":"521","DOI":"10.3390\/v4040521","volume":"4","author":"J Kurita","year":"2012","unstructured":"Kurita J, Nakajima K (2012) Megalocytiviruses Viruses 4(4):521\u2013538. https:\/\/doi.org\/10.3390\/v4040521","journal-title":"Megalocytiviruses Viruses"},{"issue":"10","key":"10707_CR36","doi-asserted-by":"publisher","first-page":"2778","DOI":"10.1021\/ci200227u","volume":"51","author":"RA Laskowski","year":"2011","unstructured":"Laskowski RA, Swindells MB (2011) LigPlot+: multiple ligand-protein interaction diagrams for drug discovery. J Chem Inf Model 51(10):2778\u20132786. https:\/\/doi.org\/10.1021\/ci200227u","journal-title":"J Chem Inf Model"},{"issue":"2","key":"10707_CR35","doi-asserted-by":"publisher","first-page":"283","DOI":"10.1107\/S0021889892009944","volume":"26","author":"RA Laskowski","year":"1993","unstructured":"Laskowski RA, MacArthur MW, Moss DS, Thornton JM (1993) PROCHECK: a program to check the stereochemical quality of protein structures. J Appl Crystallogr 26(2):283\u2013291. https:\/\/doi.org\/10.1107\/S0021889892009944","journal-title":"J Appl Crystallogr"},{"issue":"1","key":"10707_CR34","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1002\/pro.3289","volume":"27","author":"RA Laskowski","year":"2018","unstructured":"Laskowski RA, Jab\u0142o\u0144ska J, Pravda L, Va\u0159ekov\u00e1 RS, Thornton JM (2018) PDBsum: structural summaries of PDB entries. Protein Sci 27(1):129\u2013134. https:\/\/doi.org\/10.1002\/pro.3289","journal-title":"Protein Sci"},{"issue":"W1","key":"10707_CR37","doi-asserted-by":"publisher","first-page":"W451","DOI":"10.1093\/nar\/gkz288","volume":"47","author":"GR Lee","year":"2019","unstructured":"Lee GR, Won J, Heo L, Seok C (2019) GalaxyRefine2: simultaneous refinement of inaccurate local regions and overall protein structure. Nucleic Acids Res 47(W1):W451\u2013W455. https:\/\/doi.org\/10.1093\/nar\/gkz288","journal-title":"Nucleic Acids Res"},{"issue":"1","key":"10707_CR38","doi-asserted-by":"publisher","first-page":"88","DOI":"10.1186\/s13567-024-01347-1","volume":"55","author":"R Leiva-Rebollo","year":"2024","unstructured":"Leiva-Rebollo R, Labella AM, G\u00e9mez-Mata J, Castro D, Borrego JJ (2024) Fish Iridoviridae: infection, vaccination and immune response. Vet Res 55(1):88. https:\/\/doi.org\/10.1186\/s13567-024-01347-1","journal-title":"Vet Res"},{"issue":"2","key":"10707_CR39","doi-asserted-by":"publisher","first-page":"137","DOI":"10.3390\/vaccines12020137","volume":"12","author":"S Liang","year":"2024","unstructured":"Liang S, Zhang S, Bao Y, Zhang Y, Liu X, Yao H, Liu G (2024) Combined immunoinformatics to design and evaluate a multi-epitope vaccine candidate against Streptococcus suis infection. Vaccines 12(2):137. https:\/\/doi.org\/10.3390\/vaccines12020137","journal-title":"Vaccines"},{"issue":"8","key":"10707_CR40","doi-asserted-by":"publisher","first-page":"1950","DOI":"10.1002\/prot.22711","volume":"78","author":"K Lindorff-Larsen","year":"2010","unstructured":"Lindorff-Larsen K, Piana S, Palmo K, Maragakis P, Klepeis JL, Dror RO, Shaw DE (2010) Improved side-chain torsion potentials for the Amber ff99SB protein force field. Proteins 78(8):1950\u20131958. https:\/\/doi.org\/10.1002\/prot.22711","journal-title":"Proteins"},{"key":"10707_CR41","doi-asserted-by":"publisher","first-page":"1528930","DOI":"10.3389\/fmicb.2025.1528930","volume":"16","author":"X Liu","year":"2025","unstructured":"Liu X, Chi H, Yang X, Zheng Z, Zhu C, Wu Y, Chang WJ, Gong H (2025) A comprehensive analysis of the genomic and proteomic profiles of a megalocytivirus isolated from Larimichthys crocea. Front Microbiol 16:1528930. https:\/\/doi.org\/10.3389\/fmicb.2025.1528930","journal-title":"Front Microbiol"},{"key":"10707_CR42","doi-asserted-by":"publisher","unstructured":"L\u00f3pez-Blanco JR, Aliaga JI, Quintana-Ort\u00ed ES, Chac\u00f3n P (2014) iMODS: internal coordinates normal mode analysis server. Nucleic Acids Res W271\u2013W276 42(Web Server issue). https:\/\/doi.org\/10.1093\/nar\/gku339","DOI":"10.1093\/nar\/gku339"},{"issue":"4","key":"10707_CR43","doi-asserted-by":"publisher","first-page":"45","DOI":"10.3390\/biology5040045","volume":"5","author":"S Mashoof","year":"2016","unstructured":"Mashoof S, Criscitiello MF (2016) Fish immunoglobulins. Biology 5(4):45. https:\/\/doi.org\/10.3390\/biology5040045","journal-title":"Biology"},{"issue":"6","key":"10707_CR44","doi-asserted-by":"publisher","first-page":"680","DOI":"10.3390\/vaccines12060680","volume":"12","author":"JG Min","year":"2024","unstructured":"Min JG, Kim GH, Kim CH, Kwon WJ, Jeong HD, Kim KI (2024) Evaluation of formalin-inactivated vaccine efficacy against red seabream Iridovirus (RSIV) in laboratory and field conditions. Vaccines 12(6):680. https:\/\/doi.org\/10.3390\/vaccines12060680","journal-title":"Vaccines"},{"issue":"4","key":"10707_CR45","doi-asserted-by":"publisher","first-page":"205","DOI":"10.3147\/jsfp.32.205","volume":"32","author":"K Nakajima","year":"1997","unstructured":"Nakajima K, Maeno Y, Kurita J, Inui Y (1997) Vaccination against red sea bream iridoviral disease in red sea bream. Fish Pathol 32(4):205\u2013209. https:\/\/doi.org\/10.3147\/jsfp.32.205","journal-title":"Fish Pathol"},{"issue":"47","key":"10707_CR47","doi-asserted-by":"publisher","first-page":"eadj6367","DOI":"10.1126\/sciadv.adj6367","volume":"9","author":"JB Nilsson","year":"2023","unstructured":"Nilsson JB, Kaabinejadian S, Yari H, Kester MGD, van Balen P, Hildebrand WH, Nielsen M (2023) Accurate prediction of HLA class II antigen presentation across all loci using tailored data acquisition and refined machine learning. Sci Adv 9(47):eadj6367. https:\/\/doi.org\/10.1126\/sciadv.adj6367","journal-title":"Sci Adv"},{"key":"10707_CR46","doi-asserted-by":"publisher","first-page":"1616113","DOI":"10.3389\/fimmu.2025.1616113","volume":"16","author":"JB Nilsson","year":"2025","unstructured":"Nilsson JB, Greenbaum J, Peters B, Nielsen M (2025) NetMHCpan-4.2: improved prediction of CD8\u2009+\u2009epitopes by use of transfer learning and structural features. Front Immunol 16:1616113. https:\/\/doi.org\/10.3389\/fimmu.2025.1616113","journal-title":"Front Immunol"},{"issue":"22","key":"10707_CR48","doi-asserted-by":"publisher","first-page":"3691","DOI":"10.1093\/bioinformatics\/btv421","volume":"31","author":"AJ Page","year":"2015","unstructured":"Page AJ, Cummins CA, Hunt M, Wong VK, Reuter S, Holden MTG, Fookes M, Falush D, Keane JA, Parkhill J (2015) Roary: rapid large-scale prokaryote pan genome analysis. Bioinformatics 31(22):3691\u20133693. https:\/\/doi.org\/10.1093\/bioinformatics\/btv421","journal-title":"Bioinformatics"},{"issue":"12","key":"10707_CR49","doi-asserted-by":"publisher","first-page":"1263","DOI":"10.1016\/j.dci.2011.03.006","volume":"35","author":"Y Palti","year":"2011","unstructured":"Palti Y (2011) Toll-like receptors in bony fish: from genomics to function. Dev Comp Immunol 35(12):1263\u20131272. https:\/\/doi.org\/10.1016\/j.dci.2011.03.006","journal-title":"Dev Comp Immunol"},{"key":"10707_CR50","doi-asserted-by":"publisher","first-page":"514","DOI":"10.1186\/1471-2105-9-514","volume":"9","author":"JV Ponomarenko","year":"2008","unstructured":"Ponomarenko JV, Bui HH, Li W, Fusseder N, Bourne PE, Sette A, Peters B (2008) ElliPro: a new structure-based tool for the prediction of antibody epitopes. BMC Bioinform 9:514. https:\/\/doi.org\/10.1186\/1471-2105-9-514","journal-title":"BMC Bioinform"},{"issue":"3","key":"10707_CR51","doi-asserted-by":"publisher","first-page":"460","DOI":"10.3390\/biology4030460","volume":"4","author":"S Poynter","year":"2015","unstructured":"Poynter S, Lisser G, Monjo A, DeWitte-Orr S (2015) Sensors of infection: viral nucleic acid PRRs in fish. Biology 4(3):460\u2013493. https:\/\/doi.org\/10.3390\/biology4030460","journal-title":"Biology"},{"issue":"1","key":"10707_CR52","doi-asserted-by":"publisher","first-page":"14048","DOI":"10.1038\/s41598-024-64383-z","volume":"14","author":"A Pumchan","year":"2024","unstructured":"Pumchan A, Proespraiwong P, Sawatdichaikul O, Phurahong T, Hirono I, Unajak S (2024) Computational design of novel chimeric multiepitope vaccine against bacterial and viral disease in tilapia (Oreochromis sp). Sci Rep 14(1):14048. https:\/\/doi.org\/10.1038\/s41598-024-64383-z","journal-title":"Sci Rep"},{"issue":"4","key":"10707_CR53","doi-asserted-by":"publisher","first-page":"4007","DOI":"10.1021\/acsomega.4c09890","volume":"10","author":"NT Randriamamisolonirina","year":"2025","unstructured":"Randriamamisolonirina NT, Razafindrafara MS, Maminiaina OF (2025) Design of a multi-epitope vaccine against the glycoproteins of Newcastle disease virus by using an immunoinformatics approach. ACS Omega 10(4):4007\u20134018. https:\/\/doi.org\/10.1021\/acsomega.4c09890","journal-title":"ACS Omega"},{"issue":"1","key":"10707_CR54","doi-asserted-by":"publisher","first-page":"11783","DOI":"10.1038\/s41598-024-61074-7","volume":"14","author":"NA Rani","year":"2024","unstructured":"Rani NA, Robin TB, Prome AA, Ahmed N, Moin AT, Patil RB, Sikder MNA, Bappy MNI, Afrin D, Hossain FMA, Islam T, Zinnah KMA (2024) Development of multi epitope subunit vaccines against emerging carp viruses Cyprinid herpesvirus 1 and 3 using immunoinformatics approach. Sci Rep 14(1):11783. https:\/\/doi.org\/10.1038\/s41598-024-61074-7","journal-title":"Sci Rep"},{"issue":"4","key":"10707_CR55","doi-asserted-by":"publisher","first-page":"e9862","DOI":"10.1371\/journal.pone.0009862","volume":"5","author":"N Rapin","year":"2010","unstructured":"Rapin N, Lund O, Bernaschi M, Castiglione F (2010) Computational immunology meets bioinformatics: the use of prediction tools for molecular binding in the simulation of the immune system. PLoS ONE 5(4):e9862. https:\/\/doi.org\/10.1371\/journal.pone.0009862","journal-title":"PLoS ONE"},{"issue":"14","key":"10707_CR56","doi-asserted-by":"publisher","first-page":"2013","DOI":"10.1093\/bioinformatics\/btr335","volume":"27","author":"N Rapin","year":"2011","unstructured":"Rapin N, Lund O, Castiglione F (2011) Immune system simulation online. Bioinformatics 27(14):2013\u20132014. https:\/\/doi.org\/10.1093\/bioinformatics\/btr335","journal-title":"Bioinformatics"},{"key":"10707_CR57","doi-asserted-by":"publisher","first-page":"108926","DOI":"10.1016\/j.compbiomed.2024.108926","volume":"179","author":"AS Rathore","year":"2024","unstructured":"Rathore AS, Choudhury S, Arora A, Tijare P, Raghava GPS (2024) ToxinPred 3.0: an improved method for predicting the toxicity of peptides. Comput Biol Med 179:108926. https:\/\/doi.org\/10.1016\/j.compbiomed.2024.108926","journal-title":"Comput Biol Med"},{"issue":"W1","key":"10707_CR58","doi-asserted-by":"publisher","first-page":"W432","DOI":"10.1093\/nar\/gkad376","volume":"51","author":"J Rey","year":"2023","unstructured":"Rey J, Murail S, de Vries S, Derreumaux P, Tuffery P (2023) PEP-FOLD4: a pH-dependent force field for peptide structure prediction in aqueous solution. Nucleic Acids Res 51(W1):W432\u2013W437. https:\/\/doi.org\/10.1093\/nar\/gkad376","journal-title":"Nucleic Acids Res"},{"issue":"11","key":"10707_CR59","doi-asserted-by":"publisher","first-page":"222","DOI":"10.1007\/s00705-025-06430-2","volume":"170","author":"NG Ryu","year":"2025","unstructured":"Ryu NG, Yang YJ, Kim KI (2025) Application of a multi-epitope DNA vaccine against viral hemorrhagic septicemia virus type IVa. Arch Virol 170(11):222. https:\/\/doi.org\/10.1007\/s00705-025-06430-2","journal-title":"Arch Virol"},{"key":"10707_CR60","doi-asserted-by":"publisher","first-page":"104079","DOI":"10.1016\/j.dci.2021.104079","volume":"121","author":"I Salinas","year":"2021","unstructured":"Salinas I, Fern\u00e1ndez-Montero \u00c1, Ding Y, Sunyer JO (2021) Mucosal immunoglobulins of teleost fish: a decade of advances. Dev Comp Immunol 121:104079. https:\/\/doi.org\/10.1016\/j.dci.2021.104079","journal-title":"Dev Comp Immunol"},{"issue":"14","key":"10707_CR61","doi-asserted-by":"publisher","first-page":"2068","DOI":"10.1093\/bioinformatics\/btu153","volume":"30","author":"T Seemann","year":"2014","unstructured":"Seemann T (2014) Prokka: rapid prokaryotic genome annotation. Bioinformatics 30(14):2068\u20132069. https:\/\/doi.org\/10.1093\/bioinformatics\/btu153","journal-title":"Bioinformatics"},{"issue":"45","key":"10707_CR62","doi-asserted-by":"publisher","first-page":"5210","DOI":"10.1016\/j.vaccine.2013.08.085","volume":"31","author":"YJ Shin","year":"2013","unstructured":"Shin YJ, Kwon TH, Seo JY, Kim TJ (2013) Oral immunization of fish against iridovirus infection using recombinant antigen produced from rice callus. Vaccine 31(45):5210\u20135215. https:\/\/doi.org\/10.1016\/j.vaccine.2013.08.085","journal-title":"Vaccine"},{"issue":"1","key":"10707_CR63","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1016\/j.dci.2013.12.008","volume":"44","author":"T Somamoto","year":"2014","unstructured":"Somamoto T, Kondo M, Nakanishi T, Nakao M (2014) Helper function of CD4\u207a lymphocytes in antiviral immunity in ginbuna crucian carp, Carassius auratus langsdorfii. Dev Comp Immunol 44(1):111\u2013115. https:\/\/doi.org\/10.1016\/j.dci.2013.12.008","journal-title":"Dev Comp Immunol"},{"issue":"4","key":"10707_CR64","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1111\/j.1753-5131.2012.01075.x","volume":"4","author":"K Subramaniam","year":"2012","unstructured":"Subramaniam K, Shariff M, Omar AR, Hair-Bejo M (2012) Megalocytivirus infection in fish. Rev Aquacult 4(4):221\u2013233. https:\/\/doi.org\/10.1111\/j.1753-5131.2012.01075.x","journal-title":"Rev Aquacult"},{"issue":"7","key":"10707_CR65","doi-asserted-by":"publisher","first-page":"1023","DOI":"10.1038\/s41587-021-01156-3","volume":"40","author":"F Teufel","year":"2022","unstructured":"Teufel F, Almagro Armenteros JJ, Johansen AR, G\u00edslason MH, Pihl SI, Tsirigos KD, Winther O, Brunak S, von Heijne G, Nielsen H (2022) SignalP 6.0 predicts all five types of signal peptides using protein language models. Nat Biotechnol 40(7):1023\u20131025. https:\/\/doi.org\/10.1038\/s41587-021-01156-3","journal-title":"Nat Biotechnol"},{"key":"10707_CR75","doi-asserted-by":"publisher","unstructured":"Weber F, Wagner V, Rasmussen SB, Hartmann R, Paludan SR (2006) Double-stranded RNA is produced by positive-strand RNA viruses and DNA viruses but not indetectable amounts by negative-strand RNA viruses. J Virol 80(10):5059\u20135064. https:\/\/doi.org\/10.1128\/JVI.80.10.5059-5064.2006","DOI":"10.1128\/JVI.80.10.5059-5064.2006"},{"issue":"12","key":"10707_CR66","doi-asserted-by":"publisher","first-page":"1","DOI":"10.18637\/jss.v021.i12","volume":"21","author":"H Wickham","year":"2007","unstructured":"Wickham H (2007) Reshaping data with the reshape package. J Stat Softw 21(12):1\u201320","journal-title":"J Stat Softw"},{"key":"10707_CR67","doi-asserted-by":"publisher","unstructured":"Wiederstein M, Sippl MJ (2007) ProSA-web: interactive web service for the recognition of errors in three-dimensional structures of proteins. Nucleic Acids Res 35(Web Server issue)(Web server issue):W407\u2013W410. https:\/\/doi.org\/10.1093\/nar\/gkm290","DOI":"10.1093\/nar\/gkm290"},{"key":"10707_CR68","unstructured":"World Organisation for Animal Health (WOAH) (2025) Red sea bream iridoviral disease. In: Manual of Diagnostic Tests for Aquatic Animals, Chapter 2.3.7. World Organisation for Animal Health, Paris, France, pp 1\u201312. https:\/\/www.woah.org\/fileadmin\/Home\/eng\/Health_standards\/aahm\/current\/2.3.07_RSIVD.pdf"},{"key":"10707_CR69","unstructured":"World Organisation for Animal Health (WOAH) (2026) Infection with Megalocytivirus pagrus1. Technical Disease Card. World Organisation for Animal Health, Paris, France, pp 1\u20135. https:\/\/www.woah.org\/app\/uploads\/2025\/03\/dis-card-mpagrus1-march26.pdf"},{"issue":"6","key":"10707_CR70","doi-asserted-by":"publisher","first-page":"919","DOI":"10.1016\/j.fsi.2011.08.009","volume":"31","author":"XP Xiong","year":"2011","unstructured":"Xiong XP, Dong CF, Weng SP, Zhang J, Zhang Y, He JG (2011) Antigenic identification of virion structural proteins from infectious spleen and kidney necrosis virus. Fish Shellfish Immunol 31(6):919\u2013924. https:\/\/doi.org\/10.1016\/j.fsi.2011.08.009","journal-title":"Fish Shellfish Immunol"},{"issue":"1","key":"10707_CR71","doi-asserted-by":"publisher","first-page":"389","DOI":"10.1186\/s12964-025-02411-9","volume":"23","author":"FF Xu","year":"2025","unstructured":"Xu FF, Zhao Z, Deng ZY, Tang JL, Zhu B (2025) Self-assembled nanoparticle vaccine comprised of multiple epitopes provides robust protective immunity against reoviruses in fish model. Cell Commun Signal 23(1):389. https:\/\/doi.org\/10.1186\/s12964-025-02411-9","journal-title":"Cell Commun Signal"},{"issue":"5","key":"10707_CR72","doi-asserted-by":"publisher","first-page":"1829","DOI":"10.1038\/s41596-020-0312-x","volume":"15","author":"Y Yan","year":"2020","unstructured":"Yan Y, Tao H, He J, Huang SY (2020) The HDOCK server for integrated protein\u2013protein docking. Nat Protoc 15(5):1829\u20131852. https:\/\/doi.org\/10.1038\/s41596-020-0312-x","journal-title":"Nat Protoc"},{"key":"10707_CR74","doi-asserted-by":"publisher","unstructured":"Zhang SY, Jouanguy E, Ugolini S et al (2007) TLR3 deficiency in patients with herpes simplex encephalitis. Science 317(5844):1522\u20131527. https:\/\/doi.org\/10.1126\/science.1139522","DOI":"10.1126\/science.1139522"},{"issue":"11","key":"10707_CR73","doi-asserted-by":"publisher","first-page":"e112918","DOI":"10.1371\/journal.pone.0112918","volume":"9","author":"ZX Zhou","year":"2014","unstructured":"Zhou ZX, Zhang BC, Sun L (2014) Poly(I:C) induces antiviral immune responses in Japanese flounder (Paralichthys olivaceus) that require TLR3 and MDA5 and is negatively regulated by Myd88. PLoS ONE 9(11):e112918. https:\/\/doi.org\/10.1371\/journal.pone.0112918","journal-title":"PLoS ONE"}],"container-title":["Marine Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10126-026-10707-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10126-026-10707-1","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10126-026-10707-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T18:02:54Z","timestamp":1793296974000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10126-026-10707-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,17]]},"references-count":75,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10707"],"URL":"https:\/\/doi.org\/10.1007\/s10126-026-10707-1","relation":{},"ISSN":["1436-2228","1436-2236"],"issn-type":[{"value":"1436-2228","type":"print"},{"value":"1436-2236","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,17]]},"assertion":[{"value":"27 July 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"11 September 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"17 September 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"Not applicable.","order":1,"name":"Ethics","label":"Research involving human participants and\/or animals","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Not applicable.","order":2,"name":"Ethics","label":"Informed consent","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","label":"Competing interests","group":{"name":"EthicsHeading","label":"Declarations"}}],"article-number":"158"}}