{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T18:35:56Z","timestamp":1793298956227,"version":"4.5.0"},"reference-count":135,"publisher":"Springer Science and Business Media LLC","issue":"5","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Mar Biotechnol"],"published-print":{"date-parts":[[2026,10]]},"DOI":"10.1007\/s10126-026-10718-y","type":"journal-article","created":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T20:13:11Z","timestamp":1792008791000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Marine-Derived Extracellular Vesicles: Bioinspired Nanobiomaterials for Skin Regeneration and Dermatological Applications"],"prefix":"10.1007","volume":"28","author":[{"given":"Afsona","family":"Khatun","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rahul","family":"Samanta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ananda Mohan","family":"Dutta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Imtiaz","family":"Kabir","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sajal Kumar","family":"Jha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thirumal","family":"M","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,10,14]]},"reference":[{"key":"10718_CR1","doi-asserted-by":"publisher","first-page":"e202400486","DOI":"10.1002\/cbdv.202400486","volume":"21","author":"AA Abd-Rabou","year":"2024","unstructured":"Abd-Rabou AA, Kishta MS, Yakout SM et al (2024) Copper\/Tin Nanocomposites-Loaded Exosomes Induce Apoptosis and Cell Cycle Arrest at G0\/G1 Phase in Skin Cancer Cell Line. Chem Biodivers 21:e202400486. https:\/\/doi.org\/10.1002\/cbdv.202400486","journal-title":"Chem Biodivers"},{"key":"10718_CR2","doi-asserted-by":"publisher","first-page":"e12081","DOI":"10.1002\/jev2.12081","volume":"10","author":"G Adamo","year":"2021","unstructured":"Adamo G, Fierli D, Romancino DP et al (2021) Nanoalgosomes: Introducing extracellular vesicles produced by microalgae. J Extracell Vesicles 10:e12081. https:\/\/doi.org\/10.1002\/jev2.12081","journal-title":"J Extracell Vesicles"},{"key":"10718_CR3","unstructured":"Adamo G, Santonicola P, Picciotto S et al (2023) Microalgae as a novel biofactory for biocompatible and bioactive extracellular vesicles. 2023.04.04.535547"},{"key":"10718_CR4","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1111\/jth.12767","volume":"13","author":"N Arraud","year":"2015","unstructured":"Arraud N, Gounou C, Linares R, Brisson AR (2015) A simple flow cytometry method improves the detection of phosphatidylserine-exposing extracellular vesicles. J Thromb Haemost 13:237\u2013247. https:\/\/doi.org\/10.1111\/jth.12767","journal-title":"J Thromb Haemost"},{"key":"10718_CR5","doi-asserted-by":"publisher","first-page":"785716","DOI":"10.3389\/fmicb.2021.785716","volume":"12","author":"F Bayat","year":"2021","unstructured":"Bayat F, Afshar A, Baghban N (2021) Algal Cells-Derived Extracellular Vesicles: A Review With Special Emphasis on Their Antimicrobial Effects. Front Microbiol 12:785716. https:\/\/doi.org\/10.3389\/fmicb.2021.785716","journal-title":"Front Microbiol"},{"key":"10718_CR6","doi-asserted-by":"publisher","first-page":"e100","DOI":"10.1002\/jex2.100","volume":"2","author":"B Benayas","year":"2023","unstructured":"Benayas B, Morales J, Egea C et al (2023) Optimization of extracellular vesicle isolation and their separation from lipoproteins by size exclusion chromatography. J Extracell Biol 2:e100. https:\/\/doi.org\/10.1002\/jex2.100","journal-title":"J Extracell Biol"},{"key":"10718_CR7","doi-asserted-by":"publisher","unstructured":"Benito-Mart\u00edn A, Jasiulionis MG, Garc\u00eda-Silva S (2023) Extracellular vesicles and melanoma: new perspectives on tumor microenvironment and metastasis. Front Cell Dev Biol 10. https:\/\/doi.org\/10.3389\/fcell.2022.1061982","DOI":"10.3389\/fcell.2022.1061982"},{"key":"10718_CR8","doi-asserted-by":"publisher","first-page":"455","DOI":"10.1016\/j.urolonc.2021.03.010","volume":"39","author":"DE Bernstein","year":"2021","unstructured":"Bernstein DE, Piedad J, Hemsworth L et al (2021) Prostate cancer and microfluids. Urol Oncol Semin Orig Investig 39:455\u2013470. https:\/\/doi.org\/10.1016\/j.urolonc.2021.03.010","journal-title":"Urol Oncol Semin Orig Investig"},{"key":"10718_CR9","doi-asserted-by":"crossref","unstructured":"Bongiovanni A, Adamo G, Santonicola P et al (2023) Microalgae as a novel biofactory for biocompatible and bioactive extracellular vesicles","DOI":"10.21203\/rs.3.rs-2841234\/v1"},{"key":"10718_CR10","doi-asserted-by":"publisher","first-page":"120","DOI":"10.1016\/j.jim.2014.04.003","volume":"407","author":"Z Brownlee","year":"2014","unstructured":"Brownlee Z, Lynn KD, Thorpe PE, Schroit AJ (2014) A novel salting-out procedure for the isolation of tumor-derived exosomes. J Immunol Methods 407:120\u2013126. https:\/\/doi.org\/10.1016\/j.jim.2014.04.003","journal-title":"J Immunol Methods"},{"key":"10718_CR11","doi-asserted-by":"publisher","first-page":"273","DOI":"10.3390\/cells7120273","volume":"7","author":"S Busatto","year":"2018","unstructured":"Busatto S, Vilanilam G, Ticer T et al (2018a) Tangential Flow Filtration for Highly Efficient Concentration of Extracellular Vesicles from Large Volumes of Fluid. Cells 7:273. https:\/\/doi.org\/10.3390\/cells7120273","journal-title":"Cells"},{"key":"10718_CR12","doi-asserted-by":"publisher","first-page":"273","DOI":"10.3390\/cells7120273","volume":"7","author":"S Busatto","year":"2018","unstructured":"Busatto S, Vilanilam G, Ticer T et al (2018b) Tangential Flow Filtration for Highly Efficient Concentration of Extracellular Vesicles from Large Volumes of Fluid. Cells 7:273. https:\/\/doi.org\/10.3390\/cells7120273","journal-title":"Cells"},{"key":"10718_CR13","doi-asserted-by":"publisher","first-page":"107","DOI":"10.1016\/j.jsb.2009.03.007","volume":"168","author":"N Buzhynskyy","year":"2009","unstructured":"Buzhynskyy N, Golczak M, Lai-Kee-Him J et al (2009) Annexin-A6 presents two modes of association with phospholipid membranes. A combined QCM-D, AFM and cryo-TEM study. J Struct Biol 168:107\u2013116. https:\/\/doi.org\/10.1016\/j.jsb.2009.03.007","journal-title":"J Struct Biol"},{"key":"10718_CR14","doi-asserted-by":"publisher","first-page":"363","DOI":"10.3390\/md22080363","volume":"22","author":"A Casillo","year":"2024","unstructured":"Casillo A, D\u2019Amico R, Lanzetta R, Corsaro MM (2024) Marine Delivery Vehicles: Molecular Components and Applications of Bacterial Extracellular Vesicles. Mar Drugs 22:363. https:\/\/doi.org\/10.3390\/md22080363","journal-title":"Mar Drugs"},{"key":"10718_CR15","doi-asserted-by":"publisher","first-page":"1216","DOI":"10.1111\/j.1538-7836.2011.04283.x","volume":"9","author":"WL Chandler","year":"2011","unstructured":"Chandler WL, Yeung W, Tait JF (2011) A new microparticle size calibration standard for use in measuring smaller microparticles using a new flow cytometer. J Thromb Haemost 9:1216\u20131224. https:\/\/doi.org\/10.1111\/j.1538-7836.2011.04283.x","journal-title":"J Thromb Haemost"},{"key":"10718_CR16","doi-asserted-by":"publisher","first-page":"513","DOI":"10.1007\/s13770-024-00634-4","volume":"21","author":"S Che Shaffi","year":"2024","unstructured":"Che Shaffi S, Hairuddin ON, Mansor SF et al (2024) Unlocking the Potential of Extracellular Vesicles as the Next Generation Therapy: Challenges and Opportunities. Tissue Eng Regen Med 21:513\u2013527. https:\/\/doi.org\/10.1007\/s13770-024-00634-4","journal-title":"Tissue Eng Regen Med"},{"key":"10718_CR17","doi-asserted-by":"publisher","first-page":"e95240","DOI":"10.1371\/journal.pone.0095240","volume":"9","author":"W Chen","year":"2014","unstructured":"Chen W, Liu X, Lv M et al (2014) Exosomes from Drug-Resistant Breast Cancer Cells Transmit Chemoresistance by a Horizontal Transfer of MicroRNAs. PLoS ONE 9:e95240. https:\/\/doi.org\/10.1371\/journal.pone.0095240","journal-title":"PLoS ONE"},{"key":"10718_CR18","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1007\/978-1-4939-7253-1_18","volume-title":"Extracellular Vesicles: Methods and Protocols","author":"P Cizmar","year":"2017","unstructured":"Cizmar P, Yuana Y (2017) Detection and Characterization of Extracellular Vesicles by Transmission and Cryo-Transmission Electron Microscopy. In: Kuo WP, Jia S (eds) Extracellular Vesicles: Methods and Protocols. Springer, New York, NY, pp 221\u2013232"},{"key":"10718_CR19","doi-asserted-by":"publisher","unstructured":"Comfort N, Cai K, Bloomquist TR et al (2021) Nanoparticle tracking analysis for the quantification and size determination of extracellular vesicles. J Vis Exp JoVE e 62447. https:\/\/doi.org\/10.3791\/62447","DOI":"10.3791\/62447"},{"key":"10718_CR20","doi-asserted-by":"publisher","first-page":"114108","DOI":"10.1016\/j.addr.2021.114108","volume":"182","author":"M Cong","year":"2022","unstructured":"Cong M, Tan S, Li S et al (2022) Technology insight: Plant-derived vesicles\u2014How far from the clinical biotherapeutics and therapeutic drug carriers? Adv Drug Deliv Rev 182:114108. https:\/\/doi.org\/10.1016\/j.addr.2021.114108","journal-title":"Adv Drug Deliv Rev"},{"key":"10718_CR21","doi-asserted-by":"publisher","first-page":"866","DOI":"10.3390\/biology10090866","volume":"10","author":"S D\u2019Alessio","year":"2021","unstructured":"D\u2019Alessio S, Buckley KM, Kraev I et al (2021) Extracellular Vesicle Signatures and Post-Translational Protein Deimination in Purple Sea Urchin (Strongylocentrotus purpuratus) Coelomic Fluid\u2014Novel Insights into Echinodermata Biology. Biology 10:866. https:\/\/doi.org\/10.3390\/biology10090866","journal-title":"Biology"},{"key":"10718_CR22","doi-asserted-by":"publisher","first-page":"870","DOI":"10.3390\/biology14070870","volume":"14","author":"S D\u2019Alessio","year":"2025","unstructured":"D\u2019Alessio S, Kraev I, Magnad\u00f3ttir B, Lange S (2025) Exploring the Potential of Extracellular Vesicles from Atlantic Cod (Gadus morhua L.) Serum and Mucus for Wound Healing In Vitro. Biology 14:870. https:\/\/doi.org\/10.3390\/biology14070870","journal-title":"Biology"},{"key":"10718_CR23","doi-asserted-by":"publisher","first-page":"1925","DOI":"10.3390\/md13041925","volume":"13","author":"GZL Dalmaso","year":"2015","unstructured":"Dalmaso GZL, Ferreira D, Vermelho AB (2015) Marine Extremophiles: A Source of Hydrolases for Biotechnological Applications. Mar Drugs 13:1925\u20131965. https:\/\/doi.org\/10.3390\/md13041925","journal-title":"Mar Drugs"},{"key":"10718_CR24","doi-asserted-by":"publisher","first-page":"13247","DOI":"10.3390\/ijms241713247","volume":"24","author":"AA Danilushkina","year":"2023","unstructured":"Danilushkina AA, Emene CC, Barlev NA, Gomzikova MO (2023) Strategies for Engineering of Extracellular Vesicles. Int J Mol Sci 24:13247. https:\/\/doi.org\/10.3390\/ijms241713247","journal-title":"Int J Mol Sci"},{"key":"10718_CR25","doi-asserted-by":"publisher","first-page":"316","DOI":"10.1016\/j.cocis.2012.10.003","volume":"17","author":"D Danino","year":"2012","unstructured":"Danino D (2012) Cryo-TEM of soft molecular assemblies. Curr Opin Colloid Interface Sci 17:316\u2013329. https:\/\/doi.org\/10.1016\/j.cocis.2012.10.003","journal-title":"Curr Opin Colloid Interface Sci"},{"key":"10718_CR26","doi-asserted-by":"publisher","first-page":"5202","DOI":"10.1039\/C2LC41006K","volume":"12","author":"RT Davies","year":"2012","unstructured":"Davies RT, Kim J, Jang SC et al (2012) Microfluidic filtration system to isolate extracellular vesicles from blood. Lab Chip 12:5202\u20135210. https:\/\/doi.org\/10.1039\/C2LC41006K","journal-title":"Lab Chip"},{"key":"10718_CR27","doi-asserted-by":"publisher","first-page":"e12111","DOI":"10.1002\/jev2.12111","volume":"10","author":"RC de Abreu","year":"2021","unstructured":"de Abreu RC, Ramos CV, Becher C et al (2021) Exogenous loading of miRNAs into small extracellular vesicles. J Extracell Vesicles 10:e12111. https:\/\/doi.org\/10.1002\/jev2.12111","journal-title":"J Extracell Vesicles"},{"key":"10718_CR28","doi-asserted-by":"publisher","first-page":"e1835","DOI":"10.1002\/wnan.1835","volume":"15","author":"KP De Sousa","year":"2023","unstructured":"De Sousa KP, Rossi I, Abdullahi M et al (2023) Isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy. Wiley Interdiscip Rev Nanomed Nanobiotechnol 15:e1835. https:\/\/doi.org\/10.1002\/wnan.1835","journal-title":"Wiley Interdiscip Rev Nanomed Nanobiotechnol"},{"key":"10718_CR29","doi-asserted-by":"publisher","unstructured":"Dias MKHM, Jayathilaka EHTT, Jayasinghe JNC et al (2025) Exploring the proteomic landscape and immunomodulatory functions of edwardsiella piscicida derived extracellular Vesicles. 35. https:\/\/doi.org\/10.4014\/jmb.2410.10001","DOI":"10.4014\/jmb.2410.10001"},{"key":"10718_CR30","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1186\/s40779-023-00472-w","volume":"10","author":"J-Y Ding","year":"2023","unstructured":"Ding J-Y, Chen M-J, Wu L-F et al (2023) Mesenchymal stem cell-derived extracellular vesicles in skin wound healing: roles, opportunities and challenges. Mil Med Res 10:36. https:\/\/doi.org\/10.1186\/s40779-023-00472-w","journal-title":"Mil Med Res"},{"key":"10718_CR31","doi-asserted-by":"publisher","first-page":"922","DOI":"10.3390\/biology14080922","volume":"14","author":"A Dobrzycka-Krahel","year":"2025","unstructured":"Dobrzycka-Krahel A, Ste\u0107 A, Czyrski GS et al (2025) Characterization of Small Extracellular Vesicles Isolated from Aurelia aurita. Biology 14:922. https:\/\/doi.org\/10.3390\/biology14080922","journal-title":"Biology"},{"key":"10718_CR32","doi-asserted-by":"publisher","first-page":"780","DOI":"10.1016\/j.nano.2011.04.003","volume":"7","author":"RA Dragovic","year":"2011","unstructured":"Dragovic RA, Gardiner C, Brooks AS et al (2011) Sizing and phenotyping of cellular vesicles using Nanoparticle Tracking Analysis. Nanomed Nanotechnol Biol Med 7:780\u2013788. https:\/\/doi.org\/10.1016\/j.nano.2011.04.003","journal-title":"Nanomed Nanotechnol Biol Med"},{"key":"10718_CR33","doi-asserted-by":"publisher","first-page":"e0215324","DOI":"10.1371\/journal.pone.0215324","volume":"14","author":"P Duong","year":"2019","unstructured":"Duong P, Chung A, Bouchareychas L, Raffai RL (2019) Cushioned-Density Gradient Ultracentrifugation (C-DGUC) improves the isolation efficiency of extracellular vesicles. PLoS ONE 14:e0215324. https:\/\/doi.org\/10.1371\/journal.pone.0215324","journal-title":"PLoS ONE"},{"key":"10718_CR34","doi-asserted-by":"publisher","first-page":"756","DOI":"10.1002\/cyto.a.22494","volume":"85","author":"U Erdbr\u00fcgger","year":"2014","unstructured":"Erdbr\u00fcgger U, Rudy CK, Etter E M, et al (2014) Imaging flow cytometry elucidates limitations of microparticle analysis by conventional flow cytometry. Cytometry A 85:756\u2013770. https:\/\/doi.org\/10.1002\/cyto.a.22494","journal-title":"Cytometry A"},{"key":"10718_CR35","doi-asserted-by":"publisher","first-page":"113801","DOI":"10.1016\/j.addr.2021.05.011","volume":"175","author":"M Escud\u00e9","year":"2021","unstructured":"Escud\u00e9 M, de Castilla P, Tong L, Huang C et al (2021) Extracellular vesicles as a drug delivery system: A systematic review of preclinical studies. Adv Drug Deliv Rev 175:113801. https:\/\/doi.org\/10.1016\/j.addr.2021.05.011","journal-title":"Adv Drug Deliv Rev"},{"key":"10718_CR36","doi-asserted-by":"publisher","first-page":"736","DOI":"10.1038\/ki.2009.550","volume":"77","author":"P Fern\u00e1ndez-Llama","year":"2010","unstructured":"Fern\u00e1ndez-Llama P, Khositseth S, Gonzales PA et al (2010) Tamm-Horsfall protein and urinary exosome isolation. Kidney Int 77:736\u2013742. https:\/\/doi.org\/10.1038\/ki.2009.550","journal-title":"Kidney Int"},{"key":"10718_CR37","doi-asserted-by":"publisher","first-page":"10510","DOI":"10.3390\/ijms221910510","volume":"22","author":"D Fortunato","year":"2021","unstructured":"Fortunato D, Mladenovi\u0107 D, Criscuoli M et al (2021) Opportunities and Pitfalls of Fluorescent Labeling Methodologies for Extracellular Vesicle Profiling on High-Resolution Single-Particle Platforms. Int J Mol Sci 22:10510. https:\/\/doi.org\/10.3390\/ijms221910510","journal-title":"Int J Mol Sci"},{"key":"10718_CR38","doi-asserted-by":"publisher","first-page":"32945","DOI":"10.3402\/jev.v5.32945","volume":"5","author":"C Gardiner","year":"2016","unstructured":"Gardiner C, Di Vizio D, Sahoo S et al (2016) Techniques used for the isolation and characterization of extracellular vesicles: results of a worldwide survey. J Extracell Vesicles 5:32945. https:\/\/doi.org\/10.3402\/jev.v5.32945","journal-title":"J Extracell Vesicles"},{"key":"10718_CR39","doi-asserted-by":"crossref","unstructured":"Gargano P, Picciotto S, Paterna A et al (2025) Nanoalgosomes from tetraselmis chuii: microalgal extracellular vesicles for UV protection, anti-aging, and skin depigmentation. 2025.07.25.666736","DOI":"10.1101\/2025.07.25.666736"},{"key":"10718_CR40","doi-asserted-by":"publisher","first-page":"111209","DOI":"10.1016\/j.humimm.2024.111209","volume":"86","author":"H Ghaneialvar","year":"2025","unstructured":"Ghaneialvar H, Jahani S, Hashemi E et al (2025) Combining anti-checkpoint immunotherapies and cancer vaccines as a novel strategy in oncological therapy: A review. Hum Immunol 86:111209. https:\/\/doi.org\/10.1016\/j.humimm.2024.111209","journal-title":"Hum Immunol"},{"key":"10718_CR41","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1016\/j.jim.2011.06.024","volume":"371","author":"R Grant","year":"2011","unstructured":"Grant R, Ansa-Addo E, Stratton D et al (2011) A filtration-based protocol to isolate human Plasma Membrane-derived Vesicles and exosomes from blood plasma. J Immunol Methods 371:143\u2013151. https:\/\/doi.org\/10.1016\/j.jim.2011.06.024","journal-title":"J Immunol Methods"},{"key":"10718_CR42","doi-asserted-by":"publisher","first-page":"216784","DOI":"10.1016\/j.ccr.2025.216784","volume":"541","author":"H Gui","year":"2025","unstructured":"Gui H, Wang W, Xue Y et al (2025) Extracellular vesicles as nanomaterial carriers: Endowing superior targeting capability and biosafety. Coord Chem Rev 541:216784. https:\/\/doi.org\/10.1016\/j.ccr.2025.216784","journal-title":"Coord Chem Rev"},{"key":"10718_CR43","doi-asserted-by":"publisher","first-page":"5237","DOI":"10.1038\/srep05237","volume":"4","author":"SE Headland","year":"2014","unstructured":"Headland SE, Jones HR, D\u2019Sa ASV et al (2014) Cutting-Edge Analysis of Extracellular Microparticles using ImageStreamX Imaging Flow Cytometry. Sci Rep 4:5237. https:\/\/doi.org\/10.1038\/srep05237","journal-title":"Sci Rep"},{"key":"10718_CR44","doi-asserted-by":"publisher","first-page":"2684","DOI":"10.2174\/0113892010323495241016085000","volume":"26","author":"MB Heydari","year":"2025","unstructured":"Heydari MB, Ghanbari-Movahed Z, Heydari M, Farzaei MH (2025) The Exosome and its Application in Skin Wound Healing: A Systematic Review on In vitro Studies. Curr Pharm Biotechnol 26:2684\u20132696. https:\/\/doi.org\/10.2174\/0113892010323495241016085000","journal-title":"Curr Pharm Biotechnol"},{"key":"10718_CR45","doi-asserted-by":"publisher","unstructured":"Heydari MB, Ghanbari-Movahed Z, Heydari M, Farzaei MH (2025) The exosome and its application in skin wound healing: a systematic review on In vitro studies. Curr Pharm Biotechnol 26:2684\u20132696. https:\/\/doi.org\/10.2174\/0113892010323495241016085000","DOI":"10.2174\/0113892010323495241016085000"},{"key":"10718_CR46","doi-asserted-by":"publisher","first-page":"1225","DOI":"10.1038\/ki.2013.422","volume":"85","author":"MC Hogan","year":"2014","unstructured":"Hogan MC, Johnson KL, Zenka RM et al (2014) Subfractionation, characterization, and in-depth proteomic analysis of glomerular membrane vesicles in human urine. Kidney Int 85:1225\u20131237. https:\/\/doi.org\/10.1038\/ki.2013.422","journal-title":"Kidney Int"},{"key":"10718_CR47","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1007\/s11051-008-9435-7","volume":"10","author":"CM Hoo","year":"2008","unstructured":"Hoo CM, Starostin N, West P, Mecartney ML (2008) A comparison of atomic force microscopy (AFM) and dynamic light scattering (DLS) methods to characterize nanoparticle size distributions. J Nanoparticle Res 10:89\u201396. https:\/\/doi.org\/10.1007\/s11051-008-9435-7","journal-title":"J Nanoparticle Res"},{"key":"10718_CR48","doi-asserted-by":"publisher","first-page":"392","DOI":"10.3390\/mi10060392","volume":"10","author":"FS Iliescu","year":"2019","unstructured":"Iliescu FS, Vrta\u010dnik D, Neuzil P, Iliescu C (2019) Microfluidic Technology for Clinical Applications of Exosomes. Micromachines 10:392. https:\/\/doi.org\/10.3390\/mi10060392","journal-title":"Micromachines"},{"key":"10718_CR49","doi-asserted-by":"publisher","first-page":"e83680","DOI":"10.1371\/journal.pone.0083680","volume":"8","author":"L Issman","year":"2013","unstructured":"Issman L, Brenner B, Talmon Y, Aharon A (2013) Cryogenic Transmission Electron Microscopy Nanostructural Study of Shed Microparticles. PLoS ONE 8:e83680. https:\/\/doi.org\/10.1371\/journal.pone.0083680","journal-title":"PLoS ONE"},{"key":"10718_CR50","doi-asserted-by":"publisher","first-page":"736","DOI":"10.14348\/molcells.2021.2167","volume":"44","author":"B Jang","year":"2021","unstructured":"Jang B, Chung H, Jung H et al (2021) Extracellular Vesicles from Korean Codium fragile and Sargassum fusiforme Negatively Regulate Melanin Synthesis. Mol Cells 44:736\u2013745. https:\/\/doi.org\/10.14348\/molcells.2021.2167","journal-title":"Mol Cells"},{"key":"10718_CR51","doi-asserted-by":"publisher","first-page":"196","DOI":"10.1016\/j.fsi.2022.07.076","volume":"128","author":"EHTT Jayathilaka","year":"2022","unstructured":"Jayathilaka EHTT, Edirisinghe SL, Lee J et al (2022) Isolation and characterization of plasma-derived exosomes from olive flounder (Paralichthys olivaceus) and their wound healing and regeneration activities. Fish Shellfish Immunol 128:196\u2013205. https:\/\/doi.org\/10.1016\/j.fsi.2022.07.076","journal-title":"Fish Shellfish Immunol"},{"key":"10718_CR53","doi-asserted-by":"publisher","first-page":"592","DOI":"10.3390\/md19110592","volume":"19","author":"S-H Jo","year":"2021","unstructured":"Jo S-H, Kim S-H, Kim C, Park S-H (2021) Characterization of Marine Organism Extracellular Matrix-Anchored Extracellular Vesicles and Their Biological Effect on the Alleviation of Pro-Inflammatory Cytokines. Mar Drugs 19:592. https:\/\/doi.org\/10.3390\/md19110592","journal-title":"Mar Drugs"},{"key":"10718_CR52","doi-asserted-by":"publisher","first-page":"0154","DOI":"10.34133\/bmr.0154","volume":"29","author":"S-H Jo","year":"2025","unstructured":"Jo S-H, Kim S-H, Heo SC et al (2025) Exploring the Therapeutic Potential of Extracellular Vesicles Anchored to the Sea Cucumber Extracellular Matrix for Treating Atopic Dermatitis. Biomater Res 29:0154. https:\/\/doi.org\/10.34133\/bmr.0154","journal-title":"Biomater Res"},{"key":"10718_CR54","doi-asserted-by":"publisher","first-page":"100151","DOI":"10.1016\/j.mocell.2024.100151","volume":"47","author":"H Jung","year":"2024","unstructured":"Jung H, Jung Y, Seo J et al (2024) Roles of extracellular vesicles from mesenchymal stem cells in regeneration. Mol Cells 47:100151. https:\/\/doi.org\/10.1016\/j.mocell.2024.100151","journal-title":"Mol Cells"},{"key":"10718_CR55","doi-asserted-by":"publisher","first-page":"eaau6977","DOI":"10.1126\/science.aau6977","volume":"367","author":"R Kalluri","year":"2020","unstructured":"Kalluri R, LeBleu VS (2020) The biology, function, and biomedical applications of exosomes. Science 367:eaau6977. https:\/\/doi.org\/10.1126\/science.aau6977","journal-title":"Science"},{"key":"10718_CR56","doi-asserted-by":"publisher","first-page":"586","DOI":"10.1093\/schbul\/sbab009","volume":"47","author":"S Kano","year":"2021","unstructured":"Kano S, Yang K, Sawa A (2021) Making Sense of Extracellular Vesicles in Body Fluids: Promise and Challenge. Schizophr Bull 47:586\u2013587. https:\/\/doi.org\/10.1093\/schbul\/sbab009","journal-title":"Schizophr Bull"},{"key":"10718_CR57","doi-asserted-by":"publisher","first-page":"709","DOI":"10.1007\/s12015-024-10835-y","volume":"21","author":"MA Khalilzad","year":"2025","unstructured":"Khalilzad MA, Mohammadi J, Amirsaadat S et al (2025a) Elevating Dermatology Beyond Aesthetics: Perinatal-Derived Advancements for Rejuvenation, Alopecia Strategies, Scar Therapies, and Progressive Wound Healing. Stem Cell Rev Rep 21:709\u2013729. https:\/\/doi.org\/10.1007\/s12015-024-10835-y","journal-title":"Stem Cell Rev Rep"},{"key":"10718_CR58","doi-asserted-by":"publisher","first-page":"351","DOI":"10.1007\/s12015-024-10822-3","volume":"21","author":"MA Khalilzad","year":"2025","unstructured":"Khalilzad MA, Mohammadi J, Najafi S et al (2025b) Harnessing the Anti-Inflammatory Effects of Perinatal Tissue Derived Therapies for the Treatment of Inflammatory Skin Diseases: A Comprehensive Review. Stem Cell Rev Rep 21:351\u2013371. https:\/\/doi.org\/10.1007\/s12015-024-10822-3","journal-title":"Stem Cell Rev Rep"},{"key":"10718_CR59","doi-asserted-by":"publisher","first-page":"1075","DOI":"10.1038\/jid.2013.478","volume":"134","author":"N-H Kim","year":"2014","unstructured":"Kim N-H, Choi S-H, Kim C-H et al (2014) Reduced MiR-675 in Exosome in H19 RNA-Related Melanogenesis via MITF as a Direct Target. J Invest Dermatol 134:1075\u20131082. https:\/\/doi.org\/10.1038\/jid.2013.478","journal-title":"J Invest Dermatol"},{"key":"10718_CR60","doi-asserted-by":"publisher","first-page":"533","DOI":"10.1016\/j.jconrel.2022.05.027","volume":"347","author":"I Kimiz-Gebologlu","year":"2022","unstructured":"Kimiz-Gebologlu I, Oncel SS (2022) Exosomes: Large-scale production, isolation, drug loading efficiency, and biodistribution and uptake. J Controlled Release 347:533\u2013543. https:\/\/doi.org\/10.1016\/j.jconrel.2022.05.027","journal-title":"J Controlled Release"},{"key":"10718_CR61","doi-asserted-by":"publisher","first-page":"8545347","DOI":"10.1155\/2018\/8545347","volume":"2018","author":"EA Konoshenko MYu, Lekchnov","year":"2018","unstructured":"Konoshenko MYu, Lekchnov EA, Vlassov AV, Laktionov PP (2018) Isolation of Extracellular Vesicles: General Methodologies and Latest Trends. BioMed Res Int 2018:8545347. https:\/\/doi.org\/10.1155\/2018\/8545347","journal-title":"BioMed Res Int"},{"key":"10718_CR62","doi-asserted-by":"publisher","first-page":"580","DOI":"10.1038\/s41419-022-05034-x","volume":"13","author":"M Kou","year":"2022","unstructured":"Kou M, Huang L, Yang J et al (2022) Mesenchymal stem cell-derived extracellular vesicles for immunomodulation and regeneration: a next generation therapeutic tool? Cell Death Dis 13:580. https:\/\/doi.org\/10.1038\/s41419-022-05034-x","journal-title":"Cell Death Dis"},{"key":"10718_CR63","doi-asserted-by":"publisher","unstructured":"Kumar A, Ahmad A, Vyawahare A, Khan R (2020) Membrane trafficking and subcellular drug targeting pathways. Front Pharmacol 11. https:\/\/doi.org\/10.3389\/fphar.2020.00629","DOI":"10.3389\/fphar.2020.00629"},{"key":"10718_CR64","doi-asserted-by":"publisher","first-page":"e00808","DOI":"10.1002\/mbo3.808","volume":"8","author":"YM Kwon","year":"2019","unstructured":"Kwon YM, Patra AK, Chiura HX, Kim S-J (2019) Production of extracellular vesicles with light-induced proton pump activity by proteorhodopsin-containing marine bacteria. MicrobiologyOpen 8:e00808. https:\/\/doi.org\/10.1002\/mbo3.808","journal-title":"MicrobiologyOpen"},{"key":"10718_CR65","doi-asserted-by":"publisher","first-page":"1110","DOI":"10.1002\/ijc.35463","volume":"157","author":"O Langselius","year":"2025","unstructured":"Langselius O, Rumgay H, de Vries E et al (2025) Global burden of cutaneous melanoma incidence attributable to ultraviolet radiation in 2022. Int J Cancer 157:1110\u20131119. https:\/\/doi.org\/10.1002\/ijc.35463","journal-title":"Int J Cancer"},{"key":"10718_CR66","doi-asserted-by":"publisher","first-page":"105981","DOI":"10.1016\/j.jff.2023.105981","volume":"112","author":"HJ Lee","year":"2024","unstructured":"Lee HJ, Shin K-W, Lee SJ et al (2024) Immunomodulation by extracellular vesicle-like nanoparticles from marine macroalgae Sargassum fusiforme: Enhancing Type 1 T helper and cytotoxic T lymphocyte-mediated immune responses. J Funct Foods 112:105981. https:\/\/doi.org\/10.1016\/j.jff.2023.105981","journal-title":"J Funct Foods"},{"key":"10718_CR67","doi-asserted-by":"publisher","first-page":"46224","DOI":"10.1038\/srep46224","volume":"7","author":"L-G Liang","year":"2017","unstructured":"Liang L-G, Kong M-Q, Zhou S et al (2017) An integrated double-filtration microfluidic device for isolation, enrichment and quantification of urinary extracellular vesicles for detection of bladder cancer. Sci Rep 7:46224. https:\/\/doi.org\/10.1038\/srep46224","journal-title":"Sci Rep"},{"key":"10718_CR68","doi-asserted-by":"publisher","first-page":"461773","DOI":"10.1016\/j.chroma.2020.461773","volume":"1636","author":"T Liangsupree","year":"2021","unstructured":"Liangsupree T, Multia E, Riekkola M-L (2021) Modern isolation and separation techniques for extracellular vesicles. J Chromatogr A 1636:461773. https:\/\/doi.org\/10.1016\/j.chroma.2020.461773","journal-title":"J Chromatogr A"},{"key":"10718_CR70","doi-asserted-by":"publisher","first-page":"6623","DOI":"10.3390\/s100706623","volume":"10","author":"K-K Liu","year":"2010","unstructured":"Liu K-K, Wu R-G, Chuang Y-J et al (2010) Microfluidic Systems for Biosensing. Sensors 10:6623\u20136661. https:\/\/doi.org\/10.3390\/s100706623","journal-title":"Sensors"},{"key":"10718_CR69","doi-asserted-by":"publisher","first-page":"10712","DOI":"10.1021\/acsnano.7b04878","volume":"11","author":"F Liu","year":"2017","unstructured":"Liu F, Vermesh O, Mani V et al (2017) The Exosome Total Isolation Chip. ACS Nano 11:10712\u201310723. https:\/\/doi.org\/10.1021\/acsnano.7b04878","journal-title":"ACS Nano"},{"key":"10718_CR71","doi-asserted-by":"publisher","first-page":"159","DOI":"10.1016\/j.jdermsci.2019.02.001","volume":"93","author":"Y Liu","year":"2019","unstructured":"Liu Y, Xue L, Gao H et al (2019) Exosomal miRNA derived from keratinocytes regulates pigmentation in melanocytes. J Dermatol Sci 93:159\u2013167. https:\/\/doi.org\/10.1016\/j.jdermsci.2019.02.001","journal-title":"J Dermatol Sci"},{"key":"10718_CR72","doi-asserted-by":"publisher","unstructured":"Ljungstr\u00f6m M, Oltra E (2025) Methods for extracellular vesicle isolation: relevance for encapsulated miRNAs in disease diagnosis and treatment. Genes 16. https:\/\/doi.org\/10.3390\/genes16030330","DOI":"10.3390\/genes16030330"},{"key":"10718_CR73","doi-asserted-by":"publisher","first-page":"1528109","DOI":"10.1080\/20013078.2018.1528109","volume":"7","author":"A-K Ludwig","year":"2018","unstructured":"Ludwig A-K, De Miroschedji K, Doeppner TR et al (2018) Precipitation with polyethylene glycol followed by washing and pelleting by ultracentrifugation enriches extracellular vesicles from tissue culture supernatants in small and large scales. J Extracell Vesicles 7:1528109. https:\/\/doi.org\/10.1080\/20013078.2018.1528109","journal-title":"J Extracell Vesicles"},{"key":"10718_CR74","doi-asserted-by":"publisher","first-page":"1541396","DOI":"10.1080\/20013078.2018.1541396","volume":"7","author":"RP McNamara","year":"2018","unstructured":"McNamara RP, Caro-Vegas CP, Costantini LM et al (2018) Large-scale, cross-flow based isolation of highly pure and endocytosis-competent extracellular vesicles. J Extracell Vesicles 7:1541396. https:\/\/doi.org\/10.1080\/20013078.2018.1541396","journal-title":"J Extracell Vesicles"},{"key":"10718_CR75","doi-asserted-by":"publisher","first-page":"50","DOI":"10.3390\/bios13010050","volume":"13","author":"A Meggiolaro","year":"2023","unstructured":"Meggiolaro A, Moccia V, Brun P et al (2023) Microfluidic Strategies for Extracellular Vesicle Isolation: Towards Clinical Applications. Biosensors 13:50. https:\/\/doi.org\/10.3390\/bios13010050","journal-title":"Biosensors"},{"key":"10718_CR76","doi-asserted-by":"publisher","first-page":"84","DOI":"10.1002\/prca.200800093","volume":"4","author":"ML Merchant","year":"2010","unstructured":"Merchant ML, Powell DW, Wilkey DW et al (2010) Microfiltration isolation of human urinary exosomes for characterization by MS. Proteom \u2013 Clin Appl 4:84\u201396. https:\/\/doi.org\/10.1002\/prca.200800093","journal-title":"Proteom \u2013 Clin Appl"},{"key":"10718_CR77","doi-asserted-by":"publisher","unstructured":"Minakata K, Taguchi M, Tame A et al (2025) Platelet-like anucleate cell fragments mediate wound healing and immune response in the sea star Patiria pectinifera. J Immunol Baltim Md 1950 vkaf246. https:\/\/doi.org\/10.1093\/jimmun\/vkaf246","DOI":"10.1093\/jimmun\/vkaf246"},{"key":"10718_CR78","doi-asserted-by":"publisher","first-page":"rbaf072","DOI":"10.1093\/rb\/rbaf072","volume":"12","author":"D Mo","year":"2025","unstructured":"Mo D, Zheng W, Gao Z et al (2025) Biocompatible exosomes derived from Pinctada martensii mucus for therapeutic melanin regulation via \u03b1-MSH\/NF-\u03baB\/MITF pathway. Regen Biomater 12:rbaf072. https:\/\/doi.org\/10.1093\/rb\/rbaf072","journal-title":"Regen Biomater"},{"key":"10718_CR79","doi-asserted-by":"publisher","first-page":"102893","DOI":"10.1016\/j.algal.2022.102893","volume":"68","author":"BR Moreira","year":"2022","unstructured":"Moreira BR, Vega J, Sisa ADA et al (2022) Antioxidant and anti-photoaging properties of red marine macroalgae: Screening of bioactive molecules for cosmeceutical applications. Algal Res 68:102893. https:\/\/doi.org\/10.1016\/j.algal.2022.102893","journal-title":"Algal Res"},{"key":"10718_CR80","doi-asserted-by":"publisher","unstructured":"Moros M, Fergola E, Marchesano V et al (2021) The aquatic invertebrate hydra vulgaris releases molecular messages through extracellular vesicles. Front Cell Dev Biol 9. https:\/\/doi.org\/10.3389\/fcell.2021.788117","DOI":"10.3389\/fcell.2021.788117"},{"key":"10718_CR81","doi-asserted-by":"publisher","first-page":"174","DOI":"10.1007\/s11274-024-03963-7","volume":"40","author":"LM Mu\u00f1oz-Echeverri","year":"2024","unstructured":"Mu\u00f1oz-Echeverri LM, Benavides-L\u00f3pez S, Geiger O et al (2024) Bacterial extracellular vesicles: biotechnological perspective for enhanced productivity. World J Microbiol Biotechnol 40:174. https:\/\/doi.org\/10.1007\/s11274-024-03963-7","journal-title":"World J Microbiol Biotechnol"},{"key":"10718_CR82","doi-asserted-by":"publisher","first-page":"416","DOI":"10.3390\/cancers10110416","volume":"10","author":"A Nanou","year":"2018","unstructured":"Nanou A, Crespo M, Flohr P et al (2018) Scanning Electron Microscopy of Circulating Tumor Cells and Tumor-Derived Extracellular Vesicles. Cancers 10:416. https:\/\/doi.org\/10.3390\/cancers10110416","journal-title":"Cancers"},{"key":"10718_CR83","doi-asserted-by":"publisher","first-page":"282","DOI":"10.1186\/s12964-024-01660-4","volume":"22","author":"A N\u00e9meth","year":"2024","unstructured":"N\u00e9meth A, B\u00e1nyai GL, Dobos NK et al (2024) Extracellular vesicles promote migration despite BRAF inhibitor treatment in malignant melanoma cells. Cell Commun Signal 22:282. https:\/\/doi.org\/10.1186\/s12964-024-01660-4","journal-title":"Cell Commun Signal"},{"key":"10718_CR84","doi-asserted-by":"publisher","first-page":"36","DOI":"10.3390\/fishes7010036","volume":"7","author":"C Nikapitiya","year":"2022","unstructured":"Nikapitiya C, Jayathilaka EHTT, Edirisinghe SL et al (2022) Isolation and Characterization of Plasma-Derived Exosomes from the Marine Fish Rock Bream (Oplegnathus fasciatus) by Two Isolation Techniques. Fishes 7:36. https:\/\/doi.org\/10.3390\/fishes7010036","journal-title":"Fishes"},{"key":"10718_CR85","doi-asserted-by":"publisher","first-page":"e0186534","DOI":"10.1371\/journal.pone.0186534","volume":"12","author":"Z Niu","year":"2017","unstructured":"Niu Z, Pang RTK, Liu W et al (2017) Polymer-based precipitation preserves biological activities of extracellular vesicles from an endometrial cell line. PLoS ONE 12:e0186534. https:\/\/doi.org\/10.1371\/journal.pone.0186534","journal-title":"PLoS ONE"},{"key":"10718_CR86","doi-asserted-by":"publisher","first-page":"301","DOI":"10.1002\/cyto.a.22255","volume":"83A","author":"JP Nolan","year":"2013","unstructured":"Nolan JP, Stoner SA (2013) A trigger channel threshold artifact in nanoparticle analysis. Cytometry A 83A:301\u2013305. https:\/\/doi.org\/10.1002\/cyto.a.22255","journal-title":"Cytometry A"},{"key":"10718_CR87","doi-asserted-by":"publisher","first-page":"287","DOI":"10.1007\/978-1-4939-9145-7_18","volume-title":"Target Identification and Validation in Drug Discovery: Methods and Protocols","author":"JZ Nordin","year":"2019","unstructured":"Nordin JZ, Bostancioglu RB, Corso G, EL Andaloussi S (2019) Tangential Flow Filtration with or Without Subsequent Bind-Elute Size Exclusion Chromatography for Purification of Extracellular Vesicles. In: Moll J, Carotta S (eds) Target Identification and Validation in Drug Discovery: Methods and Protocols. Springer, New York, NY, pp 287\u2013299"},{"key":"10718_CR88","doi-asserted-by":"publisher","first-page":"10267","DOI":"10.3390\/ijms241210267","volume":"24","author":"M Nowak","year":"2023","unstructured":"Nowak M, G\u00f3rczy\u0144ska J, Ko\u0142odzi\u0144ska K et al (2023) Extracellular Vesicles as Drug Transporters. Int J Mol Sci 24:10267. https:\/\/doi.org\/10.3390\/ijms241210267","journal-title":"Int J Mol Sci"},{"key":"10718_CR89","doi-asserted-by":"publisher","first-page":"585","DOI":"10.1038\/s41580-020-0251-y","volume":"21","author":"K O\u2019Brien","year":"2020","unstructured":"O\u2019Brien K, Breyne K, Ughetto S et al (2020) RNA delivery by extracellular vesicles in mammalian cells and its applications. Nat Rev Mol Cell Biol 21:585\u2013606. https:\/\/doi.org\/10.1038\/s41580-020-0251-y","journal-title":"Nat Rev Mol Cell Biol"},{"key":"10718_CR90","doi-asserted-by":"publisher","first-page":"e70047","DOI":"10.1002\/jex2.70047","volume":"4","author":"K Otani","year":"2025","unstructured":"Otani K, Fujioka Y, Okada M, Yamawaki H (2025) Development of an Effective Isolation Method for Plasma Extracellular Vesicles Excluding Lipoproteins by Precipitation With Polyethylene Glycol. J Extracell Biol 4:e70047. https:\/\/doi.org\/10.1002\/jex2.70047","journal-title":"J Extracell Biol"},{"key":"10718_CR91","doi-asserted-by":"publisher","first-page":"2583","DOI":"10.1007\/s11051-014-2583-z","volume":"16","author":"V Palmieri","year":"2014","unstructured":"Palmieri V, Lucchetti D, Gatto I et al (2014) Dynamic light scattering for the characterization and counting of extracellular vesicles: a powerful noninvasive tool. J Nanoparticle Res 16:2583. https:\/\/doi.org\/10.1007\/s11051-014-2583-z","journal-title":"J Nanoparticle Res"},{"key":"10718_CR92","doi-asserted-by":"publisher","first-page":"e0178601","DOI":"10.1371\/journal.pone.0178601","volume":"12","author":"LJ Pearson","year":"2017","unstructured":"Pearson LJ, Klaharn I, -yanut, Thongsawang B et al (2017) Multiple extracellular vesicle types in peritoneal dialysis effluent are prominent and contain known biomarkers. PLoS ONE 12:e0178601. https:\/\/doi.org\/10.1371\/journal.pone.0178601","journal-title":"PLoS ONE"},{"key":"10718_CR93","doi-asserted-by":"publisher","first-page":"1199","DOI":"10.1007\/s10695-023-01251-z","volume":"49","author":"C Pelyhe","year":"2023","unstructured":"Pelyhe C, Sturve J (2023) Isolation and characterization of the morphology, size and particle number of rainbow trout (Oncorhynchus mykiss) and zebrafish (Danio rerio) cell line derived large and small extracellular vesicles. Fish Physiol Biochem 49:1199\u20131214. https:\/\/doi.org\/10.1007\/s10695-023-01251-z","journal-title":"Fish Physiol Biochem"},{"key":"10718_CR94","doi-asserted-by":"publisher","unstructured":"Phan TH, Divakarla SK, Yeo JH et al (2021) New multiscale characterization methodology for effective determination of isolation\u2013structure\u2013function relationship of extracellular vesicles. Front Bioeng Biotechnol 9. https:\/\/doi.org\/10.3389\/fbioe.2021.669537","DOI":"10.3389\/fbioe.2021.669537"},{"key":"10718_CR95","doi-asserted-by":"publisher","first-page":"2917","DOI":"10.1039\/D0BM01696A","volume":"9","author":"S Picciotto","year":"2021","unstructured":"Picciotto S, Barone ME, Fierli D et al (2021) Isolation of extracellular vesicles from microalgae: towards the production of sustainable and natural nanocarriers of bioactive compounds. Biomater Sci 9:2917\u20132930. https:\/\/doi.org\/10.1039\/D0BM01696A","journal-title":"Biomater Sci"},{"key":"10718_CR97","doi-asserted-by":"publisher","first-page":"662","DOI":"10.1016\/j.jid.2021.07.167","volume":"142","author":"A Prasai","year":"2022","unstructured":"Prasai A, Jay JW, Jupiter D et al (2022) Role of Exosomes in Dermal Wound Healing: A Systematic Review. J Invest Dermatol 142:662\u2013678e8. https:\/\/doi.org\/10.1016\/j.jid.2021.07.167","journal-title":"J Invest Dermatol"},{"key":"10718_CR98","doi-asserted-by":"publisher","unstructured":"Price B (2002) Electron Microscopy, Second Edition, John J. Bozzola and Lonnie D. Russell. Jones and Bartlett Publishers, Inc., Sudbury, MA, 1999, 670 pages. ISBN 0-7637-0192-0. Microsc Microanal 8:365\u2013366. https:\/\/doi.org\/10.1017\/S1431927602029975","DOI":"10.1017\/S1431927602029975"},{"key":"10718_CR99","doi-asserted-by":"publisher","first-page":"106","DOI":"10.1186\/s12951-025-03194-4","volume":"23","author":"Y Qin","year":"2025","unstructured":"Qin Y, Chen X, Bao L et al (2025) Lipid metabolism of apoptotic vesicles accelerates cutaneous wound healing by modulating macrophage function. J Nanobiotechnol 23:106. https:\/\/doi.org\/10.1186\/s12951-025-03194-4","journal-title":"J Nanobiotechnol"},{"key":"10718_CR100","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-662-13553-2","volume-title":"Transmission Electron Microscopy","author":"L Reimer","year":"1984","unstructured":"Reimer L (1984) Transmission Electron Microscopy. Springer, Berlin, Heidelberg"},{"key":"10718_CR101","doi-asserted-by":"publisher","first-page":"1054","DOI":"10.1161\/ATVBAHA.111.244616","volume":"32","author":"S Robert","year":"2012","unstructured":"Robert S, Lacroix R, Poncelet P et al (2012) High-Sensitivity Flow Cytometry Provides Access to Standardized Measurement of Small-Size Microparticles\u2014Brief Report. Arterioscler Thromb Vasc Biol 32:1054\u20131058. https:\/\/doi.org\/10.1161\/ATVBAHA.111.244616","journal-title":"Arterioscler Thromb Vasc Biol"},{"key":"10718_CR102","doi-asserted-by":"publisher","first-page":"101715","DOI":"10.1016\/j.mtbio.2025.101715","volume":"32","author":"J Ruan","year":"2025","unstructured":"Ruan J, Xia Y, Ma Y et al (2025) Milk-derived exosomes as functional nanocarriers in wound healing: Mechanisms, applications, and future directions. Mater Today Bio 32:101715. https:\/\/doi.org\/10.1016\/j.mtbio.2025.101715","journal-title":"Mater Today Bio"},{"key":"10718_CR103","doi-asserted-by":"publisher","first-page":"1857","DOI":"10.3390\/biom11121857","volume":"11","author":"S Serrat\u00ec","year":"2021","unstructured":"Serrat\u00ec S, Palazzo A, Lapenna A et al (2021) Salting-Out Approach Is Worthy of Comparison with Ultracentrifugation for Extracellular Vesicle Isolation from Tumor and Healthy Models. Biomolecules 11:1857. https:\/\/doi.org\/10.3390\/biom11121857","journal-title":"Biomolecules"},{"key":"10718_CR104","doi-asserted-by":"publisher","first-page":"9937","DOI":"10.7150\/thno.45488","volume":"10","author":"S Shao","year":"2020","unstructured":"Shao S, Fang H, Li Q, Wang G (2020) Extracellular vesicles in Inflammatory Skin Disorders: from Pathophysiology to Treatment. Theranostics 10:9937\u20139955. https:\/\/doi.org\/10.7150\/thno.45488","journal-title":"Theranostics"},{"key":"10718_CR105","doi-asserted-by":"publisher","first-page":"12","DOI":"10.3390\/proteomes13010012","volume":"13","author":"M Singh","year":"2025","unstructured":"Singh M, Tiwari PK, Kashyap V, Kumar S (2025) Proteomics of Extracellular Vesicles: Recent Updates, Challenges and Limitations. Proteomes 13:12. https:\/\/doi.org\/10.3390\/proteomes13010012","journal-title":"Proteomes"},{"key":"10718_CR106","doi-asserted-by":"publisher","first-page":"146","DOI":"10.1016\/j.colsurfb.2011.05.013","volume":"87","author":"V Sokolova","year":"2011","unstructured":"Sokolova V, Ludwig A-K, Hornung S et al (2011) Characterisation of exosomes derived from human cells by nanoparticle tracking analysis and scanning electron microscopy. Colloids Surf B Biointerfaces 87:146\u2013150. https:\/\/doi.org\/10.1016\/j.colsurfb.2011.05.013","journal-title":"Colloids Surf B Biointerfaces"},{"key":"10718_CR107","doi-asserted-by":"publisher","first-page":"122604","DOI":"10.1016\/j.jchromb.2021.122604","volume":"1169","author":"J Stam","year":"2021","unstructured":"Stam J, Bartel S, Bischoff R, Wolters JC (2021) Isolation of extracellular vesicles with combined enrichment methods. J Chromatogr B 1169:122604. https:\/\/doi.org\/10.1016\/j.jchromb.2021.122604","journal-title":"J Chromatogr B"},{"key":"10718_CR108","doi-asserted-by":"publisher","first-page":"94","DOI":"10.1002\/cyto.a.10115","volume":"57A","author":"HB Steen","year":"2004","unstructured":"Steen HB (2004) Flow cytometer for measurement of the light scattering of viral and other submicroscopic particles. Cytometry A 57A:94\u201399. https:\/\/doi.org\/10.1002\/cyto.a.10115","journal-title":"Cytometry A"},{"key":"10718_CR109","doi-asserted-by":"publisher","unstructured":"Sun Y, Zhang S, Shen Y et al (2024) Therapeutic application of mesenchymal stem cell-derived exosomes in skin wound healing. Front Bioeng Biotechnol 12. https:\/\/doi.org\/10.3389\/fbioe.2024.1428793","DOI":"10.3389\/fbioe.2024.1428793"},{"key":"10718_CR110","doi-asserted-by":"publisher","first-page":"1153","DOI":"10.3390\/ijms18061153","volume":"18","author":"R Szatanek","year":"2017","unstructured":"Szatanek R, Baj-Krzyworzeka M, Zimoch J et al (2017) The Methods of Choice for Extracellular Vesicles (EVs) Characterization. Int J Mol Sci 18:1153. https:\/\/doi.org\/10.3390\/ijms18061153","journal-title":"Int J Mol Sci"},{"key":"10718_CR111","doi-asserted-by":"publisher","first-page":"2811","DOI":"10.7150\/thno.84665","volume":"13","author":"D Tan","year":"2023","unstructured":"Tan D, Zhu W, Liu L et al (2023) In situ formed scaffold with royal jelly-derived extracellular vesicles for wound healing. Theranostics 13:2811\u20132824. https:\/\/doi.org\/10.7150\/thno.84665","journal-title":"Theranostics"},{"key":"10718_CR112","doi-asserted-by":"publisher","first-page":"12295","DOI":"10.1038\/s41598-019-48181-6","volume":"9","author":"KE Thane","year":"2019","unstructured":"Thane KE, Davis AM, Hoffman AM (2019) Improved methods for fluorescent labeling and detection of single extracellular vesicles using nanoparticle tracking analysis. Sci Rep 9:12295. https:\/\/doi.org\/10.1038\/s41598-019-48181-6","journal-title":"Sci Rep"},{"key":"10718_CR113","doi-asserted-by":"publisher","first-page":"3221","DOI":"10.1002\/0471143030.cb0322s30","volume":"30","author":"C Th\u00e9ry","year":"2006","unstructured":"Th\u00e9ry C, Amigorena S, Raposo G, Clayton A (2006) Isolation and Characterization of Exosomes from Cell Culture Supernatants and Biological Fluids. Curr Protoc Cell Biol 30:3221\u201332229. https:\/\/doi.org\/10.1002\/0471143030.cb0322s30","journal-title":"Curr Protoc Cell Biol"},{"key":"10718_CR114","doi-asserted-by":"publisher","first-page":"694","DOI":"10.1016\/j.jconrel.2023.08.010","volume":"361","author":"G Tr\u00e9ton","year":"2023","unstructured":"Tr\u00e9ton G, Sayer C, Sch\u00fcrz M et al (2023) Quantitative and functional characterisation of extracellular vesicles after passive loading with hydrophobic or cholesterol-tagged small molecules. J Controlled Release 361:694\u2013716. https:\/\/doi.org\/10.1016\/j.jconrel.2023.08.010","journal-title":"J Controlled Release"},{"key":"10718_CR115","doi-asserted-by":"publisher","first-page":"12907","DOI":"10.3390\/ijms241612907","volume":"24","author":"AV Tupitsyna","year":"2023","unstructured":"Tupitsyna AV, Grigorieva AE, Soboleva SE et al (2023) Isolation of Extracellular Vesicles of Holothuria (Sea Cucumber Eupentacta fraudatrix). Int J Mol Sci 24:12907. https:\/\/doi.org\/10.3390\/ijms241612907","journal-title":"Int J Mol Sci"},{"key":"10718_CR96","doi-asserted-by":"publisher","first-page":"1182","DOI":"10.1111\/jth.12602","volume":"12","author":"E van der Pol","year":"2014","unstructured":"van der Pol E, Coumans Fa, Grootemaat W AE, et al (2014) Particle size distribution of exosomes and microvesicles determined by transmission electron microscopy, flow cytometry, nanoparticle tracking analysis, and resistive pulse sensing. J Thromb Haemost 12:1182\u20131192. https:\/\/doi.org\/10.1111\/jth.12602","journal-title":"J Thromb Haemost"},{"key":"10718_CR116","doi-asserted-by":"publisher","first-page":"1311","DOI":"10.1038\/nprot.2012.065","volume":"7","author":"EJ van der Vlist","year":"2012","unstructured":"van der Vlist EJ, Nolte-\u2019t Hoen ENM, Stoorvogel W et al (2012) Fluorescent labeling of nano-sized vesicles released by cells and subsequent quantitative and qualitative analysis by high-resolution flow cytometry. Nat Protoc 7:1311\u20131326. https:\/\/doi.org\/10.1038\/nprot.2012.065","journal-title":"Nat Protoc"},{"key":"10718_CR117","doi-asserted-by":"publisher","first-page":"990","DOI":"10.3390\/pharmaceutics17080990","volume":"17","author":"N Verma","year":"2025","unstructured":"Verma N, Arora S (2025) Navigating the Global Regulatory Landscape for Exosome-Based Therapeutics: Challenges, Strategies, and Future Directions. Pharmaceutics 17:990. https:\/\/doi.org\/10.3390\/pharmaceutics17080990","journal-title":"Pharmaceutics"},{"key":"10718_CR118","doi-asserted-by":"publisher","first-page":"894","DOI":"10.3390\/cancers15030894","volume":"15","author":"M Vitiello","year":"2023","unstructured":"Vitiello M, Mercatanti A, Podda MS et al (2023) A Network of MicroRNAs and mRNAs Involved in Melanosome Maturation and Trafficking Defines the Lower Response of Pigmentable Melanoma Cells to Targeted Therapy. Cancers 15:894. https:\/\/doi.org\/10.3390\/cancers15030894","journal-title":"Cancers"},{"key":"10718_CR119","doi-asserted-by":"publisher","first-page":"157","DOI":"10.1515\/cclm-2023-0610","volume":"62","author":"P Vychytilova-Faltejskova","year":"2024","unstructured":"Vychytilova-Faltejskova P, Vilmanova S, Pifkova L et al (2024) Optimized procedure for high-throughput transcriptome profiling of small extracellular vesicles isolated from low volume serum samples. Clin Chem Lab Med 62:157\u2013167. https:\/\/doi.org\/10.1515\/cclm-2023-0610","journal-title":"Clin Chem Lab Med"},{"key":"10718_CR121","doi-asserted-by":"publisher","first-page":"2300552","DOI":"10.1002\/advs.202300552","volume":"10","author":"L Wang","year":"2023","unstructured":"Wang L, Wang D, Ye Z, Xu J (2023) Engineering Extracellular Vesicles as Delivery Systems in Therapeutic Applications. Adv Sci 10:2300552. https:\/\/doi.org\/10.1002\/advs.202300552","journal-title":"Adv Sci"},{"key":"10718_CR120","doi-asserted-by":"publisher","first-page":"e20240011","DOI":"10.1002\/INMD.20240011","volume":"2","author":"J Wang","year":"2024","unstructured":"Wang J, Yuan S, Tu Y et al (2024) Extracellular vesicles in skin health, diseases, and aging. Interdiscip Med 2:e20240011. https:\/\/doi.org\/10.1002\/INMD.20240011","journal-title":"Interdiscip Med"},{"key":"10718_CR122","doi-asserted-by":"publisher","first-page":"19861","DOI":"10.3402\/jev.v2i0.19861","volume":"2","author":"J Webber","year":"2013","unstructured":"Webber J, Clayton A (2013) How pure are your vesicles? J Extracell Vesicles 2:19861. https:\/\/doi.org\/10.3402\/jev.v2i0.19861","journal-title":"J Extracell Vesicles"},{"key":"10718_CR123","doi-asserted-by":"publisher","first-page":"78","DOI":"10.3389\/fcell.2017.00078","volume":"5","author":"JA Welsh","year":"2017","unstructured":"Welsh JA, Holloway JA, Wilkinson JS, Englyst NA (2017) Extracellular Vesicle Flow Cytometry Analysis and Standardization. Front Cell Dev Biol 5:78. https:\/\/doi.org\/10.3389\/fcell.2017.00078","journal-title":"Front Cell Dev Biol"},{"key":"10718_CR124","doi-asserted-by":"publisher","first-page":"102664","DOI":"10.1016\/j.autrev.2020.102664","volume":"19","author":"PM Wong","year":"2020","unstructured":"Wong PM, Yang L, Yang L et al (2020) New insight into the role of exosomes in vitiligo. Autoimmun Rev 19:102664. https:\/\/doi.org\/10.1016\/j.autrev.2020.102664","journal-title":"Autoimmun Rev"},{"key":"10718_CR125","doi-asserted-by":"publisher","first-page":"1360","DOI":"10.1021\/acsnano.6b06131","volume":"11","author":"H-K Woo","year":"2017","unstructured":"Woo H-K, Sunkara V, Park J et al (2017) Exodisc for Rapid, Size-Selective, and Efficient Isolation and Analysis of Nanoscale Extracellular Vesicles from Biological Samples. ACS Nano 11:1360\u20131370. https:\/\/doi.org\/10.1021\/acsnano.6b06131","journal-title":"ACS Nano"},{"key":"10718_CR127","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1016\/j.bbrc.2022.09.115","volume":"634","author":"Z Wu","year":"2022","unstructured":"Wu Z, Ma L, Lin P et al (2022) Extracellular vesicles derived from Pinctada martensii mucus regulate skin inflammation via the NF-\u03baB\/NLRP3\/MAPK pathway. Biochem Biophys Res Commun 634:10\u201319. https:\/\/doi.org\/10.1016\/j.bbrc.2022.09.115","journal-title":"Biochem Biophys Res Commun"},{"key":"10718_CR126","doi-asserted-by":"publisher","first-page":"7667","DOI":"10.1021\/acsbiomaterials.4c01649","volume":"10","author":"Z Wu","year":"2024","unstructured":"Wu Z, He L, Yan L et al (2024) Hydrogels Treat Atopic Dermatitis by Transporting Marine-Derived miR-100-5p-Abundant Extracellular Vesicles. ACS Biomater Sci Eng 10:7667\u20137682. https:\/\/doi.org\/10.1021\/acsbiomaterials.4c01649","journal-title":"ACS Biomater Sci Eng"},{"key":"10718_CR128","doi-asserted-by":"publisher","first-page":"3400","DOI":"10.1021\/acsbiomaterials.4c02346","volume":"11","author":"R Xu","year":"2025","unstructured":"Xu R, Lu Y, Cai L, Zhang L (2025) Utilizing Extracellular Vesicles from Phaeodactylum tricornutum as a Novel Approach for Protecting the Skin from Oxidative Damage. ACS Biomater Sci Eng 11:3400\u20133415. https:\/\/doi.org\/10.1021\/acsbiomaterials.4c02346","journal-title":"ACS Biomater Sci Eng"},{"key":"10718_CR129","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1186\/s12929-025-01182-2","volume":"32","author":"NY Yeat","year":"2025","unstructured":"Yeat NY, Chen R-H (2025) Extracellular vesicles: biogenesis mechanism and impacts on tumor immune microenvironment. J Biomed Sci 32:85. https:\/\/doi.org\/10.1186\/s12929-025-01182-2","journal-title":"J Biomed Sci"},{"key":"10718_CR130","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1007\/978-1-0716-3203-1_5","volume-title":"Cell-Secreted Vesicles: Methods and Protocols","author":"R Yuan","year":"2023","unstructured":"Yuan R, Zhou Y, Arias GF, Dittmer DP (2023) Extracellular Vesicle Isolation by a Tangential-Flow Filtration-Based Large-Scale Purification Method. In: Vainio S (ed) Cell-Secreted Vesicles: Methods and Protocols. Springer US, New York, NY, pp 45\u201355"},{"key":"10718_CR131","doi-asserted-by":"publisher","first-page":"11211","DOI":"10.2147\/IJN.S548986","volume":"20","author":"S Yuan","year":"2025","unstructured":"Yuan S, Jin M, Zhang Y et al (2025) Extracellular Vesicles in Skin: Biological Function and Therapeutic Potential. Int J Nanomed 20:11211\u201311233. https:\/\/doi.org\/10.2147\/IJN.S548986","journal-title":"Int J Nanomed"},{"key":"10718_CR133","doi-asserted-by":"publisher","first-page":"4213","DOI":"10.1007\/s13277-015-4105-8","volume":"37","author":"W Zhang","year":"2016","unstructured":"Zhang W, Peng P, Kuang Y et al (2016) Characterization of exosomes derived from ovarian cancer cells and normal ovarian epithelial cells by nanoparticle tracking analysis. Tumor Biol 37:4213\u20134221. https:\/\/doi.org\/10.1007\/s13277-015-4105-8","journal-title":"Tumor Biol"},{"key":"10718_CR132","doi-asserted-by":"publisher","first-page":"9753","DOI":"10.2147\/IJN.S527475","volume":"20","author":"J Zhang","year":"2025","unstructured":"Zhang J, Kuang J, Gong S et al (2025) Inhibition of Ferroptosis by Adipose Stem Cell-Derived Apoptotic Vesicles Enhances Angiogenesis and Accelerates Diabetic Wound Healing. Int J Nanomed 20:9753\u20139770. https:\/\/doi.org\/10.2147\/IJN.S527475","journal-title":"Int J Nanomed"},{"key":"10718_CR134","doi-asserted-by":"publisher","first-page":"1029671","DOI":"10.3389\/fcell.2023.1029671","volume":"11","author":"H Zhao","year":"2023","unstructured":"Zhao H, Li Z, Wang Y et al (2023) Bioengineered MSC-derived exosomes in skin wound repair and regeneration. Front Cell Dev Biol 11:1029671. https:\/\/doi.org\/10.3389\/fcell.2023.1029671","journal-title":"Front Cell Dev Biol"},{"key":"10718_CR135","doi-asserted-by":"publisher","first-page":"260","DOI":"10.1186\/s12964-023-01251-9","volume":"21","author":"X Zou","year":"2023","unstructured":"Zou X, Lei Q, Luo X et al (2023) Advances in biological functions and applications of apoptotic vesicles. Cell Commun Signal 21:260. https:\/\/doi.org\/10.1186\/s12964-023-01251-9","journal-title":"Cell Commun Signal"}],"container-title":["Marine Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10126-026-10718-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10126-026-10718-y","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10126-026-10718-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T18:02:21Z","timestamp":1793296941000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10126-026-10718-y"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":135,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10718"],"URL":"https:\/\/doi.org\/10.1007\/s10126-026-10718-y","relation":{},"ISSN":["1436-2228","1436-2236"],"issn-type":[{"value":"1436-2228","type":"print"},{"value":"1436-2236","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10]]},"assertion":[{"value":"6 March 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"7 October 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 October 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","label":"Competing Interest","group":{"name":"EthicsHeading","label":"Declarations"}}],"article-number":"169"}}