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By analyzing RNA-Seq and microarray datasets obtained from databanks, this study aims to identify possible basic mechanisms, related to the brain, involved in altering the\u00a0gene expression under VitB12\u00a0deficiency mimicking\u00a0conditions. The\u00a0database inquiry returned datasets generated from distinctly heterogeneous experimental sets and considering the\u00a0quality and relevance requirements, two datasets from mouse and one from rat models were selected. The analyses of individual datasets highlighted a change in ribosomal gene expression in VitB12\u00a0deficiency\u00a0mimicking conditions within each system. Specifically, a divergent regulation was observed depending on the animal model: mice showed a down regulation of the ribosomal gene expression, while rats an upregulation. Interestingly, <jats:italic>E2f1<\/jats:italic> was significantly upregulated under VitB12\u00a0deficiency\u00a0mimicking conditions in the animal models, with a greater upregulation in rats. The rat model\u00a0also revealed\u00a0putative E2F1 Transcription Factor Binding Sites (TFBSs) in the promoter of the differently regulated genes involved in ribosomal gene expression. This suggested the possibility that E2F1, being greater expressed in rats, could activate the ribosomal genes having E2F1 TFBSs, thus giving a plausible explication to the divergent regulation observed in animal models. Despite the great diversity of the experimental sets used to generate the datasets considered, a common alteration of the ribosomes\u00a0exists, thereby indicating a possible basic and conserved response to VitB12 deficiency. Moreover, these findings could provide new insights on E2F1 and its association with CNS homeostasis and VitB12 deficiency.<\/jats:p>\n                <jats:p><jats:bold>Graphical Abstract<\/jats:bold><\/jats:p>","DOI":"10.1007\/s10142-023-00969-6","type":"journal-article","created":{"date-parts":[[2023,1,23]],"date-time":"2023-01-23T00:02:17Z","timestamp":1674432137000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Altered vitamin B12 metabolism in the central nervous system is associated with the modification of ribosomal gene expression: new insights from comparative RNA dataset analysis"],"prefix":"10.1007","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6546-9377","authenticated-orcid":false,"given":"Aimee Rachel","family":"Mathew","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3082-6359","authenticated-orcid":false,"given":"Virve","family":"Cavallucci","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1287-9601","authenticated-orcid":false,"given":"Marco","family":"Fidaleo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2023,1,23]]},"reference":[{"issue":"1","key":"969_CR1","doi-asserted-by":"publisher","first-page":"54","DOI":"10.3945\/an.111.001370","volume":"3","author":"LH Allen","year":"2012","unstructured":"Allen LH (2012) Vitamin B-12. 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