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In this study we aimed to identify antimicrobial resistance (AMR) genes and virulence determinants in two pan-drug resistant isolate \u201cBacteria_11\u201d and \u201cBacteria_27\u201d using whole genome sequencing. <jats:italic>Proteus mirabilis<\/jats:italic> \u201cBacteria_11\u201d and \u201cBacteria_27\u201d were isolated from two different hospitalized patients in Egypt. Antimicrobial susceptibility determined using Vitek 2 system, then whole genome sequencing (WGS) using MinION nanopore sequencing was done. Antimicrobial resistant genes and virulence determinants were identified using ResFinder, CADR AMR database, Abricate tool and VF analyzer were used respectively. Multiple sequence alignment was performed using MAFFT and FastTree, respectively. All genes were present within bacterial chromosome and no plasmid was detected. \u201cBacteria_11\u201d and \u201cBacteria_27\u201d had sizes of approximately 4,128,657 bp and 4,120,646 bp respectively, with GC content of 39.15% and 39.09%. \u201cBacteria_11\u201d and \u201cBacteria_27\u201d harbored 43 and 42 antimicrobial resistance genes respectively with different resistance mechanisms, and up to 55 and 59 virulence genes respectively. Different resistance mechanisms were identified: antibiotic inactivation, antibiotic efflux, antibiotic target replacement, and antibiotic target change. We identified several genes associated with aminoglycoside resistance, sulfonamide resistance. trimethoprim resistance tetracycline resistance proteins. Also, those responsible for chloramphenicol resistance. For beta-lactam resistance, only bla<jats:italic>VEB<\/jats:italic> and bla<jats:italic>CMY-2<\/jats:italic> genes were detected. Genome analysis revealed several virulence factors contribution in isolates pathogenicity and bacterial adaptation. As well as numerous typical secretion systems (TSSs) were present in the two isolates, including T6SS and T3SS. Whole genome sequencing of both isolates identify their genetic context of antimicrobial resistant genes and virulence determinants. This genomic analysis offers detailed representation of resistant mechanisms. Also, it clarifies <jats:italic>P. mirabilis<\/jats:italic> ability to acquire resistance and highlights the emergence of extensive drug resistant (XDR) and pan-drug resistant (PDR) strains. This may help in choosing the most appropriate antibiotic treatment and limiting broad spectrum antibiotic use.<\/jats:p>","DOI":"10.1007\/s10142-024-01419-7","type":"journal-article","created":{"date-parts":[[2024,9,3]],"date-time":"2024-09-03T03:45:23Z","timestamp":1725335123000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Genomic profiling of pan-drug resistant proteus mirabilis Isolates reveals antimicrobial resistance and virulence gene landscape"],"prefix":"10.1007","volume":"24","author":[{"given":"Sarah","family":"Soliman","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salah","family":"Abdalla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amal","family":"Zedan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shymaa","family":"Enany","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,9,3]]},"reference":[{"key":"1419_CR1","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1590\/S0037-86822011000400008","volume":"44","author":"AG Abreu","year":"2011","unstructured":"Abreu AG, Marques SG, Monteiro-Neto V, Luz RM, de Carvalho A, Gon\u00e7alves G (2011) Nosocomial infection and characterization of extended-spectrum \u03b2-lactamases-producing Enterobacteriaceae in Northeast Brazil. 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