{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T22:17:26Z","timestamp":1790201846059,"version":"4.1.0"},"reference-count":106,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,1,30]],"date-time":"2025-01-30T00:00:00Z","timestamp":1738195200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2025,1,30]],"date-time":"2025-01-30T00:00:00Z","timestamp":1738195200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100003339","name":"Consejo Superior de Investigaciones Cientificas","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003339","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Funct Integr Genomics"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Fish disease outbreaks caused by bacterial burdens are responsible for decreasing productivity in aquaculture. Unraveling the molecular mechanisms activated in the gonads after infections is pivotal for enhancing husbandry techniques in fish farms, ensuring disease management, and selecting the most resilience phenotype. The present study, with an important commercial species the European sea bass (\n                    <jats:italic>Dicentrarchus labrax<\/jats:italic>\n                    ), an important commercial species in Europe, examined changes in the miRNome and transcriptome 48\u00a0h after an intraperitoneal infection with\n                    <jats:italic>Vibrio anguillarum<\/jats:italic>\n                    . The findings indicate that following infection, testes exhibited more pronounced alterations in both the miRNome and transcriptome. Specifically, males showed approximately 26% more differentially expressed genes in testicular genes compared to females (2,624 vs. 101 DEGs). Additionally, four miRNAs (miR-183-5p, miR-191-3p, miR-451-5p, and miR-724-5p) were significantly expressed post-infection in males, while none were identified in females. Interestingly, upon deep analysis of sexual dimorphic gene modules, a larger number of miRNAs were identified in infected females targeting genes related to the immune system compared to infected males. These results suggest that fish ovaries demonstrate greater resilience in response to infections by suppressing genes related to the immune system through a post-transcriptional mechanism performed by miRNAs. In contrast, testes activate genes related to the immune system and repress genes related to cellular processes to cope with the infection. In particular, the crosstalk between the miRNome and transcriptome in infected males revealed a pivotal gene, namely, insulin-like growth factor binding protein (\n                    <jats:italic>igfbp<\/jats:italic>\n                    ), acting as a gene network hub in which miR-192-3p was connected. The current study elucidated the need to comprehend the basic immune regulatory responses associated with miRNAs and gene regulation networks that depend on fish sex. The data reveal the importance of considering sex as a factor in interpreting the immune system in fish to generate efficient protocols to prevent outbreaks in fish farms.\n                  <\/jats:p>","DOI":"10.1007\/s10142-025-01537-w","type":"journal-article","created":{"date-parts":[[2025,1,30]],"date-time":"2025-01-30T01:08:24Z","timestamp":1738199304000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Gonadal miRNomes and transcriptomes in infected fish reveal sexually dimorphic patterns of the immune response"],"prefix":"10.1007","volume":"25","author":[{"given":"Tosca A.","family":"van Gelderen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pinky","family":"Debnath","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Joly","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edgar","family":"Bertomeu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neil","family":"Duncan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dolors","family":"Furones","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laia","family":"Ribas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,1,30]]},"reference":[{"key":"1537_CR1","doi-asserted-by":"publisher","unstructured":"Alvi SM, Zayed Y, Malik R, Peng C (2021) The emerging role of microRNAs in fish ovary: a mini review. Gen Comp Endocrinol 311. https:\/\/doi.org\/10.1016\/J.YGCEN.2021.113850","DOI":"10.1016\/J.YGCEN.2021.113850"},{"key":"1537_CR2","doi-asserted-by":"publisher","first-page":"350","DOI":"10.1038\/nature02871","volume":"431","author":"V Ambros","year":"2004","unstructured":"Ambros V (2004) The functions of animal microRNAs. Nature 431:350\u2013355. https:\/\/doi.org\/10.1038\/nature02871","journal-title":"Nature"},{"key":"1537_CR3","doi-asserted-by":"publisher","first-page":"646","DOI":"10.1093\/MOLBEV\/MSX316","volume":"35","author":"G Avital","year":"2018","unstructured":"Avital G, Fran\u00e7a GS, Yanai I (2018) Bimodal Evolutionary Developmental miRNA Program in Animal Embryogenesis. Mol Biol Evol 35:646\u2013654. https:\/\/doi.org\/10.1093\/MOLBEV\/MSX316","journal-title":"Mol Biol Evol"},{"key":"1537_CR4","doi-asserted-by":"publisher","first-page":"349","DOI":"10.1038\/hdy.1948.21","volume":"2","author":"AJ Bateman","year":"1948","unstructured":"Bateman AJ (1948) Intra-sexual selection in Drosophila. Heredity (Edinb) 2:349\u2013368. https:\/\/doi.org\/10.1038\/hdy.1948.21","journal-title":"Heredity (Edinb)"},{"key":"1537_CR5","doi-asserted-by":"publisher","first-page":"44","DOI":"10.1186\/s12862-017-0885-3","volume":"17","author":"A Beemelmanns","year":"2017","unstructured":"Beemelmanns A, Roth O (2017) Grandparental immune priming in the pipefish Syngnathus typhle. BMC Evol Biol 17:44. https:\/\/doi.org\/10.1186\/s12862-017-0885-3","journal-title":"BMC Evol Biol"},{"key":"1537_CR6","doi-asserted-by":"publisher","first-page":"269","DOI":"10.1111\/raq.12474","volume":"13","author":"RA Bhat","year":"2021","unstructured":"Bhat RA, Priyam M, Foysal MJ, Gupta SK, Sundaray JK (2021) Role of sex-biased miRNAs in teleosts\u2013 a review. Rev Aquac 13:269\u2013281. https:\/\/doi.org\/10.1111\/raq.12474","journal-title":"Rev Aquac"},{"key":"1537_CR7","doi-asserted-by":"publisher","unstructured":"Bir J, Rojo-Bartolom\u00e9 I, Lekube X, Diaz de Cerio O, Ortiz-Zarragoitia M, Cancio I (2023) High production of transfer RNAs identifies the presence of developing oocytes in ovaries and intersex testes of teleost fish. Mar Environ Res 186. https:\/\/doi.org\/10.1016\/j.marenvres.2023.105907","DOI":"10.1016\/j.marenvres.2023.105907"},{"key":"1537_CR8","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1159\/000341378","volume":"6","author":"TT Bizuayehu","year":"2012","unstructured":"Bizuayehu TT, Babiak J, Norberg B, Fernandes JMO, Johansen SD, Babiak I (2012) Sex-biased miRNA expression in Atlantic Halibut (Hippoglossus hippoglossus) Brain and Gonads. Sex Dev 6:257\u2013266. https:\/\/doi.org\/10.1159\/000341378","journal-title":"Sex Dev"},{"key":"1537_CR9","doi-asserted-by":"publisher","first-page":"110989","DOI":"10.1016\/j.mce.2020.110989","volume":"518","author":"JP Breves","year":"2020","unstructured":"Breves JP, Springer-Miller RH, Chenoweth DA, Paskavitz AL, Chang AYH, Regish AM et al (2020) Cortisol regulates insulin-like growth-factor binding protein (igfbp) gene expression in Atlantic salmon parr. Mol Cell Endocrinol 518:110989. https:\/\/doi.org\/10.1016\/j.mce.2020.110989","journal-title":"Mol Cell Endocrinol"},{"key":"1537_CR10","doi-asserted-by":"publisher","unstructured":"Caballero-Huertas M, Salazar M, Ribas L (2024) Sex is a crucial factor in the immune response: an ichthyological perspective. Rev Fish Sci Aquacult 1\u201321. https:\/\/doi.org\/10.1080\/23308249.2024.2390965","DOI":"10.1080\/23308249.2024.2390965"},{"key":"1537_CR11","doi-asserted-by":"publisher","first-page":"1816","DOI":"10.1111\/RAQ.12545","volume":"13","author":"M Caballero-Huertas","year":"2021","unstructured":"Caballero-Huertas M, Soto M, Ribas L (2021) Reviewing Pseudoloma neurophilia infections in the popular zebrafish model. Rev Aquac 13:1816\u20131827. https:\/\/doi.org\/10.1111\/RAQ.12545","journal-title":"Rev Aquac"},{"key":"1537_CR12","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/1471-2105-10-421","volume":"10","author":"C Camacho","year":"2009","unstructured":"Camacho C, Coulouris G, Avagyan V, Ma N, Papadopoulos J, Bealer K et al (2009) BLAST+: Architecture and applications. BMC Bioinformatics 10:1\u20139. https:\/\/doi.org\/10.1186\/1471-2105-10-421","journal-title":"BMC Bioinformatics"},{"key":"1537_CR13","doi-asserted-by":"publisher","first-page":"350","DOI":"10.1016\/J.FSI.2019.05.048","volume":"91","author":"Y Cao","year":"2019","unstructured":"Cao Y, Wang D, Li S, Zhao J, Xu L, Liu H et al (2019) A transcriptome analysis focusing on splenic immune-related mciroRNAs of rainbow trout upon Aeromonas salmonicida subsp. salmonicida infection. Fish Shellfish Immunol 91:350\u2013357. https:\/\/doi.org\/10.1016\/J.FSI.2019.05.048","journal-title":"Fish Shellfish Immunol"},{"key":"1537_CR14","doi-asserted-by":"publisher","first-page":"235","DOI":"10.1186\/s12915-021-01163-5","volume":"19","author":"E Cardona","year":"2021","unstructured":"Cardona E, Guyomar C, Desvignes T, Montfort J, Guendouz S, Postlethwait JH et al (2021) Circulating miRNA repertoire as a biomarker of metabolic and reproductive states in rainbow trout. BMC Biol 19:235. https:\/\/doi.org\/10.1186\/s12915-021-01163-5","journal-title":"BMC Biol"},{"key":"1537_CR15","doi-asserted-by":"publisher","first-page":"42","DOI":"10.1016\/j.ygcen.2015.06.019","volume":"221","author":"M Carrillo","year":"2015","unstructured":"Carrillo M, Espigares F, Felip A, Escobar S, Mol\u00e9s G, Rodr\u00edguez R et al (2015) Updating control of puberty in male European sea bass: a holistic approach. Gen Comp Endocrinol 221:42\u201353. https:\/\/doi.org\/10.1016\/j.ygcen.2015.06.019","journal-title":"Gen Comp Endocrinol"},{"key":"1537_CR16","doi-asserted-by":"publisher","first-page":"828","DOI":"10.1007\/S12026-017-8919-X","volume":"65","author":"LM Chapman","year":"2017","unstructured":"Chapman LM, Ture SK, Field DJ, Morrell CN (2017) miR-451 limits CD4\u2009+\u2009T cell proliferative responses to infection in mice. Immunol Res 65:828\u2013840. https:\/\/doi.org\/10.1007\/S12026-017-8919-X","journal-title":"Immunol Res"},{"key":"1537_CR201","doi-asserted-by":"crossref","unstructured":"Chen J, Chen D, Li J, Liu Y, Gu X, Teng X (2021) Cadmium-induced oxidative stress and immunosuppression mediated mitochondrial apoptosis via JNK-FoxO3a-PUMA pathway in common carp (Cyprinus carpio L.) gills. Aquatic toxicology 233:105775","DOI":"10.1016\/j.aquatox.2021.105775"},{"key":"1537_CR17","doi-asserted-by":"publisher","unstructured":"Chen Y, Chen Y, Shi C, Huang Z, Zhang Y, Li S et al (2018) SOAPnuke: a MapReduce acceleration-supported software for integrated quality control and preprocessing of high-throughput sequencing data. Gigascience 7. https:\/\/doi.org\/10.1093\/gigascience\/gix120","DOI":"10.1093\/gigascience\/gix120"},{"key":"1537_CR75","unstructured":"R Core team (2023) R: A language and environment for statistical computing. Available at: https:\/\/www.r-project.org\/"},{"key":"1537_CR18","doi-asserted-by":"publisher","DOI":"10.5281\/zenodo.7682609","author":"G Cs\u00e1rdi","year":"2023","unstructured":"Cs\u00e1rdi G, Nepusz T, M\u00fcller K, Horv\u00e1t S, Traag V, Zanini F et al (2023) Igraph for R: R interface of the igraph library for graph theory and network analysis. Zenobo. https:\/\/doi.org\/10.5281\/zenodo.7682609","journal-title":"Zenobo"},{"key":"1537_CR19","doi-asserted-by":"publisher","first-page":"3913","DOI":"10.1038\/s41598-019-40361-8","volume":"9","author":"T Desvignes","year":"2019","unstructured":"Desvignes T, Batzel P, Sydes J, Eames BF, Postlethwait JH (2019) miRNA analysis with Prost! Reveals evolutionary conservation of organ-enriched expression and post-transcriptional modifications in three-spined stickleback and zebrafish. Sci Rep 9:3913. https:\/\/doi.org\/10.1038\/s41598-019-40361-8","journal-title":"Sci Rep"},{"key":"1537_CR20","first-page":"25","volume":"2","author":"aR Dey","year":"2010","unstructured":"Dey aR, Begum N, Paul SC, Noor M, Islam KM (2010) Prevalence and pathology of blood ptotozoa in pigeons reared at Mymensingh district, Bangladesh. Int J BioResearch 2:25\u201329","journal-title":"Int J BioResearch"},{"key":"1537_CR21","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/J.CBD.2018.05.002","volume":"28","author":"X Du","year":"2018","unstructured":"Du X, Liu X, Zhang K, Liu Y, Cheng J, Zhang Q (2018) Discovery and functional characterization of microRNAs and their potential roles for gonadal development in spotted knifejaw, Oplegnathus punctatus. Comp Biochem Physiol Part D Genomics Proteom 28:1\u20138. https:\/\/doi.org\/10.1016\/J.CBD.2018.05.002","journal-title":"Comp Biochem Physiol Part D Genomics Proteom"},{"key":"1537_CR22","doi-asserted-by":"publisher","first-page":"3439","DOI":"10.1093\/BIOINFORMATICS\/BTI525","volume":"21","author":"S Durinck","year":"2005","unstructured":"Durinck S, Moreau Y, Kasprzyk A, Davis S, De Moor B, Brazma A et al (2005) BioMart and Bioconductor: a powerful link between biological databases and microarray data analysis. Bioinformatics 21:3439\u20133440. https:\/\/doi.org\/10.1093\/BIOINFORMATICS\/BTI525","journal-title":"Bioinformatics"},{"key":"1537_CR23","doi-asserted-by":"publisher","unstructured":"Durinck S, Spellman PT, Birney E, Huber W (2009) Mapping identifiers for the integration of genomic datasets with the R\/Bioconductor package biomaRt. Nat Protoc 48(4):1184\u20131191. https:\/\/doi.org\/10.1038\/nprot.2009.97","DOI":"10.1038\/nprot.2009.97"},{"key":"1537_CR24","doi-asserted-by":"publisher","first-page":"152","DOI":"10.1016\/j.molimm.2017.11.015","volume":"93","author":"K Eslamloo","year":"2018","unstructured":"Eslamloo K, Inkpen SM, Rise ML, Andreassen R (2018) Discovery of microRNAs associated with the antiviral immune response of Atlantic Cod macrophages. Mol Immunol 93:152\u2013161. https:\/\/doi.org\/10.1016\/j.molimm.2017.11.015","journal-title":"Mol Immunol"},{"key":"1537_CR25","doi-asserted-by":"publisher","first-page":"742","DOI":"10.4161\/rna.20018","volume":"9","author":"A Eulalio","year":"2012","unstructured":"Eulalio A, Schulte L, Vogel J (2012) The mammalian microRNA response to bacterial infections. RNA Biol 9:742\u2013750. https:\/\/doi.org\/10.4161\/rna.20018","journal-title":"RNA Biol"},{"key":"1537_CR26","doi-asserted-by":"publisher","unstructured":"FAO (2022) The state of World fisheries and Aquaculture 2022. https:\/\/doi.org\/10.4060\/cc0461en","DOI":"10.4060\/cc0461en"},{"key":"1537_CR27","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1111\/j.1469-7998.1974.tb03150.x","volume":"174","author":"ASD Farmer","year":"1974","unstructured":"Farmer ASD (1974) Reproduction in Nephrops norvegicus (Decapoda: Nephropidae). J Zool 174:161\u2013183. https:\/\/doi.org\/10.1111\/j.1469-7998.1974.tb03150.x","journal-title":"J Zool"},{"key":"1537_CR28","doi-asserted-by":"publisher","first-page":"824","DOI":"10.1210\/er.2001-0033","volume":"23","author":"SM Firth","year":"2002","unstructured":"Firth SM, Baxter RC (2002) Cellular actions of the insulin-like growth factor binding proteins. Endocr Rev 23:824\u2013854. https:\/\/doi.org\/10.1210\/er.2001-0033","journal-title":"Endocr Rev"},{"key":"1537_CR29","doi-asserted-by":"publisher","first-page":"643","DOI":"10.1111\/J.1365-2761.2011.01279.X","volume":"34","author":"I Frans","year":"2011","unstructured":"Frans I, Michiels CW, Bossier P, Willems KA, Lievens B, Rediers H (2011) Vibrio anguillarum as a fish pathogen: virulence factors, diagnosis and prevention. J Fish Dis 34:643\u2013661. https:\/\/doi.org\/10.1111\/J.1365-2761.2011.01279.X","journal-title":"J Fish Dis"},{"key":"1537_CR30","volume-title":"Statistics (international student edition)","author":"D Freedman","year":"2007","unstructured":"Freedman D, Pisani R, Purves R (2007) Statistics (international student edition). W. W. Norton & Company, New York"},{"key":"1537_CR31","doi-asserted-by":"publisher","first-page":"550","DOI":"10.1007\/S10126-019-09903-Z","volume":"21","author":"C Gao","year":"2019","unstructured":"Gao C, Cai X, Fu Q, Yang N, Song L, Su B et al (2019a) Dynamics of MiRNA Transcriptome in Turbot (Scophthalmus maximus L.) Intestine following Vibrio anguillarum infection. Mar Biotechnol (NY) 21:550\u2013564. https:\/\/doi.org\/10.1007\/S10126-019-09903-Z","journal-title":"Mar Biotechnol (NY)"},{"key":"1537_CR32","doi-asserted-by":"publisher","first-page":"333","DOI":"10.1016\/J.FSI.2019.01.018","volume":"87","author":"C Gao","year":"2019","unstructured":"Gao C, Fu Q, Yang N, Song L, Tan F, Zhu J et al (2019b) Identification and expression profiling analysis of microRNAs in Nile tilapia (Oreochromis niloticus) in response to Streptococcus agalactiae infection. Fish Shellfish Immunol 87:333\u2013345. https:\/\/doi.org\/10.1016\/J.FSI.2019.01.018","journal-title":"Fish Shellfish Immunol"},{"key":"1537_CR34","doi-asserted-by":"publisher","first-page":"2811","DOI":"10.1093\/BIOINFORMATICS\/BTU393","volume":"30","author":"Z Gu","year":"2014","unstructured":"Gu Z, Gu L, Eils R, Schlesner M, Brors B (2014) Circlize implements and enhances circular visualization in R. Bioinformatics 30:2811\u20132812. https:\/\/doi.org\/10.1093\/BIOINFORMATICS\/BTU393","journal-title":"Bioinformatics"},{"key":"1537_CR33","doi-asserted-by":"publisher","first-page":"3321","DOI":"10.1096\/FJ.201601263R","volume":"31","author":"Y Gu","year":"2017","unstructured":"Gu Y, Ampofo E, Menger MD, Laschke MW (2017) miR-191 suppresses angiogenesis by activation of NF-kB signaling. FASEB J 31:3321\u20133333. https:\/\/doi.org\/10.1096\/FJ.201601263R","journal-title":"FASEB J"},{"key":"1537_CR35","doi-asserted-by":"publisher","first-page":"303","DOI":"10.14800\/rd.303","volume":"1","author":"I Hairul-Islam","year":"2014","unstructured":"Hairul-Islam I, Saravanan S, Sekar D, Karikalan K, Senthilkumar P, Chandrika R et al (2014) Identification of microRNAs from Atlantic salmon macrophages upon Aeromonas salmonicida infection. RNA Dis 1:303. https:\/\/doi.org\/10.14800\/rd.303","journal-title":"RNA Dis"},{"key":"1537_CR36","doi-asserted-by":"publisher","first-page":"e3045","DOI":"10.1038\/cddis.2017.440","volume":"8","author":"R Hamam","year":"2017","unstructured":"Hamam R, Hamam D, Alsaleh KA, Kassem M, Zaher W, Alfayez M et al (2017) Circulating microRNAs in breast cancer: novel diagnostic and prognostic biomarkers. Cell Death Dis 8:e3045\u2013e3045. https:\/\/doi.org\/10.1038\/cddis.2017.440","journal-title":"Cell Death Dis"},{"key":"1537_CR37","doi-asserted-by":"publisher","first-page":"312","DOI":"10.1016\/J.CBD.2019.03.010","volume":"30","author":"P He","year":"2019","unstructured":"He P, Wei P, Chen X, Lin Y, Peng J (2019) Identification and characterization of microRNAs in the gonad of Trachinotus ovatus using Solexa sequencing. Comp Biochem Physiol Part D Genomics Proteom 30:312\u2013320. https:\/\/doi.org\/10.1016\/J.CBD.2019.03.010","journal-title":"Comp Biochem Physiol Part D Genomics Proteom"},{"key":"1537_CR38","doi-asserted-by":"publisher","first-page":"585","DOI":"10.1111\/RAQ.12188","volume":"10","author":"ME Hickey","year":"2018","unstructured":"Hickey ME, Lee JL (2018) A comprehensive review of Vibrio (Listonella) anguillarum: ecology, pathology and prevention. Rev Aquac 10:585\u2013610. https:\/\/doi.org\/10.1111\/RAQ.12188","journal-title":"Rev Aquac"},{"key":"1537_CR39","doi-asserted-by":"publisher","first-page":"201","DOI":"10.1111\/j.1574-6968.2006.00618.x","volume":"269","author":"G-E Hong","year":"2007","unstructured":"Hong G-E, Kim D-G, Bae J-Y, Ahn S-H, Bai SC, Kong I-S (2007) Species-specific PCR detection of the fish pathogen, Vibrio anguillarum, using the amiB gene, which encodes N-acetylmuramoyl-l-alanine amidase. FEMS Microbiol Lett 269:201\u2013206. https:\/\/doi.org\/10.1111\/j.1574-6968.2006.00618.x","journal-title":"FEMS Microbiol Lett"},{"key":"1537_CR40","doi-asserted-by":"publisher","first-page":"1839","DOI":"10.1038\/s41598-024-52220-2","volume":"14","author":"B-L Huang","year":"2024","unstructured":"Huang B-L, Wei L-F, Lin Y-W, Huang L-S, Qu Q-Q, Li X-H et al (2024) Serum IGFBP-1 as a promising diagnostic and prognostic biomarker for colorectal cancer. Sci Rep 14:1839. https:\/\/doi.org\/10.1038\/s41598-024-52220-2","journal-title":"Sci Rep"},{"key":"1537_CR41","doi-asserted-by":"publisher","first-page":"108","DOI":"10.1016\/J.CANLET.2018.11.035","volume":"443","author":"K Ichiyama","year":"2019","unstructured":"Ichiyama K, Dong C (2019) The role of miR-183 cluster in immunity. Cancer Lett 443:108\u2013114. https:\/\/doi.org\/10.1016\/J.CANLET.2018.11.035","journal-title":"Cancer Lett"},{"key":"1537_CR42","doi-asserted-by":"publisher","first-page":"1284","DOI":"10.1016\/J.IMMUNI.2016.05.015","volume":"44","author":"K Ichiyama","year":"2016","unstructured":"Ichiyama K, Gonzalez-Martin A, Kim BS, Jin HY, Jin W, Xu W et al (2016) The microRNA-183-96-182 cluster promotes T helper 17 cell pathogenicity by negatively regulating transcription factor Foxo1 expression. Immunity 44:1284. https:\/\/doi.org\/10.1016\/J.IMMUNI.2016.05.015","journal-title":"Immunity"},{"key":"1537_CR43","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1002\/aah.10045","volume":"31","author":"MY Ina-Salwany","year":"2019","unstructured":"Ina-Salwany MY, Al\u2010saari N, Mohamad A, Mursidi F, Mohd\u2010Aris A, Amal MNA et al (2019) Vibriosis in Fish: a review on Disease Development and Prevention. J Aquat Anim Health 31:3\u201322. https:\/\/doi.org\/10.1002\/aah.10045","journal-title":"J Aquat Anim Health"},{"key":"1537_CR44","doi-asserted-by":"publisher","first-page":"262","DOI":"10.1016\/j.taap.2012.08.007","volume":"264","author":"MJ Jenny","year":"2012","unstructured":"Jenny MJ, Aluru N, Hahn ME (2012) Effects of short-term exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin on microRNA expression in zebrafish embryos. Toxicol Appl Pharmacol 264:262\u2013273. https:\/\/doi.org\/10.1016\/j.taap.2012.08.007","journal-title":"Toxicol Appl Pharmacol"},{"key":"1537_CR45","doi-asserted-by":"publisher","first-page":"680","DOI":"10.1016\/J.FSI.2019.06.058","volume":"92","author":"C Ji","year":"2019","unstructured":"Ji C, Guo X, Dong X, Ren J, Zu Y, Li W et al (2019) Notch1a can widely mediate innate immune responses in zebrafish larvae infected with Vibrio parahaemolyticus. Fish Shellfish Immunol 92:680\u2013689. https:\/\/doi.org\/10.1016\/J.FSI.2019.06.058","journal-title":"Fish Shellfish Immunol"},{"key":"1537_CR46","doi-asserted-by":"publisher","unstructured":"Jin Y, Sun F, Yang A, Yu X, Li Y, Liang S et al (2023) Insulin-like growth factor binding protein-1 and insulin in polycystic ovary syndrome: a systematic review and meta-analysis. Front Endocrinol (Lausanne) 14. https:\/\/doi.org\/10.3389\/fendo.2023.1279717","DOI":"10.3389\/fendo.2023.1279717"},{"key":"1537_CR47","doi-asserted-by":"publisher","unstructured":"Jing J, Wu J, Liu W, Xiong S, Ma W, Zhang J et al (2014) Sex-biased miRNAs in gonad and their potential roles for testis development in yellow catfish. PLoS ONE 9. https:\/\/doi.org\/10.1371\/journal.pone.0107946","DOI":"10.1371\/journal.pone.0107946"},{"key":"1537_CR48","doi-asserted-by":"publisher","unstructured":"Kim D, Paggi JM, Park C, Bennett C, Salzberg SL (2019) Graph-based genome alignment and genotyping with HISAT2 and HISAT-genotype. Nat Biotechnol 378(37):907\u2013915. https:\/\/doi.org\/10.1038\/s41587-019-0201-4","DOI":"10.1038\/s41587-019-0201-4"},{"key":"1537_CR49","doi-asserted-by":"publisher","first-page":"1155","DOI":"10.1080\/09291016.2018.1557838","volume":"51","author":"S Kumari","year":"2020","unstructured":"Kumari S, Singh R, Saikumar G (2020) Epidemiological study of porcine sapelovirus infection in pigs at Bareilly area of Uttar Pradesh, India. Biol Rhythm Res 51:1155\u20131165. https:\/\/doi.org\/10.1080\/09291016.2018.1557838","journal-title":"Biol Rhythm Res"},{"key":"1537_CR50","doi-asserted-by":"publisher","first-page":"559","DOI":"10.1186\/1471-2105-9-559","volume":"9","author":"P Langfelder","year":"2008","unstructured":"Langfelder P, Horvath S (2008) WGCNA: an R package for weighted correlation network analysis. BMC Bioinformatics 9:559. https:\/\/doi.org\/10.1186\/1471-2105-9-559","journal-title":"BMC Bioinformatics"},{"key":"1537_CR51","doi-asserted-by":"publisher","first-page":"1","DOI":"10.18637\/jss.v046.i11","volume":"46","author":"P Langfelder","year":"2012","unstructured":"Langfelder P, Horvath S (2012) Fast R functions for robust correlations and hierarchical clustering. J Stat Softw 46:1\u201317. https:\/\/doi.org\/10.18637\/jss.v046.i11","journal-title":"J Stat Softw"},{"key":"1537_CR52","doi-asserted-by":"publisher","first-page":"1690","DOI":"10.1007\/s00125-021-05427-1","volume":"64","author":"AC Lay","year":"2021","unstructured":"Lay AC, Hale LJ, Stowell-Connolly H, Pope RJP, Nair V, Ju W et al (2021) IGFBP-1 expression is reduced in human type 2 diabetic glomeruli and modulates \u03b21-integrin\/FAK signalling in human podocytes. Diabetologia 64:1690\u20131702. https:\/\/doi.org\/10.1007\/s00125-021-05427-1","journal-title":"Diabetologia"},{"key":"1537_CR53","doi-asserted-by":"publisher","first-page":"1768","DOI":"10.1002\/JCB.24843","volume":"115","author":"G Li","year":"2014","unstructured":"Li G, Zhao Y, Wen L, Liu Z, Yan F, Gao C (2014) Identification and characterization of microRNAs in the spleen of common carp immune organ. J Cell Biochem 115:1768\u20131778. https:\/\/doi.org\/10.1002\/JCB.24843","journal-title":"J Cell Biochem"},{"key":"1537_CR54","doi-asserted-by":"publisher","first-page":"6438","DOI":"10.3390\/ijms25126438","volume":"25","author":"X Li","year":"2024","unstructured":"Li X, Lin Y, Li W, Cheng Y, Zhang J, Qiu J et al (2024) Comparative analysis of mRNA, microRNA of Transcriptome, and proteomics on CIK cells responses to GCRV and Aeromonas hydrophila. Int J Mol Sci 25:6438. https:\/\/doi.org\/10.3390\/ijms25126438","journal-title":"Int J Mol Sci"},{"key":"1537_CR55","doi-asserted-by":"publisher","first-page":"923","DOI":"10.1093\/BIOINFORMATICS\/BTT656","volume":"30","author":"Y Liao","year":"2014","unstructured":"Liao Y, Smyth GK, Shi W (2014) featureCounts: an efficient general purpose program for assigning sequence reads to genomic features. Bioinformatics 30:923\u2013930. https:\/\/doi.org\/10.1093\/BIOINFORMATICS\/BTT656","journal-title":"Bioinformatics"},{"key":"1537_CR56","doi-asserted-by":"publisher","first-page":"1540","DOI":"10.1126\/science.1080372","volume":"299","author":"LP Lim","year":"2003","unstructured":"Lim LP, Glasner ME, Yekta S, Burge CB, Bartel DP (2003) Vertebrate microRNA genes. Sci (80-) 299:1540. https:\/\/doi.org\/10.1126\/science.1080372","journal-title":"Sci (80-)"},{"key":"1537_CR57","doi-asserted-by":"publisher","first-page":"898","DOI":"10.1016\/j.fsi.2020.08.045","volume":"106","author":"TD Liyanage","year":"2020","unstructured":"Liyanage TD, Nikapitiya C, Lee J, De Zoysa M (2020) Molecular insight into regulation of miRNAs in the spleen of zebrafish (Danio rerio) upon pathogenic Streptococcus parauberis infection. Fish Shellfish Immunol 106:898\u2013909. https:\/\/doi.org\/10.1016\/j.fsi.2020.08.045","journal-title":"Fish Shellfish Immunol"},{"key":"1537_CR58","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/S13059-014-0550-8","volume":"15","author":"MI Love","year":"2014","unstructured":"Love MI, Huber W, Anders S (2014) Moderated estimation of Fold change and dispersion for RNA-seq data with DESeq2. Genome Biol 15:1\u201321. https:\/\/doi.org\/10.1186\/S13059-014-0550-8","journal-title":"Genome Biol"},{"key":"1537_CR59","doi-asserted-by":"publisher","first-page":"23532","DOI":"10.1074\/JBC.M116.741264","volume":"291","author":"EA Lykken","year":"2016","unstructured":"Lykken EA, Li QJ (2016) The MicroRNA miR-191 supports T cell survival following common \u03b3 Chain Signaling. J Biol Chem 291:23532. https:\/\/doi.org\/10.1074\/JBC.M116.741264","journal-title":"J Biol Chem"},{"key":"1537_CR60","doi-asserted-by":"publisher","unstructured":"MacKenzie S, Ribas L, Pilarczyk M, Capdevila DM, Kadri S, Huntingford FA (2009) Screening for coping style increases the power of gene expression studies. PLoS ONE 4. https:\/\/doi.org\/10.1371\/journal.pone.0005314","DOI":"10.1371\/journal.pone.0005314"},{"key":"1537_CR61","doi-asserted-by":"publisher","first-page":"565","DOI":"10.1016\/S0140-6736(20)31561-0","volume":"396","author":"F Mauvais-Jarvis","year":"2020","unstructured":"Mauvais-Jarvis F, Bairey Merz N, Barnes PJ, Brinton RD, Carrero JJ, DeMeo DL et al (2020) Sex and gender: modifiers of health, disease, and medicine. Lancet (London England) 396:565\u2013582. https:\/\/doi.org\/10.1016\/S0140-6736(20)31561-0","journal-title":"Lancet (London England)"},{"key":"1537_CR62","doi-asserted-by":"publisher","first-page":"326","DOI":"10.1016\/0012-1606(75)90403-0","volume":"44","author":"A Mazabraud","year":"1975","unstructured":"Mazabraud A, Wegnez M, Denis H (1975) Biochemical research on oogenesis. RNA accumulation in the oocytes of teleosts. Dev Biol 44:326\u2013332. https:\/\/doi.org\/10.1016\/0012-1606(75)90403-0","journal-title":"Dev Biol"},{"key":"1537_CR63","doi-asserted-by":"publisher","first-page":"407","DOI":"10.4103\/ACA.ACA_94_19","volume":"22","author":"P Mishra","year":"2019","unstructured":"Mishra P, Singh U, Pandey CM, Mishra P, Pandey G (2019) Application of Student\u2019s t-test, analysis of Variance, and Covariance. Ann Card Anaesth 22:407. https:\/\/doi.org\/10.4103\/ACA.ACA_94_19","journal-title":"Ann Card Anaesth"},{"key":"1537_CR64","doi-asserted-by":"publisher","first-page":"2015","DOI":"10.1126\/science.1074196","volume":"297","author":"SL Moore","year":"2002","unstructured":"Moore SL, Wilson K (2002) Parasites as a viability cost of sexual selection in natural populations of mammals. Sci (80-) 297:2015\u20132018. https:\/\/doi.org\/10.1126\/science.1074196","journal-title":"Sci (80-)"},{"key":"1537_CR65","doi-asserted-by":"publisher","first-page":"1089","DOI":"10.1111\/TBED.13482","volume":"67","author":"A Muniesa","year":"2020","unstructured":"Muniesa A, Basurco B, Aguilera C, Furones D, Revert\u00e9 C, Sanjuan-Vilaplana A et al (2020) Mapping the knowledge of the main diseases affecting sea bass and sea bream in Mediterranean. Transbound Emerg Dis 67:1089\u20131100. https:\/\/doi.org\/10.1111\/TBED.13482","journal-title":"Transbound Emerg Dis"},{"key":"1537_CR66","doi-asserted-by":"publisher","first-page":"101257","DOI":"10.1016\/J.AQREP.2022.101257","volume":"25","author":"A Muniesa","year":"2022","unstructured":"Muniesa A, Furones D, Rodgers C, Basurco B (2022) An assessment of disease occurrence and mortality in marine fish farming in Spain. Aquac Rep 25:101257. https:\/\/doi.org\/10.1016\/J.AQREP.2022.101257","journal-title":"Aquac Rep"},{"key":"1537_CR67","doi-asserted-by":"publisher","first-page":"347","DOI":"10.1159\/000495472","volume":"11","author":"CK Muraleedharan","year":"2019","unstructured":"Muraleedharan CK, McClellan SA, Ekanayaka SA, Francis R, Zmejkoski A, Hazlett LD et al (2019) The miR-183\/96\/182 cluster regulates macrophage functions in response to Pseudomonas aeruginosa. J Innate Immun 11:347. https:\/\/doi.org\/10.1159\/000495472","journal-title":"J Innate Immun"},{"key":"1537_CR68","doi-asserted-by":"publisher","unstructured":"Nagpal N, Kulshreshtha R (2014) miR-191: an emerging player in disease biology. Front Genet 5. https:\/\/doi.org\/10.3389\/FGENE.2014.00099","DOI":"10.3389\/FGENE.2014.00099"},{"key":"1537_CR69","doi-asserted-by":"publisher","first-page":"145","DOI":"10.1016\/J.GDE.2007.02.004","volume":"17","author":"R Niwa","year":"2007","unstructured":"Niwa R, Slack FJ (2007) The evolution of animal microRNA function. Curr Opin Genet Dev 17:145\u2013150. https:\/\/doi.org\/10.1016\/J.GDE.2007.02.004","journal-title":"Curr Opin Genet Dev"},{"key":"1537_CR70","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1098\/rstb.2008.0148","volume":"364","author":"CL Nunn","year":"2009","unstructured":"Nunn CL, Lindenfors P, Pursall ER, Rolff J (2009) On sexual dimorphism in immune function. Philos Trans R Soc B Biol Sci 364:61\u201369. https:\/\/doi.org\/10.1098\/rstb.2008.0148","journal-title":"Philos Trans R Soc B Biol Sci"},{"key":"1537_CR200","doi-asserted-by":"publisher","unstructured":"Padr\u00f3s F, Caggiano M, Toffan A, Constenla M, Zarza C, Ciulli S (2022) Integrated management strategies for viral nervous necrosis (VNN) disease control in marine fish farming in the mediterranean. Pathogens 11(3):330. https:\/\/doi.org\/10.3390\/pathogens11030330. PMID: 35335654; PMCID: PMC8955002.","DOI":"10.3390\/pathogens11030330"},{"key":"1537_CR71","doi-asserted-by":"publisher","unstructured":"Papadaki M, Kaitetzidou E, Papadakis IE, Sfakianakis DG, Papandroulakis N, Mylonas CC et al (2022) Temperature-Biased miRNA Expression Patterns during European Sea Bass (Dicentrarchus labrax) Development. Int J Mol Sci 23;11164. https:\/\/doi.org\/10.3390\/IJMS231911164","DOI":"10.3390\/IJMS231911164"},{"key":"1537_CR72","doi-asserted-by":"publisher","first-page":"1794","DOI":"10.1182\/blood-2008-05-155812","volume":"113","author":"L Pase","year":"2009","unstructured":"Pase L, Layton JE, Kloosterman WP, Carradice D, Waterhouse PM, Lieschke GJ (2009) miR-451 regulates zebrafish erythroid maturation in vivo via its target gata2. Blood 113:1794\u20131804. https:\/\/doi.org\/10.1182\/blood-2008-05-155812","journal-title":"Blood"},{"key":"1537_CR73","doi-asserted-by":"publisher","first-page":"116864","DOI":"10.1016\/j.envpol.2021.116864","volume":"277","author":"F Pierron","year":"2021","unstructured":"Pierron F, Lorioux S, H\u00e9roin D, Daffe G, Etcheverria B, Cachot J et al (2021) Transgenerational epigenetic sex determination: Environment experienced by female fish affects offspring sex ratio. Environ Pollut 277:116864. https:\/\/doi.org\/10.1016\/j.envpol.2021.116864","journal-title":"Environ Pollut"},{"key":"1537_CR74","doi-asserted-by":"publisher","first-page":"210","DOI":"10.1016\/J.GENE.2017.12.062","volume":"646","author":"W Qiu","year":"2018","unstructured":"Qiu W, Zhu Y, Wu Y, Yuan C, Chen K, Li M (2018) Identification and expression analysis of microRNAs in medaka gonads. Gene 646:210\u2013216. https:\/\/doi.org\/10.1016\/J.GENE.2017.12.062","journal-title":"Gene"},{"key":"1537_CR76","doi-asserted-by":"publisher","first-page":"231","DOI":"10.1016\/j.cbpa.2012.08.003","volume":"163","author":"KM Reindl","year":"2012","unstructured":"Reindl KM, Sheridan MA (2012) Peripheral regulation of the growth hormone-insulin-like growth factor system in fish and other vertebrates. Comp Biochem Physiol Part Mol Integr Physiol 163:231\u2013245. https:\/\/doi.org\/10.1016\/j.cbpa.2012.08.003","journal-title":"Comp Biochem Physiol Part Mol Integr Physiol"},{"key":"1537_CR77","doi-asserted-by":"publisher","first-page":"1844","DOI":"10.1111\/j.0014-3820.2005.tb01831.x","volume":"59","author":"J Rolff","year":"2005","unstructured":"Rolff J, Armitage SAO, Coltman DW (2005) GENETIC CONSTRAINTS AND SEXUAL DIMORPHISM IN IMMUNE DEFENSE. Evol (N Y) 59:1844\u20131850. https:\/\/doi.org\/10.1111\/j.0014-3820.2005.tb01831.x","journal-title":"Evol (N Y)"},{"key":"1537_CR78","doi-asserted-by":"publisher","first-page":"5965","DOI":"10.4049\/JIMMUNOL.1201437","volume":"189","author":"CM Rosenberger","year":"2012","unstructured":"Rosenberger CM, Podyminogin RL, Navarro G, Zhao G-W, Askovich PS, Weiss MJ et al (2012) miR-451 regulates dendritic cell cytokine responses to influenza infection. J Immunol 189:5965\u20135975. https:\/\/doi.org\/10.4049\/JIMMUNOL.1201437","journal-title":"J Immunol"},{"key":"1537_CR79","doi-asserted-by":"publisher","first-page":"1611","DOI":"10.1111\/j.1365-2109.2005.01386.x","volume":"36","author":"JC Sainz-Hernandez","year":"2005","unstructured":"Sainz-Hernandez JC, Maeda-Martinez AN (2005) Sources of Vibrio bacteria in mollusc hatcheries and control methods: a case study. Aquac Res 36:1611\u20131618. https:\/\/doi.org\/10.1111\/j.1365-2109.2005.01386.x","journal-title":"Aquac Res"},{"key":"1537_CR80","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/S12915-022-01398","volume":"20","author":"N S\u00e1nchez-Baiz\u00e1n","year":"2022","unstructured":"S\u00e1nchez-Baiz\u00e1n N, Ribas L, Piferrer F (2022) Improved biomarker discovery through a plot twist in transcriptomic data analysis. BMC Biol 20:1\u201326. https:\/\/doi.org\/10.1186\/S12915-022-01398","journal-title":"BMC Biol"},{"key":"1537_CR81","doi-asserted-by":"publisher","unstructured":"Sarropoulou E, Kaitetzidou E, Papandroulakis N, Tsalafouta A, Pavlidis M (2019) Inventory of European Sea Bass (Dicentrarchus labrax) sncRNAs Vital during Early Teleost Development. Front Genet 10. https:\/\/doi.org\/10.3389\/fgene.2019.00657","DOI":"10.3389\/fgene.2019.00657"},{"key":"1537_CR82","doi-asserted-by":"publisher","first-page":"692","DOI":"10.1016\/J.FSI.2017.06.052","volume":"67","author":"JG Schmidt","year":"2017","unstructured":"Schmidt JG, Korbut R, Ohtani M, von J\u00f8rgensen L G (2017) Zebrafish (Danio rerio) as a model to visualize infection dynamics of Vibrio anguillarum following intraperitoneal injection and bath exposure. Fish Shellfish Immunol 67:692\u2013697. https:\/\/doi.org\/10.1016\/J.FSI.2017.06.052","journal-title":"Fish Shellfish Immunol"},{"key":"1537_CR83","doi-asserted-by":"publisher","first-page":"150","DOI":"10.1016\/j.ygcen.2017.08.002","volume":"252","author":"M Shimizu","year":"2017","unstructured":"Shimizu M, Dickhoff WW (2017) Circulating insulin-like growth factor binding proteins in fish: their identities and physiological regulation. Gen Comp Endocrinol 252:150\u2013161. https:\/\/doi.org\/10.1016\/j.ygcen.2017.08.002","journal-title":"Gen Comp Endocrinol"},{"key":"1537_CR84","doi-asserted-by":"publisher","unstructured":"Skaftnesmo KO, Edvardsen RB, Furmanek T, Crespo D, Andersson E, Kleppe L et al (2017) Integrative testis transcriptome analysis reveals differentially expressed miRNAs and their mRNA targets during early puberty in Atlantic salmon. BMC Genomics 18. https:\/\/doi.org\/10.1186\/S12864-017-4205-5","DOI":"10.1186\/S12864-017-4205-5"},{"key":"1537_CR85","doi-asserted-by":"publisher","first-page":"1496","DOI":"10.1111\/cmi.12159","volume":"15","author":"C Staedel","year":"2013","unstructured":"Staedel C, Darfeuille F (2013) MicroRNAs and bacterial infection. Cell Microbiol 15:1496\u20131507. https:\/\/doi.org\/10.1111\/cmi.12159","journal-title":"Cell Microbiol"},{"key":"1537_CR86","doi-asserted-by":"publisher","first-page":"468","DOI":"10.1038\/s43016-020-0127-5","volume":"1","author":"GD Stentiford","year":"2020","unstructured":"Stentiford GD, Bateman IJ, Hinchliffe SJ, Bass D, Hartnell R, Santos EM et al (2020) Sustainable aquaculture through the One Health lens. Nat Food 1:468\u2013474. https:\/\/doi.org\/10.1038\/s43016-020-0127-5","journal-title":"Nat Food"},{"key":"1537_CR87","doi-asserted-by":"publisher","unstructured":"van Gelderen TA, Montfort J, \u00c1lvarez-Dios JA, Piferrer F, Bobe J, Ribas L (2024) Identification of sex-biased miRNA markers informative of heat-past events. bioRxiv 20240124577069. https:\/\/doi.org\/10.1101\/2024.01.24.577069","DOI":"10.1101\/2024.01.24.577069"},{"key":"1537_CR88","doi-asserted-by":"publisher","first-page":"137","DOI":"10.1016\/j.tcb.2014.11.004","volume":"25","author":"JA Vidigal","year":"2015","unstructured":"Vidigal JA, Ventura A (2015) The biological functions of miRNAs: lessons from in vivo studies. Trends Cell Biol 25:137\u2013147. https:\/\/doi.org\/10.1016\/j.tcb.2014.11.004","journal-title":"Trends Cell Biol"},{"key":"1537_CR89","doi-asserted-by":"publisher","first-page":"958","DOI":"10.1111\/ele.13973","volume":"25","author":"O Vincze","year":"2022","unstructured":"Vincze O, V\u00e1g\u00e1si CI, P\u00e9nzes J, Szab\u00f3 K, Magonyi NM, Czirj\u00e1k G\u00c1 et al (2022) Sexual dimorphism in immune function and oxidative physiology across birds: the role of sexual selection. Ecol Lett 25:958\u2013970. https:\/\/doi.org\/10.1111\/ele.13973","journal-title":"Ecol Lett"},{"key":"1537_CR91","unstructured":"Wang ZC, Lu H, Zhou Q, Yu SM, Mao YL, Zhang HJ et al (2015) MiR-451 inhibits synovial fibroblasts proliferation and inflammatory cytokines secretion in rheumatoid arthritis through mediating p38MAPK signaling pathway. Int J Clin Exp Pathol 8;14562. Available at: https:\/\/pubmed.ncbi.nlm.nih.gov\/26823778\/. (Accessed February 14, 2024)"},{"key":"1537_CR90","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1016\/j.aquaculture.2017.11.020","volume":"485","author":"P Wang","year":"2018","unstructured":"Wang P, Wang L, Yang J, Luan P, Zhang X, Kuang Y et al (2018) Sex-biased miRNAs of yellow catfish (Pelteobagrus fulvidraco) and their potential role in reproductive development. Aquaculture 485:73\u201380. https:\/\/doi.org\/10.1016\/j.aquaculture.2017.11.020","journal-title":"Aquaculture"},{"key":"1537_CR92","doi-asserted-by":"publisher","unstructured":"Wang Z, Dai R, Ahmed SA (2023) MicroRNA-183\/96\/182 cluster in immunity and autoimmunity. Front Immunol 14. https:\/\/doi.org\/10.3389\/FIMMU.2023.1134634","DOI":"10.3389\/FIMMU.2023.1134634"},{"key":"1537_CR94","doi-asserted-by":"publisher","unstructured":"Wu T, Hu E, Xu S, Chen M, Guo P, Dai Z et al (2021) clusterProfiler 4.0: a universal enrichment tool for interpreting omics data. Innov (Cambridge) 2. https:\/\/doi.org\/10.1016\/J.XINN.2021.100141","DOI":"10.1016\/J.XINN.2021.100141"},{"key":"1537_CR93","doi-asserted-by":"publisher","unstructured":"Wu S, Huang J, Li Y, Lei M, Zhao L, Liu Z (2022) Integrated analysis of immune parameters, miRNA-mRNA interaction, and immune genes expression in the liver of rainbow trout following infectious hematopoietic necrosis virus infection. Front Immunol 13. https:\/\/doi.org\/10.3389\/FIMMU.2022.970321\/FULL","DOI":"10.3389\/FIMMU.2022.970321\/FULL"},{"key":"1537_CR95","doi-asserted-by":"publisher","first-page":"3580","DOI":"10.3390\/ANI13223580\/S1","volume":"13","author":"S Yilmaz","year":"2023","unstructured":"Yilmaz S, Karata\u015f S, Steinum TM, G\u00fcrkan M, Yilmaz DK, Abdel-Latif HMR (2023) Isolation, identification, and pathogenicity of Vibrio gigantis Retrieved from European Seabass (Dicentrarchus labrax) Farmed in T\u00fcrkiye. Animals 13:3580. https:\/\/doi.org\/10.3390\/ANI13223580\/S1","journal-title":"Animals"},{"key":"1537_CR96","doi-asserted-by":"publisher","first-page":"284","DOI":"10.1089\/omi.2011.0118","volume":"16","author":"G Yu","year":"2012","unstructured":"Yu G, Wang L-G, Han Y, He Q-Y (2012) clusterProfiler: an R Package for comparing Biological themes among Gene clusters. Omi J Integr Biol 16:284\u2013287. https:\/\/doi.org\/10.1089\/omi.2011.0118","journal-title":"Omi J Integr Biol"},{"key":"1537_CR97","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1101\/gad.1998711","volume":"25","author":"L Zhang","year":"2011","unstructured":"Zhang L, Flygare J, Wong P, Lim B, Lodish HF (2011) miR-191 regulates mouse erythroblast enucleation by down-regulating Riok3 and Mxi1. Genes Dev 25:119\u2013124. https:\/\/doi.org\/10.1101\/gad.1998711","journal-title":"Genes Dev"},{"key":"1537_CR99","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1016\/J.GENE.2018.03.014","volume":"658","author":"X Zhang","year":"2018","unstructured":"Zhang X, Li L, Jiang H, Ma JE, Li J, Chen J (2018) Identification and differential expression of microRNAs in testis and ovary of Amur Sturgeon (Acipenser schrenckii). Gene 658:36\u201346. https:\/\/doi.org\/10.1016\/J.GENE.2018.03.014","journal-title":"Gene"},{"key":"1537_CR98","doi-asserted-by":"publisher","first-page":"307","DOI":"10.1016\/J.FSI.2019.01.026","volume":"87","author":"QL Zhang","year":"2019","unstructured":"Zhang QL, Dong ZX, Luo ZW, Jiao YJ, Guo J, Deng XY et al (2019) MicroRNA profile of immune response in gills of zebrafish (Danio rerio) upon Staphylococcus aureus infection. Fish Shellfish Immunol 87:307\u2013314. https:\/\/doi.org\/10.1016\/J.FSI.2019.01.026","journal-title":"Fish Shellfish Immunol"},{"key":"1537_CR100","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/S12864-017-3995-9","volume":"18","author":"C Zhao","year":"2017","unstructured":"Zhao C, Zhang G, Yin S, Li Z, Wang Q, Chen S et al (2017) Integrated analysis of mRNA-seq and miRNA-seq reveals the potential roles of sex-biased miRNA-mRNA pairs in gonad tissue of dark sleeper (Odontobutis potamophila). BMC Genomics 18:1\u201315. https:\/\/doi.org\/10.1186\/S12864-017-3995-9","journal-title":"BMC Genomics"},{"key":"1537_CR101","doi-asserted-by":"publisher","first-page":"766","DOI":"10.1007\/s10126-021-10062-3","volume":"23","author":"N Zhao","year":"2021","unstructured":"Zhao N, Jia L, Li G, He X, Zhu C, Zhang B (2021) Comparative mucous miRomics in Cynoglossus semilaevis related to Vibrio harveyi Caused infection. Mar Biotechnol 23:766\u2013776. https:\/\/doi.org\/10.1007\/s10126-021-10062-3","journal-title":"Mar Biotechnol"},{"key":"1537_CR102","doi-asserted-by":"publisher","first-page":"e0266189","DOI":"10.1371\/JOURNAL.PONE.0266189","volume":"17","author":"Y Zhao","year":"2022","unstructured":"Zhao Y, Fan S, Yuan P, Li G (2022) Expression characteristics and interaction networks of microRNAs in spleen tissues of grass carp (Ctenopharyngodon idella). PLoS ONE 17:e0266189. https:\/\/doi.org\/10.1371\/JOURNAL.PONE.0266189","journal-title":"PLoS ONE"},{"key":"1537_CR103","doi-asserted-by":"publisher","first-page":"109312","DOI":"10.1016\/J.FSI.2023.109312","volume":"144","author":"Y Zhao","year":"2024","unstructured":"Zhao Y, Gu J, Wu R, Liu B, Dong P, Yu G et al (2024) Characteristics of conserved microRNAome and their evolutionary adaptation to regulation of immune defense functions in the spleen of silver carp and bighead carp. Fish Shellfish Immunol 144:109312. https:\/\/doi.org\/10.1016\/J.FSI.2023.109312","journal-title":"Fish Shellfish Immunol"},{"key":"1537_CR104","doi-asserted-by":"publisher","unstructured":"zur Lage P, Newton FG, Jarman AP (2019) Survey of the ciliary motility machinery of Drosophila sperm and ciliated mechanosensory neurons reveals unexpected cell-type specific variations: A model for motile ciliopathies. Front Genet 10. https:\/\/doi.org\/10.3389\/fgene.2019.00024","DOI":"10.3389\/fgene.2019.00024"}],"container-title":["Functional &amp; Integrative Genomics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10142-025-01537-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10142-025-01537-w","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10142-025-01537-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T08:09:43Z","timestamp":1769155783000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10142-025-01537-w"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,30]]},"references-count":106,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["1537"],"URL":"https:\/\/doi.org\/10.1007\/s10142-025-01537-w","relation":{"has-preprint":[{"id-type":"doi","id":"10.21203\/rs.3.rs-5306100\/v1","asserted-by":"object"}]},"ISSN":["1438-793X","1438-7948"],"issn-type":[{"value":"1438-793X","type":"print"},{"value":"1438-7948","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,30]]},"assertion":[{"value":"21 October 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"10 January 2025","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"13 January 2025","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"30 January 2025","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"European sea bass experiments were performed in well-equipped fish facilities at the Institute of Agrifood Research and Technology (IRTA, la R\u00e0pita, Tarragona, Spain,\n                      \n                      ). All animal procedures included in this project were conducted in compliance with an experimental research protocol approved by the committee of ethics and animal experimentation of the IRTA and the Catalan Government (project n\u00b0 12197, file n\u00b0 FUE-2023-03496883 and ID SLNBB6432) based on Real Decreto 53\/2013 and in accordance with the guidelines of the European Union Council (2010\/63\/EC) for the use of laboratory animals. IRTA is an authorized biosecurity facility to work with pathogens (NBS2) certified by registers numbers: EA-01\/17 and IRTA-SCRT9900002 approved by Animal Protection Section (Catalan Government). Present animal research adheres to the ARRIVE (Animal Research: Reporting of In Vivo Experiments) guidelines.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"All author consent the publication of the current article.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"29"}}