{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,7]],"date-time":"2026-11-07T11:21:42Z","timestamp":1794050502314,"version":"4.8.0"},"reference-count":38,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,5,6]],"date-time":"2025-05-06T00:00:00Z","timestamp":1746489600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,5,6]],"date-time":"2025-05-06T00:00:00Z","timestamp":1746489600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100018542","name":"Natural Science Foundation of Sichuan Province","doi-asserted-by":"crossref","award":["2025ZNSFSC0209"],"award-info":[{"award-number":["2025ZNSFSC0209"]}],"id":[{"id":"10.13039\/501100018542","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["U21A20257"],"award-info":[{"award-number":["U21A20257"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Sichuan Science and Technology Programs","award":["2021ZDZX0010"],"award-info":[{"award-number":["2021ZDZX0010"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Funct Integr Genomics"],"published-print":{"date-parts":[[2025,12]]},"DOI":"10.1007\/s10142-025-01605-1","type":"journal-article","created":{"date-parts":[[2025,5,5]],"date-time":"2025-05-05T23:13:59Z","timestamp":1746486839000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Establishment of a rapid RAA-CRISPR\/Cas12a system targeting the recN gene for on-site detection of Streptococcus suis in livestock and fresh pork meat"],"prefix":"10.1007","volume":"25","author":[{"given":"Jian","family":"Yang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjing","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yulian","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changwei","family":"Lei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongning","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,5,6]]},"reference":[{"key":"1605_CR26","doi-asserted-by":"publisher","first-page":"162","DOI":"10.1186\/s12866-016-0782-8","volume":"16","author":"TBT Athey","year":"2016","unstructured":"Athey TBT, Teatero S, Lacouture S, Takamatsu D, Gottschalk M, Fittipaldi N (2016) Determining Streptococcus suis serotype from short-read whole-genome sequencing data. BMC Microbiol 16:162","journal-title":"BMC Microbiol"},{"key":"1605_CR1","first-page":"1793","volume":"22","author":"J-P Auger","year":"2016","unstructured":"Auger J-P, Meekhanon N, Okura M, Osaki M, Gottschalk M, Sekizaki T, Takamatsu D (2016) Streptococcus suis serotype 2 capsule in vivo, emerging infect. Dis 22:1793\u20131796","journal-title":"Dis"},{"key":"1605_CR32","doi-asserted-by":"publisher","first-page":"2277","DOI":"10.1128\/JCM.43.5.2277-2285.2005","volume":"43","author":"MFC Beersma","year":"2005","unstructured":"Beersma MFC, Dirven K, van Dam AP, Templeton KE, Claas ECJ, Goossens H (2005) Evaluation of 12 commercial tests and the complement fixation test for Mycoplasma pneumoniae-Specific Immunoglobulin G (IgG and IgM antibodies, with PCR used as the gold standard. J Clin Microbiol 43:2277\u20132285","journal-title":"J Clin Microbiol"},{"key":"1605_CR10","doi-asserted-by":"publisher","first-page":"3296","DOI":"10.1128\/AEM.04167-13","volume":"80","author":"L Bonifait","year":"2014","unstructured":"Bonifait L, Veillette M, L\u00e9tourneau V, Grenier D, Duchaine C, Yates MV (2014) Detection of Streptococcus suis in bioaerosols of swine confinement buildings. Appl Environ Microbiol 80:3296\u20133304","journal-title":"Appl Environ Microbiol"},{"key":"1605_CR28","first-page":"356","volume":"20","author":"M Boolchandani","year":"2019","unstructured":"Boolchandani M, D\u2019Souza AW, Dantas G (2019) Sequencing-based methods and resources to study antimicrobial resistance. Nat Rev Genet 20:356\u2013370","journal-title":"Nat Rev Genet"},{"key":"1605_CR3","first-page":"mgen000952","volume":"9","author":"J Brizuela","year":"2023","unstructured":"Brizuela J, Kajeekul R, Roodsant TJ, Riwload A, Boueroy P, Pattanapongpaibool A, Thaipadungpanit J, Jenjaroenpun P, Wongsurawat T, Batty EM, van der Putten BCL, Schultsz C, Kerdsin A (2023) Streptococcus suis outbreak caused by an emerging zoonotic strain with acquired multi-drug resistance in Thailand. Microb Genom 9:mgen000952","journal-title":"Microb Genom"},{"key":"1605_CR4","first-page":"413","volume":"30","author":"J Brizuela","year":"2024","unstructured":"Brizuela J, Roodsant TJ, Hasnoe Q, van der Putten BCL, Kozakova J, Slotved H-C, van der Linden M, de Beer-Schuurman IGA, Sadowy E, S\u00e1ez-Nieto JA, Chalker VJ, van der Ark KCH, Schultsz C (2024) Molecular epidemiology of underreported emerging zoonotic pathogen Streptococcus suis in Europe, emerging infect. Dis 30:413\u2013422","journal-title":"Dis"},{"key":"1605_CR24","doi-asserted-by":"publisher","first-page":"14846","DOI":"10.1021\/acs.analchem.0c03883","volume":"92","author":"Y Chen","year":"2020","unstructured":"Chen Y, Mei Y, Zhao X, Jiang X, Reagents-Loaded (2020) Automated assay that integrates Recombinase-Aided amplification and Cas12a nucleic acid detection for a Point-of-Care test. Anal Chem 92:14846\u201314852","journal-title":"Anal Chem"},{"key":"1605_CR34","doi-asserted-by":"publisher","first-page":"1140","DOI":"10.1080\/22221751.2020.1763857","volume":"9","author":"LA Curti","year":"2020","unstructured":"Curti LA, Pereyra-Bonnet F, Repizo GD, Fay JV, Salvatierra K, Blariza MJ, Iba\u00f1ez-Alegre D, Rinflerch AR, Miretti M, Gimenez CA (2020) CRISPR-based platform for carbapenemases and emerging viruses detection using Cas12a (Cpf1) effector nuclease, Emerg. Microbes Infect 9:1140\u20131148","journal-title":"Microbes Infect"},{"key":"1605_CR2","doi-asserted-by":"crossref","unstructured":"Dong X, Chao Y, Zhou Y, Zhou R, Zhang W, Fischetti VA, Wang X, Feng Y, Li J (2021) The global emergence of a novel Streptococcus suis clade associated with human infections. EMBO Mol Med, 13","DOI":"10.15252\/emmm.202013810"},{"key":"1605_CR25","doi-asserted-by":"publisher","first-page":"366","DOI":"10.3390\/microorganisms9020366","volume":"9","author":"M Dresen","year":"2021","unstructured":"Dresen M, Schenk J, Berhanu Weldearegay Y, V\u00f6tsch D, Baumg\u00e4rtner W, Valentin-Weigand P, Nerlich A (2021) Streptococcus suis induces expression of Cyclooxygenase-2 in Porcine lung tissue. Microorganisms 9:366","journal-title":"Microorganisms"},{"key":"1605_CR6","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1186\/s13567-018-0606-y","volume":"49","author":"A Dumesnil","year":"2018","unstructured":"Dumesnil A, Auger J-P, Roy D, V\u00f6tsch D, Willenborg M, Valentin-Weigand P, Park PW, Grenier D, Fittipaldi N, Harel J, Gottschalk M (2018) Characterization of the zinc metalloprotease of Streptococcus suis serotype 2. Vet Res 49:109","journal-title":"Vet Res"},{"key":"1605_CR15","doi-asserted-by":"publisher","first-page":"2140","DOI":"10.1099\/ijs.0.018176-0","volume":"60","author":"OO Glazunova","year":"2010","unstructured":"Glazunova OO, Raoult D, Roux V (2010) Partial RecN gene sequencing: a new tool for identification and phylogeny within the genus Streptococcus. Int J Syst Evol Microbiol 60:2140\u20132148","journal-title":"Int J Syst Evol Microbiol"},{"key":"1605_CR37","doi-asserted-by":"crossref","unstructured":"Gootenberg JS, Abudayyeh OO, Kellner MJ, Joung J, Collins JJ, Zhang F (2018) Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm. 360(6):439\u2013444","DOI":"10.1126\/science.aaq0179"},{"key":"1605_CR19","doi-asserted-by":"publisher","first-page":"114939","DOI":"10.1016\/j.bios.2022.114939","volume":"222","author":"JdD Habimana","year":"2023","unstructured":"Habimana JdD, Mukama O, Chen G, Chen M, Amissah OB, Wang L, Liu Y, Sun Y, Li AL, Deng S, Huang J, Yan X-x, Rutaganda T, Mutangana D, Wu L-P, Huang R, Li Z (2023) Harnessing enhanced CRISPR\/Cas12a trans-cleavage activity with extended reporters and reductants for early diagnosis of Helicobacter pylori, the causative agent of peptic ulcers and stomach cancer. Biosens Bioelectron 222:114939","journal-title":"Biosens Bioelectron"},{"key":"1605_CR31","doi-asserted-by":"publisher","first-page":"2433","DOI":"10.1111\/1541-4337.13153","volume":"22","author":"J Hadi","year":"2023","unstructured":"Hadi J, Rapp D, Dhawan S, Gupta SK, Gupta TB, Brightwell G (2023) Molecular detection and characterization of foodborne bacteria: recent progresses and remaining challenges. Compr Rev Food Sci F 22:2433\u20132464","journal-title":"Compr Rev Food Sci F"},{"key":"1605_CR16","doi-asserted-by":"crossref","unstructured":"Hess J, Kreitlow A, Rohn K, Hennig-Pauka I, Abdulmawjood A (2023) Rapid Diagnostic of Streptococcus suis in Necropsy Samples of Pigs by thrA-Based Loop-Mediated Isothermal Amplification Assay, Microorganisms, 11 2447","DOI":"10.3390\/microorganisms11102447"},{"key":"1605_CR38","doi-asserted-by":"publisher","first-page":"e2202034119","DOI":"10.1073\/pnas.2202034119","volume":"119","author":"M Hu","year":"2022","unstructured":"Hu M, Qiu Z, Bi Z, Tian T, Jiang Y, Zhou X (2022) Photocontrolled crRNA activation enables robust CRISPR-Cas12a diagnostics. Proc Natl Acad Sci USA 119:e2202034119","journal-title":"Proc Natl Acad Sci USA"},{"key":"1605_CR12","doi-asserted-by":"publisher","first-page":"1606","DOI":"10.1111\/jam.14591","volume":"128","author":"H Huan","year":"2020","unstructured":"Huan H, Jiang L, Tang L, Wang Y, Guo S (2020) Isolation and characterization of Streptococcus suis strains from swine in Jiangsu Province, China. J Appl Microbiol 128:1606\u20131612","journal-title":"J Appl Microbiol"},{"key":"1605_CR27","doi-asserted-by":"crossref","unstructured":"Huang W, Wang M, Hao H, Yang R, Xie J, Su J, Lin M, Cui Y, Jiang Y (2019) Genomic epidemiological investigation of a Streptococcus suis outbreak in Guangxi, China, 2016, infection, genetics and evolution. 68:249\u2013252","DOI":"10.1016\/j.meegid.2018.12.023"},{"key":"1605_CR14","doi-asserted-by":"publisher","first-page":"66","DOI":"10.1016\/j.mimet.2014.09.003","volume":"107","author":"S Ishida","year":"2014","unstructured":"Ishida S, Tien LHT, Osawa R, Tohya M, Nomoto R, Kawamura Y, Takahashi T, Kikuchi N, Kikuchi K, Sekizaki T (2014) Development of an appropriate PCR system for the reclassification of Streptococcus suis. J Microbiol Methods 107:66\u201370","journal-title":"J Microbiol Methods"},{"key":"1605_CR36","doi-asserted-by":"publisher","first-page":"1239269","DOI":"10.3389\/fcimb.2023.1239269","volume":"13","author":"S Liu","year":"2023","unstructured":"Liu S, Huang S, Li F, Sun Y, Fu J, Xiao F, Jia N, Huang X, Sun C, Zhou J, Wang Y, Qu D (2023) Rapid detection of Pseudomonas aeruginosa by recombinase polymerase amplification combined with CRISPR-Cas12a biosensing system. Front Cell Infect 13:1239269","journal-title":"Front Cell Infect"},{"key":"1605_CR20","doi-asserted-by":"publisher","first-page":"130234","DOI":"10.1016\/j.jhazmat.2022.130234","volume":"443","author":"L Ma","year":"2023","unstructured":"Ma L, Wang J, Li Y, Liao D, Zhang W, Han X, Man S (2023) A ratiometric fluorescent biosensing platform for ultrasensitive detection of Salmonella typhimurium via CRISPR\/Cas12a and silver nanoclusters. J Hazard Mater 443:130234","journal-title":"J Hazard Mater"},{"key":"1605_CR5","doi-asserted-by":"crossref","unstructured":"Murray GGR, Hossain ASMM, Miller EL, Bruchmann S, Balmer AJ, Matuszewska M, Herbert J, Hadjirin NF, Mugabi R, Li G, Ferrando ML, Fernandes de Oliveira IM, Nguyen T, Yen PLK, Phuc HD, Zaw Moe A, Su Wai T, Gottschalk M, Aragon V, Valentin-Weigand P, Heegaard PMH, Vrieling M, Thein Maw M, Bentley H, Clavijo MJ, Wells JM, Tucker AW, Weinert LA (2023) The emergence and diversification of a zoonotic pathogen from within the microbiota of intensively farmed pigs, Proc. Natl. Acad. Sci. USA, 120 e2307773120","DOI":"10.1073\/pnas.2307773120"},{"key":"1605_CR30","doi-asserted-by":"publisher","first-page":"1250265","DOI":"10.3389\/fmicb.2023.1250265","volume":"14","author":"TL Nicholson","year":"2023","unstructured":"Nicholson TL, Kalalah AA, Eppinger M (2023) Population structure and genetic diversity of Streptococcus suis isolates obtained from the united States. Front Microbiol 14:1250265","journal-title":"Front Microbiol"},{"key":"1605_CR13","doi-asserted-by":"crossref","unstructured":"Okwumabua O, Persaud JS, Reddy PG (2001) Cloning and characterization of the gene encoding the glutamate dehydrogenase of Streptococcus suis serotype 2, Clin. Diagn. Lab. Immunol. 8:251\u2013257","DOI":"10.1128\/CDLI.8.2.251-257.2001"},{"key":"1605_CR11","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1111\/j.1574-6968.2003.tb11501.x","volume":"218","author":"O Okwumabua","year":"2003","unstructured":"Okwumabua O, O\u2019Connor M, Shull E (2003) A polymerase chain reaction (PCR) assay specific for Streptococcus suis based on the gene encoding the glutamate dehydrogenase. FEMS Microbiol Lett 218:79\u201384","journal-title":"FEMS Microbiol Lett"},{"key":"1605_CR9","doi-asserted-by":"publisher","first-page":"3329","DOI":"10.1128\/JCM.01584-12","volume":"50","author":"K Wang","year":"2012","unstructured":"Wang K, Sun X, Lu C (2012) Development of rapid Serotype-Specific PCR assays for eight serotypes of Streptococcus suis. J Clin Microbiol 50:3329\u20133334","journal-title":"J Clin Microbiol"},{"key":"1605_CR29","doi-asserted-by":"publisher","first-page":"e0030923","DOI":"10.1128\/spectrum.00309-23","volume":"11","author":"C Wang","year":"2023","unstructured":"Wang C, Wang M, Chu Y, Sun R, Li J, Li X, Sun J, Liu Y, Zhou Y, Liao X (2023) Antibiotic resistance patterns and molecular characterization of Streptococcus suis isolates from swine and humans in China. Microbiol Spectr 11:e0030923","journal-title":"Microbiol Spectr"},{"key":"1605_CR8","doi-asserted-by":"publisher","first-page":"125202","DOI":"10.1016\/j.talanta.2023.125202","volume":"267","author":"L Wang","year":"2024","unstructured":"Wang L, Sun J, Zhao J, Bai J, Zhang Y, Zhu Y, Zhang W, Wang C, Langford PR, Liu S, Li G (2024a) A CRISPR-Cas12a-based platform facilitates the detection and serotyping of Streptococcus suis serotype 2. Talanta 267:125202","journal-title":"Talanta"},{"key":"1605_CR35","doi-asserted-by":"publisher","first-page":"e0384623","DOI":"10.1128\/spectrum.03846-23","volume":"12","author":"Z Wang","year":"2024","unstructured":"Wang Z, Cheng W, Dong Z, Yao X, Deng X, Chun; O (2024b) A CRISPR\/LbCas12a-based method for detection of bacterial fruit blotch pathogens in watermelon. Microbiol Spectr 12:e0384623","journal-title":"Microbiol Spectr"},{"key":"1605_CR7","first-page":"4","volume-title":"Transcriptional host responses to infection with Streptococcus suis in a Porcine Precision-Cut lung slice model","author":"YB Weldearegay","year":"2023","unstructured":"Weldearegay YB, Brogaard L, Nerlich A, Schaaf D, Heegaard PMH, Valentin-Weigand P (2023) Transcriptional host responses to infection with Streptococcus suis in a Porcine Precision-Cut lung slice model, vol 13. Between-Strain Differences Suggest Association with Virulence Potential, Pathogens, p 4"},{"key":"1605_CR17","doi-asserted-by":"publisher","first-page":"401","DOI":"10.1186\/s12951-021-01132-8","volume":"19","author":"Y Wu","year":"2021","unstructured":"Wu Y, Battalapalli D, Hakeem MJ, Selamneni V, Zhang P, Draz MS, Ruan Z (2021) Engineered CRISPR-Cas systems for the detection and control of antibiotic-resistant infections. J Nanobiotechnol 19:401","journal-title":"J Nanobiotechnol"},{"key":"1605_CR33","doi-asserted-by":"publisher","first-page":"4432","DOI":"10.3390\/foods12244432","volume":"12","author":"D Xu","year":"2023","unstructured":"Xu D, Zeng H, Wu W, Liu H, Wang J (2023) Isothermal amplification and CRISPR\/Cas12a-System-Based assay for rapid, sensitive and visual detection of Staphylococcus aureus. Foods 12:4432","journal-title":"Foods"},{"key":"1605_CR18","doi-asserted-by":"crossref","unstructured":"Zhang N, Jing X, Liu Y, Chen M, Zhu X, Jiang J, Wang H, Li X, Hao P (2020) Interfering with Retrotransposition by two types of CRISPR effectors: Cas12a and Cas13a. Cell Discov., 6","DOI":"10.1038\/s41421-020-0164-0"},{"key":"1605_CR21","doi-asserted-by":"crossref","unstructured":"Zhou C, Li W, Zhao Y, Gu K, Liao Z, Guo B, Huang Z, Yang M, Wei H, Ma P, Li C, Li H, Tang Y, Lei C, Wang H (2023a) Sensitive detection of viable salmonella bacteria based on tertiary cascade signal amplification via splintr ligase ligation-PCR amplification-CRISPR\/Cas12a cleavage. Anal Chim Acta, 1248","DOI":"10.1016\/j.aca.2023.340885"},{"key":"1605_CR22","doi-asserted-by":"crossref","unstructured":"Zhou J, Wang T, Lan Z, Yang H, Ye X, Min R, Wang Z, Huang Q, Cao J, Gao Y, Wang W, Sun X, Zhang Y (2023b) Strategy of functional nucleic acids-mediated isothermal amplification for detection of foodborne microbial contaminants: A review. Food Res Int, 173","DOI":"10.1016\/j.foodres.2023.113286"},{"key":"1605_CR23","doi-asserted-by":"crossref","unstructured":"Zhou C, Zhao Y, Guo B, Yang M, Xu Q, Lei C, Wang H (2024) Establishment of a simple, sensitive, and specific Salmonella detection method based on Recombinase-Aided amplification combined with dsDNA-Specific nucleases. Foods, p 13","DOI":"10.3390\/foods13091380"}],"container-title":["Functional & Integrative Genomics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10142-025-01605-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10142-025-01605-1","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10142-025-01605-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T08:11:48Z","timestamp":1769155908000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10142-025-01605-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5,6]]},"references-count":38,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["1605"],"URL":"https:\/\/doi.org\/10.1007\/s10142-025-01605-1","relation":{},"ISSN":["1438-793X","1438-7948"],"issn-type":[{"value":"1438-793X","type":"print"},{"value":"1438-7948","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5,6]]},"assertion":[{"value":"24 March 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"22 April 2025","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"24 April 2025","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"6 May 2025","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"99"}}