{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T02:52:32Z","timestamp":1791255152838,"version":"4.1.0"},"reference-count":18,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100004586","name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro","doi-asserted-by":"publisher","award":["SEI-260003\/019688\/2022"],"award-info":[{"award-number":["SEI-260003\/019688\/2022"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Funct Integr Genomics"],"published-print":{"date-parts":[[2026,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Sedentary chromosomal integrons (SCIs) are large integrons that harbor extensive arrays of gene cassettes and constitute major reservoirs of bacterial genetic diversity. In\n                    <jats:italic>Vibrio<\/jats:italic>\n                    , SCI cassettes are flanked by species-associated\n                    <jats:italic>attC<\/jats:italic>\n                    repeats, here referred to as\n                    <jats:italic>Vibrio<\/jats:italic>\n                    species repeats (VXRs). Although SCIs have been proposed as ancestral sources of antibiotic resistance genes (ARGs), the extent to which SCI-associated ARGs are exchanged among\n                    <jats:italic>Vibrio<\/jats:italic>\n                    species remains poorly understood. To investigate the dynamics of SCI-associated ARGs, we analyzed 37,812 genomes representing 181\n                    <jats:italic>Vibrio<\/jats:italic>\n                    species. Using VXR mapping, comparative phylogenetics, and genome screening, we identified 8,679 putative ARG cassettes distributed across 8,517 genomes from 11 species. Despite the diversity of VXRs identified in SCIs, ARG cassettes were predominantly associated with a small subset of related VXRs shared among phylogenetically distant species, suggesting horizontal transfer of ARG-associated cassettes across the genus. Comparative analyses further suggested that members of the Cholera clade may act as potential sources of SCI-associated ARG dissemination. Five major ARG families were identified within SCIs, including\n                    <jats:italic>cat<\/jats:italic>\n                    B,\n                    <jats:italic>qnr<\/jats:italic>\n                    VC,\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CARB<\/jats:sub>\n                    ,\n                    <jats:italic>dfr<\/jats:italic>\n                    A, and\n                    <jats:italic>vat<\/jats:italic>\n                    F, some of which displayed phylogenetic patterns consistent with interspecies transfer. Functional characterization of a SCI-associated\n                    <jats:italic>qnr<\/jats:italic>\n                    VC4 cassette from\n                    <jats:italic>Vibrio mimicus<\/jats:italic>\n                    confirmed its ability to confer reduced susceptibility to ciprofloxacin in a heterologous host. Together, our findings indicate that a conserved subset of VXR backbones, mainly from the Cholera clade, plays a role in ARG dissemination across\n                    <jats:italic>Vibrio<\/jats:italic>\n                    species, highlighting SCIs as active reservoirs contributing to the emergence and spread of antimicrobial resistance.\n                  <\/jats:p>","DOI":"10.1007\/s10142-026-02068-8","type":"journal-article","created":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T02:26:48Z","timestamp":1791253608000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Vibrio repeats (VXRs) play a role in horizontal dissemination of sedentary chromosomal integron-associated antibiotic resistance genes across Vibrio species"],"prefix":"10.1007","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4877-7639","authenticated-orcid":false,"given":"Sergio Mascarenhas","family":"Morgado","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5029-7389","authenticated-orcid":false,"given":"Erica Louren\u00e7o","family":"da Fonseca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7086-2042","authenticated-orcid":false,"given":"Ana Carolina Paulo","family":"Vicente","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,10,6]]},"reference":[{"issue":"9","key":"2068_CR1","doi-asserted-by":"publisher","first-page":"e1000632","DOI":"10.1371\/journal.pgen.1000632","volume":"5","author":"M Bouvier","year":"2009","unstructured":"Bouvier M, Ducos-Galand M, Loot C et al (2009) Structural features of single-stranded integron cassette attC sites and their role in strand selection. PLoS Genet 5(9):e1000632. https:\/\/doi.org\/10.1371\/journal.pgen.1000632","journal-title":"PLoS Genet"},{"issue":"6747","key":"2068_CR2","doi-asserted-by":"publisher","first-page":"eads0768","DOI":"10.1126\/science.ads0768","volume":"388","author":"B Darracq","year":"2025","unstructured":"Darracq B, Littner E, Brunie M et al (2025) Sedentary chromosomal integrons as biobanks of bacterial antiphage defense systems. Science 388(6747):eads0768. https:\/\/doi.org\/10.1126\/science.ads0768","journal-title":"Science"},{"issue":"21","key":"2068_CR3","doi-asserted-by":"publisher","first-page":"gkaf1162","DOI":"10.1093\/nar\/gkaf1162","volume":"53","author":"K Debatisse","year":"2025","unstructured":"Debatisse K, Brunie M, Darracq B et al (2025) Bacterial natural transformation drives cassette shuffling and simplifies recombination in chromosomal integrons. Nucleic Acids Res 53(21):gkaf1162. https:\/\/doi.org\/10.1093\/nar\/gkaf1162","journal-title":"Nucleic Acids Res"},{"key":"2068_CR4","doi-asserted-by":"publisher","first-page":"e1319","DOI":"10.7717\/peerj.1319","volume":"3","author":"AM Eren","year":"2015","unstructured":"Eren AM, Esen \u00d6C, Quince C et al (2015) Anvi\u2019o: an advanced analysis and visualization platform for \u2018omics data. PeerJ 3:e1319. https:\/\/doi.org\/10.7717\/peerj.1319","journal-title":"PeerJ"},{"issue":"4","key":"2068_CR5","doi-asserted-by":"publisher","first-page":"272","DOI":"10.1111\/j.1198-743X.2004.00858.x","volume":"10","author":"AC Fluit","year":"2004","unstructured":"Fluit AC, Schmitz FJ (2004) Resistance integrons and super-integrons. Clin Microbiol Infect 10(4):272\u2013288. https:\/\/doi.org\/10.1111\/j.1198-743X.2004.00858.x","journal-title":"Clin Microbiol Infect"},{"issue":"7","key":"2068_CR6","doi-asserted-by":"publisher","first-page":"1129","DOI":"10.3201\/eid1407.080132","volume":"14","author":"EL Fonseca","year":"2008","unstructured":"Fonseca EL, Dos Santos Freitas F, Vieira VV, Vicente AC (2008) New qnr gene cassettes associated with superintegron repeats in Vibrio cholerae O1. Emerg Infect Dis 14(7):1129\u20131131. https:\/\/doi.org\/10.3201\/eid1407.080132","journal-title":"Emerg Infect Dis"},{"issue":"2","key":"2068_CR7","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1128\/MMBR.00056-13","volume":"78","author":"MR Gillings","year":"2014","unstructured":"Gillings MR (2014) Integrons: past, present, and future. 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