{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T19:54:55Z","timestamp":1787169295297,"version":"build-2736575974"},"reference-count":40,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,7,9]],"date-time":"2024-07-09T00:00:00Z","timestamp":1720483200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,7,9]],"date-time":"2024-07-09T00:00:00Z","timestamp":1720483200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Tech Coloproctol"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Adipose tissue injections, a rich source of mesenchymal stem cells, have been successfully used to promote anal fistula healing. This study aimed to investigate the efficacy of adipose tissue injection in treating patients with complex and recurrent fistulas of cryptoglandular origin.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We conducted a prospective, single-center, open-label, non-randomized, interventional clinical trial from January 2020 to December 2022. We enrolled nine patients, who were evaluated after at least 12\u00a0months of follow-up. All patients had seton removal, fistula tract excision or curettage, and a mucosal flap if possible or, alternatively, an internal opening suture. We used a commercially available system to collect and process adipose tissue prior to injection. This system allowed the collection, microfragmentation, and filtration of tissue.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Selected cases included six men and three women with a median age of 42 (range 31\u201355)\u00a0years. All patients had an extended disease course period, ranging from 3 to 13 (mean 6.6)\u00a0years, and a history of multiple previous surgeries, including two to eight interventions (a mean of 4.4 per case). All fistulas were high transsphincteric, four cases horseshoe and two cases with secondary suprasphincteric or peri-elevator tract fistulas. Six cases (66%) achieved complete fistula healing at a mean follow-up of 18 (range 12\u201336)\u00a0months. Three cases (33.3%) experienced reduced secretion and decreased anal discomfort.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>In patients with complex and recurrent fistulas, such as the ones described, many from palliative treatments with setons, the adjuvant injection of adipose tissue might help achieve complete healing or improvement in a significant percentage of cases.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>ClinicalTrials<\/jats:title>\n                    <jats:p>The study protocol was prospectively registered on ClinicalTrials.gov (NCT 04750499).<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s10151-024-02963-x","type":"journal-article","created":{"date-parts":[[2024,7,9]],"date-time":"2024-07-09T03:02:15Z","timestamp":1720494135000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Efficacy of injection of autologous adipose tissue in the treatment of patients with complex and recurrent fistula-in-ano of cryptoglandular origin"],"prefix":"10.1007","volume":"28","clinical-trial-number":[{"clinical-trial-number":"nct04750499","registry":"10.18810\/clinical-trials-gov"}],"author":[{"given":"S.","family":"Guillaumes","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N. J.","family":"Hidalgo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Bachero","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Pena","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. T.","family":"Nogueira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Ardid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Pera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,7,9]]},"reference":[{"key":"2963_CR1","doi-asserted-by":"publisher","first-page":"1337","DOI":"10.1007\/s10350-005-0055-3","volume":"48","author":"MH Whiteford","year":"2005","unstructured":"Whiteford MH, Kilkenny J, Hyman N et al (2005) Practice parameters for the treatment of perianal abscess and fistula-in-ano (revised). Dis Colon Rectum 48:1337\u20131342. https:\/\/doi.org\/10.1007\/s10350-005-0055-3","journal-title":"Dis Colon Rectum"},{"key":"2963_CR2","doi-asserted-by":"publisher","first-page":"909","DOI":"10.1007\/s10151-023-02842-x","volume":"27","author":"\u00d3 Cano-Valderrama","year":"2023","unstructured":"Cano-Valderrama \u00d3, Miguel TF, Bonito AC et al (2023) Surgical treatment trends and outcomes for anal fistula: fistulotomy is still accurate and safe. Results from a nationwide observational study. Tech Coloproctol 27:909\u2013919. https:\/\/doi.org\/10.1007\/s10151-023-02842-x","journal-title":"Tech Coloproctol"},{"key":"2963_CR3","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1016\/j.ijsu.2019.08.003","volume":"69","author":"Z Mei","year":"2019","unstructured":"Mei Z, Wang Q, Zhang Y et al (2019) Risk Factors for Recurrence after anal fistula surgery: a meta-analysis. Int J Surg 69:153\u2013164. https:\/\/doi.org\/10.1016\/j.ijsu.2019.08.003","journal-title":"Int J Surg"},{"key":"2963_CR4","doi-asserted-by":"publisher","first-page":"2407","DOI":"10.1111\/codi.15771","volume":"23","author":"MAK Motamedi","year":"2021","unstructured":"Motamedi MAK, Serahati S, Rajendran L et al (2021) Long-term outcomes after seton placement for perianal fistulas with and without Crohn\u2019s disease. Colorectal Dis 23:2407\u20132415. https:\/\/doi.org\/10.1111\/codi.15771","journal-title":"Colorectal Dis"},{"key":"2963_CR5","doi-asserted-by":"publisher","first-page":"857","DOI":"10.1007\/s00384-014-2064-y","volume":"30","author":"BK Jain","year":"2015","unstructured":"Jain BK, Garg PK (2015) Palliation for refractory anal fistula\u2014an honorable respite. Int J Colorectal Dis 30:857\u2013858. https:\/\/doi.org\/10.1007\/s00384-014-2064-y","journal-title":"Int J Colorectal Dis"},{"key":"2963_CR6","doi-asserted-by":"publisher","first-page":"762","DOI":"10.1097\/DCR.0b013e318255364a","volume":"55","author":"MD Herreros","year":"2012","unstructured":"Herreros MD, Garcia-Arranz M, Guadalajara H et al (2012) Autologous expanded adipose-derived stem cells for the treatment of complex cryptoglandular perianal fistulas: a phase III randomized clinical trial (FATT 1: Fistula advanced therapy trial 1) and long-term evaluation. Dis Colon Rectum 55:762\u2013772. https:\/\/doi.org\/10.1097\/DCR.0b013e318255364a","journal-title":"Dis Colon Rectum"},{"key":"2963_CR7","doi-asserted-by":"publisher","first-page":"1281","DOI":"10.1016\/S0140-6736(16)31203-X","volume":"388","author":"J Pan\u00e9s","year":"2016","unstructured":"Pan\u00e9s J, Garc\u00eda-Olmo D, Van Assche G et al (2016) Expanded allogeneic adipose-derived mesenchymal stem cells (Cx601) for complex perianal fistulas in Crohn\u2019s disease: a phase 3 randomised, double-blind controlled trial. The Lancet 388:1281\u20131290. https:\/\/doi.org\/10.1016\/S0140-6736(16)31203-X","journal-title":"The Lancet"},{"key":"2963_CR8","doi-asserted-by":"publisher","first-page":"1334","DOI":"10.1053\/j.gastro.2017.12.020","volume":"154","author":"J Pan\u00e9s","year":"2018","unstructured":"Pan\u00e9s J, Garc\u00eda-Olmo D, Van Assche G et al (2018) Long-term efficacy and safety of stem cell therapy (Cx601) for complex perianal fistulas in patients with Crohn\u2019s disease. Gastroenterology 154:1334-1342.e4. https:\/\/doi.org\/10.1053\/j.gastro.2017.12.020","journal-title":"Gastroenterology"},{"key":"2963_CR9","doi-asserted-by":"publisher","first-page":"713","DOI":"10.1097\/DCR.0000000000002325","volume":"65","author":"D Garcia-Olmo","year":"2022","unstructured":"Garcia-Olmo D, Gilaberte I, Binek M et al (2022) Follow-up study to evaluate the long-term safety and efficacy of darvadstrocel (mesenchymal stem cell treatment) in patients with perianal fistulizing Crohn\u2019s disease: ADMIRE-CD phase 3 randomized controlled trial. Dis Colon Rectum 65:713\u2013720. https:\/\/doi.org\/10.1097\/DCR.0000000000002325","journal-title":"Dis Colon Rectum"},{"key":"2963_CR10","doi-asserted-by":"publisher","first-page":"3330","DOI":"10.3748\/wjg.v21.i11.3330","volume":"21","author":"D Garcia-Olmo","year":"2015","unstructured":"Garcia-Olmo D, Guadalajara H, Rubio-Perez I et al (2015) Recurrent anal fistulae: limited surgery supported by stem cells. World J Gastroenterol 21:3330\u20133336. https:\/\/doi.org\/10.3748\/wjg.v21.i11.3330","journal-title":"World J Gastroenterol"},{"key":"2963_CR11","doi-asserted-by":"publisher","first-page":"295","DOI":"10.1002\/sctm.19-0271","volume":"9","author":"M Garcia-Arranz","year":"2020","unstructured":"Garcia-Arranz M, Garcia-Olmo D, Herreros MD et al (2020) Autologous adipose-derived stem cells for the treatment of complex cryptoglandular perianal fistula: a randomized clinical trial with long-term follow-up. Stem Cells Transl Med 9:295\u2013301. https:\/\/doi.org\/10.1002\/sctm.19-0271","journal-title":"Stem Cells Transl Med"},{"key":"2963_CR12","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1155\/2019\/6132340","volume":"2019","author":"MD Herreros","year":"2019","unstructured":"Herreros MD, Garcia-Olmo D, Guadalajara H et al (2019) Stem cell therapy: a compassionate use program in perianal fistula. Stem Cells Int 2019:1\u20136. https:\/\/doi.org\/10.1155\/2019\/6132340","journal-title":"Stem Cells Int"},{"key":"2963_CR13","doi-asserted-by":"publisher","first-page":"443","DOI":"10.1097\/DCR.0000000000002379","volume":"66","author":"HR Dalby","year":"2023","unstructured":"Dalby HR, Dige A, Pedersen BG et al (2023) Efficacy of injection of freshly collected autologous adipose tissue into complex cryptoglandular anal fistulas. Dis Colon Rectum 66:443\u2013450. https:\/\/doi.org\/10.1097\/DCR.0000000000002379","journal-title":"Dis Colon Rectum"},{"key":"2963_CR14","doi-asserted-by":"publisher","first-page":"1445","DOI":"10.1007\/s11605-023-05719-4","volume":"27","author":"EY Huang","year":"2023","unstructured":"Huang EY, Zhao B, Llaneras J et al (2023) Autologous fat grafting: an emerging treatment option for complex anal fistulas. J Gastrointest Surg 27:1445\u20131453. https:\/\/doi.org\/10.1007\/s11605-023-05719-4","journal-title":"J Gastrointest Surg"},{"key":"2963_CR15","doi-asserted-by":"publisher","first-page":"2208","DOI":"10.1053\/j.gastro.2019.02.005","volume":"156","author":"A Dige","year":"2019","unstructured":"Dige A, Hougaard HT, Agnholt J et al (2019) Efficacy of injection of freshly collected autologous adipose tissue into perianal fistulas in patients with Crohn\u2019s disease. Gastroenterology 156:2208-2216.e1. https:\/\/doi.org\/10.1053\/j.gastro.2019.02.005","journal-title":"Gastroenterology"},{"key":"2963_CR16","doi-asserted-by":"publisher","first-page":"321","DOI":"10.1093\/ibd\/izz051","volume":"26","author":"S Laureti","year":"2020","unstructured":"Laureti S, Gionchetti P, Cappelli A et al (2020) Refractory complex Crohn\u2019s perianal fistulas: a role for autologous microfragmented adipose tissue injection. Inflamm Bowel Dis 26:321\u2013330. https:\/\/doi.org\/10.1093\/ibd\/izz051","journal-title":"Inflamm Bowel Dis"},{"key":"2963_CR17","doi-asserted-by":"publisher","first-page":"304","DOI":"10.1007\/s40778-016-0053-5","volume":"2","author":"C Tremolada","year":"2016","unstructured":"Tremolada C, Colombo V, Ventura C (2016) Adipose tissue and mesenchymal stem cells: state of the art and lipogems\u00ae technology development. Curr Stem Cell Rep 2:304\u2013312. https:\/\/doi.org\/10.1007\/s40778-016-0053-5","journal-title":"Curr Stem Cell Rep"},{"key":"2963_CR18","doi-asserted-by":"publisher","first-page":"980","DOI":"10.1097\/DCR.0000000000001416","volume":"62","author":"W Omar","year":"2019","unstructured":"Omar W, Alqasaby A, Abdelnaby M et al (2019) Drainage seton versus external anal sphincter-sparing seton after rerouting of the fistula tract in the treatment of complex anal fistula: a randomized controlled trial. Dis Colon Rectum 62:980\u2013987. https:\/\/doi.org\/10.1097\/DCR.0000000000001416","journal-title":"Dis Colon Rectum"},{"key":"2963_CR19","doi-asserted-by":"publisher","first-page":"134","DOI":"10.1002\/bjs.1800720222","volume":"72","author":"C Mann","year":"1985","unstructured":"Mann C, Clifton M (1985) Re-routing of the track for the treatment of high anal and anorectal fistulae. Br J Surg 72:134\u2013137. https:\/\/doi.org\/10.1002\/bjs.1800720222","journal-title":"Br J Surg"},{"key":"2963_CR20","doi-asserted-by":"publisher","first-page":"2063","DOI":"10.3727\/096368912X657855","volume":"22","author":"F Bianchi","year":"2013","unstructured":"Bianchi F, Maioli M, Leonardi E et al (2013) A new nonenzymatic method and device to obtain a fat tissue derivative highly enriched in pericyte-like elements by mild mechanical forces from human lipoaspirates. Cell Transplant 22:2063\u20132077. https:\/\/doi.org\/10.3727\/096368912X657855","journal-title":"Cell Transplant"},{"key":"2963_CR21","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1007\/BF02050307","volume":"36","author":"JM Jorge","year":"1993","unstructured":"Jorge JM, Wexner SD (1993) Etiology and management of fecal incontinence. Dis Colon Rectum 36:77\u201397. https:\/\/doi.org\/10.1007\/BF02050307","journal-title":"Dis Colon Rectum"},{"key":"2963_CR22","doi-asserted-by":"publisher","first-page":"107","DOI":"10.1007\/s10151-018-1755-8","volume":"22","author":"G Naldini","year":"2018","unstructured":"Naldini G, Sturiale A, Fabiani B et al (2018) Micro-fragmented adipose tissue injection for the treatment of complex anal fistula: a pilot study accessing safety and feasibility. Tech Coloproctol 22:107\u2013113. https:\/\/doi.org\/10.1007\/s10151-018-1755-8","journal-title":"Tech Coloproctol"},{"key":"2963_CR23","doi-asserted-by":"publisher","first-page":"1276","DOI":"10.1097\/DCR.0000000000001924","volume":"64","author":"S Ascanelli","year":"2021","unstructured":"Ascanelli S, Zamboni P, Campioni D et al (2021) Efficacy and safety of treatment of complex idiopathic fistula-in-ano using autologous centrifuged adipose tissue containing progenitor cells: a randomized controlled trial. Dis Colon Rectum 64:1276\u20131285. https:\/\/doi.org\/10.1097\/DCR.0000000000001924","journal-title":"Dis Colon Rectum"},{"key":"2963_CR24","first-page":"583","volume":"90","author":"U Topal","year":"2019","unstructured":"Topal U, Eray IC, Renc\u00fczo\u011fullar\u0131 A et al (2019) Short-term results of adipose-derived stem cell therapy for the treatment of complex perianal fistula A single center experience. Ann Ital Chir 90:583\u2013589","journal-title":"Ann Ital Chir"},{"key":"2963_CR25","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1007\/s10151-022-02675-0","volume":"27","author":"R Tutino","year":"2023","unstructured":"Tutino R, Di Franco S, Massani M et al (2023) Autologous adipose-derived stromal vascular fraction and platelet concentrates for the treatment of complex perianal fistulas. Tech Coloproctol 27:135\u2013143. https:\/\/doi.org\/10.1007\/s10151-022-02675-0","journal-title":"Tech Coloproctol"},{"key":"2963_CR26","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1186\/s13619-015-0020-0","volume":"4","author":"E Oberbauer","year":"2015","unstructured":"Oberbauer E, Steffenhagen C, Wurzer C et al (2015) Enzymatic and non-enzymatic isolation systems for adipose tissue-derived cells: current state of the art. Cell Regen 4:7. https:\/\/doi.org\/10.1186\/s13619-015-0020-0","journal-title":"Cell Regen"},{"key":"2963_CR27","doi-asserted-by":"publisher","DOI":"10.1186\/s13221-016-0037-3","author":"V Ceserani","year":"2016","unstructured":"Ceserani V, Ferri A, Berenzi A et al (2016) Angiogenic and anti-inflammatory properties of micro-fragmented fat tissue and its derived mesenchymal stromal cells. Vasc Cell. https:\/\/doi.org\/10.1186\/s13221-016-0037-3","journal-title":"Vasc Cell"},{"key":"2963_CR28","doi-asserted-by":"publisher","DOI":"10.3390\/genes10060474","author":"D Polancec","year":"2019","unstructured":"Polancec D, Zenic L, Hudetz D et al (2019) Immunophenotyping of a stromal vascular fraction from microfragmented lipoaspirate used in osteoarthritis cartilage treatment and its lipoaspirate counterpart. Genes (Basel). https:\/\/doi.org\/10.3390\/genes10060474","journal-title":"Genes (Basel)"},{"key":"2963_CR29","doi-asserted-by":"publisher","first-page":"1233","DOI":"10.3727\/096368914X681603","volume":"24","author":"S Carelli","year":"2015","unstructured":"Carelli S, Messaggio F, Canazza A et al (2015) Characteristics and properties of mesenchymal stem cells derived from microfragmented adipose tissue. Cell Transplant 24:1233\u20131252. https:\/\/doi.org\/10.3727\/096368914X681603","journal-title":"Cell Transplant"},{"key":"2963_CR30","doi-asserted-by":"publisher","first-page":"1138","DOI":"10.1002\/sctm.20-0497","volume":"10","author":"MH Carstens","year":"2021","unstructured":"Carstens MH, Quintana FJ, Calderwood ST et al (2021) Treatment of chronic diabetic foot ulcers with adipose-derived stromal vascular fraction cell injections: Safety and evidence of efficacy at 1 year. Stem Cells Transl Med 10:1138\u20131147. https:\/\/doi.org\/10.1002\/sctm.20-0497","journal-title":"Stem Cells Transl Med"},{"key":"2963_CR31","doi-asserted-by":"publisher","first-page":"883","DOI":"10.1007\/s10151-022-02683-0","volume":"26","author":"VG Sophie","year":"2022","unstructured":"Sophie VG, Marlene SJ, Helene HT et al (2022) Injection of freshly collected autologous adipose tissue in complicated pilonidal disease: a prospective pilot study. Tech Coloproctol 26:883\u2013891. https:\/\/doi.org\/10.1007\/s10151-022-02683-0","journal-title":"Tech Coloproctol"},{"key":"2963_CR32","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1186\/s13287-019-1328-4","volume":"10","author":"R Lonardi","year":"2019","unstructured":"Lonardi R, Leone N, Gennai S et al (2019) Autologous micro-fragmented adipose tissue for the treatment of diabetic foot minor amputations: a randomized controlled single-center clinical trial (MiFrAADiF). Stem Cell Res Ther 10:223. https:\/\/doi.org\/10.1186\/s13287-019-1328-4","journal-title":"Stem Cell Res Ther"},{"key":"2963_CR33","doi-asserted-by":"publisher","first-page":"NP546","DOI":"10.1093\/asj\/sjaa111","volume":"40","author":"M Ghiasloo","year":"2020","unstructured":"Ghiasloo M, Lobato RC, D\u00edaz JM et al (2020) Expanding clinical indications of mechanically isolated stromal vascular fraction: a systematic review. Aesthet Surg J 40:NP546\u2013NP560. https:\/\/doi.org\/10.1093\/asj\/sjaa111","journal-title":"Aesthet Surg J"},{"key":"2963_CR34","doi-asserted-by":"publisher","first-page":"112","DOI":"10.1111\/iwj.12569","volume":"14","author":"N Naderi","year":"2017","unstructured":"Naderi N, Combellack EJ, Griffin M et al (2017) The regenerative role of adipose-derived stem cells (ADSC) in plastic and reconstructive surgery. Int Wound J 14:112\u2013124. https:\/\/doi.org\/10.1111\/iwj.12569","journal-title":"Int Wound J"},{"key":"2963_CR35","doi-asserted-by":"publisher","first-page":"1137","DOI":"10.1111\/ans.17656","volume":"92","author":"J Hermann","year":"2022","unstructured":"Hermann J, Cwali\u0144ski J, Banasiewicz T, Ko\u0142odziejczak B (2022) Comparison between application of platelet rich plasma and mucosal advancement flap in patients with high transsphincteric anal fistulas: a randomized control trial. ANZ J Surg 92:1137\u20131141. https:\/\/doi.org\/10.1111\/ans.17656","journal-title":"ANZ J Surg"},{"key":"2963_CR36","doi-asserted-by":"publisher","first-page":"70","DOI":"10.1007\/s00384-023-04367-z","volume":"38","author":"Y Wang","year":"2023","unstructured":"Wang Y, Rao Q, Ma Y, Li X (2023) Platelet-rich plasma in the treatment of anal fistula: a systematic review and meta-analysis. Int J Colorectal Dis 38:70. https:\/\/doi.org\/10.1007\/s00384-023-04367-z","journal-title":"Int J Colorectal Dis"},{"key":"2963_CR37","doi-asserted-by":"publisher","first-page":"337","DOI":"10.5114\/wiitm.2014.47435","volume":"10","author":"G Cestaro","year":"2015","unstructured":"Cestaro G, De Rosa M, Massa S et al (2015) Intersphincteric anal lipofilling with micro-fragmented fat tissue for the treatment of faecal incontinence: preliminary results of three patients. Wideochirurgia I Inne Techniki Maloinwazyjne 10:337\u2013341. https:\/\/doi.org\/10.5114\/wiitm.2014.47435","journal-title":"Wideochirurgia I Inne Techniki Maloinwazyjne"},{"key":"2963_CR38","doi-asserted-by":"publisher","first-page":"144","DOI":"10.3393\/ac.2018.09.15","volume":"35","author":"H Jeong","year":"2019","unstructured":"Jeong H, Hwang SH, Kim HR et al (2019) Effectiveness of autologous fat graft in treating fecal incontinence. Ann Coloproctol 35:144\u2013151. https:\/\/doi.org\/10.3393\/ac.2018.09.15","journal-title":"Ann Coloproctol"},{"key":"2963_CR39","doi-asserted-by":"publisher","first-page":"181","DOI":"10.3390\/children7100181","volume":"7","author":"G Parente","year":"2020","unstructured":"Parente G, Pinto V, Di Salvo N et al (2020) Preliminary study on the echo-assisted intersphincteric autologous microfragmented adipose tissue injection to control fecal incontinence in children operated for anorectal malformations. Children 7:181. https:\/\/doi.org\/10.3390\/children7100181","journal-title":"Children"},{"key":"2963_CR40","doi-asserted-by":"publisher","DOI":"10.3390\/jcm12041258","author":"V Pinto","year":"2023","unstructured":"Pinto V, Pignatti M, Parente G et al (2023) Role of autologous fat grafting in the conservative treatment of fecal incontinence in children. J Clin Med. https:\/\/doi.org\/10.3390\/jcm12041258","journal-title":"J Clin Med"}],"container-title":["Techniques in Coloproctology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10151-024-02963-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10151-024-02963-x\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10151-024-02963-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,11]],"date-time":"2024-12-11T17:03:00Z","timestamp":1733936580000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10151-024-02963-x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,9]]},"references-count":40,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,12]]}},"alternative-id":["2963"],"URL":"https:\/\/doi.org\/10.1007\/s10151-024-02963-x","relation":{},"ISSN":["1123-6337","1128-045X"],"issn-type":[{"value":"1123-6337","type":"print"},{"value":"1128-045X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,9]]},"assertion":[{"value":"12 February 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"8 June 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"9 July 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare that they have no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"The Hospital Clinic Ethics Committee (reg. HCB\/2020\/0935) approved the study, which was conducted in accordance with the Declaration of Helsinki. We informed all patients about the treatment and obtained a written informed consent form.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}}],"article-number":"81"}}