{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T02:11:17Z","timestamp":1780452677346,"version":"3.54.1"},"reference-count":41,"publisher":"Springer Science and Business Media LLC","issue":"5","license":[{"start":{"date-parts":[[2021,8,20]],"date-time":"2021-08-20T00:00:00Z","timestamp":1629417600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2021,8,20]],"date-time":"2021-08-20T00:00:00Z","timestamp":1629417600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["JARO"],"published-print":{"date-parts":[[2021,10]]},"DOI":"10.1007\/s10162-021-00810-6","type":"journal-article","created":{"date-parts":[[2021,8,20]],"date-time":"2021-08-20T14:04:15Z","timestamp":1629468255000},"page":"591-599","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Identification of Cellular Voids in the Human Otic Capsule"],"prefix":"10.1007","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5538-9779","authenticated-orcid":false,"given":"Lars Juul","family":"Hansen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sune Land","family":"Bloch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mads S\u00f8lvsten","family":"S\u00f8rensen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,8,20]]},"reference":[{"key":"810_CR1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2009.080627","author":"TL Andersen","year":"2009","unstructured":"Andersen TL, Sondergaard TE, Skorzynska KE et al (2009) A physical mechanism for coupling bone resorption and formation in adult human bone. Am J Pathol. https:\/\/doi.org\/10.2353\/ajpath.2009.080627","journal-title":"Am J Pathol"},{"key":"810_CR2","doi-asserted-by":"publisher","first-page":"1101","DOI":"10.1002\/ar.22492","volume":"295","author":"SL Bloch","year":"2012","unstructured":"Bloch SL, Kristensen SL, S\u00f8rensen MS (2012) The viability of perilabyrinthine osteocytes: a quantitative study using bulk-stained undecalcified human temporal bones. Anat Rec (hoboken) 295:1101\u20131108. https:\/\/doi.org\/10.1002\/ar.22492","journal-title":"Anat Rec (hoboken)"},{"key":"810_CR3","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1016\/j.heares.2010.09.007","volume":"270","author":"SL Bloch","year":"2010","unstructured":"Bloch SL, S\u00f8rensen MS (2010) The viability and spatial distribution of osteocytes in the human labyrinthine capsule: a quantitative study using vector-based stereology. Hear Res 270:65\u201370. https:\/\/doi.org\/10.1016\/j.heares.2010.09.007","journal-title":"Hear Res"},{"key":"810_CR4","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.heares.2016.02.002","volume":"335","author":"SL Bloch","year":"2016","unstructured":"Bloch SL, S\u00f8rensen MS (2016) The role of connectivity and stochastic osteocyte behavior in the distribution of perilabyrinthine bone degeneration. A Monte Carlo based simulation study. Hear Res 335:1\u20138. https:\/\/doi.org\/10.1016\/j.heares.2016.02.002","journal-title":"Hear Res"},{"key":"810_CR5","doi-asserted-by":"publisher","first-page":"e1","DOI":"10.1097\/MAO.0000000000000218","volume":"35","author":"SL Bloch","year":"2014","unstructured":"Bloch SL, S\u00f8rensen MS (2014) Unbiased stereologic estimation of the spatial distribution of Paget\u2019s disease in the human temporal bone. Otol Neurotol 35:e1-6. https:\/\/doi.org\/10.1097\/MAO.0000000000000218","journal-title":"Otol Neurotol"},{"issue":"Suppl 3","key":"810_CR6","doi-asserted-by":"publisher","first-page":"131","DOI":"10.2215\/CJN.04151206","volume":"3","author":"B Clarke","year":"2008","unstructured":"Clarke B (2008) Normal bone anatomy and physiology. Clin J Am Soc Nephrol 3(Suppl 3):131\u2013139. https:\/\/doi.org\/10.2215\/CJN.04151206","journal-title":"Clin J Am Soc Nephrol"},{"key":"810_CR7","doi-asserted-by":"publisher","first-page":"577","DOI":"10.1136\/jcp.2007.048868","volume":"61","author":"HK Datta","year":"2008","unstructured":"Datta HK, Ng WF, Walker JA et al (2008) The cell biology of bone metabolism. J Clin Pathol 61:577\u2013587. https:\/\/doi.org\/10.1136\/jcp.2007.048868","journal-title":"J Clin Pathol"},{"key":"810_CR8","doi-asserted-by":"publisher","first-page":"369","DOI":"10.1007\/s00401-008-0410-2","volume":"117","author":"KA Dorph-Petersen","year":"2009","unstructured":"Dorph-Petersen KA, Caric D, Saghafi R et al (2009) Volume and neuron number of the lateral geniculate nucleus in schizophrenia and mood disorders. Acta Neuropathol 117:369\u2013384. https:\/\/doi.org\/10.1007\/s00401-008-0410-2","journal-title":"Acta Neuropathol"},{"key":"810_CR9","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1146\/annurev-pathol-011110-130203","volume":"6","author":"X Feng","year":"2011","unstructured":"Feng X, McDonald JM (2011) Disorders of Bone Remodeling. Annu Rev Pathol Mech Dis 6:121\u2013145. https:\/\/doi.org\/10.1146\/annurev-pathol-011110-130203","journal-title":"Annu Rev Pathol Mech Dis"},{"key":"810_CR10","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/421746","volume":"2015","author":"R Florencio-Silva","year":"2015","unstructured":"Florencio-Silva R, Sasso GRDS, Sasso-Cerri E et al (2015)  Biology of Bone Tissue: Structure, Function, and Factors That Influence Bone Cells. Biomed Res Int 2015:421746. https:\/\/doi.org\/10.1155\/2015\/421746 (Epub 2015 J)","journal-title":"Biomed Res Int"},{"key":"810_CR11","doi-asserted-by":"publisher","first-page":"771","DOI":"10.3109\/00016489.2015.1035400","volume":"135","author":"T Frisch","year":"2015","unstructured":"Frisch T, Bloch SL, S\u00f8rensen MS (2015) Prevalence, size and distribution of microdamage in the human otic capsule. Acta Otolaryngol 135:771\u2013775. https:\/\/doi.org\/10.3109\/00016489.2015.1035400","journal-title":"Acta Otolaryngol"},{"key":"810_CR12","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1159\/000124294","volume":"70","author":"T Frisch","year":"2008","unstructured":"Frisch T, Bretlau P, Sorensen MS (2008) Intravital microlesions in the human otic capsule. Detection, classification and pathogenetic significance revisited. ORL J Otorhinolaryngol Relat Spec 70:195\u2013201. https:\/\/doi.org\/10.1159\/000124294","journal-title":"ORL J Otorhinolaryngol Relat Spec"},{"key":"810_CR13","doi-asserted-by":"publisher","first-page":"749","DOI":"10.1177\/000348940111000810","volume":"110","author":"T Frisch","year":"2001","unstructured":"Frisch T, S\u00f8rensen MS, Bretlau P (2001a) Demonstration of intravital microfissures in undecalcified plastic-embedded temporal bones with the prestaining technique. Ann Otol Rhinol Laryngol 110:749\u2013757. https:\/\/doi.org\/10.1177\/000348940111000810","journal-title":"Ann Otol Rhinol Laryngol"},{"key":"810_CR14","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1007\/s004050000286","volume":"258","author":"T Frisch","year":"2001","unstructured":"Frisch T, S\u00f8rensen MS, Bretlau P (2001b) Recognition of basic fuchsin prestained microfissures of intravital origin with fluorescence microscopy: validation of a shortcut. Eur Arch Otorhinolaryngol 258:55\u201360","journal-title":"Eur Arch Otorhinolaryngol"},{"key":"810_CR15","doi-asserted-by":"publisher","unstructured":"Frost HM (1960a) Micropetrosis. J Bone Joint Surg Am 42 A:144\u2013150. https:\/\/doi.org\/10.2106\/00004623-196042010-00012","DOI":"10.2106\/00004623-196042010-00012"},{"key":"810_CR16","doi-asserted-by":"crossref","unstructured":"Frost HM (1960b) In vivo osteocyte death. J Bone Joint Surg Am 42-A:138\u2013143","DOI":"10.2106\/00004623-196042010-00011"},{"key":"810_CR17","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1111\/j.1365-2818.1986.tb02764.x","volume":"143","author":"HJG Gundersen","year":"1986","unstructured":"Gundersen HJG (1986) Stereology of arbitrary particles. J Microsc 143:3\u201345. https:\/\/doi.org\/10.1111\/j.1365-2818.1986.tb02764.x","journal-title":"J Microsc"},{"key":"810_CR18","doi-asserted-by":"publisher","first-page":"961","DOI":"10.1002\/ar.24535","volume":"304","author":"LJ Hansen","year":"2020","unstructured":"Hansen LJ, Bloch SL, Frisch T, S\u00f8rensen MS (2020) Microcrack surface density in the human otic capsule: An unbiased stereological quantification. Anat Rec 304:961\u2013967. https:\/\/doi.org\/10.1002\/ar.24535","journal-title":"Anat Rec"},{"key":"810_CR19","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1111\/j.1365-2818.1983.tb04233.x","volume":"131","author":"J Hanstede","year":"1983","unstructured":"Hanstede J, Gerrits P (1983) The effects of embedding in water-soluble plastics on the final dimensions of liver sections. J Microsc 131:79\u201386","journal-title":"J Microsc"},{"key":"810_CR20","doi-asserted-by":"publisher","first-page":"147","DOI":"10.1242\/jcs.01581","volume":"118","author":"K Holmbeck","year":"2005","unstructured":"Holmbeck K, Bianco P, Pidoux I et al (2005) The metalloproteinase MT1-MMP is required for normal development and maintenance of osteocyte processes in bone. J Cell Sci 118:147\u2013156. https:\/\/doi.org\/10.1242\/jcs.01581","journal-title":"J Cell Sci"},{"key":"810_CR21","volume-title":"Unbiased Stereology","author":"CV Howard","year":"2010","unstructured":"Howard CV, Reed MG (2010) Unbiased Stereology. QTP Publications, Colerain, Second edi"},{"key":"810_CR22","doi-asserted-by":"publisher","first-page":"203","DOI":"10.1111\/j.1365-2818.1985.tb02613.x","volume":"138","author":"V Howard","year":"1985","unstructured":"Howard V, Reid S, Baddeley A, Boyde A (1985) Unbiased estimation of particle density in the tandem scanning reflected light microscope. J Microsc 138:203\u2013212. https:\/\/doi.org\/10.1111\/j.1365-2818.1985.tb02613.x","journal-title":"J Microsc"},{"key":"810_CR23","doi-asserted-by":"publisher","first-page":"33814","DOI":"10.1074\/jbc.M607290200","volume":"281","author":"K Inoue","year":"2006","unstructured":"Inoue K, Mikuni-Takagaki Y, Oikawa K et al (2006) A crucial role for matrix metalloproteinase 2 in osteocytic canalicular formation and bone metabolism. J Biol Chem 281:33814\u201333824. https:\/\/doi.org\/10.1074\/jbc.M607290200","journal-title":"J Biol Chem"},{"key":"810_CR24","doi-asserted-by":"publisher","first-page":"264","DOI":"10.1016\/j.bone.2012.11.038","volume":"54","author":"RL Jilka","year":"2013","unstructured":"Jilka RL, Noble B, Weinstein RS (2013) Osteocyte apoptosis. Bone 54:264\u2013271. https:\/\/doi.org\/10.1016\/j.bone.2012.11.038","journal-title":"Bone"},{"key":"810_CR25","doi-asserted-by":"publisher","first-page":"1409","DOI":"10.1016\/S0021-9290(03)00123-4","volume":"36","author":"ML Knothe Tate","year":"2003","unstructured":"Knothe Tate ML (2003) \u201cWhither flows the fluid in bone?\u201d An osteocyte\u2019s perspective. J Biomech 36:1409\u20131424. https:\/\/doi.org\/10.1016\/S0021-9290(03)00123-4","journal-title":"J Biomech"},{"key":"810_CR26","doi-asserted-by":"publisher","first-page":"93","DOI":"10.3109\/00016486609119554","volume":"62","author":"D Mendoza","year":"1966","unstructured":"Mendoza D, Rius M (1966) Histology of the enchondral layer of the human otic capsule: Areas of devitalized and necrotic bone. Acta Otolaryngol 62:93\u2013100. https:\/\/doi.org\/10.3109\/00016486609119554","journal-title":"Acta Otolaryngol"},{"key":"810_CR27","doi-asserted-by":"publisher","first-page":"2170","DOI":"10.1016\/S0022-5347(01)66722-3","volume":"154","author":"KK Nielsen","year":"1995","unstructured":"Nielsen KK, Andersen CB, Kromann-Andersen B (1995) A Comparison Between the Effects of Paraffin and Plastic Embedding of the Normal and Obstructed Minipig Detrusor Muscle Using the Optical Dissector. J Urol 154:2170\u20132173. https:\/\/doi.org\/10.1016\/S0022-5347(01)66722-3","journal-title":"J Urol"},{"key":"810_CR28","doi-asserted-by":"publisher","first-page":"727","DOI":"10.1097\/MAO.0000000000000664","volume":"36","author":"MC Nielsen","year":"2015","unstructured":"Nielsen MC, Martin-Bertelsen T, Friis M et al (2015) Differential gene expression in the otic capsule and the middle ear\u2013an annotation of bone-related signaling genes. Otol Neurotol 36:727\u2013732. https:\/\/doi.org\/10.1097\/MAO.0000000000000664","journal-title":"Otol Neurotol"},{"key":"810_CR29","doi-asserted-by":"publisher","first-page":"273","DOI":"10.1016\/s8756-3282(96)00365-1","volume":"20","author":"BS Noble","year":"1997","unstructured":"Noble BS, Stevens H, Loveridge N, Reeve J (1997) Identification of apoptotic changes in osteocytes in normal and pathological human bone. Bone 20:273\u2013282. https:\/\/doi.org\/10.1016\/s8756-3282(96)00365-1","journal-title":"Bone"},{"key":"810_CR30","doi-asserted-by":"publisher","first-page":"273","DOI":"10.1002\/jcb.240550303","volume":"55","author":"AM Parfitt","year":"1994","unstructured":"Parfitt AM (1994) Osteonal and hemi-osteonal remodeling: The spatial and temporal framework for signal traffic in adult human bone. J Cell Biochem 55:273\u2013286. https:\/\/doi.org\/10.1002\/jcb.240550303","journal-title":"J Cell Biochem"},{"key":"810_CR31","doi-asserted-by":"publisher","first-page":"717","DOI":"10.1136\/jcp.20.5.717","volume":"20","author":"AI Prentice","year":"1967","unstructured":"Prentice AI (1967) Autofluorescence of bone tissues. J Clin Pathol 20:717\u2013719. https:\/\/doi.org\/10.1136\/jcp.20.5.717","journal-title":"J Clin Pathol"},{"key":"810_CR32","doi-asserted-by":"publisher","first-page":"335","DOI":"10.1007\/978-1-61779-415-5_22","volume":"816","author":"S Riahi","year":"2012","unstructured":"Riahi S, Noble B (2012) Techniques for the study of apoptosis in bone. Methods Mol Biol 816:335\u2013349. https:\/\/doi.org\/10.1007\/978-1-61779-415-5_22","journal-title":"Methods Mol Biol"},{"key":"810_CR33","doi-asserted-by":"publisher","first-page":"56","DOI":"10.1007\/BF00175673","volume":"249","author":"M S\u00f8lvsten S\u00f8rensen","year":"1992","unstructured":"S\u00f8lvsten S\u00f8rensen M, Balslev J\u00f8rgensen M, Bretlau P (1992) Drift barriers in the postcartilaginous development of the mammalian otic capsule. Eur Arch Oto-Rhino-Laryngology 249:56\u201361. https:\/\/doi.org\/10.1007\/BF00175673","journal-title":"Eur Arch Oto-Rhino-Laryngology"},{"key":"810_CR34","first-page":"1","volume":"512","author":"MS S\u00f8rensen","year":"1994","unstructured":"S\u00f8rensen MS (1994) Temporal bone dynamics, the hard way. Formation, growth, modeling, repair and quantum type bone remodeling in the otic capsule. Acta Otolaryngol Suppl 512:1\u201322","journal-title":"Acta Otolaryngol Suppl"},{"key":"810_CR35","doi-asserted-by":"publisher","first-page":"217","DOI":"10.3109\/00016489009122540","volume":"110","author":"MS S\u00f8rensen","year":"1990","unstructured":"S\u00f8rensen MS, Bretlau P, J\u00f8rgensen MB (1990) Quantum type bone remodeling in the otic capsule of the pig. Acta Otolaryngol 110:217\u2013223","journal-title":"Acta Otolaryngol"},{"key":"810_CR36","doi-asserted-by":"publisher","first-page":"4","DOI":"10.3109\/00016489209136839","volume":"112","author":"SS S\u00f8rensen","year":"1992","unstructured":"S\u00f8rensen SS, Bretlau P, J\u00f8rgensen MB (1992) Quantum type bone remodeling in the human otic capsule: Morphometric findings. Acta Otolaryngol 112:4\u201310. https:\/\/doi.org\/10.3109\/00016489209136839","journal-title":"Acta Otolaryngol"},{"key":"810_CR37","first-page":"335","volume":"50","author":"A Tami","year":"2002","unstructured":"Tami A, Nasser P, Verborgt O et al (2002) The role of interstitial fluid flow in the remodeling response to fatigue and disuse. Am Soc Mech Eng Bioeng Div BED 50:335\u2013336","journal-title":"Am Soc Mech Eng Bioeng Div BED"},{"key":"810_CR38","doi-asserted-by":"publisher","unstructured":"Tiede-Lewis LAM, Xie Y, Hulbert MA et al (2017) Degeneration of the osteocyte network in the C57BL\/6 mouse model of aging. Aging (Albany NY) 9:2187\u20132205. \nhttps:\/\/doi.org\/10.18632\/aging.101308","DOI":"10.18632\/aging.101308"},{"key":"810_CR39","doi-asserted-by":"publisher","first-page":"375","DOI":"10.1016\/S8756-3282(00)00236-2","volume":"26","author":"D Vashishth","year":"2000","unstructured":"Vashishth D, Verborgt O, Divine G et al (2000) Decline in osteocyte lacunar density in human cortical bone is associated with accumulation of microcracks with age. Bone 26:375\u2013380. https:\/\/doi.org\/10.1016\/S8756-3282(00)00236-2","journal-title":"Bone"},{"key":"810_CR40","doi-asserted-by":"publisher","first-page":"172","DOI":"10.1097\/01.mlg.0000150702.28451.35","volume":"115","author":"AF Zehnder","year":"2005","unstructured":"Zehnder AF, Kristiansen AG, Adams JC et al (2005) Osteoprotegerin in the inner ear may inhibit bone remodeling in the otic capsule. Laryngoscope 115:172\u2013177. https:\/\/doi.org\/10.1097\/01.mlg.0000150702.28451.35","journal-title":"Laryngoscope"},{"key":"810_CR41","doi-asserted-by":"publisher","first-page":"201","DOI":"10.1097\/01.mlg.0000191466.09210.9a","volume":"116","author":"AF Zehnder","year":"2006","unstructured":"Zehnder AF, Kristiansen AG, Adams JC et al (2006) Osteoprotegrin knockout mice demonstrate abnormal remodeling of the otic capsule and progressive hearing loss. Laryngoscope 116:201\u2013206. https:\/\/doi.org\/10.1097\/01.mlg.0000191466.09210.9a","journal-title":"Laryngoscope"}],"container-title":["Journal of the Association for Research in Otolaryngology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10162-021-00810-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10162-021-00810-6\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10162-021-00810-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,27]],"date-time":"2021-09-27T21:29:24Z","timestamp":1632778164000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10162-021-00810-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,8,20]]},"references-count":41,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2021,10]]}},"alternative-id":["810"],"URL":"https:\/\/doi.org\/10.1007\/s10162-021-00810-6","relation":{},"ISSN":["1525-3961","1438-7573"],"issn-type":[{"value":"1525-3961","type":"print"},{"value":"1438-7573","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,8,20]]},"assertion":[{"value":"24 February 2021","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"28 July 2021","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"20 August 2021","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare that they have no conflict of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of Interest"}}]}}