{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T04:03:54Z","timestamp":1790827434126,"version":"4.1.0"},"reference-count":42,"publisher":"Springer Science and Business Media LLC","issue":"3","license":[{"start":{"date-parts":[[2025,4,16]],"date-time":"2025-04-16T00:00:00Z","timestamp":1744761600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2025,4,16]],"date-time":"2025-04-16T00:00:00Z","timestamp":1744761600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["JARO"],"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Purpose<\/jats:title>\n            <jats:p>In the receptor organs of the inner ear, hair cells detect mechanical stimuli such as sounds and accelerations by deflection of their hair bundles. Myosin regulatory light chain (RLC) and non-muscle myosin II (NM2) are expressed at the apical surfaces of hair cells, and NM2 and the phosphorylation of RLC by myosin light chain kinase (MLCK) have earlier been shown to regulate the shapes of hair cells\u2019 apical surfaces in rodents. The aim of our study was to elucidate the function of myosin molecules on hair cell physiology.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>We investigated the expression of NM2 and RLC in the bullfrog\u2019s saccule by immunostaining. Using NM2 and MLCK inhibitors, we measured the stiffness, spontaneous oscillation, and resting open probability of frog hair bundles. Six to ten saccules from pleural animals were used in each experiment. In addition, we recorded auditory brainstem responses in ten mice after transtympanic injection of an MLCK inhibitor.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>We confirmed the expression of NM2A\/B and MYL9 on the apical surfaces of hair cells and of NM2A and MYL12A in hair bundles. We found that NM2 and MLCK inhibitors reduce the stiffness of hair bundles from the bullfrog's saccule. Moreover, MLCK inhibition inhibits the spontaneous oscillation of hair bundles and increases the resting open probability of transduction channels. In addition, MLCK inhibition elevates hearing thresholds in mice.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>We conclude that NM2 and the phosphorylation of RLC modulate the physiological function of hair cells and thereby help to set the normal operating conditions of hair bundles.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1007\/s10162-025-00986-1","type":"journal-article","created":{"date-parts":[[2025,4,16]],"date-time":"2025-04-16T15:51:38Z","timestamp":1744818698000},"page":"225-238","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Influence of Myosin Regulatory Light Chain and Myosin Light Chain Kinase on the Physiological Function of Inner Ear Hair Cells"],"prefix":"10.1007","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8991-0315","authenticated-orcid":false,"given":"Ryohei","family":"Oya","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwang Min","family":"Woo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian","family":"Fabella","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. G.","family":"Alonso","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paloma","family":"Bravo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. J.","family":"Hudspeth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,4,16]]},"reference":[{"key":"986_CR1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/0896-6273(94)90147-3","volume":"12","author":"AJ Hudspeth","year":"1994","unstructured":"Hudspeth AJ, Gillespie PG (1994) Pulling springs to tune transduction: adaptation by hair cells. Neuron 12:1\u20139. https:\/\/doi.org\/10.1016\/0896-6273(94)90147-3","journal-title":"Neuron"},{"key":"986_CR2","doi-asserted-by":"publisher","first-page":"6020","DOI":"10.1093\/emboj\/19.22.6020","volume":"19","author":"P Kussel-Andermann","year":"2000","unstructured":"Kussel-Andermann P, El-Amraoui A, Safieddine S, Nouaille S, Perfettini I, Lecuit M, Cossart P, Wolfrum U, Petit C (2000) Vezatin, a novel transmembrane protein, bridges myosin VIIA to the cadherin-catenins complex. EMBO J 19:6020\u20136029. https:\/\/doi.org\/10.1093\/emboj\/19.22.6020","journal-title":"EMBO J"},{"key":"986_CR3","doi-asserted-by":"publisher","first-page":"172","DOI":"10.1016\/j.conb.2015.08.006","volume":"34","author":"B Zhao","year":"2015","unstructured":"Zhao B, Muller U (2015) The elusive mechanotransduction machinery of hair cells. Curr Opin Neurobiol 34:172\u2013179. https:\/\/doi.org\/10.1016\/j.conb.2015.08.006","journal-title":"Curr Opin Neurobiol"},{"key":"986_CR4","doi-asserted-by":"publisher","first-page":"581","DOI":"10.1016\/0896-6273(93)90071-X","volume":"11","author":"PG Gillespie","year":"1993","unstructured":"Gillespie PG, Wagner MC, Hudspeth AJ (1993) Identification of a 120 Kd hair-bundle myosin located near stereociliary tips. Neuron 11:581\u2013594. https:\/\/doi.org\/10.1016\/0896-6273(93)90071-X","journal-title":"Neuron"},{"key":"986_CR5","doi-asserted-by":"publisher","first-page":"13336","DOI":"10.1073\/pnas.97.24.13336","volume":"97","author":"B Kachar","year":"2000","unstructured":"Kachar B, Parakkal M, Kurc M, Zhao Y, Gillespie PG (2000) High-resolution structure of hair-cell tip links. Proc Natl Acad Sci U S A 97:13336\u201313341. https:\/\/doi.org\/10.1073\/pnas.97.24.13336","journal-title":"Proc Natl Acad Sci U S A"},{"key":"986_CR6","doi-asserted-by":"publisher","first-page":"2066","DOI":"10.1038\/s41467-020-15936-z","volume":"11","author":"SH Li","year":"2020","unstructured":"Li SH, Mecca A, Kim J et al (2020) Myosin-VIIa is expressed in multiple isoforms and essential for tensioning the hair cell mechanotransduction complex. Nat Commun 11:2066. https:\/\/doi.org\/10.1038\/s41467-020-15936-z","journal-title":"Nat Commun"},{"key":"986_CR7","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1016\/j.cell.2009.09.010","volume":"139","author":"PG Gillespie","year":"2009","unstructured":"Gillespie PG, Muller U (2009) Mechanotransduction by hair cells: models, molecules, and mechanisms. Cell 139:33\u201344. https:\/\/doi.org\/10.1016\/j.cell.2009.09.010","journal-title":"Cell"},{"key":"986_CR8","doi-asserted-by":"publisher","first-page":"11476","DOI":"10.1073\/pnas.1104161108","volume":"108","author":"M Grati","year":"2011","unstructured":"Grati M, Kachar B (2011) Myosin VIIa and sans localization at stereocilia upper tip-link density implicates these Usher syndrome proteins in mechanotransduction. Proc Natl Acad Sci U S A 108:11476\u201311481. https:\/\/doi.org\/10.1073\/pnas.1104161108","journal-title":"Proc Natl Acad Sci U S A"},{"key":"986_CR9","doi-asserted-by":"publisher","first-page":"530","DOI":"10.1016\/j.neuron.2008.07.012","volume":"59","author":"AJ Hudspeth","year":"2008","unstructured":"Hudspeth AJ (2008) Making an effort to listen: mechanical amplification in the ear. Neuron 59:530\u2013545. https:\/\/doi.org\/10.1016\/j.neuron.2008.07.012","journal-title":"Neuron"},{"key":"986_CR10","doi-asserted-by":"publisher","unstructured":"Liu Y, Qi J, Chen X et al (2019) Critical role of spectrin in hearing development and deafness. Sci Adv 5:eaav7803. https:\/\/doi.org\/10.1126\/sciadv.aav7803","DOI":"10.1126\/sciadv.aav7803"},{"key":"986_CR11","doi-asserted-by":"publisher","first-page":"731","DOI":"10.1016\/j.cub.2013.03.039","volume":"23","author":"S Ebrahim","year":"2013","unstructured":"Ebrahim S, Fujita T, Millis BA et al (2013) NMII forms a contractile transcellular sarcomeric network to regulate apical cell junctions and tissue geometry. Curr Biol 23:731\u2013736. https:\/\/doi.org\/10.1016\/j.cub.2013.03.039","journal-title":"Curr Biol"},{"key":"986_CR12","doi-asserted-by":"publisher","first-page":"425","DOI":"10.1007\/s10162-021-00796-1","volume":"22","author":"R Oya","year":"2021","unstructured":"Oya R, Tsukamoto O, Sato T, Kato H, Matsuoka K, Oshima K, Kamakura T, Ohta Y, Imai T, Takashima S, Inohara H (2021) Phosphorylation of MYL12 by myosin light chain kinase regulates cellular shape changes in cochlear hair cells. J Assoc Res Otolaryngol 22:425\u2013441. https:\/\/doi.org\/10.1007\/s10162-021-00796-1","journal-title":"J Assoc Res Otolaryngol"},{"key":"986_CR13","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1016\/j.ydbio.2009.09.009","volume":"341","author":"JM Sawyer","year":"2010","unstructured":"Sawyer JM, Harrell JR, Shemer G, Sullivan-Brown J, Roh-Johnson M, Goldstein B (2010) Apical constriction: a cell shape change that can drive morphogenesis. Dev Biol 341:5\u201319. https:\/\/doi.org\/10.1016\/j.ydbio.2009.09.009","journal-title":"Dev Biol"},{"key":"986_CR14","doi-asserted-by":"publisher","first-page":"5191","DOI":"10.1242\/jcs.02626","volume":"118","author":"JD Hildebrand","year":"2005","unstructured":"Hildebrand JD (2005) Shroom regulates epithelial cell shape via the apical positioning of an actomyosin network. J Cell Sci 118:5191\u20135203. https:\/\/doi.org\/10.1242\/jcs.02626","journal-title":"J Cell Sci"},{"key":"986_CR15","doi-asserted-by":"publisher","first-page":"1977","DOI":"10.1242\/dev.030718","volume":"136","author":"N Yamamoto","year":"2009","unstructured":"Yamamoto N, Okano T, Ma XF, Adelstein RS, Kelley MW (2009) Myosin II regulates extension, growth and patterning in the mammalian cochlear duct. Development 136:1977\u20131986. https:\/\/doi.org\/10.1242\/dev.030718","journal-title":"Development"},{"key":"986_CR16","doi-asserted-by":"publisher","first-page":"1373","DOI":"10.1242\/dev.045138","volume":"137","author":"R Etournay","year":"2010","unstructured":"Etournay R, Lepelletier L, Boutet de Monvel J, Michel V, Cayet N, Leibovici M, Weil D, Foucher I, Hardelin JP, Petit C (2010) Cochlear outer hair cells undergo an apical circumference remodeling constrained by the hair bundle shape. Development 137:1373\u20131383. https:\/\/doi.org\/10.1242\/dev.045138","journal-title":"Development"},{"key":"986_CR17","doi-asserted-by":"publisher","first-page":"2218","DOI":"10.1253\/circj.CJ-13-0627","volume":"77","author":"O Tsukamoto","year":"2013","unstructured":"Tsukamoto O, Kitakaze M (2013) Biochemical and physiological regulation of cardiac myocyte contraction by cardiac-specific myosin light chain kinase. Circ J 77:2218\u20132225. https:\/\/doi.org\/10.1253\/circj.CJ-13-0627","journal-title":"Circ J"},{"key":"986_CR18","doi-asserted-by":"publisher","first-page":"898","DOI":"10.1016\/S0006-3495(96)79293-7","volume":"71","author":"RJ Levine","year":"1996","unstructured":"Levine RJ, Kensler RW, Yang Z, Stull JT, Sweeney HL (1996) Myosin light chain phosphorylation affects the structure of rabbit skeletal muscle thick filaments. Biophys J 71:898\u2013907. https:\/\/doi.org\/10.1016\/S0006-3495(96)79293-7","journal-title":"Biophys J"},{"key":"986_CR19","doi-asserted-by":"publisher","first-page":"e34894","DOI":"10.1371\/journal.pone.0034894","volume":"7","author":"GJ Zhu","year":"2012","unstructured":"Zhu GJ, Wang F, Chen C et al (2012) Myosin light-chain kinase is necessary for membrane homeostasis in cochlear inner hair cells. PLoS ONE 7:e34894. https:\/\/doi.org\/10.1371\/journal.pone.0034894","journal-title":"PLoS ONE"},{"key":"986_CR20","doi-asserted-by":"publisher","first-page":"905","DOI":"10.3892\/ijmm.2014.1634","volume":"33","author":"X Yang","year":"2014","unstructured":"Yang X, Wang JG, Ma DB, Ma XF, Zhu GJ, Zhou H, Yu CJ, Qian XY, Gao X (2014) Myosin light chain kinase regulates hearing in mice by influencing the F-actin cytoskeleton of outer hair cells and cochleae. Int J Mol Med 33:905\u2013912. https:\/\/doi.org\/10.3892\/ijmm.2014.1634","journal-title":"Int J Mol Med"},{"key":"986_CR21","doi-asserted-by":"publisher","first-page":"e18312","DOI":"10.7554\/eLife.18312","volume":"5","author":"CP Morgan","year":"2016","unstructured":"Morgan CP, Krey JF, Grad M, Zhao B, Fallen S, Kannan-Sundhari A, Liu XZ, Choi D, Muller U, Barr-Gillespie PG (2016) PDZD7-MYO7A complex identified in enriched stereocilia membranes. Elife 5:e18312. https:\/\/doi.org\/10.7554\/eLife.18312","journal-title":"Elife"},{"key":"986_CR22","doi-asserted-by":"publisher","first-page":"102132","DOI":"10.1016\/j.ceb.2022.102132","volume":"79","author":"ZG Moreland","year":"2022","unstructured":"Moreland ZG, Bird JE (2022) Myosin motors in sensory hair bundle assembly. Curr Opin Cell Biol 79:102132. https:\/\/doi.org\/10.1016\/j.ceb.2022.102132","journal-title":"Curr Opin Cell Biol"},{"key":"986_CR23","doi-asserted-by":"publisher","first-page":"910","DOI":"10.1021\/jm980573a","volume":"42","author":"TJ Lukas","year":"1999","unstructured":"Lukas TJ, Mirzoeva S, Slomczynska U, Watterson DM (1999) Identification of novel classes of protein kinase inhibitors using combinatorial peptide chemistry based on functional genomics knowledge. J Med Chem 42:910\u2013919. https:\/\/doi.org\/10.1021\/jm980573a","journal-title":"J Med Chem"},{"key":"986_CR24","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1053\/gast.2002.34235","volume":"123","author":"Y Zolotarevsky","year":"2002","unstructured":"Zolotarevsky Y, Hecht G, Koutsouris A, Gonzalez DE, Quan C, Tom J, Mrsny RJ, Turner JR (2002) A membrane-permeant peptide that inhibits MLC kinase restores barrier function in in vitro models of intestinal disease. Gastroenterology 123:163\u2013172. https:\/\/doi.org\/10.1053\/gast.2002.34235","journal-title":"Gastroenterology"},{"key":"986_CR25","doi-asserted-by":"publisher","unstructured":"Azimzadeh JB, Salvi JD (2017) Physiological preparation of hair cells from the sacculus of the American bullfrog (Rana catesbeiana). J Vis Exp. https:\/\/doi.org\/10.3791\/55380","DOI":"10.3791\/55380"},{"key":"986_CR26","doi-asserted-by":"publisher","first-page":"14306","DOI":"10.1073\/pnas.96.25.14306","volume":"96","author":"P Martin","year":"1999","unstructured":"Martin P, Hudspeth AJ (1999) Active hair-bundle movements can amplify a hair cell\u2019s response to oscillatory mechanical stimuli. Proc Natl Acad Sci U S A 96:14306\u201314311. https:\/\/doi.org\/10.1073\/pnas.96.25.14306","journal-title":"Proc Natl Acad Sci U S A"},{"key":"986_CR27","doi-asserted-by":"publisher","first-page":"30722","DOI":"10.1073\/pnas.2016858117","volume":"117","author":"RG Alonso","year":"2020","unstructured":"Alonso RG, Tobin M, Martin P, Hudspeth AJ (2020) Fast recovery of disrupted tip links induced by mechanical displacement of hair bundles. Proc Natl Acad Sci U S A 117:30722\u201330727. https:\/\/doi.org\/10.1073\/pnas.2016858117","journal-title":"Proc Natl Acad Sci U S A"},{"key":"986_CR28","doi-asserted-by":"publisher","first-page":"E1000","DOI":"10.1073\/pnas.1501453112","volume":"112","author":"JD Salvi","year":"2015","unstructured":"Salvi JD, Maoil\u00e9idigh DO, Fabella BA, Tobin M, Hudspeth AJ (2015) Control of a hair bundle\u2019s mechanosensory function by its mechanical load. Proc Natl Acad Sci U S A 112:E1000-1009. https:\/\/doi.org\/10.1073\/pnas.1501453112","journal-title":"Proc Natl Acad Sci U S A"},{"key":"986_CR29","doi-asserted-by":"publisher","first-page":"829","DOI":"10.1038\/s41592-022-01507-1","volume":"19","author":"D Ershov","year":"2022","unstructured":"Ershov D, Phan MS, Pylvanainen JW et al (2022) TrackMate 7: integrating state-of-the-art segmentation algorithms into tracking pipelines. Nat Methods 19:829\u2013832. https:\/\/doi.org\/10.1038\/s41592-022-01507-1","journal-title":"Nat Methods"},{"key":"986_CR30","doi-asserted-by":"publisher","first-page":"676","DOI":"10.1038\/nmeth.2019","volume":"9","author":"J Schindelin","year":"2012","unstructured":"Schindelin J, Arganda-Carreras I, Frise E et al (2012) Fiji: an open-source platform for biological-image analysis. Nat Methods 9:676\u2013682. https:\/\/doi.org\/10.1038\/nmeth.2019","journal-title":"Nat Methods"},{"key":"986_CR31","doi-asserted-by":"publisher","first-page":"942","DOI":"10.1523\/JNEUROSCI.03-05-00942.1983","volume":"3","author":"DP Corey","year":"1983","unstructured":"Corey DP, Hudspeth AJ (1983) Analysis of the microphonic potential of the bullfrogs sacculus. J Neurosci 3:942\u2013961. https:\/\/doi.org\/10.1523\/JNEUROSCI.03-05-00942.1983","journal-title":"J Neurosci"},{"key":"986_CR32","doi-asserted-by":"publisher","first-page":"962","DOI":"10.1523\/JNEUROSCI.03-05-00962.1983","volume":"3","author":"DP Corey","year":"1983","unstructured":"Corey DP, Hudspeth AJ (1983) Kinetics of the receptor current in bullfrog saccular hair-cells. J Neurosci 3:962\u2013976. https:\/\/doi.org\/10.1523\/JNEUROSCI.03-05-00962.1983","journal-title":"J Neurosci"},{"key":"986_CR33","doi-asserted-by":"publisher","first-page":"249","DOI":"10.1002\/jmor.1051760302","volume":"176","author":"M Huangfu","year":"1983","unstructured":"Huangfu M, Saunders JC (1983) Auditory development in the mouse-structural maturation of the middle-ear. J Morphol 176:249\u2013259. https:\/\/doi.org\/10.1002\/jmor.1051760302","journal-title":"J Morphol"},{"key":"986_CR34","doi-asserted-by":"publisher","unstructured":"Parham K, Sun XM, Kim DO (2001) Noninvasive assessment of auditory function in mice: auditory brainstem response and distortion product otoacoustic emissions from behavior to molecular biology. In: handbook of mouse auditory research. Willot JF, editor. CRC Press, Boca Raton, pp. 37\u201358. https:\/\/doi.org\/10.1201\/97814200387","DOI":"10.1201\/97814200387"},{"key":"986_CR35","doi-asserted-by":"publisher","first-page":"4533","DOI":"10.1523\/JNEUROSCI.23-11-04533.2003","volume":"23","author":"P Martin","year":"2003","unstructured":"Martin P, Bozovic D, Choe Y, Hudspeth AJ (2003) Spontaneous oscillation by hair bundles of the bullfrog\u2019s sacculus. J Neurosci 23:4533\u20134548. https:\/\/doi.org\/10.1523\/JNEUROSCI.23-11-04533.2003","journal-title":"J Neurosci"},{"key":"986_CR36","doi-asserted-by":"publisher","first-page":"603","DOI":"10.1016\/j.bpj.2011.06.027","volume":"101","author":"Y Roongthumskul","year":"2011","unstructured":"Roongthumskul Y, Fredrickson-Hemsing L, Kao A, Bozovic D (2011) Multiple-timescale dynamics underlying spontaneous oscillations of saccular hair bundles. Biophys J 101:603\u2013610. https:\/\/doi.org\/10.1016\/j.bpj.2011.06.027","journal-title":"Biophys J"},{"key":"986_CR37","doi-asserted-by":"publisher","first-page":"480","DOI":"10.1016\/s0959-4388(97)80026-8","volume":"7","author":"AJ Hudspeth","year":"1997","unstructured":"Hudspeth AJ (1997) Mechanical amplification of stimuli by hair cells. Curr Opin Neurobiol 7:480\u2013486. https:\/\/doi.org\/10.1016\/s0959-4388(97)80026-8","journal-title":"Curr Opin Neurobiol"},{"key":"986_CR38","doi-asserted-by":"publisher","first-page":"2487","DOI":"10.1523\/Jneurosci.22-07-02487.2002","volume":"22","author":"JL Cyr","year":"2002","unstructured":"Cyr JL, Dumont RA, Gillespie PG (2002) Myosin-1c interacts with hair-cell receptors through its calmodulin-binding IQ domains. J Neurosci 22:2487\u20132495. https:\/\/doi.org\/10.1523\/Jneurosci.22-07-02487.2002","journal-title":"J Neurosci"},{"key":"986_CR39","doi-asserted-by":"publisher","first-page":"12390","DOI":"10.1073\/pnas.1409459111","volume":"111","author":"JE Bird","year":"2014","unstructured":"Bird JE, Takagi Y, Billington N, Strub MP, Sellers JR, Friedman TB (2014) Chaperone-enhanced purification of unconventional myosin 15, a molecular motor specialized for stereocilia protein trafficking. Proc Natl Acad Sci U S A 111:12390\u201312395. https:\/\/doi.org\/10.1073\/pnas.1409459111","journal-title":"Proc Natl Acad Sci U S A"},{"key":"986_CR40","doi-asserted-by":"publisher","first-page":"17587","DOI":"10.1074\/jbc.M114.599365","volume":"290","author":"T Sakai","year":"2015","unstructured":"Sakai T, Jung HS, Sato O, Yamada MD, You DJ, Ikebe R, Ikebe M (2015) Structure and regulation of the movement of human myosin VIIA. J Biol Chem 290:17587\u201317598. https:\/\/doi.org\/10.1074\/jbc.M114.599365","journal-title":"J Biol Chem"},{"key":"986_CR41","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1038\/nsmb.2923","volume":"22","author":"Q Lu","year":"2015","unstructured":"Lu Q, Li JC, Ye F, Zhang MJ (2015) Structure of myosin-1c tail bound to calmodulin provides insights into calcium-mediated conformational coupling. Nat Struct Mol Biol 22:81\u201388. https:\/\/doi.org\/10.1038\/nsmb.2923","journal-title":"Nat Struct Mol Biol"},{"key":"986_CR42","doi-asserted-by":"publisher","first-page":"C239","DOI":"10.1152\/ajpcell.1998.275.1.C239","volume":"275","author":"T Krarup","year":"1998","unstructured":"Krarup T, Jakobsen LD, Jensen BS, Hoffmann EK (1998) Na+-K+-2Cl- cotransport in Ehrlich cells: regulation by protein phosphatases and kinases. Am J Physiol-Cell Ph 275:C239\u2013C250. https:\/\/doi.org\/10.1152\/ajpcell.1998.275.1.C239","journal-title":"Am J Physiol-Cell Ph"}],"container-title":["Journal of the Association for Research in Otolaryngology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10162-025-00986-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10162-025-00986-1\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10162-025-00986-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,3]],"date-time":"2025-06-03T16:28:35Z","timestamp":1748968115000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10162-025-00986-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,16]]},"references-count":42,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2025,6]]}},"alternative-id":["986"],"URL":"https:\/\/doi.org\/10.1007\/s10162-025-00986-1","relation":{},"ISSN":["1438-7573"],"issn-type":[{"value":"1438-7573","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,16]]},"assertion":[{"value":"6 December 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"27 March 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"16 April 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of Interest"}}]}}