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Since it relies on mechanical intervention without genetic or pharmacological manipulations, it is a good model for understanding the biomechanics origins of such HLHS malformations. To date, however, the fluid mechanical environment of this model is poorly understood. In the current study, we performed 4D ultrasound imaging of LAL and normal chick embryonic hearts and 4D cardiac flow simulations to help shed light on the mechanical environment that may lead to the HLHS morphology. Results showed that the HH25 LAL atrial function was compromised, and velocities in the ventricle were reduced. The HH25 LAL ventricles developed a more triangular shape with a sharper apex, and in some cases, the atrioventricular junction shifted medially. These changes led to more sluggish flow near the ventricular free wall and apex, where more fluid particles moved in an oscillatory manner with the motion of the ventricular wall, while slowly being washed out, resulting in lower wall shear stresses and higher oscillatory indices. Consequent to these flow conditions, at HH28, even before septation is complete, the left ventricle was found to be hypoplastic while the right ventricle was found to be larger in compensation. Our results suggest that the low and oscillatory flow near the left side of the heart may play a role in causing the HLHS morphology in the LAL model.<\/jats:p>","DOI":"10.1007\/s10237-021-01447-3","type":"journal-article","created":{"date-parts":[[2021,3,28]],"date-time":"2021-03-28T12:02:45Z","timestamp":1616932965000},"page":"1337-1351","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Fluid mechanics of the left atrial ligation chick embryonic model of hypoplastic left heart syndrome"],"prefix":"10.1007","volume":"20","author":[{"given":"Sheldon","family":"Ho","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei Xuan","family":"Chan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2918-3077","authenticated-orcid":false,"given":"Choon Hwai","family":"Yap","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,3,28]]},"reference":[{"key":"1447_CR7","doi-asserted-by":"publisher","first-page":"341","DOI":"10.1016\/0167-5273(89)90226-X)","volume":"25","author":"LD Allan","year":"1989","unstructured":"Allan LD, Sharland G, Tynan MJ (1989) The natural history of the hypoplastic left heart syndrome. 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