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The florets are arranged in a regular phyllotactic pattern with Fibonacci numbers of left- and right-winding spirals. Such a pattern may be disrupted due to physical constraints or by wounding occurring during the early meristem development. Recovery from wounding re-establishes patterning although the mechanisms have remained elusive. In this study, we applied <jats:italic>Gerbera hybrida<\/jats:italic> as a model system and established methods to conduct wounding experiments either with syringe needles or using laser ablation combined with live imaging of head meristems. By revisiting the historical experiments in sunflower, we conducted wounding to transgenic auxin reporter lines of gerbera and followed the recovery of cellular growth and meristem patterning. We show that wounding disrupted the expression of the gerbera <jats:italic>CLAVATA3<\/jats:italic> (<jats:italic>GhCLV3<\/jats:italic>) gene that marks the undifferentiated meristematic region and led to <jats:italic>de novo<\/jats:italic> re-initiation of patterning at the wound margin. During the recovery growth, three to five layers of elongated cells showing periclinal cell division planes and lacking auxin signal were formed at the wound rim. DR5 auxin signal was shown to localize and form regularly spaced maxima in a distance from the wound rim. Consequently, spiral pattern of contact parastichies was re-established by stacking of new auxin maxima on top of the previous ones. The developed methods facilitate future studies on understanding the molecular mechanisms of <jats:italic>de novo<\/jats:italic> patterning of meristems.<\/jats:p>","DOI":"10.1007\/s10265-021-01253-z","type":"journal-article","created":{"date-parts":[[2021,2,4]],"date-time":"2021-02-04T17:38:43Z","timestamp":1612460323000},"page":"431-440","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Repatterning of the inflorescence meristem in Gerbera hybrida after wounding"],"prefix":"10.1007","volume":"134","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5189-3289","authenticated-orcid":false,"given":"Teng","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8377-4356","authenticated-orcid":false,"given":"Feng","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6512-0810","authenticated-orcid":false,"given":"Paula","family":"Elomaa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,2,4]]},"reference":[{"key":"1253_CR1","first-page":"5","volume":"8","author":"FPC Blamey","year":"1976","unstructured":"Blamey FPC (1976) Boron nutrition of sunflowers (Helianthus annuus L.) on an Avalon medium sandy loam. 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