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This study examined the morphological and reproductive characteristics of triploid\n                    <jats:italic>Passiflora cincinnata<\/jats:italic>\n                    Masters, with a focus on fruit and seed production. Triploid plants were obtained through in vitro cultivation of the endosperm via somatic embryogenesis, whereas diploid plants were raised through seed germination. Diploid and triploid plants were confirmed by flow cytometry and cytogenetic analyses. Cross-pollination and self-pollination yielded, on average, 18 fruits per plant in the cytotype diploid, while triploids produced only 6 fruits. Diploid plants have more uniform fruits, while triploid plants showed variations in size. Seeds with malformations or absence of zygotic embryos and endosperm were observed in triploid cytotypes, while remaining below 1.5% in diploid cytotypes. It was observed that the highest germination rate of 80% occurred in seeds of the diploid cytotype, compared to 53.3% in triploids. The seedlings from the cross between diploid plants contained 3.29\u00a0pg of nuclear DNA and a chromosome number of 2n\u2009=\u20092x\u2009=\u200918, while the seedlings from the cross between triploid plants had progeny with 3.37\u00a0pg of nuclear DNA and variable chromosomes numbers, of 2n\u2009=\u200919 (2n\u2009+\u20091), 2n\u2009=\u200915 (2n\u2009\u2212\u20093), and 2n\u2009=\u200916 (2n\u2009\u2212\u20092) chromosomes confirming aneuploidy in the progeny. Stomatal analysis showed that diploid plants displayed the highest stomatal density, whereas triploid and aneuploid plants had the greatest length and width of stomata. The density and width of pavement epidermal cells varied among cytotypes. Meiotic disturbances in triploid plants cause phenotypic alterations in the progeny, resulting in delayed vegetative development, low vigor, non-uniform germination, and death during the juvenile phase. The genetic material of aneuploids is a resource for gene mapping, enabling the modification or production of new cultivars with economically relevant phenotypes.\n                  <\/jats:p>","DOI":"10.1007\/s10265-026-01695-3","type":"journal-article","created":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T13:08:34Z","timestamp":1773061714000},"page":"479-492","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Fruit and seed attributes of plants derived from crossing between triploid Passionflower cytotypes"],"prefix":"10.1007","volume":"139","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6701-9193","authenticated-orcid":false,"given":"Claudinei","family":"da Silva Souza","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5419-7003","authenticated-orcid":false,"given":"Marcelo Dias","family":"Machado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-9946-6809","authenticated-orcid":false,"given":"Alana Jeniffer Alves","family":"dos Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-7186-7154","authenticated-orcid":false,"given":"Gabrielle Balbo","family":"Crepaldi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3466-4927","authenticated-orcid":false,"given":"Elyabe Monteiro","family":"de Matos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7575-8241","authenticated-orcid":false,"given":"Lyderson Facio","family":"Viccini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1657-348X","authenticated-orcid":false,"given":"Ilio Fealho","family":"de Carvalho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9614-9373","authenticated-orcid":false,"given":"Wagner Campos","family":"Otoni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2419-9996","authenticated-orcid":false,"given":"Aryane Campos","family":"Reis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6683-0961","authenticated-orcid":false,"given":"Diego Ismael","family":"Rocha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6928-285X","authenticated-orcid":false,"given":"Maurecilne Lemes","family":"da Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,3,9]]},"reference":[{"key":"1695_CR1","doi-asserted-by":"publisher","first-page":"1023","DOI":"10.1007\/s00299-010-0888-7","volume":"29","author":"P Aleza","year":"2010","unstructured":"Aleza P, Ju\u00e1rez J, Cuenca J, Ollitrault P, Navarro L (2010) Recovery of citrus triploid hybrids by embryo rescue and flow cytometry from 2x \u00d7 2x sexual hybridisation and its application to extensive breeding programs. 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