{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T09:37:50Z","timestamp":1777455470407,"version":"3.51.4"},"reference-count":29,"publisher":"Springer Science and Business Media LLC","issue":"2","license":[{"start":{"date-parts":[[2018,6,29]],"date-time":"2018-06-29T00:00:00Z","timestamp":1530230400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"funder":[{"name":"International Dental Collaboration of the Mekong River Region (IDCMR) scholarship from the Faculty of Dentistry, Mahidol University"},{"name":"Office of the Higher Education Commission and Mahidol University under the National Research Universities Initiative, Thailand"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Odontology"],"published-print":{"date-parts":[[2019,4]]},"DOI":"10.1007\/s10266-018-0374-1","type":"journal-article","created":{"date-parts":[[2018,6,29]],"date-time":"2018-06-29T07:47:54Z","timestamp":1530258474000},"page":"133-141","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Oxidative stress induced by Porphyromonas gingivalis lysate and nicotine in human periodontal ligament fibroblasts"],"prefix":"10.1007","volume":"107","author":[{"given":"Thuy Thu","family":"Nguyen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nam Nhat-Cong","family":"Huynh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sujiwan","family":"Seubbuk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thanapoj","family":"Nilmoje","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aree","family":"Wanasuntronwong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rudee","family":"Surarit","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2018,6,29]]},"reference":[{"key":"374_CR1","doi-asserted-by":"publisher","first-page":"1426","DOI":"10.1002\/jcb.24017","volume":"113","author":"Y Choe","year":"2012","unstructured":"Choe Y, Yu JY, Son YO, Park SM, Kim JG, Shi X, Lee JC. Continuously generated H2O2 stimulates the proliferation and osteoblastic differentiation of human periodontal ligament fibroblasts. J Cell Biochem. 2012;113:1426\u201336.","journal-title":"J Cell Biochem"},{"key":"374_CR2","doi-asserted-by":"publisher","first-page":"164","DOI":"10.1111\/j.1600-0757.2005.00139.x","volume":"40","author":"PM Bartold","year":"2006","unstructured":"Bartold PM, Shi S, Gronthos S. Stem cells and periodontal regeneration. Periodontol 2000. 2006;40:164\u201372.","journal-title":"Periodontol 2000"},{"key":"374_CR3","doi-asserted-by":"publisher","first-page":"23070","DOI":"10.1074\/jbc.M611181200","volume":"282","author":"S Yamada","year":"2007","unstructured":"Yamada S, Tomoeda M, Ozawa Y, Yoneda S, Terashima Y, Ikezawa K, Ikegawa S, Saito M, Toyosawa S, Murakami S. PLAP-1\/asporin, a novel negative regulator of periodontal ligament mineralization. J Biol Chem. 2007;282:23070\u201380.","journal-title":"J Biol Chem"},{"key":"374_CR4","first-page":"129","volume":"62","author":"G Thornton-Evans","year":"2013","unstructured":"Thornton-Evans G, Eke P, Wei L, Palmer A, Moeti R, Hutchins S, Borrell LN. Centers for disease control and prevention (CDC). Periodontitis among adults aged \u2265 30 years\u2014United States, 2009\u20132010. MMWR Suppl. 2013;62:129\u201335.","journal-title":"MMWR Suppl"},{"key":"374_CR5","doi-asserted-by":"publisher","first-page":"1153","DOI":"10.1016\/j.archoralbio.2015.05.004","volume":"60","author":"J Liu","year":"2015","unstructured":"Liu J, Tang X, Li C, Pan C, Li Q, Geng F, Pan Y. Porphyromonas gingivalis promotes the cell cycle and inflammatory cytokine production in periodontal ligament fibroblasts. Arch Oral Biol. 2015;60:1153\u201361.","journal-title":"Arch Oral Biol"},{"key":"374_CR6","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/s10266-004-0043-4","volume":"92","author":"J Bergstr\u00f6m","year":"2004","unstructured":"Bergstr\u00f6m J. Tobacco smoking and chronic destructive periodontal disease. Odontology. 2004;92:1\u20138.","journal-title":"Odontology"},{"key":"374_CR7","doi-asserted-by":"publisher","first-page":"356","DOI":"10.1177\/10454411000110030501","volume":"11","author":"DF Kinane","year":"2000","unstructured":"Kinane DF, Chestnutt IG. Smoking and periodontal disease. Crit Rev Oral Biol Med. 2000;11:356\u201365.","journal-title":"Crit Rev Oral Biol Med"},{"key":"374_CR8","doi-asserted-by":"publisher","first-page":"279","DOI":"10.1034\/j.1600-0765.2002.01612.x","volume":"37","author":"YC Chang","year":"2002","unstructured":"Chang YC, Huang FM, Tai KW, Yang LC, Chou MY. Mechanisms of cytotoxicity of nicotine in human periodontal ligament fibroblast cultures in vitro. J Periodontal Res. 2002;37:279\u201385.","journal-title":"J Periodontal Res"},{"key":"374_CR9","doi-asserted-by":"publisher","first-page":"1269","DOI":"10.1902\/jop.1999.70.11.1269","volume":"70","author":"NM Sayers","year":"1999","unstructured":"Sayers NM, James JA, Drucker DB, Blinkhorn AS. Possible potentiation of toxins from Prevotella intermedia, Prevotella nigrescens, and Porphyromonas gingivalis by cotinine. J Periodontol. 1999;70:1269\u201375.","journal-title":"J Periodontol"},{"key":"374_CR10","doi-asserted-by":"publisher","first-page":"986264","DOI":"10.1155\/2014\/986264","volume":"2014","author":"L G\u00f6lz","year":"2014","unstructured":"G\u00f6lz L, Memmert S, Rath-Deschner B, J\u00e4ger A, Appel T, Baumgarten G. LPS from P. gingivalis and hypoxia increases oxidative stress in periodontal ligament fibroblasts and contributes to periodontitis. Mediators Inflamm. 2014;2014:986264.","journal-title":"Mediators Inflamm"},{"key":"374_CR11","doi-asserted-by":"publisher","first-page":"713","DOI":"10.1902\/jop.2013.130066","volume":"85","author":"S Trivedi","year":"2014","unstructured":"Trivedi S, Lal N, Mahdi AA, Mittal M, Singh B, Pandey S. Evaluation of antioxidant enzymes activity and malondialdehyde levels in patients with chronic periodontitis and diabetes mellitus. J Periodontol. 2014;85:713\u201320.","journal-title":"J Periodontol"},{"key":"374_CR12","doi-asserted-by":"publisher","first-page":"682","DOI":"10.1902\/jop.2015.140561","volume":"86","author":"\u00d6 Fento\u011flu","year":"2015","unstructured":"Fento\u011flu \u00d6, K\u0131rz\u0131o\u011flu FY, Bulut MT, Kumbul Do\u011fu\u00e7 D, Kula\u00e7 E, \u00d6nder C, G\u00fcnhan M. Evaluation of lipid peroxidation and oxidative DNA damage in patients with periodontitis and hyperlipidemia. J Periodontol. 2015;86:682\u20138.","journal-title":"J Periodontol"},{"key":"374_CR13","first-page":"931083","volume":"2014","author":"Z Liu","year":"2014","unstructured":"Liu Z, Liu Y, Song Y, Zhang X, Wang S, Wang Z. Systemic oxidative stress biomarkers in chronic periodontitis: a meta-analysis. Dis Markers. 2014;2014:931083.","journal-title":"Dis Markers"},{"key":"374_CR14","doi-asserted-by":"publisher","first-page":"42","DOI":"10.3109\/00016357.2013.795659","volume":"72","author":"D Miricescu","year":"2014","unstructured":"Miricescu D, Totan A, Calenic B, Mocanu B, Didilescu A, Mohora M, Spinu T, Greabu M. Salivary biomarkers: relationship between oxidative stress and alveolar bone loss in chronic periodontitis. Acta Odontol Scand. 2014;72:42\u20137.","journal-title":"Acta Odontol Scand"},{"key":"374_CR15","doi-asserted-by":"publisher","first-page":"70","DOI":"10.1111\/j.1834-7819.2009.01123.x","volume":"55","author":"D Wei","year":"2010","unstructured":"Wei D, Zhang XL, Wang YZ, Yang CX, Chen G. Lipid peroxidation levels, total oxidant status and superoxide dismutase in serum, saliva and gingival crevicular fluid in chronic periodontitis patients before and after periodontal therapy. Aust Dent J. 2010;55:70\u20138.","journal-title":"Aust Dent J"},{"key":"374_CR16","doi-asserted-by":"publisher","first-page":"134","DOI":"10.1902\/jop.2015.150353","volume":"87","author":"TT Nguyen","year":"2016","unstructured":"Nguyen TT, Ngo LQ, Promsudthi A, Surarit R. Salivary lipid peroxidation in patients with generalized chronic periodontitis and acute coronary syndrome. J Periodontol. 2016;87:134\u201341.","journal-title":"J Periodontol"},{"key":"374_CR17","doi-asserted-by":"publisher","first-page":"351","DOI":"10.1016\/0003-2697(79)90738-3","volume":"95","author":"H Ohkawa","year":"1979","unstructured":"Ohkawa H, Ohishi N, Yaki K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979;95:351\u20138.","journal-title":"Anal Biochem"},{"key":"374_CR18","doi-asserted-by":"publisher","first-page":"716","DOI":"10.1016\/j.archoralbio.2008.02.002","volume":"53","author":"E Baltacioglu","year":"2008","unstructured":"Baltacioglu E, Akalin FA, Alver A, Deger O, Karabulut E. Protein carbonyl levels in serum and gingival crevicular fluid in patients with chronic periodontitis. Arch Oral Biol. 2008;53:716\u201322.","journal-title":"Arch Oral Biol"},{"key":"374_CR19","doi-asserted-by":"publisher","first-page":"167","DOI":"10.1042\/CS20030031","volume":"105","author":"DV Sculley","year":"2003","unstructured":"Sculley DV, Langley-Evans SC. Periodontal disease is associated with lower antioxidant capacity in whole saliva and evidence of increased protein oxidation. Clin Sci (Lond). 2003;105:167\u201372.","journal-title":"Clin Sci (Lond)"},{"key":"374_CR20","first-page":"241","volume":"22","author":"YH Lee","year":"2009","unstructured":"Lee YH, Wong DT. Saliva: an emerging biofluid for early detection of diseases. Am J Dent. 2009;22:241\u20138.","journal-title":"Am J Dent"},{"key":"374_CR21","first-page":"170","volume":"68","author":"HP Lawrence","year":"2002","unstructured":"Lawrence HP. Salivary markers of systemic disease: noninvasive diagnosis of disease and monitoring of general health. J Can Dent Assoc. 2002;68:170\u20134.","journal-title":"J Can Dent Assoc"},{"key":"374_CR22","doi-asserted-by":"publisher","first-page":"1043","DOI":"10.1136\/bmj.1.6017.1043","volume":"1","author":"MA Russell","year":"1976","unstructured":"Russell MA, Feyerabend C, Cole PV. Plasma nicotine levels after cigarette smoking and chewing nicotine gum. Br Med J. 1976;1:1043\u20136.","journal-title":"Br Med J"},{"key":"374_CR23","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1007\/978-3-540-69248-5_2","volume":"192","author":"NL Benowitz","year":"2009","unstructured":"Benowitz NL, Hukkanen J, Jacob P. Nicotine chemistry, metabolism, kinetics and biomarkers. Handb Exp Pharmacol. 2009;192:29\u201360.","journal-title":"Handb Exp Pharmacol"},{"key":"374_CR24","doi-asserted-by":"publisher","first-page":"1002","DOI":"10.1136\/bmj.284.6321.1002","volume":"284","author":"C Feyerabend","year":"1982","unstructured":"Feyerabend C, Higenbottam T, Russell MA. Nicotine concentrations in urine and saliva of smokers and non-smokers. Br Med J. 1982;284:1002\u20134.","journal-title":"Br Med J"},{"issue":"5","key":"374_CR25","doi-asserted-by":"publisher","first-page":"641","DOI":"10.1902\/jop.2001.72.5.641","volume":"72","author":"Z Chen","year":"2001","unstructured":"Chen Z, Casiano CA, Fletcher HM. Protease-active extracellular protein preparations from Porphyromonas gingivalis W83 induce N-cadherin proteolysis, loss of cell adhesion, and apoptosis in human epithelial cells. J Periodontol. 2001;72(5):641\u201350.","journal-title":"J Periodontol"},{"issue":"12","key":"374_CR26","doi-asserted-by":"publisher","first-page":"6968","DOI":"10.1128\/IAI.70.12.6968-6975.2002","volume":"70","author":"MA Curtis","year":"2002","unstructured":"Curtis MA, Aduse Opoku J, Rangarajan M, Gallagher A, Sterne JA, Reid CR, et al. Attenuation of the virulence of Porphyromonas gingivalis by using a specific synthetic Kgp protease inhibitor. Infect Immun. 2002;70(12):6968\u201375.","journal-title":"Infect Immun"},{"key":"374_CR27","doi-asserted-by":"publisher","first-page":"1007","DOI":"10.3892\/ijmm.2012.1102","volume":"30","author":"T Kiyoshima","year":"2012","unstructured":"Kiyoshima T, Enoki N, Kobayashi I, Sakai T, Nagata K, Wada H, Fujiwara H, Ookuma Y, Sakai H. Oxidative stress caused by a low concentration of hydrogen peroxide induces senescence-like changes in mouse gingival fibroblasts. Int J Mol Med. 2012;30:1007\u201312.","journal-title":"Int J Mol Med"},{"key":"374_CR28","first-page":"29","volume":"23","author":"AD Romano","year":"2010","unstructured":"Romano AD, Serviddio G, De Matthaeis A, Bellanti F, Vendemiale G. Oxidative stress and aging. J Nephrol. 2010;23:29\u201336.","journal-title":"J Nephrol"},{"key":"374_CR29","first-page":"794","volume":"52","author":"TP Devasagayam","year":"2004","unstructured":"Devasagayam TP, Tilak JC, Boloor KK, Sane KS, Ghaskadbi SS, Lele RD. Free radicals and antioxidants in human health: current status and future prospects. J Assoc Physicians India. 2004;52:794\u2013804.","journal-title":"J Assoc Physicians India"}],"container-title":["Odontology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s10266-018-0374-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s10266-018-0374-1\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s10266-018-0374-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,8,29]],"date-time":"2019-08-29T14:27:59Z","timestamp":1567088879000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s10266-018-0374-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,6,29]]},"references-count":29,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2019,4]]}},"alternative-id":["374"],"URL":"https:\/\/doi.org\/10.1007\/s10266-018-0374-1","relation":{},"ISSN":["1618-1247","1618-1255"],"issn-type":[{"value":"1618-1247","type":"print"},{"value":"1618-1255","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,6,29]]},"assertion":[{"value":"29 January 2018","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"24 June 2018","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 June 2018","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with ethical standards"}},{"value":"The authors declare that they have no conflict of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"Ethical approval is not required since cell lines used in this current study were derived from expansion of primary cell cultures in vitro and are not considered relevant to the requirement.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}}]}}