{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T02:43:15Z","timestamp":1790908995223,"version":"4.1.0"},"reference-count":42,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2018,7,23]],"date-time":"2018-07-23T00:00:00Z","timestamp":1532304000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Odontology"],"published-print":{"date-parts":[[2019,1]]},"DOI":"10.1007\/s10266-018-0380-3","type":"journal-article","created":{"date-parts":[[2018,7,23]],"date-time":"2018-07-23T14:29:24Z","timestamp":1532356164000},"page":"54-63","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":50,"title":["Evaluation of the biocompatibility of root canal sealers on human periodontal ligament cells ex vivo"],"prefix":"10.1007","volume":"107","author":[{"given":"Susanne","family":"Jung","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viktor","family":"Libricht","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonja","family":"Sielker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcel R.","family":"Hanisch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edgar","family":"Sch\u00e4fer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1276-0632","authenticated-orcid":false,"given":"Till","family":"Dammaschke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2018,7,23]]},"reference":[{"key":"380_CR1","first-page":"343","volume-title":"Grossman\u2019s Endodntic Practice","author":"B Suresh Chandra","year":"2014","unstructured":"Suresh Chandra B, Gopikrishna V. Obturation of the radicular space. In: Suresh Chandra B, Gopikrishna V, editors. Grossman\u2019s Endodntic Practice. 13th ed. New Dehli: Wolters Kluwer Health; 2014. pp.\u00a0343\u201373.","edition":"13"},{"key":"380_CR2","first-page":"280","volume-title":"Cohen\u2019s Pathway of the Pulp","author":"W Johnson","year":"2016","unstructured":"Johnson W, Kulild JC, Tay F. Obturation of the cleaned and shaped root canal system. In: Hargreaves KH, Berman LH, editors. Cohen\u2019s Pathway of the Pulp. 11th ed. St. Louis: Elsevier; 2016. pp.\u00a0280\u2013323.","edition":"11"},{"key":"380_CR3","doi-asserted-by":"publisher","first-page":"359","DOI":"10.1016\/S1079-2104(05)80230-6","volume":"79","author":"D Granchi","year":"1995","unstructured":"Granchi D, Stea S, Ciapetti G, Cavedagna D, Stea S, Pizzoferrato A. Endodontic cements induce alterations in the cell cycle of in vitro cultured osteoblasts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1995;79:359\u201366.","journal-title":"Oral Surg Oral Med Oral Pathol Oral Radiol Endod"},{"key":"380_CR4","first-page":"699","volume":"35","author":"T Dammaschke","year":"2004","unstructured":"Dammaschke T, Witt M, Ott K, Sch\u00e4fer E. Scanning electron microscopic investigation of incidence, location, and size of accessory foramina in primary and permanent molars. Quintessence Int. 2004;35:699\u2013705.","journal-title":"Quintessence Int"},{"key":"380_CR5","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1111\/j.1747-4477.2001.tb00445.x","volume":"27","author":"W Geurtsen","year":"2001","unstructured":"Geurtsen W. Biocompatibility of root canal filling materials. Aust Endod J. 2001;27:12\u201321.","journal-title":"Aust Endod J"},{"key":"380_CR6","doi-asserted-by":"publisher","first-page":"390","DOI":"10.1111\/edt.12190","volume":"31","author":"JM Braga","year":"2015","unstructured":"Braga JM, Oliveira RR, de Castro Mantins R, Vieira LQ, Sobrinho AP. Assessment of the cytotoxicity of a mineral trioxide aggregate-based sealer with respect to macrophage activity. Dent Traumatol. 2015;31:390\u20135.","journal-title":"Dent Traumatol"},{"key":"380_CR7","doi-asserted-by":"publisher","first-page":"233","DOI":"10.1111\/j.1600-9657.1994.tb00076.x","volume":"10","author":"J Tepel","year":"1994","unstructured":"Tepel J, Darwisch el Sawaf M, Hoppe W. Reaction of inflamed periapical tissue to intracanal medicaments and root canal sealers. Endod Dent Traumatol. 1994;10:233\u20138.","journal-title":"Endod Dent Traumatol"},{"key":"380_CR8","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1016\/j.joen.2015.09.020","volume":"42","author":"F Costa","year":"2016","unstructured":"Costa F, Sousa Gomes P, Fernandes MH. Osteogenic and angiogenic response to calcium silicate-based endodontic sealers. J Endod. 2016;42:113\u20139.","journal-title":"J Endod"},{"key":"380_CR9","first-page":"133","volume":"77","author":"L Sp\u00e5ngberg","year":"1969","unstructured":"Sp\u00e5ngberg L. Biological effects of root canal filling materials. 7. Reaction of bony tissue to implanted root canal filling material in guinea pigs. Odontol Tidskr. 1969;77:133\u201359.","journal-title":"Odontol Tidskr"},{"key":"380_CR10","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1002\/9781118892435.ch10","volume-title":"Mineral Trioxide Aggregate. Properties and clinical applications","author":"M Parirokh","year":"2014","unstructured":"Parirokh M, Torabinejad M. Calcium silicate-based cements. In: Torabinejad M, editor. Mineral Trioxide Aggregate. Properties and clinical applications. Ames: Wiley Blackwell; 2014. pp.\u00a0281\u2013332."},{"key":"380_CR11","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1016\/j.joen.2014.09.015","volume":"41","author":"M Xuereb","year":"2015","unstructured":"Xuereb M, Vella P, Damidot D, Sammut CV, Camilleri J. In situ assessment of the setting of tricalcium silicate-based sealers using a dentin pressure model. J Endod. 2015;41:111\u201324.","journal-title":"J Endod"},{"key":"380_CR12","doi-asserted-by":"publisher","first-page":"245","DOI":"10.1007\/s10266-017-0329-y","volume":"106","author":"G Szczurko","year":"2018","unstructured":"Szczurko G, Pawi\u0144ska M, \u0141uczaj-Cepowicz KA, Marczuk-Kolada G, Ho\u0142ownia A. Effect of root canal sealers on human periodontal ligament fibroblast viability: ex vivo study. Odontology. 2018;106:245\u201356.","journal-title":"Odontology"},{"key":"380_CR13","doi-asserted-by":"publisher","first-page":"361","DOI":"10.1067\/moe.2002.126341","volume":"94","author":"FM Huang","year":"2002","unstructured":"Huang FM, Chang YC. Cytotoxicity of resin-based restorative materials on human pulp cell cultures. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2002;94:361\u20135.","journal-title":"Oral Surg Oral Med Oral Pathol Oral Radiol Endod"},{"key":"380_CR14","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1186\/s13005-014-0055-4","volume":"10","author":"S Jung","year":"2014","unstructured":"Jung S, Mielert J, Kleinheinz J, Dammaschke T. Human oral cells\u2019 response to different endodontic restorative materials: an in vitro study. Head Face Med. 2014;10:55. https:\/\/doi.org\/10.1186\/s13005-014-0055-4 .","journal-title":"Head Face Med"},{"key":"380_CR15","doi-asserted-by":"publisher","first-page":"e0194467","DOI":"10.1371\/journal.pone.0194467","volume":"13","author":"S Jung","year":"2018","unstructured":"Jung S, Sielker S, Hanisch MR, Libricht V, Sch\u00e4fer E, Dammaschke T. Cytotoxic effects of four different root canal sealers on human osteoblasts. PLoS One. 2018;13:e0194467. https:\/\/doi.org\/10.1371\/journal.pone.0194467 .","journal-title":"PLoS One"},{"key":"380_CR16","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1186\/1746-160X-7-15","volume":"7","author":"I Willershausen","year":"2011","unstructured":"Willershausen I, Callaway A, Brise\u00f1o B, Willershausen B. In vitro analysis of the cytotoxicity and the antimicrobial effect of four endodontic sealers. Head Face Med. 2011;7:15. https:\/\/doi.org\/10.1186\/1746-160X-7-15 .","journal-title":"Head Face Med"},{"key":"380_CR17","doi-asserted-by":"publisher","first-page":"1469","DOI":"10.1016\/j.joen.2015.04.011","volume":"41","author":"J Camps","year":"2015","unstructured":"Camps J, Jeanneau C, El Ayachi I, Laurent P, About I. Bioactivity of a calcium silicate-based endodontic cement (BioRoot RCS): interaction with human periodontal ligament cells in vitro. J Endod. 2015;41:1469\u201373.","journal-title":"J Endod"},{"key":"380_CR18","doi-asserted-by":"publisher","first-page":"875","DOI":"10.1111\/iej.12703","volume":"50","author":"M Collado-Gonz\u00e1lez","year":"2017","unstructured":"Collado-Gonz\u00e1lez M, Garc\u00eda-Bernal D, O\u00f1ate-S\u00e1nchez RE, Ortolani-Seltenerich PS, Lozano A, Forner L, Llena C, Rodr\u00edguez-Lozano FJ. Biocompatibility of three new calcium silicate-based endodontic sealers on human periodontal ligament stem cells. Int Endod J. 2017;50:875\u201384.","journal-title":"Int Endod J"},{"key":"380_CR19","doi-asserted-by":"publisher","first-page":"638","DOI":"10.1097\/00004770-200209000-00003","volume":"28","author":"E Sch\u00e4fer","year":"2002","unstructured":"Sch\u00e4fer E, Olthoff G. Effect of three different sealers on the sealing ability of both Thermafil obturators and cold laterally compacted gutta-percha. J Endod. 2002;28:638\u201342.","journal-title":"J Endod"},{"key":"380_CR20","doi-asserted-by":"publisher","first-page":"660","DOI":"10.1046\/j.1365-2591.2003.00705.x","volume":"36","author":"E Sch\u00e4fer","year":"2003","unstructured":"Sch\u00e4fer E, Zandbiglari T. Solubility of root canal sealers in water and artificial saliva. Int Endod J. 2003;36:660\u20139.","journal-title":"Int Endod J"},{"key":"380_CR21","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1007\/s10266-013-0137-y","volume":"103","author":"E Sch\u00e4fer","year":"2015","unstructured":"Sch\u00e4fer E, Bering N, B\u00fcrklein S. Selected physicochemical properties of AH-Plus, EndoREZ and RealSeal SE root canal sealers. Odontology. 2015;103:61\u20135.","journal-title":"Odontology"},{"key":"380_CR22","doi-asserted-by":"publisher","first-page":"816","DOI":"10.1016\/j.joen.2017.01.001","volume":"43","author":"M Collado-Gonz\u00e1lez","year":"2017","unstructured":"Collado-Gonz\u00e1lez M, Tom\u00e1s-Catal\u00e1 CJ, O\u00f1ate-S\u00e1nchez RE, Moraleda JM, Rodr\u00edguez-Lozano J. Cytotoxicity of GuttaFlow Bioseal, GuttaFlow 2, MTA Fillapex, and AH Plus on human periodontal ligament stem cells. J Endod. 2017;43:816\u201322.","journal-title":"J Endod"},{"key":"380_CR23","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1007\/s007840050057","volume":"2","author":"H Schweikl","year":"1998","unstructured":"Schweikl H, Schmalz G, Federlin M. Mutagenicity of the root canal sealer AH Plus in the Ames test. Clin Oral Investig. 1998;2:125\u20139.","journal-title":"Clin Oral Investig"},{"key":"380_CR24","doi-asserted-by":"publisher","first-page":"462","DOI":"10.1097\/00004770-200008000-00008","volume":"26","author":"NG Azar","year":"2000","unstructured":"Azar NG, Heidari M, Bahrami ZS, Shokri F. In vitro cytotoxicity of a new epoxy resin root canal sealer. J Endod. 2000;26:462\u20136.","journal-title":"J Endod"},{"key":"380_CR25","doi-asserted-by":"publisher","first-page":"656","DOI":"10.1016\/j.joen.2005.12.003","volume":"32","author":"CJA Sousa","year":"2006","unstructured":"Sousa CJA, Montes CRM, Pasco EA, Loyola AM, Versiami MA. Comparison of the intraosseous biocompatibility of AH Plus, Endo REZ, and Epiphany root canal sealer. J Endod. 2006;32:656\u201362.","journal-title":"J Endod"},{"key":"380_CR26","first-page":"39","volume-title":"Pathologie","author":"K Zatloukal","year":"2004","unstructured":"Zatloukal K, Roth J, Denk H. Zell- und Gewebereaktionen. In: B\u00f6cker W, Denk H, Heitz PU, editors. Pathologie. 3rd ed. M\u00fcnchen: Urban & Fischer; 2004. pp.\u00a039\u201376.","edition":"3"},{"key":"380_CR27","doi-asserted-by":"publisher","first-page":"1615","DOI":"10.1177\/00220345010800070401","volume":"80","author":"H Schweikl","year":"2001","unstructured":"Schweikl H, Schmalz G, Spruss T. The induction of micronuclei in vitro by unpolymerized resin monomers. J Dent Res. 2001;80:1615\u201320.","journal-title":"J Dent Res"},{"key":"380_CR28","first-page":"37","volume":"88","author":"BI Cohen","year":"1998","unstructured":"Cohen BI, Pagnillo MK, Musikant BL, Deutsch AS. Formaldehyde from endodontic materials. Oral Health. 1998;88:37\u20139.","journal-title":"Oral Health"},{"key":"380_CR29","doi-asserted-by":"publisher","first-page":"329","DOI":"10.1111\/j.1365-2591.2007.01211.x","volume":"40","author":"AU Eldeniz","year":"2007","unstructured":"Eldeniz AU, Mustafa K, \u00d8rstavik D, Dahl JE. Cytotoxicity of new resin-, calcium hydroxide- and silicone-based root canal sealers on fibroblasts derived from human gingiva and L929 cell lines. Int Endod J. 2007;40:329\u201337.","journal-title":"Int Endod J"},{"key":"380_CR30","doi-asserted-by":"publisher","first-page":"56","DOI":"10.1016\/j.joen.2014.09.012","volume":"41","author":"H-M Zhou","year":"2015","unstructured":"Zhou H-M, Du T-F, Shen Y, Wang Z-J, Zheng Y-F, Haapasalo M. In vitro cytotoxicity of calcium silicate-containing endodontic sealers. J Endod. 2015;41:56\u201361.","journal-title":"J Endod"},{"key":"380_CR31","doi-asserted-by":"publisher","first-page":"419","DOI":"10.1590\/0103-6440201600735","volume":"27","author":"EJ Silva","year":"2016","unstructured":"Silva EJ, Accorsi-Mendon\u00e7a T, Pedrosa AC, Granjeiro JM, Zaia AA. Long-term cytotoxicity, pH and dissolution rate of AH Plus and MTA Fillapex. Braz Dent J. 2016;27:419\u201323.","journal-title":"Braz Dent J"},{"key":"380_CR32","doi-asserted-by":"publisher","first-page":"274","DOI":"10.1016\/j.joen.2012.06.030","volume":"39","author":"EJ Silva","year":"2013","unstructured":"Silva EJ, Rosa TP, Herrera DR, Jacinto RC, Gomes BP, Zaia AA. Evaluation of cytotoxicity and physicochemical properties of calcium silicate-based endodontic sealer MTA Fillapex. J Endod. 2013;39:274\u20137.","journal-title":"J Endod"},{"key":"380_CR33","doi-asserted-by":"publisher","first-page":"148","DOI":"10.1111\/j.1365-2591.2009.01669.x","volume":"43","author":"AS Al-Hiyasat","year":"2010","unstructured":"Al-Hiyasat AS, Tayyar M, Darmani H. Cytotoxicity evaluation of various resin based root canal sealers. Int Endod J. 2010;43:148\u201353.","journal-title":"Int Endod J"},{"key":"380_CR34","doi-asserted-by":"publisher","first-page":"1141","DOI":"10.1111\/iej.12577","volume":"49","author":"AU Eldeniz","year":"2016","unstructured":"Eldeniz AU, Shehata M, H\u00f6gg C, Reichl FX. DNA double-strand breaks caused by new and contemporary endodontic sealers. Int Endod J. 2016;49:1141\u201351.","journal-title":"Int Endod J"},{"key":"380_CR35","first-page":"15","volume":"61","author":"T Dammaschke","year":"2006","unstructured":"Dammaschke T, Schneider U, Stratmann U, Yoo J-M, Sch\u00e4fer E. Reaction of inflamed periapical tissue to three different root canal sealers. Dtsch Zahn\u00e4rztl Z. 2006;61:15\u201326.","journal-title":"Dtsch Zahn\u00e4rztl Z"},{"key":"380_CR36","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1111\/iej.12596","volume":"50","author":"FJ Rodr\u00edguez-Lozano","year":"2017","unstructured":"Rodr\u00edguez-Lozano FJ, Garc\u00eda-Bernal D, O\u00f1ate-S\u00e1nchez RE, Ortolani-Seltenerich PS, Forner L, Moraleda JM. Evaluation of cytocompatibility of calcium silicate-based endodontic sealers and their effects on the biological responses of mesenchymal dental stem cells. Int Endod J. 2017;50:67\u201376.","journal-title":"Int Endod J"},{"key":"380_CR37","doi-asserted-by":"publisher","first-page":"467","DOI":"10.1590\/1678-775720150170","volume":"23","author":"LB Mestieri","year":"2015","unstructured":"Mestieri LB, Gomes-Corn\u00e9lio AL, Rodrigues EM, Salles LP, Bosso-Martelo R, Guerreiro-Tanomaru JM, Tanomaru-Filho M. Biocompatibility and bioactivity of calcium silicate-based endodontic sealers in human dental pulp cells. J Appl Oral Sci. 2015;23:467\u201371.","journal-title":"J Appl Oral Sci"},{"key":"380_CR38","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1111\/j.1365-2591.2011.01941.x","volume":"45","author":"MZ Scelza","year":"2012","unstructured":"Scelza MZ, Linhares AB, da Silva LE, Granjeiro JM, Alves GG. A multiparametric assay to compare the cytotoxicity of endodontic sealers with primary human osteoblasts. Int Endod J. 2012;45:12\u20138.","journal-title":"Int Endod J"},{"key":"380_CR39","doi-asserted-by":"publisher","first-page":"62","DOI":"10.1016\/j.joen.2014.09.019","volume":"41","author":"E Assmann","year":"2015","unstructured":"Assmann E, B\u00f6ttcher DE, Hoppe CB, Grecca FS, Kopper PM. Evaluation of bone tissue response to a sealer containing mineral trioxide aggregate. J Endod. 2015;41:62\u20136.","journal-title":"J Endod"},{"key":"380_CR40","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1590\/0103-6440201302115","volume":"24","author":"P Yoshino","year":"2013","unstructured":"Yoshino P, Nishiyama CK, da Silva Modena KC, Santos CF, Sipert CR. In vitro cytotoxicity of white MTA, MTA Fillapex\u00ae and Portland cement on human periodontal ligament fibroblasts. Braz Dent J. 2013;24:111\u20136.","journal-title":"Braz Dent J"},{"key":"380_CR41","doi-asserted-by":"publisher","first-page":"1290","DOI":"10.1016\/j.dental.2015.08.163","volume":"31","author":"S Dimitrova-Nakov","year":"2015","unstructured":"Dimitrova-Nakov S, Uzunoglu E, Ardila-Osorio H, Baudry A, Richard G, Kellermann O, Goldberg M. In vitro bioactivity of BioRoot\u2122 RCS, via A4 mouse pulp stem cells. Dent Mater. 2015;31:1290\u20137.","journal-title":"Dent Mater"},{"key":"380_CR42","doi-asserted-by":"publisher","first-page":"1784","DOI":"10.1016\/j.joen.2016.09.018","volume":"42","author":"R-K Pr\u00fcllage","year":"2016","unstructured":"Pr\u00fcllage R-K, Urban K, Sch\u00e4fer E, Dammaschke T. Material Properties of a tricalcium silicate-containing, a mineral trioxide aggregate-containing, and an epoxy resin-based root canal sealer. J Endod. 2016;42:1784\u20138.","journal-title":"J Endod"}],"container-title":["Odontology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s10266-018-0380-3\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s10266-018-0380-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s10266-018-0380-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,3]],"date-time":"2023-09-03T22:27:08Z","timestamp":1693780028000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s10266-018-0380-3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,7,23]]},"references-count":42,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,1]]}},"alternative-id":["380"],"URL":"https:\/\/doi.org\/10.1007\/s10266-018-0380-3","relation":{},"ISSN":["1618-1247","1618-1255"],"issn-type":[{"value":"1618-1247","type":"print"},{"value":"1618-1255","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,7,23]]},"assertion":[{"value":"24 April 2018","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"21 June 2018","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 July 2018","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with ethical standards"}},{"value":"The authors declare that they have no conflict of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}]}}