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Early detection of biomarkers associated with periodontal disease (PD) can significantly aid in timely diagnosis and intervention. Myeloperoxidase (MPO) is an enzyme abundantly present in neutrophils and has been associated in the pathogenesis of PD. Here, we present a novel approach for the electrochemical detection of MPO using printed screen graphene electrodes coupled with principal component analysis (PCA) for data analysis. We employed cyclic voltammetry to characterize the electrochemical behavior of MPO using potassium ferrocyanide and hydrogen peroxide. The process was controlled by species diffusion on the electrode surface using a scan rate spanning from 10 to 400\u00a0mVs<jats:sup>\u22121<\/jats:sup>. In addition, we investigated the detection of hydrogen peroxide, a substrate of MPO, as a method to indirectly asses MPO electroactivity, leveraging a redox potential of \u2212 500\u00a0mV. Saliva samples were collected and analyzed using the developed electrochemical sensor, followed by principal component analysis to differentiate between healthy and diseased samples based on MPO levels. Our findings demonstrate the feasibility of using printed screen graphene electrodes for the sensitive and selective detection of MPO, offering a promising approach for early diagnosis and monitoring of periodontal disease. In conclusion, our results highlight the potential of MPO as a robust biomarker for periodontal disease and highlight the utility of electrochemical sensing coupled with PCA analysis for sensitive and specific detection in clinical settings.<\/jats:p>","DOI":"10.1007\/s10266-024-01043-8","type":"journal-article","created":{"date-parts":[[2025,2,15]],"date-time":"2025-02-15T12:36:32Z","timestamp":1739622992000},"page":"1053-1061","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Myeloperoxidase as a biomarker in periodontal disease: electrochemical detection using printed screen graphene electrodes"],"prefix":"10.1007","volume":"113","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-2618-6470","authenticated-orcid":false,"given":"Mar\u00eda C.","family":"Valdivieso","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8874-9427","authenticated-orcid":false,"given":"Ludy","family":"Ortiz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6751-2305","authenticated-orcid":false,"given":"John J.","family":"Castillo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,2,15]]},"reference":[{"key":"1043_CR1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cden.2005.03.006","author":"HC Tenenbaum","year":"2005","unstructured":"Tenenbaum HC, Tenenbaum H, Zohar R. 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