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A combination of vitamins C and A at specified concentrations enhanced cellular dedifferentiation and multipotency of hGMSCs. To our knowledge, vitamins A\/C-primed hGMSCs in the prevention of medication-related osteonecrosis of the jaw (MRONJ) have not been investigated. Thus, this study evaluated the impact of vitamins A\/C in pluripotency-inductive concentrations on the regenerative\/reparative potential of hGMSCs in preventing MRONJ development in rats. Thirty albino rats (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200910\/group) were administered zoledronic acid and dexamethasone three times a week for 4\u00a0weeks. The upper first molar was extracted 2\u00a0weeks following the first administration. Immediately after extraction, rats received normal saline (group \u0399), hGMSCs (group \u0399\u0399), and hGMSCs primed with vitamins A\/C (group \u0399\u0399\u0399). Rats were euthanized 2 and 4\u00a0weeks after extraction. The maxillae were analyzed using dual-energy X-ray absorptiometry, histologically, histomorphometrically, and by quantitative real-time polymerase chain reaction analysis. Groups II and III displayed elevated bone mineral density, with a significant rise in group \u0399\u0399\u0399 relative to group \u0399\u0399 at 4 weeks. Histomorphometrically, groups II and III revealed an elevation in area percentage of newly generated bone, with a significant rise in group \u0399\u0399\u0399 at both time intervals. Gene expression of transforming growth factor-\u03b21 (TGF-\u03b21) and runt-related transcription factor 2 (Runx2) presented a significant elevation in groups II and III, with a significant rise in group \u0399\u0399\u0399 at both time intervals. In conclusion, vitamins A\/C-primed hGMSCs at the tested pluripotency-inductive concentrations prevented MRONJ development, primarily through encouraging wound healing and bone repair in the extraction sockets of rats.\n                  <\/jats:p>","DOI":"10.1007\/s10266-025-01288-x","type":"journal-article","created":{"date-parts":[[2026,1,4]],"date-time":"2026-01-04T03:09:34Z","timestamp":1767496174000},"page":"2214-2225","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Effect of vitamins A\/C primed human gingival mesenchymal stem\/progenitor cells in preventing medication-related osteonecrosis of extraction socket: an animal study"],"prefix":"10.1007","volume":"114","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-4437-2916","authenticated-orcid":false,"given":"Fatma I.","family":"Elfaiedi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7989-0733","authenticated-orcid":false,"given":"Nahed S.","family":"Korany","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6261-3609","authenticated-orcid":false,"given":"Karim","family":"Fawzy El-Sayed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2962-0070","authenticated-orcid":false,"given":"Nermeen","family":"AbuBakr","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,1,4]]},"reference":[{"key":"1288_CR1","doi-asserted-by":"publisher","first-page":"489","DOI":"10.1016\/j.coms.2015.06.001","volume":"27","author":"T Aghaloo","year":"2015","unstructured":"Aghaloo T, Hazboun R, Tetradis S. 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