{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T21:57:14Z","timestamp":1790891834492,"version":"4.1.0"},"reference-count":45,"publisher":"Springer Science and Business Media LLC","issue":"4","license":[{"start":{"date-parts":[[2026,1,11]],"date-time":"2026-01-11T00:00:00Z","timestamp":1768089600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,1,11]],"date-time":"2026-01-11T00:00:00Z","timestamp":1768089600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Odontology"],"published-print":{"date-parts":[[2026,10]]},"DOI":"10.1007\/s10266-026-01309-3","type":"journal-article","created":{"date-parts":[[2026,1,11]],"date-time":"2026-01-11T16:11:32Z","timestamp":1768147892000},"page":"2455-2463","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effect of Bio-C Temp calcium silicate-based intracanal medication on the chemical composition and fracture resistance of root dentin"],"prefix":"10.1007","volume":"114","author":[{"given":"Andriele Pache","family":"Ferigolo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmela Manara","family":"Vier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura Fagundes","family":"Dalforno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duvan Cala","family":"Castillo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana Rosemara Felisberto","family":"da Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7524-0370","authenticated-orcid":false,"given":"Guilherme","family":"Pauletto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,1,11]]},"reference":[{"key":"1309_CR1","doi-asserted-by":"publisher","first-page":"1545","DOI":"10.1016\/j.joen.2020.08.002","volume":"46","author":"NR Bedran","year":"2020","unstructured":"Bedran NR, Nadelman P, Magno MB, de Almeida Neves A, Ferreira DM, Braga Pintor AV, et al. Does calcium hydroxide reduce endotoxins in infected root canals? Systematic review and meta-analysis. J Endod. 2020;46:1545\u201358.","journal-title":"J Endod"},{"key":"1309_CR2","doi-asserted-by":"publisher","first-page":"613","DOI":"10.1111\/iej.13731","volume":"55","author":"R Ordinola-Zapata","year":"2022","unstructured":"Ordinola-Zapata R, Noblett WC, Perez-Ron A, Ye Z, Vera J. Present status and future directions of intracanal medicaments. Int Endod J. 2022;55:613\u201336.","journal-title":"Int Endod J"},{"key":"1309_CR3","doi-asserted-by":"publisher","first-page":"1327","DOI":"10.1016\/j.joen.2011.05.033","volume":"37","author":"ZC Cehreli","year":"2011","unstructured":"Cehreli ZC, Isbitiren B, Sara S, Erbas G. Regenerative endodontic treatment (revascularization) of immature necrotic molars medicated with calcium hydroxide: a case series. J Endod. 2011;37:1327\u201330.","journal-title":"J Endod"},{"key":"1309_CR4","first-page":"29","volume":"10","author":"F Fonzar","year":"2017","unstructured":"Fonzar F, Mollo A, Venturi M, Pini P, Fabian Fonzar R, Trullenque-Eriksson A, et al. Single versus two visits with 1-week intracanal calcium hydroxide medication for endodontic treatment: One-year post-treatment results from a multicentre randomised controlled trial. Eur J Oral Implantol. 2017;10:29\u201341.","journal-title":"Eur J Oral Implantol"},{"key":"1309_CR5","doi-asserted-by":"publisher","first-page":"833","DOI":"10.1111\/iej.13758","volume":"55","author":"F Karao\u011flan","year":"2022","unstructured":"Karao\u011flan F, Mi\u00e7oo\u011fullar\u0131 Kurt S, \u00c7al\u0131\u015fkan MK. Outcome of single- versus two-visit root canal retreatment in teeth with periapical lesions: a randomized clinical trial. Int Endod J. 2022;55:833\u201343.","journal-title":"Int Endod J"},{"key":"1309_CR6","doi-asserted-by":"publisher","first-page":"735","DOI":"10.1038\/sj.bdj.4811897","volume":"197","author":"P Carrotte","year":"2004","unstructured":"Carrotte P. Endodontics: Part 9. Calcium hydroxide, root resorption, endo-perio lesions. Br Dent J. 2004;197:735\u201343.","journal-title":"Br Dent J"},{"key":"1309_CR7","doi-asserted-by":"publisher","first-page":"1417","DOI":"10.1016\/j.joen.2017.03.033","volume":"43","author":"NA Taha","year":"2017","unstructured":"Taha NA, Khazali MA. Partial pulpotomy in mature permanent teeth with clinical signs indicative of irreversible pulpitis: a randomized clinical trial. J Endod. 2017;43:1417\u201321.","journal-title":"J Endod"},{"key":"1309_CR8","doi-asserted-by":"publisher","first-page":"5079","DOI":"10.1007\/s00784-022-04481-2","volume":"26","author":"H Lu","year":"2022","unstructured":"Lu H, Lu J, Guo J, Zeng B, Zeng Q, Zhao W, et al. Radiographic outcomes and prognostic factors in nonvital immature permanent teeth after apexification with modified calcium hydroxide paste: a retrospective study. Clin Oral Investig. 2022;26:5079\u201388.","journal-title":"Clin Oral Investig"},{"key":"1309_CR9","doi-asserted-by":"publisher","first-page":"293","DOI":"10.1111\/j.1875-595X.2005.tb00326.x","volume":"55","author":"A Farhad","year":"2005","unstructured":"Farhad A, Mohammadi Z. Calcium hydroxide: a review. Int Dent J. 2005;55:293\u2013301.","journal-title":"Int Dent J"},{"key":"1309_CR10","doi-asserted-by":"publisher","first-page":"1714","DOI":"10.1016\/j.joen.2024.08.005","volume":"50","author":"C Sunlakawit","year":"2024","unstructured":"Sunlakawit C, Chaimanakarn C, Srimaneekarn N, Osiri S. Effect of calcium hydroxide as an intracanal medication on dentine fracture resistance: a systematic review and network meta-analysis. J Endod. 2024;50:1714\u201324.","journal-title":"J Endod"},{"key":"1309_CR11","doi-asserted-by":"publisher","first-page":"697","DOI":"10.1111\/j.1365-2591.2011.01886.x","volume":"44","author":"Z Mohammadi","year":"2011","unstructured":"Mohammadi Z, Dummer PM. Properties and applications of calcium hydroxide in endodontics and dental traumatology. Int Endod J. 2011;44:697\u2013730.","journal-title":"Int Endod J"},{"key":"1309_CR12","doi-asserted-by":"publisher","DOI":"10.3390\/ma10121412","volume":"10","author":"S Shetty","year":"2017","unstructured":"Shetty S, Kahler SL, Kahler B. Alkaline material effects on roots of teeth. Materials. 2017;10:1412.","journal-title":"Materials"},{"key":"1309_CR13","doi-asserted-by":"publisher","first-page":"618","DOI":"10.1111\/edt.12979","volume":"40","author":"B Kahler","year":"2024","unstructured":"Kahler B, Lu J, Taha NA. Regenerative endodontic treatment and traumatic dental injuries. Dent Traumatol. 2024;40:618\u201335.","journal-title":"Dent Traumatol"},{"key":"1309_CR14","doi-asserted-by":"publisher","first-page":"598","DOI":"10.1590\/0103-6440202003376","volume":"31","author":"N Villa","year":"2020","unstructured":"Villa N, Santos VVD, Costa UMD, Mendes AT, Duarte PHM, Rosa RAD, et al. A new calcium silicate-based root canal dressing: physical and chemical properties, cytotoxicity and dentinal tubule penetration. Braz Dent J. 2020;31:598\u2013604.","journal-title":"Braz Dent J"},{"key":"1309_CR15","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdent.2024.105311","volume":"149","author":"LP de Ara\u00fajo","year":"2024","unstructured":"de Ara\u00fajo LP, Immich F, da Rosa WLO, da Silva AF, Lund RG, Piva E. Current perspectives on calcium silicate-based intracanal medicaments: a scoping review of clinical and laboratory evidence. J Dent. 2024;149:105311.","journal-title":"J Dent"},{"key":"1309_CR16","doi-asserted-by":"publisher","first-page":"1140","DOI":"10.1111\/iej.13308","volume":"53","author":"LV Oliveira","year":"2020","unstructured":"Oliveira LV, da Silva GR, Souza GL, Magalh\u00e3es TEA, Barbosa GLR, Turrioni AP, et al. A laboratory evaluation of cell viability, radiopacity and tooth discoloration induced by regenerative endodontic materials. Int Endod J. 2020;53:1140\u201352.","journal-title":"Int Endod J"},{"key":"1309_CR17","doi-asserted-by":"publisher","first-page":"2133","DOI":"10.1111\/iej.13616","volume":"54","author":"LV Oliveira","year":"2021","unstructured":"Oliveira LV, de Souza GL, da Silva GR, Magalh\u00e3es TEA, Freitas GAN, Turrioni AP, et al. Biological parameters, discolouration and radiopacity of calcium silicate-based materials in a simulated model of partial pulpotomy. Int Endod J. 2021;54:2133\u201344.","journal-title":"Int Endod J"},{"key":"1309_CR18","doi-asserted-by":"publisher","first-page":"1155","DOI":"10.1111\/iej.13502","volume":"54","author":"JCM Guerreiro","year":"2021","unstructured":"Guerreiro JCM, Ochoa-Rodr\u00edgez VM, Rodrigues EM, Chavez-Andrade GM, Tanomaru-Filho M, Guerreiro-Tanomaru JM, et al. Antibacterial activity, cytocompatibility and effect of Bio-C Temp bioceramic intracanal medicament on osteoblast biology. Int Endod J. 2021;54:1155\u201365.","journal-title":"Int Endod J"},{"key":"1309_CR19","doi-asserted-by":"publisher","first-page":"54","DOI":"10.1590\/0103-6440202305506","volume":"34","author":"BL Capitanio","year":"2023","unstructured":"Capitanio BL, Hashizume LN, Kuga MC, Oliveira ECG, Rosa RAD, S\u00f3 GB, et al. Analysis of pH, calcium ion release, and energy dispersive spectroscopy of a bioceramic root canal dressing. Braz Dent J. 2023;34:54\u201361.","journal-title":"Braz Dent J"},{"key":"1309_CR20","doi-asserted-by":"publisher","DOI":"10.1016\/j.pdpdt.2024.104088","volume":"47","author":"A Alshamrani","year":"2024","unstructured":"Alshamrani A, AlDeeb L, Almohareb T, Alahdal K, Maawadh A, Alrahlah A. Effect of canal medicaments triple antibiotic paste, Bio-C Temp, and Nano-silver gel activated by visible blue light on canal dentin microhardness and extrusion bond strength of AH plus sealer: a SEM and EDX analysis. Photodiagn Photodyn Ther. 2024;47:104088.","journal-title":"Photodiagn Photodyn Ther"},{"key":"1309_CR21","doi-asserted-by":"publisher","DOI":"10.1111\/eos.12798","volume":"129","author":"AS Al-Hiyasat","year":"2021","unstructured":"Al-Hiyasat AS, El-Farraj HS, Alebrahim MA. The effect of calcium hydroxide on dentine composition and root fracture resistance of human teeth: an in vitro study. Eur J Oral Sci. 2021;129:e12798.","journal-title":"Eur J Oral Sci"},{"key":"1309_CR22","doi-asserted-by":"publisher","first-page":"1843","DOI":"10.1016\/j.joen.2018.08.011","volume":"44","author":"S Osiri","year":"2018","unstructured":"Osiri S, Banomyong D, Sattabanasuk V, Yanpiset K. Root reinforcement after obturation with calcium silicate-based sealer and modified gutta-percha cone. J Endod. 2018;44:1843\u20138.","journal-title":"J Endod"},{"key":"1309_CR23","doi-asserted-by":"crossref","unstructured":"Pauletto G, de Pellegrin SF, Padoin Y, Vargas AW, Castillo DC, Pereira GKR, et al. Influence of apical root resection level and filling technique on the fracture resistance of endodontically treated teeth: a biomechanical study. Odontology. 2025.","DOI":"10.1007\/s10266-025-01185-3"},{"key":"1309_CR24","doi-asserted-by":"publisher","first-page":"105","DOI":"10.15171\/jlms.2016.18","volume":"7","author":"A Ghorbanzadeh","year":"2016","unstructured":"Ghorbanzadeh A, Aminsobhani M, Sohrabi K, Chiniforush N, Ghafari S, Shamshiri AR, et al. Penetration depth of sodium hypochlorite in dentinal tubules after conventional irrigation, passive ultrasonic agitation and Nd:YAG laser activated irrigation. J Lasers Med Sci. 2016;7:105\u201311.","journal-title":"J Lasers Med Sci"},{"key":"1309_CR25","doi-asserted-by":"publisher","first-page":"408","DOI":"10.1002\/jemt.22992","volume":"81","author":"PHM Duarte","year":"2018","unstructured":"Duarte PHM, da Silva PB, Rosa RAD, Montagner F, Duarte MAH, Kuga MC, et al. Effect of ethanol on the antimicrobial properties of chlorhexidine over oral biofilm. Microsc Res Tech. 2018;81:408\u201312.","journal-title":"Microsc Res Tech"},{"key":"1309_CR26","doi-asserted-by":"publisher","first-page":"261","DOI":"10.1007\/s10266-024-00971-9","volume":"113","author":"G Pauletto","year":"2025","unstructured":"Pauletto G, Machry RV, Baumhardt T, de Freitas Daudt N, Pereira GKR, Bier CAS. Effect of radiotherapy, immediate dentin sealing and irrigation simulating single- or two-visits endodontic treatment on the bond strength to pulp chamber dentin: an in vitro study. Odontology. 2025;113:261\u201373.","journal-title":"Odontology"},{"key":"1309_CR27","doi-asserted-by":"publisher","first-page":"895","DOI":"10.1097\/01.don.0000194542.02521.af","volume":"31","author":"GE Doyon","year":"2005","unstructured":"Doyon GE, Dumsha T, von Fraunhofer JA. Fracture resistance of human root dentin exposed to intracanal calcium hydroxide. J Endod. 2005;31:895\u20137.","journal-title":"J Endod"},{"key":"1309_CR28","doi-asserted-by":"publisher","first-page":"269","DOI":"10.1016\/j.joen.2012.09.020","volume":"39","author":"GH Yassen","year":"2013","unstructured":"Yassen GH, Chu TM, Eckert G, Platt JA. Effect of medicaments used in endodontic regeneration technique on the chemical structure of human immature radicular dentin: an in vitro study. J Endod. 2013;39:269\u201373.","journal-title":"J Endod"},{"key":"1309_CR29","first-page":"67","volume":"69","author":"JB Hoffmann","year":"2021","unstructured":"Hoffmann JB, Vitali FC, Hillesheim LC, Tedesco M, Garcia LFR, Bortoluzzi EA, et al. Effect of different periods of calcium hydroxide dressing on the fracture resistance of root dentin. Gen Dent. 2021;69:67\u201371.","journal-title":"Gen Dent"},{"key":"1309_CR30","doi-asserted-by":"publisher","DOI":"10.1590\/1807-3107bor-2025.vol39.029","volume":"39","author":"PC Siqueira","year":"2025","unstructured":"Siqueira PC, Soares LDCC, Wanderley KRDM, Deus LB, Melo HV, \u00c1vila MPA, et al. The impact of intracanal medicaments on crown color, pH, and antimicrobial activity: a comparative study. Braz Oral Res. 2025;39:e029.","journal-title":"Braz Oral Res"},{"key":"1309_CR31","doi-asserted-by":"publisher","first-page":"1437","DOI":"10.1016\/j.joen.2011.06.005","volume":"37","author":"W Qian","year":"2011","unstructured":"Qian W, Shen Y, Haapasalo M. Quantitative analysis of the effect of irrigant solution sequences on dentin erosion. J Endod. 2011;37:1437\u201341.","journal-title":"J Endod"},{"key":"1309_CR32","doi-asserted-by":"publisher","first-page":"1655","DOI":"10.1016\/j.dental.2020.10.010","volume":"36","author":"PP Rath","year":"2020","unstructured":"Rath PP, Yiu CKY, Matinlinna JP, Kishen A, Neelakantan P. The effects of sequential and continuous chelation on dentin. Dent Mater. 2020;36:1655\u201365.","journal-title":"Dent Mater"},{"key":"1309_CR33","doi-asserted-by":"publisher","DOI":"10.3389\/fdmed.2024.1451275","volume":"5","author":"C El Hachem","year":"2024","unstructured":"El Hachem C, Sauro S, Eid A, Ar\u0131can B, Alrayesse R, Fabro C, et al. Novel temporary endodontic medication based on calcium silicate strategy: a biological and physicochemical study. Front Dental Med. 2024;5:1451275.","journal-title":"Front Dental Med"},{"issue":"1","key":"1309_CR34","doi-asserted-by":"publisher","DOI":"10.1007\/s10856-024-06781-3","volume":"35","author":"CS Lopes","year":"2024","unstructured":"Lopes CS, Delfino MM, Tanomaru-Filho M, Sasso-Cerri E, Guerreiro-Tanomaru JM, Cerri PS. Bioactive potential of Bio-C Temp demonstrated by systemic mineralization markers and immunoexpression of bone proteins in the rat connective tissue. J Mater Sci Mater Med. 2024;35(1):13.","journal-title":"J Mater Sci Mater Med"},{"key":"1309_CR35","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmbbm.2020.103736","volume":"106","author":"M Durigon","year":"2020","unstructured":"Durigon M, Cecchin D, de Carli JP, Souza MA, Farina AP. Could calcium hypochlorite and grape seed extract keep the mechanical properties of root dentin and fracture resistance of weakened roots? J Mech Behav Biomed Mater. 2020;106:103736.","journal-title":"J Mech Behav Biomed Mater"},{"issue":"1","key":"1309_CR36","doi-asserted-by":"publisher","DOI":"10.1186\/s12903-024-03954-y","volume":"24","author":"RM Barakat","year":"2024","unstructured":"Barakat RM, Almohareb RA, Alsuwaidan M, Faqehi E, Alaidarous E, Algahtani FN. Effect of sodium hypochlorite temperature and concentration on the fracture resistance of root dentin. BMC Oral Health. 2024;24(1):233.","journal-title":"BMC Oral Health"},{"key":"1309_CR37","doi-asserted-by":"publisher","first-page":"142","DOI":"10.1007\/s11596-013-1087-x","volume":"33","author":"HY Khaleel","year":"2013","unstructured":"Khaleel HY, Al-Ashaw AJ, Yang Y, Pang AH, Ma JZ. Quantitative comparison of calcium hydroxide removal by EndoActivator, ultrasonic and ProTaper file agitation techniques: an in vitro study. J Huazhong Univ Sci Technol Med Sci. 2013;33:142\u20135.","journal-title":"J Huazhong Univ Sci Technol Med Sci"},{"issue":"6","key":"1309_CR38","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0268791","volume":"17","author":"W Moon","year":"2022","unstructured":"Moon W, Chung SH, Chang J. Sonic irrigation for removal of calcium hydroxide in the apical root canal: a micro-CT and light-coupled tracking analysis. PLoS ONE. 2022;17(6):e0268791.","journal-title":"PLoS ONE"},{"key":"1309_CR39","doi-asserted-by":"publisher","first-page":"1053","DOI":"10.1016\/j.joen.2025.05.013","volume":"51","author":"JG Edwards","year":"2025","unstructured":"Edwards JG, Nusstein J, Drum M, Fowler S, Reader A. Micro-computed tomographic analysis of the efficacy of 3 techniques for removal of calcium hydroxide from the mesial canals of curved mandibular molars. J Endod. 2025;51:1053\u201360.","journal-title":"J Endod"},{"key":"1309_CR40","doi-asserted-by":"publisher","first-page":"1600","DOI":"10.1016\/j.joen.2014.02.028","volume":"40","author":"EM Souza","year":"2014","unstructured":"Souza EM, Calixto AM, Lima CN, Pappen FG, De-Deus G. Similar influence of stabilized alkaline and neutral sodium hypochlorite solutions on the fracture resistance of root canal-treated bovine teeth. J Endod. 2014;40:1600\u20133.","journal-title":"J Endod"},{"key":"1309_CR41","doi-asserted-by":"publisher","first-page":"385","DOI":"10.1111\/edt.12185","volume":"31","author":"MC Valera","year":"2015","unstructured":"Valera MC, Albuquerque MT, Yamasaki MC, Vassallo FN, da Silva DA, Nagata JY. Fracture resistance of weakened bovine teeth after long-term use of calcium hydroxide. Dent Traumatol. 2015;31:385\u20139.","journal-title":"Dent Traumatol"},{"key":"1309_CR42","doi-asserted-by":"publisher","first-page":"1725","DOI":"10.1007\/s00784-021-04148-4","volume":"26","author":"TDSA Santos","year":"2022","unstructured":"Santos TDSA, Abu Hasna A, Abreu RT, Tribst JPM, de Andrade GS, Borges ALS, et al. Fracture resistance and stress distribution of weakened teeth reinforced with a bundled glass fiber-reinforced resin post. Clin Oral Investig. 2022;26:1725\u201335.","journal-title":"Clin Oral Investig"},{"key":"1309_CR43","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1111\/adj.12655","volume":"64","author":"S Cochrane","year":"2019","unstructured":"Cochrane S, Burrow MF, Parashos P. Effect on the mechanical properties of human and bovine dentine of intracanal medicaments and irrigants. Aust Dent J. 2019;64:35\u201342.","journal-title":"Aust Dent J"},{"key":"1309_CR44","doi-asserted-by":"publisher","DOI":"10.3390\/ma16041514","volume":"16","author":"S M\u00f6hring","year":"2023","unstructured":"M\u00f6hring S, Cieplik F, Hiller KA, Ebensberger H, Ferstl G, Hermens J, et al. Elemental compositions of enamel or dentin in human and bovine teeth differ from murine teeth. Materials. 2023;16:1514.","journal-title":"Materials"},{"key":"1309_CR45","doi-asserted-by":"crossref","unstructured":"Al Ankily M, Baz S, Mahmoud H, Bakr MM, Shamel M. Bovine teeth as substitutes for human teeth in dental research: ultrastructural and radiographic analysis. Eur J Dent. 2025.","DOI":"10.21203\/rs.3.rs-5434854\/v1"}],"container-title":["Odontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10266-026-01309-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10266-026-01309-3","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10266-026-01309-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T21:15:56Z","timestamp":1790889356000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10266-026-01309-3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,11]]},"references-count":45,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["1309"],"URL":"https:\/\/doi.org\/10.1007\/s10266-026-01309-3","relation":{},"ISSN":["1618-1247","1618-1255"],"issn-type":[{"value":"1618-1247","type":"print"},{"value":"1618-1255","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,11]]},"assertion":[{"value":"17 October 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"4 January 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"11 January 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","label":"Conflict of interest","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The study was not submitted to a research ethics committee because the samples consisted of discarded bovine teeth obtained after slaughter for commercial purposes. No animals were killed specifically for this research, and all applicable international, national, and\/or institutional guidelines for the ethical use of animal derived materials were followed.","order":2,"name":"Ethics","label":"Ethical approval","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"For this type of study, formal consent is not required.","order":3,"name":"Ethics","label":"Informed consent","group":{"name":"EthicsHeading","label":"Declarations"}}]}}