{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T21:39:29Z","timestamp":1792532369841,"version":"4.5.0"},"reference-count":62,"publisher":"Springer Science and Business Media LLC","license":[{"start":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T00:00:00Z","timestamp":1791072000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T00:00:00Z","timestamp":1791072000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Odontology"],"DOI":"10.1007\/s10266-026-01566-2","type":"journal-article","created":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T21:30:38Z","timestamp":1791149438000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparison of the effects of different dental pulp stem cell preconditioning protocols on the odontogenic potential of their derived small extracellular vesicles"],"prefix":"10.1007","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8577-4301","authenticated-orcid":false,"given":"Rana","family":"El-Qashty","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4391-9725","authenticated-orcid":false,"given":"Ahmed A.","family":"Emam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8830-8100","authenticated-orcid":false,"given":"Mona H.","family":"El-Zekrid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4875-7534","authenticated-orcid":false,"given":"Osama A.","family":"Elkashty","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9993-1264","authenticated-orcid":false,"given":"Tarek Mohamed","family":"Abdelwahab","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4978-0987","authenticated-orcid":false,"given":"Noha Tharwat","family":"Abou El-Khier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6606-2103","authenticated-orcid":false,"given":"Eman Gamal","family":"Sobh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0816-1928","authenticated-orcid":false,"given":"Eman","family":"Hany","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,10,4]]},"reference":[{"issue":"4","key":"1566_CR1","first-page":"258","volume":"22","author":"OO Olatosi","year":"2012","unstructured":"Olatosi OO, Sote EO. Causes and pattern of tooth loss in children and adolescents in a Nigerian tertiary hospital. Nig Q J Hosp Med. 2012;22(4):258\u201362.","journal-title":"Nig Q J Hosp Med"},{"issue":"1","key":"1566_CR2","first-page":"15","volume":"9","author":"J Ghoddusi","year":"2014","unstructured":"Ghoddusi J, Forghani M, Parisay I. New approaches in vital pulp therapy in permanent teeth. Iran Endod J. 2014;9(1):15\u201322.","journal-title":"Iran Endod J"},{"key":"1566_CR3","doi-asserted-by":"publisher","unstructured":"Sonia EL, Cahyani C, editors. The Effect of Odontoblast Formation on Reversible Pulpitis After Application of Calcium Hydroxide and Mineral Trioxide Aggregate: Literature Review. International Conference on Health and Well-Being (ICHWB 2022); 2023: Atlantis Press. https:\/\/doi.org\/10.2991\/978-94-6463-184-5_7","DOI":"10.2991\/978-94-6463-184-5_7"},{"issue":"1","key":"1566_CR4","doi-asserted-by":"publisher","first-page":"466","DOI":"10.3390\/ijms24010466","volume":"24","author":"V Ganesh","year":"2023","unstructured":"Ganesh V, Seol D, Gomez-Contreras PC, Keen HL, Shin K, Martin JA. Exosome-Based Cell Homing and Angiogenic Differentiation for Dental Pulp Regeneration. Int J Mol Sci. 2023;24(1):466. https:\/\/doi.org\/10.3390\/ijms24010466.","journal-title":"Int J Mol Sci"},{"key":"1566_CR5","doi-asserted-by":"publisher","first-page":"3246811","DOI":"10.1155\/2022\/3246811","volume":"2022","author":"V Khampatee","year":"2022","unstructured":"Khampatee V, Zhang C, Chou L. Effects of Aspirin on Odontogenesis of Human Dental Pulp Cells and TGF-\u03b21 Liberation from Dentin In Vitro. Int J Dent. 2022;2022:3246811. https:\/\/doi.org\/10.1155\/2022\/3246811.","journal-title":"Int J Dent"},{"issue":"12","key":"1566_CR6","doi-asserted-by":"publisher","first-page":"956","DOI":"10.1016\/j.jfma.2014.09.003","volume":"113","author":"CC Chang","year":"2014","unstructured":"Chang CC, Chang KC, Tsai SJ, Chang HH, Lin CP. Neurogenic differentiation of dental pulp stem cells to neuron-like cells in dopaminergic and motor neuronal inductive media. J Formos Med Assoc. 2014;113(12):956\u201365. https:\/\/doi.org\/10.1016\/j.jfma.2014.09.003.","journal-title":"J Formos Med Assoc"},{"issue":"6","key":"1566_CR7","doi-asserted-by":"publisher","first-page":"1576","DOI":"10.1002\/stem.2334","volume":"34","author":"Z Zhang","year":"2016","unstructured":"Zhang Z, N\u00f6r F, Oh M, Cucco C, Shi S, N\u00f6r JE. Wnt\/\u03b2-Catenin Signaling Determines the Vasculogenic Fate of Postnatal Mesenchymal Stem Cells. Stem Cells. 2016;34(6):1576\u201387. https:\/\/doi.org\/10.1002\/stem.2334.","journal-title":"Stem Cells"},{"key":"1566_CR8","doi-asserted-by":"publisher","first-page":"1174579","DOI":"10.3389\/fcell.2023.1174579","volume":"11","author":"H Pan","year":"2023","unstructured":"Pan H, Yang Y, Xu H, Jin A, Huang X, Gao X, et al. The odontoblastic differentiation of dental mesenchymal stem cells: molecular regulation mechanism and related genetic syndromes. Front Cell Dev Biology. 2023;11:1174579. https:\/\/doi.org\/10.3389\/fcell.2023.1174579.","journal-title":"Front Cell Dev Biology"},{"issue":"1","key":"1566_CR9","doi-asserted-by":"publisher","first-page":"439","DOI":"10.1186\/s13287-022-03124-3","volume":"13","author":"S Ziauddin","year":"2022","unstructured":"Ziauddin S, Nakashima M, Watanabe H, Tominaga M, Iohara K. Biological characteristics and pulp regeneration potential of stem cells from canine deciduous teeth compared with those of permanent teeth. Stem Cell Res Ther. 2022;13(1):439. https:\/\/doi.org\/10.1186\/s13287-022-03124-3.","journal-title":"Stem Cell Res Ther"},{"issue":"1","key":"1566_CR10","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1089\/teb.2007.0216","volume":"14","author":"DM Choumerianou","year":"2008","unstructured":"Choumerianou DM, Dimitriou H, Kalmanti M. Stem cells: promises versus limitations. Tissue Eng Part B Rev. 2008;14(1):53\u201360. https:\/\/doi.org\/10.1089\/teb.2007.0216.","journal-title":"Tissue Eng Part B Rev"},{"issue":"1","key":"1566_CR11","doi-asserted-by":"publisher","first-page":"2616807","DOI":"10.1155\/2021\/2616807","volume":"2021","author":"JB Foo","year":"2021","unstructured":"Foo JB, Looi QH, Chong PP, Hassan NH, Yeo GEC, Ng CY, et al. Comparing the therapeutic potential of stem cells and their secretory products in regenerative medicine. Stem cells Int. 2021;2021(1):2616807. https:\/\/doi.org\/10.1155\/2021\/2616807.","journal-title":"Stem cells Int"},{"issue":"1","key":"1566_CR12","doi-asserted-by":"publisher","first-page":"720","DOI":"10.1186\/s12903-023-03466-1","volume":"23","author":"R El-Qashty","year":"2023","unstructured":"El-Qashty R, Elkashty OA, Hany E. Photobiostimulation conjugated with stem cells or their secretome for temporomandibular joint arthritis in a rat model. BMC Oral Health. 2023;23(1):720. https:\/\/doi.org\/10.1186\/s12903-023-03466-1.","journal-title":"BMC Oral Health"},{"issue":"1","key":"1566_CR13","doi-asserted-by":"publisher","first-page":"253","DOI":"10.21608\/ejh.2022.175334.1818","volume":"47","author":"E Hany","year":"2024","unstructured":"Hany E, Elkashty AAR, El-Qashty R. The regenerative potential of adipose-derived stem cells versus their conditioned media on tongue defects of adult male rats. Egypt J Histol. 2024;47(1):253\u201367. https:\/\/doi.org\/10.21608\/ejh.2022.175334.1818.","journal-title":"Egypt J Histol"},{"issue":"7","key":"1566_CR14","doi-asserted-by":"publisher","first-page":"1222","DOI":"10.1016\/j.spinee.2022.01.012","volume":"22","author":"E Gonz\u00e1lez-Cubero","year":"2022","unstructured":"Gonz\u00e1lez-Cubero E, Gonz\u00e1lez-Fern\u00e1ndez ML, Olivera ER, Villar-Su\u00e1rez V. Extracellular vesicle and soluble fractions of adipose tissue-derived mesenchymal stem cells secretome induce inflammatory cytokines modulation in an in vitro model of discogenic pain. Spine J. 2022;22(7):1222\u201334. https:\/\/doi.org\/10.1016\/j.spinee.2022.01.012.","journal-title":"Spine J"},{"key":"1566_CR15","doi-asserted-by":"publisher","first-page":"849905","DOI":"10.3389\/fcell.2022.849905","volume":"10","author":"PN Avalos","year":"2022","unstructured":"Avalos PN, Forsthoefel DJ. An emerging frontier in intercellular communication: extracellular vesicles in regeneration. Front Cell Dev Biology. 2022;10:849905. https:\/\/doi.org\/10.3389\/fcell.2022.849905.","journal-title":"Front Cell Dev Biology"},{"issue":"1","key":"1566_CR16","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1007\/s10565-024-09867-4","volume":"40","author":"T Xu","year":"2024","unstructured":"Xu T, Huangfu B, He X, Huang K. Exosomes as mediators of signal transmitters in biotoxins toxicity: a comprehensive review. Cell Biol Toxicol. 2024;40(1):27. https:\/\/doi.org\/10.1007\/s10565-024-09867-4.","journal-title":"Cell Biol Toxicol"},{"key":"1566_CR17","doi-asserted-by":"publisher","unstructured":"Ivica A, Ghayor C, Zehnder M, Valdec S, Weber FE. Pulp-Derived Exosomes in a Fibrin-Based Regenerative Root Filling Material. J Clin Med. 2020;9(2). https:\/\/doi.org\/10.3390\/jcm9020491.","DOI":"10.3390\/jcm9020491"},{"key":"1566_CR18","doi-asserted-by":"publisher","DOI":"10.1016\/j.jare.2024.12.048","author":"Y Wang","year":"2025","unstructured":"Wang Y, Mao J, Wang Y, Wang R, Jiang N, Hu X, et al. Odontogenic exosomes simulating the developmental microenvironment promote complete regeneration of pulp-dentin complex in vivo. J Adv Res. 2025. https:\/\/doi.org\/10.1016\/j.jare.2024.12.048.","journal-title":"J Adv Res"},{"issue":"2","key":"1566_CR19","doi-asserted-by":"publisher","first-page":"100","DOI":"10.4252\/wjsc.v12.i2.100","volume":"12","author":"M Alvarez-Viejo","year":"2020","unstructured":"Alvarez-Viejo M. Mesenchymal stem cells from different sources and their derived exosomes: A pre-clinical perspective. World J Stem Cells. 2020;12(2):100\u20139. https:\/\/doi.org\/10.4252\/wjsc.v12.i2.100.","journal-title":"World J Stem Cells"},{"key":"1566_CR20","doi-asserted-by":"publisher","DOI":"10.1111\/prd.70055","author":"P Ahmad","year":"2026","unstructured":"Ahmad P, Wang Y, Zhang Y, Sculean A, Miron RJ. Understanding exosomes in diabetic wound healing. Periodontol 2000. 2026. https:\/\/doi.org\/10.1111\/prd.70055.","journal-title":"Periodontol 2000"},{"issue":"1","key":"1566_CR21","doi-asserted-by":"publisher","first-page":"170","DOI":"10.1186\/s13287-019-1278-x","volume":"10","author":"X Hu","year":"2019","unstructured":"Hu X, Zhong Y, Kong Y, Chen Y, Feng J, Zheng J. Lineage-specific exosomes promote the odontogenic differentiation of human dental pulp stem cells (DPSCs) through TGF\u03b21\/smads signaling pathway via transfer of microRNAs. Stem Cell Res Ther. 2019;10(1):170. https:\/\/doi.org\/10.1186\/s13287-019-1278-x.","journal-title":"Stem Cell Res Ther"},{"key":"1566_CR22","doi-asserted-by":"publisher","first-page":"679","DOI":"10.1016\/j.jconrel.2020.06.006","volume":"324","author":"WB Swanson","year":"2020","unstructured":"Swanson WB, Gong T, Zhang Z, Eberle M, Niemann D, Dong R, et al. Controlled release of odontogenic exosomes from a biodegradable vehicle mediates dentinogenesis as a novel biomimetic pulp capping therapy. J Control Release. 2020;324:679\u201394. https:\/\/doi.org\/10.1016\/j.jconrel.2020.06.006.","journal-title":"J Control Release"},{"issue":"24","key":"1566_CR23","doi-asserted-by":"publisher","first-page":"5562","DOI":"10.26355\/eurrev_201712_13993","volume":"21","author":"CT Zhu","year":"2017","unstructured":"Zhu CT, Li T, Hu YH, Zou M, Guo Q, Qu XW. Exosomes secreted by mice adipose-derived stem cells after low-level laser irradiation treatment reduce apoptosis of osteocyte induced by hypoxia. Eur Rev Med Pharmacol Sci. 2017;21(24):5562\u201370. https:\/\/doi.org\/10.26355\/eurrev_201712_13993.","journal-title":"Eur Rev Med Pharmacol Sci"},{"issue":"6","key":"1566_CR24","doi-asserted-by":"publisher","first-page":"1447","DOI":"10.1111\/php.13633","volume":"98","author":"L Gholami","year":"2022","unstructured":"Gholami L, Khorsandi K, Nooshabadi VT, Shahabi S, Jazaeri M, Esfahani H, et al. Effect of Photobiomodulation on Structure and Function of Extracellular Vesicle Secreted from Mesenchymal Stem Cells. Photochem Photobiol. 2022;98(6):1447\u201358. https:\/\/doi.org\/10.1111\/php.13633.","journal-title":"Photochem Photobiol"},{"issue":"11","key":"1566_CR25","doi-asserted-by":"publisher","first-page":"6359","DOI":"10.1016\/j.sjbs.2021.06.101","volume":"28","author":"S Bhandi","year":"2021","unstructured":"Bhandi S, Alkahtani A, Mashyakhy M, Ali Baeshen H, Mustafa M, Chohan H, et al. Study of optimal conditions for growth and osteogenic differentiation of dental pulp stem cells based on glucose and serum content. Saudi J Biol Sci. 2021;28(11):6359\u201364. https:\/\/doi.org\/10.1016\/j.sjbs.2021.06.101.","journal-title":"Saudi J Biol Sci"},{"key":"1566_CR26","doi-asserted-by":"publisher","unstructured":"Hany E, Emam AA, Elbeltagy MG, Zakaria MM, Yahia S, El-Sherbiny IM, et al. Photobiostimulation Enhances the Osteogenic Differentiation of Gingival-Derived Stem Cells Seeded on Nanocomposite Scaffolds. Regenerative Eng Translational Med. 2024;1\u201319. https:\/\/doi.org\/10.1007\/s40883-024-00350-4.","DOI":"10.1007\/s40883-024-00350-4"},{"issue":"1","key":"1566_CR27","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1007\/s10103-022-03670-5","volume":"38","author":"E Giannakopoulos","year":"2022","unstructured":"Giannakopoulos E, Katopodi A, Rallis M, Politopoulos K, Alexandratou E. The effects of low power laser light at 661 nm on wound healing in a scratch assay fibroblast model. Lasers Med Sci. 2022;38(1):27. https:\/\/doi.org\/10.1007\/s10103-022-03670-5.","journal-title":"Lasers Med Sci"},{"issue":"4","key":"1566_CR28","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1006\/meth.2001.1262","volume":"25","author":"KJ Livak","year":"2001","unstructured":"Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2001;25(4):402\u20138. https:\/\/doi.org\/10.1006\/meth.2001.1262.","journal-title":"Methods"},{"key":"1566_CR29","doi-asserted-by":"publisher","first-page":"14","DOI":"10.21037\/sci-2020-015","volume":"7","author":"E Hany","year":"2020","unstructured":"Hany E, Yahia S, Elsherbeny MF, Salama NM, Ateia IM, Abou El-Khier NT, et al. Evaluation of the osteogenic potential of rat adipose-derived stem cells with different polycaprolactone\/alginate-based nanofibrous scaffolds: an in vitro study. Stem Cell Investig. 2020;7:14. https:\/\/doi.org\/10.21037\/sci-2020-015.","journal-title":"Stem Cell Investig"},{"issue":"2","key":"1566_CR30","doi-asserted-by":"publisher","first-page":"e12404","DOI":"10.1002\/jev2.12404","volume":"13","author":"JA Welsh","year":"2024","unstructured":"Welsh JA, Goberdhan DCI, O\u2019Driscoll L, Buzas EI, Blenkiron C, Bussolati B, et al. Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches. J Extracell Vesicles. 2024;13(2):e12404. https:\/\/doi.org\/10.1002\/jev2.12404.","journal-title":"J Extracell Vesicles"},{"issue":"1","key":"1566_CR31","doi-asserted-by":"publisher","first-page":"012039","DOI":"10.1088\/1742-6596\/733\/1\/012039","volume":"733","author":"TGF Souza","year":"2016","unstructured":"Souza TGF, Ciminelli VST, Mohallem NDS. A comparison of TEM and DLS methods to characterize size distribution of ceramic nanoparticles. J Phys: Conf Ser. 2016;733(1):012039. https:\/\/doi.org\/10.1088\/1742-6596\/733\/1\/012039.","journal-title":"J Phys: Conf Ser"},{"issue":"12","key":"1566_CR32","doi-asserted-by":"publisher","first-page":"5354","DOI":"10.1039\/D3MH00717K","volume":"10","author":"SK Filippov","year":"2023","unstructured":"Filippov SK, Khusnutdinov R, Murmiliuk A, Inam W, Zakharova LY, Zhang H, et al. Dynamic light scattering and transmission electron microscopy in drug delivery: a roadmap for correct characterization of nanoparticles and interpretation of results. Mater Horiz. 2023;10(12):5354\u201370. https:\/\/doi.org\/10.1039\/D3MH00717K.","journal-title":"Mater Horiz"},{"key":"1566_CR33","doi-asserted-by":"publisher","first-page":"1064412","DOI":"10.3389\/fpls.2022.1064412","volume":"13","author":"J Jang","year":"2023","unstructured":"Jang J, Jeong H, Jang E, Kim E, Yoon Y, Jang S, et al. Isolation of high-purity and high-stability exosomes from ginseng. Front Plant Sci. 2023;13:1064412. https:\/\/doi.org\/10.3389\/fpls.2022.1064412.","journal-title":"Front Plant Sci"},{"issue":"4","key":"1566_CR34","doi-asserted-by":"publisher","first-page":"851","DOI":"10.1002\/stem.2575","volume":"35","author":"DG Phinney","year":"2017","unstructured":"Phinney DG, Pittenger MF, Concise Review. MSC-Derived Exosomes for Cell-Free Therapy. Stem Cells. 2017;35(4):851\u20138. https:\/\/doi.org\/10.1002\/stem.2575.","journal-title":"Stem Cells"},{"key":"1566_CR35","doi-asserted-by":"publisher","unstructured":"Lee JH, Yoon YM, Lee SH. Hypoxic Preconditioning Promotes the Bioactivities of Mesenchymal Stem Cells via the HIF-1\u03b1-GRP78-Akt Axis. Int J Mol Sci [Internet]. 2017;18(6). https:\/\/doi.org\/10.3390\/ijms18061320.","DOI":"10.3390\/ijms18061320"},{"issue":"2","key":"1566_CR36","doi-asserted-by":"publisher","first-page":"63","DOI":"10.4021\/jocmr2009.03.1230","volume":"1","author":"A Dannan","year":"2009","unstructured":"Dannan A. Dental-derived stem cells and whole tooth regeneration: an overview. J Clin Med Res. 2009;1(2):63. https:\/\/doi.org\/10.4021\/jocmr2009.03.1230.","journal-title":"J Clin Med Res"},{"issue":"6","key":"1566_CR37","doi-asserted-by":"publisher","first-page":"633","DOI":"10.3390\/cells8060633","volume":"8","author":"B Widholz","year":"2019","unstructured":"Widholz B, Tsitlakidis S, Reible B, Moghaddam A, Westhauser F. Pooling of patient-derived mesenchymal stromal cells reduces inter-individual confounder-associated variation without negative impact on cell viability, proliferation and osteogenic differentiation. Cells. 2019;8(6):633. https:\/\/doi.org\/10.3390\/cells8060633.","journal-title":"Cells"},{"issue":"6","key":"1566_CR38","doi-asserted-by":"publisher","first-page":"5240","DOI":"10.1021\/acsami.7b17620","volume":"10","author":"W Li","year":"2018","unstructured":"Li W, Liu Y, Zhang P, Tang Y, Zhou M, Jiang W, et al. Tissue-Engineered Bone Immobilized with Human Adipose Stem Cells-Derived Exosomes Promotes Bone Regeneration. ACS Appl Mater Interfaces. 2018;10(6):5240\u201354. https:\/\/doi.org\/10.1021\/acsami.7b17620.","journal-title":"ACS Appl Mater Interfaces"},{"issue":"1suppl","key":"1566_CR39","doi-asserted-by":"publisher","first-page":"S","DOI":"10.1089\/pho.2009.2540","volume":"28","author":"W-T Li","year":"2010","unstructured":"Li W-T, Leu Y-C, Wu J-L. Red-Light Light-Emitting Diode Irradiation Increases the Proliferation and Osteogenic Differentiation of Rat Bone Marrow Mesenchymal Stem Cells. Photomed Laser Surg. 2010;28(1suppl):S\u2013157. https:\/\/doi.org\/10.1089\/pho.2009.2540.","journal-title":"Photomed Laser Surg"},{"key":"1566_CR40","doi-asserted-by":"publisher","unstructured":"Ohsugi Y, Niimi H, Shimohira T, Hatasa M, Katagiri S, Aoki A, et al. In Vitro Cytological Responses against Laser Photobiomodulation for Periodontal Regeneration. Int J Mol Sci. 2020;21(23). https:\/\/doi.org\/10.3390\/ijms21239002.","DOI":"10.3390\/ijms21239002"},{"key":"1566_CR41","doi-asserted-by":"publisher","first-page":"46746","DOI":"10.1038\/srep46746","volume":"7","author":"Z Chen","year":"2017","unstructured":"Chen Z, Zhang Q, Wang H, Li W, Wang F, Wan C, et al. Klf5 Mediates Odontoblastic Differentiation through Regulating Dentin-Specific Extracellular Matrix Gene Expression during Mouse Tooth Development. Sci Rep. 2017;7:46746. https:\/\/doi.org\/10.1038\/srep46746.","journal-title":"Sci Rep"},{"issue":"1","key":"1566_CR42","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1080\/15476278.2021.2022373","volume":"18","author":"S Chen","year":"2022","unstructured":"Chen S, Xie H, Zhao S, Wang S, Wei X, Liu S. The Genes Involved in Dentinogenesis. Organogenesis. 2022;18(1):1\u201319. https:\/\/doi.org\/10.1080\/15476278.2021.2022373.","journal-title":"Organogenesis"},{"issue":"1","key":"1566_CR43","doi-asserted-by":"publisher","first-page":"80","DOI":"10.1016\/j.bbrc.2013.11.055","volume":"443","author":"MJC Moester","year":"2014","unstructured":"Moester MJC, Schoeman MAE, Oudshoorn IB, van Beusekom MM, Mol IM, Kaijzel EL, et al. Validation of a simple and fast method to quantify in vitro mineralization with fluorescent probes used in molecular imaging of bone. Biochem Biophys Res Commun. 2014;443(1):80\u20135. https:\/\/doi.org\/10.1016\/j.bbrc.2013.11.055.","journal-title":"Biochem Biophys Res Commun"},{"key":"1566_CR44","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1016\/j.reth.2023.05.008","volume":"24","author":"J Zou","year":"2023","unstructured":"Zou J, Xia H, Jiang Q, Su Z, Wen S, Liang Z, et al. Exosomes derived from odontogenic stem cells: Its role in the dentin-pulp complex. Regen Ther. 2023;24:135\u201346. https:\/\/doi.org\/10.1016\/j.reth.2023.05.008.","journal-title":"Regen Ther"},{"key":"1566_CR45","doi-asserted-by":"publisher","unstructured":"Hertel FC, Silva AS, Sabino AD, Valente FL, Reis EC. Preconditioning Methods to Improve Mesenchymal Stromal Cell-Derived Extracellular Vesicles in Bone Regeneration\u2014A. Syst Rev Biology [Internet]. 2022;11(5). https:\/\/doi.org\/10.3390\/biology11050733.","DOI":"10.3390\/biology11050733"},{"issue":"9","key":"1566_CR46","doi-asserted-by":"publisher","first-page":"1112","DOI":"10.1177\/00220345261448514","volume":"105","author":"P Ahmad","year":"2026","unstructured":"Ahmad P, Estrin NE, Miron RJ. Extracellular Vesicles in Regenerative Dentistry. J Dent Res. 2026;105(9):1112\u201325. https:\/\/doi.org\/10.1177\/00220345261448514.","journal-title":"J Dent Res"},{"key":"1566_CR47","doi-asserted-by":"publisher","DOI":"10.1111\/prd.70054","author":"P Ahmad","year":"2026","unstructured":"Ahmad P, Sculean A, Miron RJ. Understanding exosomes in regenerative dentistry. Periodontol 2000. 2026. https:\/\/doi.org\/10.1111\/prd.70054.","journal-title":"Periodontol 2000"},{"issue":"1","key":"1566_CR48","doi-asserted-by":"publisher","first-page":"265","DOI":"10.1186\/s12951-024-02542-0","volume":"22","author":"H Lu","year":"2024","unstructured":"Lu H, Mu Q, Ku W, Zheng Y, Yi P, Lin L, et al. Functional extracellular vesicles from SHEDs combined with gelatin methacryloyl promote the odontogenic differentiation of DPSCs for pulp regeneration. J Nanobiotechnol. 2024;22(1):265. https:\/\/doi.org\/10.1186\/s12951-024-02542-0.","journal-title":"J Nanobiotechnol"},{"key":"1566_CR49","doi-asserted-by":"publisher","unstructured":"Ganesh V, Fredericks DC, Petersen EB, Keen HL, He R, Turner JD, et al. Extracellular Vesicles-Induced Cell Homing and Odontogenesis via microRNA Signaling for Dentin Regeneration. Int J Mol Sci. 2025;26(15). https:\/\/doi.org\/10.3390\/ijms26157182.","DOI":"10.3390\/ijms26157182"},{"issue":"11","key":"1566_CR50","doi-asserted-by":"publisher","first-page":"1978","DOI":"10.1002\/term.2947","volume":"13","author":"F Wei","year":"2019","unstructured":"Wei F, Li Z, Crawford R, Xiao Y, Zhou Y. Immunoregulatory role of exosomes derived from differentiating mesenchymal stromal cells on inflammation and osteogenesis. J Tissue Eng Regen Med. 2019;13(11):1978\u201391. https:\/\/doi.org\/10.1002\/term.2947.","journal-title":"J Tissue Eng Regen Med"},{"key":"1566_CR51","doi-asserted-by":"publisher","DOI":"10.1007\/s10266-025-01201-6","author":"E Hany","year":"2025","unstructured":"Hany E, Emam AA, Elzeiny D, Elzeiny AA, El-Qashty R. Comparison of the osteoinductive potential of preconditioned dental pulp stem cell secretome versus their exosomes. Odontology. 2025. https:\/\/doi.org\/10.1007\/s10266-025-01201-6.","journal-title":"Odontology"},{"key":"1566_CR52","doi-asserted-by":"publisher","unstructured":"Mussano F, Genova T, Petrillo S, Roato I, Ferracini R, Munaron L. Osteogenic Differentiation Modulates the Cytokine, Chemokine, and Growth Factor Profile of ASCs and SHED. Int J Mol Sci [Internet]. 2018;19(5). https:\/\/doi.org\/10.3390\/ijms19051454.","DOI":"10.3390\/ijms19051454"},{"issue":"3","key":"1566_CR53","doi-asserted-by":"publisher","first-page":"373","DOI":"10.1016\/j.joen.2008.11.028","volume":"35","author":"H Shiba","year":"2009","unstructured":"Shiba H, Tsuda H, Kajiya M, Fujita T, Takeda K, Hino T, et al. Neodymium-doped yttrium-aluminium-garnet laser irradiation abolishes the increase in interleukin-6 levels caused by peptidoglycan through the p38 mitogen-activated protein kinase pathway in human pulp cells. J Endod. 2009;35(3):373\u20136. https:\/\/doi.org\/10.1016\/j.joen.2008.11.028.","journal-title":"J Endod"},{"issue":"3","key":"1566_CR54","doi-asserted-by":"publisher","first-page":"331","DOI":"10.1007\/s10103-007-0491-5","volume":"23","author":"SM Safavi","year":"2008","unstructured":"Safavi SM, Kazemi B, Esmaeili M, Fallah A, Modarresi A, Mir M. Effects of low-level He-Ne laser irradiation on the gene expression of IL-1beta, TNF-alpha, IFN-gamma, TGF-beta, bFGF, and PDGF in rat\u2019s gingiva. Lasers Med Sci. 2008;23(3):331\u20135. https:\/\/doi.org\/10.1007\/s10103-007-0491-5.","journal-title":"Lasers Med Sci"},{"issue":"10","key":"1566_CR55","doi-asserted-by":"publisher","first-page":"762","DOI":"10.1089\/scd.2016.0332","volume":"26","author":"K Yin","year":"2017","unstructured":"Yin K, Zhu R, Wang S, Zhao RC. Low-Level Laser Effect on Proliferation, Migration, and Antiapoptosis of Mesenchymal Stem Cells. Stem Cells Dev. 2017;26(10):762\u201375. https:\/\/doi.org\/10.1089\/scd.2016.0332.","journal-title":"Stem Cells Dev"},{"issue":"1","key":"1566_CR56","doi-asserted-by":"publisher","first-page":"707","DOI":"10.1007\/s11033-025-10791-x","volume":"52","author":"ME Mota","year":"2025","unstructured":"Mota ME, Marques MM, Alves FA, Cecatto RB, Ariga SKK, Moreira MS. Priming effect of photobiomodulation of mesenchymal stem cells on extracellular vesicles for regenerative medicine. Mol Biol Rep. 2025;52(1):707. https:\/\/doi.org\/10.1007\/s11033-025-10791-x.","journal-title":"Mol Biol Rep"},{"key":"1566_CR57","doi-asserted-by":"publisher","first-page":"1131","DOI":"10.1007\/s10103-018-2495-8","volume":"33","author":"HS Bagheri","year":"2018","unstructured":"Bagheri HS, Mousavi M, Rezabakhsh A, Rezaie J, Rasta SH, Nourazarian A, et al. Low-level laser irradiation at a high power intensity increased human endothelial cell exosome secretion via Wnt signaling. Lasers Med Sci. 2018;33:1131\u201345. https:\/\/doi.org\/10.1007\/s10103-018-2495-8.","journal-title":"Lasers Med Sci"},{"key":"1566_CR58","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13287-019-1472-x","volume":"10","author":"K Yang","year":"2019","unstructured":"Yang K, Li D, Wang M, Xu Z, Chen X, Liu Q, et al. Exposure to blue light stimulates the proangiogenic capability of exosomes derived from human umbilical cord mesenchymal stem cells. Stem Cell Res Ther. 2019;10:1\u201314. https:\/\/doi.org\/10.1186\/s13287-019-1472-x.","journal-title":"Stem Cell Res Ther"},{"issue":"11","key":"1566_CR59","doi-asserted-by":"publisher","first-page":"4980","DOI":"10.55522\/jmpas.V11I3.3775","volume":"9","author":"LM Abdelgawad","year":"2022","unstructured":"Abdelgawad LM, Nghnughi MH, Abdelgwad M. Influence of photo biomodulation using 980 nm diode laser and exsosomes derived from dental pulp stem cells on pulp regeneration of dogs\u2019 teeth. Reactions. 2022;9(11):4980\u20135. https:\/\/doi.org\/10.55522\/jmpas.V11I3.3775.","journal-title":"Reactions"},{"issue":"1","key":"1566_CR60","doi-asserted-by":"publisher","first-page":"20","DOI":"10.20517\/evcna.2025.84","volume":"7","author":"V Miceli","year":"2026","unstructured":"Miceli V. Priming strategies to enhance the therapeutic efficacy of mesenchymal stromal\/stem cell-derived vesicles in regenerative medicine. Extracell Vesicles Circulating Nucleic Acids. 2026;7(1):20\u201333. https:\/\/doi.org\/10.20517\/evcna.2025.84.","journal-title":"Extracell Vesicles Circulating Nucleic Acids"},{"issue":"1","key":"1566_CR61","doi-asserted-by":"publisher","first-page":"7","DOI":"10.7518\/hxkq.2022.01.002","volume":"40","author":"L Liao","year":"2022","unstructured":"Liao L, Tian W. Prospect on the application of mesenchymal stem cell-derived extracellular vesicles in the regeneration of dental and maxillofacial tissues. Hua Xi Kou Qiang Yi Xue Za Zhi. 2022;40(1):7\u201313. https:\/\/doi.org\/10.7518\/hxkq.2022.01.002.","journal-title":"Hua Xi Kou Qiang Yi Xue Za Zhi"},{"issue":"2","key":"1566_CR62","doi-asserted-by":"publisher","first-page":"798","DOI":"10.3390\/ijms27020798","volume":"27","author":"B-H Chuah","year":"2026","unstructured":"Chuah B-H, Law J-X, Leong X-F, Pang K-L, Farm Y-R, Razali M, et al. Safety Assessment of Extracellular Vesicle-Based Therapy in Regenerative Dentistry. Int J Mol Sci. 2026;27(2):798. https:\/\/doi.org\/10.3390\/ijms27020798.","journal-title":"Int J Mol Sci"}],"container-title":["Odontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10266-026-01566-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10266-026-01566-2","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10266-026-01566-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T21:03:52Z","timestamp":1792530232000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10266-026-01566-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,4]]},"references-count":62,"alternative-id":["1566"],"URL":"https:\/\/doi.org\/10.1007\/s10266-026-01566-2","relation":{},"ISSN":["1618-1247","1618-1255"],"issn-type":[{"value":"1618-1247","type":"print"},{"value":"1618-1255","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,4]]},"assertion":[{"value":"8 June 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"4 September 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"4 October 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 October 2026","order":5,"name":"change_date","label":"Change Date","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"Update","order":6,"name":"change_type","label":"Change Type","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"Affiliation 4 has been updated.","order":7,"name":"change_details","label":"Change Details","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare that they have no conflict of interest.","order":1,"name":"Ethics","label":"Conflict of interest","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"This was an in vitro study conducted on human samples approved by the Ethical Committee of the faculty of dentistry, Mansoura University, Mansoura, Egypt with the IRP approval ID: R.25.09.20. All procedures were conducted in accordance with the ethical guidelines of the Declaration of Helsinki.","order":2,"name":"Ethics","label":"Ethical approval","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"For the isolation of human dental pulp stem cells, impacted third molars were extracted from patients attending the Oral Surgery department clinics at the Faculty of Dentistry, Mansoura University, Egypt, after acquiring informed consents in accordance with approval from the Ethical Committee of the faculty of dentistry, Mansoura University, Mansoura, Egypt and following the guidelines outlined by the Declaration of Helsinki.","order":3,"name":"Ethics","label":"Informed consent","group":{"name":"EthicsHeading","label":"Declarations"}}]}}