{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T12:34:12Z","timestamp":1789734852637,"version":"4.0.1"},"reference-count":34,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The focus of this research effort was to develop an autonomous, inducible, lux-based bioluminescent bioreporter for the real-time detection of dichloromethane. Dichloromethane (DCM), also known as methylene chloride, is a volatile organic compound and one of the most commonly used halogenated solvents in the U.S., with applications ranging from grease and paint stripping to aerosol propellants and pharmaceutical tablet coatings. Predictably, it is released into the environment where it contaminates air and water resources. Due to its classification as a probable human carcinogen, hepatic toxin, and central nervous system effector, DCM must be carefully monitored and controlled. Methods for DCM detection usually rely on analytical techniques such as solid-phase microextraction (SPME) and capillary gas chromatography or photoacoustic environmental monitors, all of which require trained personnel and\/or expensive equipment. To complement conventional monitoring practices, we have created a bioreporter for the self-directed detection of DCM by taking advantage of the evolutionary adaptation of bacteria to recognize and metabolize chemical agents. This bioreporter, Methylobacterium extorquens DCMlux, was engineered to contain a bioluminescent luxCDABE gene cassette derived from Photorhabdus luminescens fused downstream to the dcm dehalogenase operon, which causes the organism to generate visible light when exposed to DCM. We have demonstrated detection limits down to 1.0\u00a0ppm under vapor phase exposures and 0.1\u00a0ppm under liquid phase exposures with response times of 2.3 and 1.3\u00a0h, respectively, and with specificity towards DCM under relevant industrial environmental monitoring conditions.<\/jats:p>","DOI":"10.1007\/s10295-011-0997-5","type":"journal-article","created":{"date-parts":[[2011,6,18]],"date-time":"2011-06-18T07:06:50Z","timestamp":1308380810000},"page":"45-53","source":"Crossref","is-referenced-by-count":16,"title":["Detection of dichloromethane with a bioluminescent (<i>lux<\/i>) bacterial bioreporter"],"prefix":"10.1093","volume":"39","author":[{"given":"Nicholas","family":"Lopes","sequence":"first","affiliation":[{"name":"grid.411461.7 0000000123151184 The Center for Environmental Biotechnology University of Tennessee 676 Dabney Hall 37996 Knoxville TN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shawn A","family":"Hawkins","sequence":"additional","affiliation":[{"name":"grid.411461.7 0000000123151184 The Center for Environmental Biotechnology University of Tennessee 676 Dabney Hall 37996 Knoxville TN USA"},{"name":"grid.411461.7 0000000123151184 The Department of Biosystems Engineering and Soil Sciences University of Tennessee 676 Dabney Hall 37996 Knoxville TN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Jegier","sequence":"additional","affiliation":[{"name":"grid.411461.7 0000000123151184 The Center for Environmental Biotechnology University of Tennessee 676 Dabney Hall 37996 Knoxville TN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fu-Min","family":"Menn","sequence":"additional","affiliation":[{"name":"grid.411461.7 0000000123151184 The Center for Environmental Biotechnology University of Tennessee 676 Dabney Hall 37996 Knoxville TN USA"},{"name":"grid.135519.a 0000000404462659 The Joint Institute for Biological Sciences Oak Ridge National Laboratory 37831 Oak Ridge TN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary S","family":"Sayler","sequence":"additional","affiliation":[{"name":"grid.411461.7 0000000123151184 The Center for Environmental Biotechnology University of Tennessee 676 Dabney Hall 37996 Knoxville TN USA"},{"name":"grid.135519.a 0000000404462659 The Joint Institute for Biological Sciences Oak Ridge National Laboratory 37831 Oak Ridge TN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven","family":"Ripp","sequence":"additional","affiliation":[{"name":"grid.411461.7 0000000123151184 The Center for Environmental Biotechnology University of Tennessee 676 Dabney Hall 37996 Knoxville TN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2012,1,1]]},"reference":[{"key":"2021033103194512600_CR1","volume-title":"Toxicological profile for methylene 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