{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,18]],"date-time":"2025-10-18T15:01:05Z","timestamp":1760799665744},"reference-count":29,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2013,7,1]],"date-time":"2013-07-01T00:00:00Z","timestamp":1372636800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The neutral endo-\u03b2-glucanase gene cel5A from Humicola insolens was cloned and connected with the cellobiohydrolase 1 promoter from Trichoderma reesei to construct a recombinant plasmid pCB-hEG with the hygromycin B resistance marker. The plasmid was introduced into conidia of T. reesei using the Agrobacterium tumefaciens mediated transformation method. Eight transformants were obtained on screening plates with sodium carboxymethyl cellulose as the sole carbon source. Stable integration of the cel5A gene into the chromosomal DNA of T. reesei was confirmed by PCR. An obvious protein band (approximately 52\u00a0kDa) was detected by SDS-PAGE from fermentation broth, which showed that the cel5A gene in recombinant T. reesei successfully fulfilled efficient expression and extracellular secretion. After 96\u00a0h shaking-flask fermentation, the endo-\u03b2-glucanase activity at pH 6.5 from recombinant T. reesei reached 3,068\u00a0U\/ml, which was 11 times higher than that of the host strain. In a 2\u00a0m3 fermenter, the endo-\u03b2-glucanase activity could be further increased to 8,012\u00a0U\/ml after 96\u00a0h fermentation. The results showed a good prospect for application of neutral endo-\u03b2-glucanase in the textile industry.<\/jats:p>","DOI":"10.1007\/s10295-013-1267-5","type":"journal-article","created":{"date-parts":[[2013,4,30]],"date-time":"2013-04-30T21:23:48Z","timestamp":1367357028000},"page":"773-779","source":"Crossref","is-referenced-by-count":9,"title":["High expression of a neutral endo-\u03b2-glucanase gene from <i>Humicola insolens<\/i> in <i>Trichoderma reesei<\/i>"],"prefix":"10.1093","volume":"40","author":[{"given":"Bintao","family":"Gu","sequence":"first","affiliation":[{"name":"grid.13402.34 000000041759700X Key Laboratory of Biomass Chemical Engineering of Ministry of Education, Department of Chemical and Biological Engineering Zhejiang University 310027 Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liming","family":"Xia","sequence":"additional","affiliation":[{"name":"grid.13402.34 000000041759700X Key Laboratory of Biomass Chemical Engineering 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