{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T11:07:11Z","timestamp":1761217631393},"reference-count":44,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2013,8,1]],"date-time":"2013-08-01T00:00:00Z","timestamp":1375315200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Genome shuffling is an efficient approach for the rapid engineering of microbial strains with desirable industrial phenotypes. In this study, a strategy of incorporating streptomycin resistance screening into genome shuffling (GS-SR) was applied for rapid improvement of doramectin production by Streptomyces avermitilis NEAU1069. The starting mutant population was generated through treatment of the spores with N-methyl-N\u2019-nitro-N-nitrosoguanidine and ultraviolet (UV) irradiation, respectively, and five mutants with higher productivity of doramectin were selected as starting strains for GS-SR. Finally, a genetically stable strain F4-137 was obtained and characterized to be able to yield 992\u00a0\u00b1\u00a04.4\u00a0mg\/l doramectin in a shake flask, which was 7.3-fold and 11.2-fold higher than that of the starting strain UV-45 and initial strain NEAU1069, respectively. The doramectin yield by F4-137 in a 50-l fermentor reached 930.3\u00a0\u00b1\u00a03.8\u00a0mg\/l. Furthermore, the factors associated with the improved doramectin yield were investigated and the results suggested that mutations in ribosomal protein S12 and the enhanced production of cyclohexanecarboxylic coenzyme A may contribute to the improved performance of the shuffled strains. The random amplified polymorphic DNA analysis showed a genetic diversity among the shuffled strains, which confirmed the occurrence of genome shuffling. In conclusion, our results demonstrated that GS-SR is a powerful method for enhancing the production of secondary metabolites in Streptomyces.<\/jats:p>","DOI":"10.1007\/s10295-013-1280-8","type":"journal-article","created":{"date-parts":[[2013,5,8]],"date-time":"2013-05-08T17:01:18Z","timestamp":1368032478000},"page":"877-889","source":"Crossref","is-referenced-by-count":17,"title":["Streptomycin resistance-aided genome shuffling to improve doramectin productivity of <i>Streptomyces avermitilis<\/i> NEAU1069"],"prefix":"10.1093","volume":"40","author":[{"given":"Ji","family":"Zhang","sequence":"first","affiliation":[{"name":"grid.412243.2 0000000417601136 College of Life Science Northeast Agricultural University 150030 Harbin People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiangjing","family":"Wang","sequence":"additional","affiliation":[{"name":"grid.412243.2 0000000417601136 College of Life Science Northeast Agricultural University 150030 Harbin People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinna","family":"Diao","sequence":"additional","affiliation":[{"name":"grid.412243.2 0000000417601136 College of Life Science Northeast Agricultural University 150030 Harbin People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hairong","family":"He","sequence":"additional","affiliation":[{"name":"grid.412243.2 0000000417601136 College of Life Science Northeast Agricultural University 150030 Harbin People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuejing","family":"Zhang","sequence":"additional","affiliation":[{"name":"grid.412243.2 0000000417601136 College of Life Science Northeast Agricultural University 150030 Harbin People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wensheng","family":"Xiang","sequence":"additional","affiliation":[{"name":"grid.412243.2 0000000417601136 College of Life Science Northeast Agricultural University 150030 Harbin People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2013,8,1]]},"reference":[{"key":"2021033103200728300_CR1","doi-asserted-by":"publisher","first-page":"179","DOI":"10.1016\/j.mimet.2010.03.009","article-title":"Strain improvement of the pentose-fermenting yeast Pichia stipitis by genome shuffling","volume":"81","author":"Bajwa","year":"2010","journal-title":"J Microbiol Methods"},{"key":"2021033103200728300_CR2","doi-asserted-by":"publisher","first-page":"657","DOI":"10.1007\/s10295-010-0934-z","article-title":"Strain improvement in actinomycetes in the postgenomic era","volume":"38","author":"Baltz","year":"2011","journal-title":"J Ind Microbiol Biotechnol"},{"key":"2021033103200728300_CR3","doi-asserted-by":"publisher","first-page":"283","DOI":"10.1007\/s10295-005-0061-4","article-title":"Antibiotic production improvement in the rare actinomycete Planobispora rosea by selection of mutants resistant to the aminoglycosides Streptomycin and Gentamycin and to Rifamycin","volume":"33","author":"Beltrametti","year":"2006","journal-title":"J Ind Microbiol Biotechnol"},{"key":"2021033103200728300_CR4","doi-asserted-by":"publisher","first-page":"1857","DOI":"10.1007\/s11274-010-0477-5","article-title":"Genome shuffling of Hansenula anomala to improve flavour formation of soy sauce","volume":"28","author":"Cao","year":"2012","journal-title":"World J Microbiol Biotechnol"},{"key":"2021033103200728300_CR5","doi-asserted-by":"publisher","first-page":"2391","DOI":"10.1128\/AEM.70.4.2391-2397.2004","article-title":"Genome shuffling improves degradation of the anthropogenic pesticide pentachlorophenol by Sphingobium chlorophenolicum ATCC 39723","volume":"70","author":"Dai","year":"2004","journal-title":"Appl Environ Microbiol"},{"key":"2021033103200728300_CR6","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1007\/s10482-011-9551-8","article-title":"Genome shuffling of marine derived bacterium Nocardia sp. 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