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This buffer, however, has disadvantages: suspended CaCO3 particles make the medium turbid, thereby, obstructing analysis of microbial growth via optical density and scattered light. Upon searching for alternative soluble pH-buffers, bacterial growth and productivity was inhibited most probably due to osmotic stress. Thus, this study investigates in detail the osmotic sensitivity of G. oxydans ATCC\u00a0621H and DSM\u00a03504 using the Respiratory Activity MOnitoring System. The tested soluble pH-buffers and other salts attained osmolalities of 0.32\u20131.19\u00a0osmol\u00a0kg\u22121. This study shows that G. oxydans ATCC 621H and DSM 3504 respond quite sensitively to increased osmolality in comparison to other microbial strains of industrial interest. Osmolality values of &amp;gt;0.5\u00a0osmol\u00a0kg\u22121 should not be exceeded to avoid inhibition of growth and product formation. This osmolality threshold needs to be considered when working with soluble pH-buffers.<\/jats:p>","DOI":"10.1007\/s10295-015-1588-7","type":"journal-article","created":{"date-parts":[[2015,2,2]],"date-time":"2015-02-02T11:22:49Z","timestamp":1422876169000},"page":"585-600","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Quantifying the sensitivity of <i>G. oxydans<\/i> ATCC\u00a0621H and DSM 3504 to osmotic stress triggered by soluble buffers"],"prefix":"10.1093","volume":"42","author":[{"given":"B","family":"Luchterhand","sequence":"first","affiliation":[{"name":"grid.1957.a 000000010728696X AVT, Chair of Biochemical Engineering RWTH Aachen University Worringerweg 1 52074 Aachen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Fisch\u00f6der","sequence":"additional","affiliation":[{"name":"grid.1957.a 000000010728696X Department of Biotechnology\/Biomaterial Sciences and Helmholtz Institute for Biomedical Engineering RWTH Aachen University Pauwelsstr. 20 52074 Aachen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A R","family":"Grimm","sequence":"additional","affiliation":[{"name":"grid.1957.a 000000010728696X Institute of Biotechnology RWTH Aachen University Worringerweg 3 52074 Aachen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Wewetzer","sequence":"additional","affiliation":[{"name":"grid.1957.a 000000010728696X AVT, Chair of Biochemical Engineering RWTH Aachen University Worringerweg 1 52074 Aachen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Wunderlich","sequence":"additional","affiliation":[{"name":"grid.1957.a 000000010728696X AVT, Chair of Biochemical Engineering RWTH Aachen University Worringerweg 1 52074 Aachen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Schlep\u00fctz","sequence":"additional","affiliation":[{"name":"grid.1957.a 000000010728696X AVT, Chair of Biochemical Engineering RWTH Aachen University Worringerweg 1 52074 Aachen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"B\u00fcchs","sequence":"additional","affiliation":[{"name":"grid.1957.a 000000010728696X AVT, Chair of Biochemical Engineering RWTH Aachen University Worringerweg 1 52074 Aachen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2015,4,1]]},"reference":[{"issue":"2","key":"2021033104014826800_CR1","doi-asserted-by":"publisher","first-page":"179","DOI":"10.1016\/0014-5793(81)81114-3","article-title":"Existence of a novel prosthetic group, PQQ, in membrane-bound, electron transport chain-linked, primary dehydrogenases of oxidative bacteria","volume":"130","author":"Ameyama","year":"1981","journal-title":"FEBS Lett"},{"issue":"2","key":"2021033104014826800_CR2","doi-asserted-by":"publisher","first-page":"157","DOI":"10.1016\/S1369-703X(00)00116-9","article-title":"Device for sterile online measurement of the oxygen transfer rate in shaking flasks","volume":"7","author":"Anderlei","year":"2001","journal-title":"Biochem Eng J"},{"issue":"5","key":"2021033104014826800_CR3","doi-asserted-by":"publisher","first-page":"999","DOI":"10.1002\/bit.21490","article-title":"New method to determine the mass transfer resistance of sterile closures for shaken bioreactors","volume":"98","author":"Anderlei","year":"2007","journal-title":"Biotechnol Bioeng"},{"issue":"3","key":"2021033104014826800_CR4","doi-asserted-by":"publisher","first-page":"187","DOI":"10.1016\/S1369-703X(03)00181-5","article-title":"Online respiration activity measurement (OTR, CTR, RQ) in shake flasks","volume":"17","author":"Anderlei","year":"2004","journal-title":"Biochem Eng J"},{"key":"2021033104014826800_CR5","volume-title":"Acetic Acid Bacteria: Classification and Biochemical Activities","author":"Asai","year":"1968"},{"key":"2021033104014826800_CR6","doi-asserted-by":"publisher","first-page":"301","DOI":"10.1007\/BF00369561","article-title":"Some kinetic aspects and modeling of biotransformation of d-glucose to keto-d-gluconates","volume":"13","author":"Beschkov","year":"1995","journal-title":"Bioprocess Eng"},{"key":"2021033104014826800_CR7","volume-title":"Microbial water stress physiology: principles and perspectives","author":"Brown","year":"1990"},{"issue":"9","key":"2021033104014826800_CR8","doi-asserted-by":"publisher","first-page":"2503","DOI":"10.1021\/bi00512a022","article-title":"Role of metal cofactors in enzyme regulation. 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