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Est12 prefers short- and middle-chain p-nitrophenol esters as substrate with optimum temperature and pH value of 50\u00a0\u00b0C and 9.0, respectively, and nearly 50\u00a0% of maximum activity retained at 5\u00a0\u00b0C. The hydrolysis activity of Est12 was stable at 40\u00a0\u00b0C. Ca2+ especially activated the activity of Est12 to about 151\u00a0% of the control. DEPC and PMSF inhibited the activity of Est12 to 34 and 25\u00a0%, respectively. In addition, Est12 was more tolerable to methanol compared to other organic solvents tested. The crystal structure of Est12 at 1.39 \u00c5 resolution showed that the cap domain which is composed of an \u03b1-helix and a flexible region resulted in a relatively wide spectrum of substrate, with p-nitrophenol caproate as the preferred one. 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