{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T06:38:16Z","timestamp":1774420696934,"version":"3.50.1"},"reference-count":39,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2015,11,1]],"date-time":"2015-11-01T00:00:00Z","timestamp":1446336000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"},{"start":{"date-parts":[[2015,11,1]],"date-time":"2015-11-01T00:00:00Z","timestamp":1446336000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"crossref","award":["NRF-2014R1A2A2A01005461"],"award-info":[{"award-number":["NRF-2014R1A2A2A01005461"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Streptomyces sp. M10 is a valinomycin-producing bacterial strain that shows potent bioactivity against Botrytis blight of cucumber plants. During studies to increase the yield of valinomycin (a cyclododecadepsipeptide) in strain M10, additional antifungal metabolites, including bafilomycin derivatives (macrolide antibiotics), were identified. To examine the effect of bafilomycin biosynthesis on valinomycin production, the bafilomycin biosynthetic gene cluster was cloned from the genome of strain M10, as were two branched-chain \u03b1-keto acid dehydrogenase (BCDH) gene clusters related to precursor supply for bafilomycin biosynthesis. A null mutant (M10bafm) of one bafilomycin biosynthetic gene (bafV) failed to produce bafilomycin, but resulted in a 1.2- to 1.5-fold increase in the amount of valinomycin produced. In another null mutant (M10bkdFm) of a gene encoding a subunit of the BCDH complex (bkdF), bafilomycin production was completely abolished and valinomycin production increased fourfold relative to that in the wild-type M10 strain. The higher valinomycin yield was likely the result of redistribution of the metabolic flux from bafilomycin to valinomycin biosynthesis, because the two antibiotics share a common precursor, 2-ketoisovaleric acid, a deamination product of valine. The results show that directing precursor flux toward active ingredient biosynthesis could be used as a prospective tool to increase the competence of biofungicides.<\/jats:p>","DOI":"10.1007\/s10295-015-1679-5","type":"journal-article","created":{"date-parts":[[2015,9,2]],"date-time":"2015-09-02T07:55:03Z","timestamp":1441180503000},"page":"1507-1517","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Increased valinomycin production in mutants of <i>Streptomyces<\/i> sp. M10 defective in bafilomycin biosynthesis and branched-chain \u03b1-keto acid dehydrogenase complex expression"],"prefix":"10.1093","volume":"42","author":[{"given":"Dong Wan","family":"Lee","sequence":"first","affiliation":[{"name":"grid.222754.4 0000000108402678 Plant Pharmacology Laboratory, Department of Biosystems and Biotechnology Korea University Graduate School 136-713 Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bee Gek","family":"Ng","sequence":"additional","affiliation":[{"name":"grid.222754.4 0000000108402678 Division of Biotechnology, College of Life Sciences and Biotechnology Korea University 136-713 Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beom Seok","family":"Kim","sequence":"additional","affiliation":[{"name":"grid.222754.4 0000000108402678 Plant Pharmacology Laboratory, Department of Biosystems and Biotechnology Korea University Graduate School 136-713 Seoul Republic of Korea"},{"name":"grid.222754.4 0000000108402678 Division of Biotechnology, College of Life Sciences and Biotechnology Korea University 136-713 Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2015,11,1]]},"reference":[{"key":"2021033104013730000_CR1"},{"key":"2021033104013730000_CR2","doi-asserted-by":"publisher","first-page":"7972","DOI":"10.1073\/pnas.85.21.7972","article-title":"Bafilomycins: a class of inhibitors of membrane ATPases from microorganisms, animal cells, and plant cells","volume":"85","author":"Bowman","year":"1988","journal-title":"Proc Natl Acad Sci USA"},{"key":"2021033104013730000_CR3","doi-asserted-by":"publisher","first-page":"287","DOI":"10.1046\/j.1365-2958.2003.03442.x","article-title":"The Lrp family of transcriptional regulators","volume":"48","author":"Brinkman","year":"2003","journal-title":"Mol Microbiol"},{"key":"2021033104013730000_CR4","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1007\/s00253-009-2428-3","article-title":"Improvement of secondary metabolite production in Streptomyces by manipulating pathway regulation","volume":"86","author":"Chen","year":"2010","journal-title":"Appl Microbiol Biotechnol"},{"key":"2021033104013730000_CR5","first-page":"471","article-title":"Deciphering the biosynthetic codes for the potent anti-SARS-CoV cyclodepsipeptide valinomycin in streptomyces tsusimaensis ATCC 15141","volume":"7","author":"Cheng","year":"2006","journal-title":"Chem Biochem"},{"key":"2021033104013730000_CR6","doi-asserted-by":"publisher","first-page":"524","DOI":"10.1002\/ps.1378","article-title":"Natural products that have been used commercially as crop protection agents","volume":"63","author":"Copping","year":"2007","journal-title":"Pest Manag Sci"},{"key":"2021033104013730000_CR7","doi-asserted-by":"publisher","first-page":"1602","DOI":"10.1007\/s12010-013-0418-5","article-title":"Isolation and characterization of phytotoxic compounds produced by Streptomyces sp. 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