{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T04:09:26Z","timestamp":1789445366107,"version":"build-2803163510"},"reference-count":25,"publisher":"Oxford University Press (OUP)","issue":"2-3","license":[{"start":{"date-parts":[[2016,3,1]],"date-time":"2016-03-01T00:00:00Z","timestamp":1456790400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"},{"start":{"date-parts":[[2016,3,1]],"date-time":"2016-03-01T00:00:00Z","timestamp":1456790400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"funder":[{"DOI":"10.13039\/100015613","name":"Dow AgroSciences LLC","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100015613","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>This review highlights the importance of natural product research and industrial microbiology for product development in the agricultural industry, based on examples from Dow AgroSciences. It provides an overview of the discovery and development of spinetoram, a semisynthetic insecticide derived by a combination of a genetic block in a specific O-methylation of the rhamnose moiety of spinosad coupled with neural network-based QSAR and synthetic chemistry. It also emphasizes the key role that new technologies and multidisciplinary approaches play in the development of current spinetoram production strains.<\/jats:p>","DOI":"10.1007\/s10295-015-1710-x","type":"journal-article","created":{"date-parts":[[2015,11,18]],"date-time":"2015-11-18T06:27:40Z","timestamp":1447828060000},"page":"185-193","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":74,"title":["Natural product derived insecticides: discovery and development of spinetoram"],"prefix":"10.1093","volume":"43","author":[{"given":"Ute","family":"Galm","sequence":"first","affiliation":[{"name":"grid.418574.b 0000000121793263 Dow AgroSciences LLC 9330 Zionsville Road 46268 Indianapolis IN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas C","family":"Sparks","sequence":"additional","affiliation":[{"name":"grid.418574.b 0000000121793263 Dow AgroSciences LLC 9330 Zionsville Road 46268 Indianapolis IN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2016,3,1]]},"reference":[{"key":"2023072014375490000_CR1","doi-asserted-by":"publisher","first-page":"2824","DOI":"10.1021\/jm00113a022","article-title":"Applications of neural networks in quantitative structure-activity relationships of dihydrofolate reductase inhibitors","volume":"34","author":"Andrea","year":"1991","journal-title":"J Med Chem"},{"key":"2023072014375490000_CR2"},{"key":"2023072014375490000_CR3","doi-asserted-by":"publisher","first-page":"177","DOI":"10.1002\/1526-4998(200102)57:2&lt;177::AID-PS281&gt;3.0.CO;2-Z","article-title":"Recent advances in the chemistry of spinosyns","volume":"57","author":"Crouse","year":"2001","journal-title":"Pest Manag Sci"},{"key":"2023072014375490000_CR4","doi-asserted-by":"publisher","first-page":"1705","DOI":"10.1039\/b900233b","article-title":"Glycosylation engineering of spinosyn analogues containing an L-olivose moiety","volume":"7","author":"Gaisser","year":"2009","journal-title":"Org Biomol Chem"},{"key":"2023072014375490000_CR5","doi-asserted-by":"crossref","DOI":"10.1039\/b200536k","article-title":"Engineered biosynthesis of novel spinosyns bearing altered deoxyhexose substituents","author":"Gaisser","year":"2002","journal-title":"Chem Commun"},{"key":"2023072014375490000_CR6"},{"key":"2023072014375490000_CR7","doi-asserted-by":"publisher","first-page":"1169","DOI":"10.1002\/ps.3744","article-title":"Natural products for pest control: an analysis of their role, value and future","volume":"70","author":"Gerwick","year":"2014","journal-title":"Pest Manag Sci"},{"key":"2023072014375490000_CR8","doi-asserted-by":"publisher","first-page":"812","DOI":"10.1126\/science.1185383","article-title":"Food security: the challenge of feeding 9 billion people","volume":"327","author":"Godfray","year":"2010","journal-title":"Science"},{"key":"2023072014375490000_CR9","doi-asserted-by":"publisher","first-page":"94","DOI":"10.1007\/s10295-005-0016-9","article-title":"Butenyl-spinosyns, a natural example of genetic engineering of antibiotic biosynthetic genes","volume":"33","author":"Hahn","year":"2006","journal-title":"J Ind Microbiol Biotechnol"},{"key":"2023072014375490000_CR10"},{"key":"2023072014375490000_CR11"},{"key":"2023072014375490000_CR12","doi-asserted-by":"publisher","first-page":"14582","DOI":"10.1021\/ja076580i","article-title":"The biosynthesis of spinosyn in Saccharopolyspora spinosa: synthesis of the cross-bridging precursor and identification of the function of SpnJ","volume":"129","author":"Kim","year":"2007","journal-title":"J Am Chem Soc"},{"key":"2023072014375490000_CR13","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1038\/nature09981","article-title":"Enzyme-catalysed [4+2] cycloaddition is a key step in the biosynthesis of spinosyn A","volume":"473","author":"Kim","year":"2011","journal-title":"Nature"},{"key":"2023072014375490000_CR14","doi-asserted-by":"publisher","first-page":"2901","DOI":"10.1021\/ja910223x","article-title":"Biosynthesis of spinosyn in Saccharopolyspora spinosa: synthesis of permethylated rhamnose and characterization of the functions of SpnH, SpnI, and SpnK","volume":"132","author":"Kim","year":"2010","journal-title":"J Am Chem Soc"},{"key":"2023072014375490000_CR15","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1038\/ja.2010.5","article-title":"The spinosyn family of insecticides: realizing the potential of natural products research","volume":"63","author":"Kirst","year":"2010","journal-title":"J Antibiot"},{"key":"2023072014375490000_CR16","doi-asserted-by":"publisher","first-page":"4839","DOI":"10.1016\/S0040-4039(00)93474-9","article-title":"A83543A-D, unique fermentation-derived tetracyclic macrolides","volume":"32","author":"Kirst","year":"1991","journal-title":"Tetrahedron Lett"},{"key":"2023072014375490000_CR17","doi-asserted-by":"publisher","first-page":"399","DOI":"10.1038\/sj.jim.7000180","article-title":"Genes for the biosynthesis of spinosyns: applications for yield improvement in Saccharopolyspora spinosa","volume":"27","author":"Madduri","year":"2001","journal-title":"J Ind Microbiol Biotechnol"},{"key":"2023072014375490000_CR18","doi-asserted-by":"publisher","first-page":"5632","DOI":"10.1128\/JB.183.19.5632-5638.2001","article-title":"Rhamnose biosynthesis pathway supplies precursors for primary and secondary metabolism in Saccharopolyspora spinosa","volume":"183","author":"Madduri","year":"2001","journal-title":"J Bacteriol"},{"key":"2023072014375490000_CR19","doi-asserted-by":"publisher","first-page":"34","DOI":"10.1099\/00207713-40-1-34","article-title":"Saccharopolyspora spinosa sp. nov. isolated from soil collected in a sugar mill rum still","volume":"40","author":"Mertz","year":"1990","journal-title":"Int J Syst Bacteriol"},{"key":"2023072014375490000_CR20","doi-asserted-by":"publisher","first-page":"e66428","DOI":"10.1371\/journal.pone.0066428","article-title":"Yield trends are insufficient to double global crop production by 2050","volume":"8","author":"Ray","year":"2013","journal-title":"PLoS ONE"},{"key":"2023072014375490000_CR21","doi-asserted-by":"publisher","first-page":"1702","DOI":"10.1021\/np0602517","article-title":"Engineering of the spinosyn PKS: directing starter unit incorporation","volume":"69","author":"Sheehan","year":"2006","journal-title":"J Nat Prod"},{"key":"2023072014375490000_CR22","doi-asserted-by":"publisher","first-page":"187","DOI":"10.1006\/pest.2000.2490","article-title":"The application of artificial neural networks to the identification of new spinosoids with improved biological activity toward larvae of Heliothis virescens","volume":"67","author":"Sparks","year":"2000","journal-title":"Pestic Biochem Physiol"},{"key":"2023072014375490000_CR23","doi-asserted-by":"publisher","first-page":"393","DOI":"10.1007\/s10822-008-9205-8","article-title":"Neural network-based QSAR and insecticide discovery: spinetoram","volume":"22","author":"Sparks","year":"2008","journal-title":"J Comput Aid Mol Des"},{"key":"2023072014375490000_CR24","doi-asserted-by":"publisher","first-page":"487","DOI":"10.1016\/S1074-5521(01)00029-1","article-title":"Cloning and analysis of the spinosad biosynthetic gene cluster of Saccharopolyspora spinosa","volume":"8","author":"Waldron","year":"2001","journal-title":"Chem Biol"},{"key":"2023072014375490000_CR25","volume-title":"Neural Networks for chemists","author":"Zupan","year":"2000","edition":"2"}],"container-title":["Journal of Industrial Microbiology and Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s10295-015-1710-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s10295-015-1710-x\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/jimb\/article-pdf\/43\/2-3\/185\/50916232\/jimb0185.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jimb\/article-pdf\/43\/2-3\/185\/50916232\/jimb0185.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,20]],"date-time":"2023-07-20T16:31:34Z","timestamp":1689870694000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jimb\/article\/43\/2-3\/185\/5995758"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,3,1]]},"references-count":25,"journal-issue":{"issue":"2-3","published-online":{"date-parts":[[2016,3,1]]},"published-print":{"date-parts":[[2016,3,1]]}},"URL":"https:\/\/doi.org\/10.1007\/s10295-015-1710-x","relation":{},"ISSN":["1476-5535","1367-5435"],"issn-type":[{"value":"1476-5535","type":"electronic"},{"value":"1367-5435","type":"print"}],"subject":[],"published-other":{"date-parts":[[2016,3,1]]},"published":{"date-parts":[[2016,3,1]]}}}