{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T17:57:05Z","timestamp":1778003825886,"version":"3.51.4"},"reference-count":34,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2016,4,1]],"date-time":"2016-04-01T00:00:00Z","timestamp":1459468800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"},{"start":{"date-parts":[[2016,4,1]],"date-time":"2016-04-01T00:00:00Z","timestamp":1459468800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Modification of enzymes involved in transcription- or translation-processes is an interesting way to increase secondary metabolite production in Streptomycetes. However, application of such methods has not been widely described for strains which produce nucleoside antibiotics. The nucleoside antibiotic toyocamycin (TM) is produced by Streptomyces diastatochromogenes 1628. For improving TM production in S. diastatochromogenes 1628, the strain was spread on rifamycin-resistant (Rifr) medium. Several spontaneous mutants were obtained with mutations in the rpoB gene which encodes a RNA polymerase \u03b2-subunit. The mutants which showed increased TM production were detected at a frequency of 7.5\u00a0% among the total Rifr mutants. Mutant 1628-T15 harboring amino acid substitution His437Arg was the best TM producer with a 4.5-fold increase in comparison to that of the wild-type strain. The worst producer was mutant 1628-T62 which also showed a poor sporulation behavior. RT-PCR was performed to study the transcription levels of the TM biosynthetic gene toyG in the parental strain as well as in mutants 1628-T15 and 1628-T62. The transcriptional level of toyG was higher in mutant 1628-T15 than that in parental strain 1628, while much lower in mutant 1628-T62. In mutant strain 1628-T62 the expression of adpA \u00a0sd gene, which is required for morphological differentiation, was also much lower. Our studies also indicate that the introduction of mutations into rpoB is an effective strategy to improve the production of TM which is an important nucleoside antibiotic.<\/jats:p>","DOI":"10.1007\/s10295-015-1732-4","type":"journal-article","created":{"date-parts":[[2016,1,20]],"date-time":"2016-01-20T09:10:04Z","timestamp":1453281004000},"page":"463-471","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Characterization of representative <i>rpoB<\/i> gene mutations leading to a significant change in toyocamycin production of <i>Streptomyces diastatochromogenes<\/i> 1628"],"prefix":"10.1093","volume":"43","author":[{"given":"Zheng","family":"Ma","sequence":"first","affiliation":[{"name":"grid.411485.d 0000000417551108 Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, College of Life Sciences China Jiliang University 310018 Hangzhou Zhejiang Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuai","family":"Luo","sequence":"additional","affiliation":[{"name":"grid.411485.d 0000000417551108 Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, College of Life Sciences China Jiliang University 310018 Hangzhou Zhejiang Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xianhao","family":"Xu","sequence":"additional","affiliation":[{"name":"grid.411485.d 0000000417551108 Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, College of Life Sciences China Jiliang University 310018 Hangzhou Zhejiang Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreas","family":"Bechthold","sequence":"additional","affiliation":[{"name":"grid.5963.9 Pharmazeutische Biologie und Biotechnologie, Institut f\u00fcr Pharmazeutische Wissenschaften Albert-Ludwigs-Universit\u00e4t Freiburg im Breisgau 19 Stefan-Meier-Stra\u00dfe 79104 Freiburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoping","family":"Yu","sequence":"additional","affiliation":[{"name":"grid.411485.d 0000000417551108 Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, College of Life Sciences China Jiliang University 310018 Hangzhou Zhejiang Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2016,4,1]]},"reference":[{"issue":"9","key":"2021033104404653300_CR1","doi-asserted-by":"publisher","first-page":"619","DOI":"10.1038\/ja.2014.105","article-title":"Spontaneous and induced mutations to rifampicin, streptomycin and spectinomycin resistances in actinomycetes: mutagenic mechanisms and applications for strain improvement","volume":"67","author":"Baltz","year":"2014","journal-title":"J Antibiot 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